##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780590_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 28172 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.4054735198069 34.0 32.0 34.0 24.0 34.0 2 32.48612097117706 34.0 34.0 34.0 31.0 34.0 3 32.99929007525202 34.0 34.0 34.0 31.0 34.0 4 33.138257844668466 34.0 34.0 34.0 32.0 34.0 5 33.184260968337355 34.0 34.0 34.0 32.0 34.0 6 36.65692886554025 38.0 37.0 38.0 34.0 38.0 7 36.275734772114156 38.0 37.0 38.0 34.0 38.0 8 36.04188556013062 38.0 37.0 38.0 32.0 38.0 9 35.97795683657532 38.0 36.0 38.0 32.0 38.0 10-11 35.42758767570638 37.5 35.5 38.0 29.0 38.0 12-13 35.376331108902455 37.0 36.0 38.0 31.0 38.0 14-15 35.66631762033225 37.5 36.0 38.0 31.5 38.0 16-17 35.903858441005255 38.0 36.0 38.0 32.0 38.0 18-19 35.834818259264516 38.0 36.0 38.0 31.5 38.0 20-21 35.58691253727105 38.0 36.0 38.0 31.0 38.0 22-23 35.48463012920631 37.5 35.5 38.0 29.0 38.0 24-25 34.59365682237683 37.0 34.5 38.0 25.5 38.0 26-27 34.86614368876899 37.0 34.5 38.0 27.0 38.0 28-29 34.67055942070141 37.0 34.5 38.0 26.5 38.0 30-31 34.204280846230304 37.0 34.0 38.0 25.0 38.0 32-33 34.80837356240238 37.0 34.5 38.0 27.0 38.0 34-35 34.83728524776374 37.0 34.5 38.0 27.5 38.0 36-37 35.1370332244782 37.0 35.5 38.0 29.0 38.0 38-39 34.895783046997025 37.0 35.0 38.0 28.5 38.0 40-41 33.9999822518813 37.0 34.0 38.0 25.0 38.0 42-43 34.451245917932695 37.0 34.0 38.0 25.5 38.0 44-45 34.84546713048417 37.0 34.5 38.0 27.0 38.0 46-47 35.061887689904864 37.0 35.0 38.0 27.0 38.0 48-49 34.704138861280704 37.0 34.5 38.0 26.5 38.0 50-51 35.138240096549765 37.0 35.5 38.0 29.0 38.0 52-53 34.9117741019452 37.0 35.0 38.0 27.0 38.0 54-55 35.03460883146386 37.0 35.0 38.0 27.0 38.0 56-57 35.162963225898054 37.0 35.5 38.0 29.0 38.0 58-59 35.3719828198211 37.0 36.0 38.0 31.0 38.0 60-61 34.77154621610109 37.0 35.0 38.0 27.5 38.0 62-63 35.10263737043873 37.0 35.5 38.0 28.5 38.0 64-65 35.24474655686497 37.0 36.0 38.0 30.0 38.0 66-67 34.366622887973875 37.0 34.0 38.0 25.0 38.0 68-69 33.7012104216953 37.0 34.0 38.0 23.5 38.0 70-71 33.70637512423683 37.0 34.0 38.0 24.0 38.0 72-73 33.317939798381374 37.0 34.0 38.0 22.0 38.0 74-75 34.35513985517535 37.0 34.0 38.0 26.0 38.0 76 33.776054238250744 37.0 34.0 38.0 24.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.10754154954056006 1101 2 0.8692185490092612 1101 3 -0.06282075887649796 1101 4 0.34410828858432296 1101 5 0.22955286607113123 1101 6 -1.904278796692978 1101 7 0.6613402517719322 1101 8 1.005166726619457 1101 9 0.7701520088024338 1101 10-11 0.2582076622291396 1101 12-13 1.1750072000344005 1101 14-15 1.0345190218037033 1101 16-17 0.2770234531300204 1101 18-19 -0.18671001950747979 1101 20-21 -0.19258043810516057 1101 22-23 1.226195521804975 1101 24-25 -2.4980587784720925 1101 26-27 -0.6972844645320109 1101 28-29 0.714441011469205 1101 30-31 1.4049659567068673 1101 32-33 0.1724420210526958 1101 34-35 -0.10095386788817251 1101 36-37 -0.12428529226768603 1101 38-39 0.15694742363725567 1101 40-41 1.7656395380310812 1101 42-43 0.903850467624018 1101 44-45 0.19529783069150142 1101 46-47 0.7121084225480558 1101 48-49 -1.6750523401058572 1101 50-51 -0.8140052755351377 1101 52-53 -2.6198999994943364 1101 54-55 -1.2873686453307158 1101 56-57 -0.8302522345453056 1101 58-59 -1.1060863698260945 1101 60-61 -2.7581175572721186 1101 62-63 -1.5753815591314648 1101 64-65 -2.060629930967984 1101 66-67 -1.5929178583806873 1101 68-69 -1.4354771442827925 1101 70-71 -0.7438055009831857 1101 72-73 -2.9802295389222095 1101 74-75 -2.279328278322012 1101 76 -2.000837590113907 1102 1 0.39119746351905604 1102 2 0.7479282264286127 1102 3 0.9377168755321037 1102 4 0.8355061380466893 1102 5 0.46471415639371827 1102 6 0.5922803430919643 1102 7 0.11564132704074837 1102 8 1.387209737372146 1102 9 0.7317649120282397 1102 10-11 0.48890658696032574 1102 12-13 1.4051684903569779 1102 14-15 0.450002892771451 1102 16-17 0.49390517356012253 1102 18-19 0.22253729232047448 1102 20-21 0.497365798453977 1102 22-23 0.9599051992243304 1102 24-25 -4.116714692450586 1102 26-27 -0.6614242494782516 1102 28-29 0.061752839426198136 1102 30-31 2.2957186448789173 1102 32-33 0.6933559995473217 1102 34-35 1.424368712756987 1102 36-37 -2.461220776138653 1102 38-39 -0.09488053335198998 1102 40-41 1.7575750219020492 1102 42-43 1.5486891773014406 1102 44-45 1.5061042823044062 1102 46-47 1.8436675623329961 1102 48-49 -2.6561813723639247 1102 50-51 0.3422312836046544 1102 52-53 -0.37360967691369495 1102 54-55 1.1889754406907898 1102 56-57 1.4469520665299598 1102 58-59 0.4143975011416501 1102 60-61 -6.197848740067819 1102 62-63 -2.7798976881637216 1102 64-65 1.2549614668814755 1102 66-67 1.6727273029096352 1102 68-69 0.12188844711505453 1102 70-71 -0.012622705284258018 1102 72-73 -6.378186528169522 1102 74-75 -2.1503497836983527 1102 76 0.5783021948323324 1103 1 1.1978641301857245 1103 2 1.241261559761945 1103 3 0.19605020886543656 1103 4 0.6855061380466907 1103 5 0.7080474897270506 1103 6 0.6489470097586363 1103 7 1.4823079937074155 1103 8 1.743876404038815 1103 9 0.7967649120282445 1103 10-11 0.9747399202936577 1103 12-13 -0.5673315096430258 1103 14-15 -0.30499710722855866 1103 16-17 0.6414051735601234 1103 18-19 1.0775372923204714 1103 20-21 0.6798657984539744 1103 22-23 1.148238532557663 1103 24-25 -2.709214692450587 1103 26-27 0.13524241718841523 1103 28-29 -0.184080493907139 1103 30-31 1.7948853115455776 1103 32-33 2.0283559995473226 1103 34-35 0.6518687127569862 1103 36-37 -0.9745541094719883 1103 38-39 0.4576194666480049 1103 40-41 0.5409083552353806 1103 42-43 0.7711891773014443 1103 44-45 1.8544376156377425 1103 46-47 1.398667562333003 1103 48-49 -2.1661813723639227 1103 50-51 -0.08443538306201503 1103 52-53 0.7705569897529685 1103 54-55 -0.33185789264254595 1103 56-57 0.5244520665299532 1103 58-59 0.49189750114165065 1103 60-61 -4.262015406734484 1103 62-63 -1.1065643548303896 1103 64-65 0.15079480021480407 1103 66-67 -0.3122726970903642 1103 68-69 0.1643884471150585 1103 70-71 0.6498772947157434 1103 72-73 -4.137353194836187 1103 74-75 -2.123683117031689 1103 76 -5.041697805167672 1104 1 0.42791877499446684 1104 2 1.134157734625333 1104 3 0.6696840886468536 1104 4 0.24698154788275417 1104 5 -0.6662694501636608 1104 6 0.34867378571491514 1104 7 1.160723294253863 1104 8 0.8411441636016548 1104 9 1.4020927808807002 1104 10-11 -1.3272409540232815 1104 12-13 0.2422996378979576 1104 14-15 1.1503307616239056 1104 16-17 0.1399707473306151 1104 18-19 0.765652046418829 1104 20-21 0.25630022468348557 1104 22-23 0.40097077299482464 1104 24-25 0.5832033403362971 1104 26-27 1.3624282095381446 1104 28-29 1.7773266099179992 1104 30-31 1.403423562911705 1104 32-33 1.1275363274161734 1104 34-35 0.386090024232395 1104 36-37 2.0927956173039703 1104 38-39 1.677086679762759 1104 40-41 1.9993783005905712 1104 42-43 0.041476062547339154 1104 44-45 0.847333790501132 1104 46-47 1.2396511688903757 1104 48-49 -0.3059354707245774 1104 50-51 0.5420673491784243 1104 52-53 0.16434114275843115 1104 54-55 0.2365164242973492 1104 56-57 -0.46386760560118745 1104 58-59 -1.3058484004976947 1104 60-61 0.7597742107518499 1104 62-63 0.6797744429838204 1104 64-65 1.2967647455699947 1104 66-67 0.10657976192602803 1104 68-69 2.272790086459324 1104 70-71 2.708688770125576 1104 72-73 2.236895439043593 1104 74-75 0.9748141507278731 1104 76 -0.8187469854955367 1105 1 1.8037781086803442 1105 2 0.8611540328802292 1105 3 0.515136230370814 1105 4 0.47582871869185084 1105 5 0.011810930587266455 1105 6 0.42905453664035065 1105 7 1.1156413270407484 1105 8 -0.07279026262785493 1105 9 0.6895068475121136 1105 10-11 0.4732614256700032 1105 12-13 -0.9137024773849589 1105 14-15 0.4135512798682157 1105 16-17 0.6466471090439967 1105 18-19 1.0404405181269212 1105 20-21 1.1286561210346164 1105 22-23 0.7221632637404625 1105 24-25 1.5516724043236039 1105 26-27 0.27180155697336517 1105 28-29 1.1069141297487732 1105 30-31 1.847170257782146 1105 32-33 1.5944850318053838 1105 34-35 -0.5203087065978522 1105 36-37 0.516843739990378 1105 38-39 0.7604420472931679 1105 40-41 1.0116072799665616 1105 42-43 0.9804633708498258 1105 44-45 1.1106204113366616 1105 46-47 1.7712482074942884 1105 48-49 0.5588186276360787 1105 50-51 1.15239257392723 1105 52-53 0.7873580650217917 1105 54-55 1.7542980213359485 1105 56-57 1.5675972278202792 1105 58-59 1.389881372109393 1105 60-61 1.813119001867669 1105 62-63 -0.5310267204217851 1105 64-65 0.8573808217201773 1105 66-67 1.4353079480709212 1105 68-69 0.4865658664698955 1105 70-71 1.6849579398770373 1105 72-73 1.4095554073143504 1105 74-75 0.6991663453339001 1105 76 1.8163667109613613 1106 1 -1.3374299874613378 1106 2 0.6393007754482198 1106 3 0.3524227578850443 1106 4 0.18060417726237432 1106 5 0.13942003874665687 1106 6 0.547966617601773 1106 7 -0.6049469082533676 1106 8 0.9125038550192031 1106 9 -0.40568606836391297 1106 10-11 0.5931222732348331 1106 12-13 1.8838939805530543 1106 14-15 -1.1280363229148236 1106 16-17 -0.10785953232223022 1106 18-19 0.4495961158498858 1106 20-21 0.8668756023755506 1106 22-23 1.001179709028257 1106 24-25 -0.072695084607453 1106 26-27 0.6063208485609621 1106 28-29 0.8735175453085517 1106 30-31 1.258855899780876 1106 32-33 0.5832579603316361 1106 34-35 0.6550549872667943 1106 36-37 1.222014517978998 1106 38-39 0.614727309785259 1106 40-41 1.1987514924902811 1106 42-43 1.0381009420073255 1106 44-45 1.3524768313240187 1106 46-47 1.110432268215348 1106 48-49 2.0441127452831367 1106 50-51 0.4998783424281825 1106 52-53 1.1492334603412075 1106 54-55 0.6625048524554984 1106 56-57 0.6634226547652489 1106 58-59 1.1872406383965526 1106 60-61 1.1112689069910076 1106 62-63 1.2228474098754916 1106 64-65 -0.8715091213538173 1106 66-67 0.575962597027285 1106 68-69 2.1837511922130943 1106 70-71 0.6001223927549546 1106 72-73 1.3780879816344012 1106 74-75 0.30621884375262454 1106 76 -0.6377762365402191 1107 1 1.3257920581136524 1107 2 0.032252550752936315 1107 3 0.48411327192849996 1107 4 0.7823529848935351 1107 5 0.6945339762135347 1107 6 0.6836316944433207 1107 7 1.144920606320028 1107 8 0.7087412689036796 1107 9 0.6632063534696826 1107 10-11 1.022375055428789 1107 12-13 -0.24739907721058785 1107 14-15 0.24928217205072656 1107 16-17 1.168770038424995 1107 18-19 1.162897652680833 1107 20-21 1.3491225552107338 1107 22-23 -0.034193899874765066 1107 24-25 0.7251321543962561 1107 26-27 0.2255577325037308 1107 28-29 1.6104014880748423 1107 30-31 1.649952879113151 1107 32-33 1.6571848283761526 1107 34-35 0.671350694738976 1107 36-37 1.8680134580955823 1107 38-39 1.5027771243056591 1107 40-41 0.9625299768570059 1107 42-43 1.5649954836077455 1107 44-45 1.4075907687908895 1107 46-47 0.9351540488194843 1107 48-49 0.9259357447531968 1107 50-51 1.4970961484695238 1107 52-53 0.5909398726358503 1107 54-55 1.422308774024117 1107 56-57 1.5004655800434747 1107 58-59 1.099667771411923 1107 60-61 1.983817926598853 1107 62-63 1.3420842938182602 1107 64-65 1.2689254308454352 1107 66-67 0.735475050657385 1107 68-69 2.0854470056736147 1107 70-71 0.6450349523733934 1107 72-73 2.2008224808394843 1107 74-75 -0.685169603518176 1107 76 1.9727166092467456 1108 1 0.13315824783278174 1108 2 0.4824380303501812 1108 3 -5.184185855426904E-4 1108 4 0.32766300079178734 1108 5 -0.07626623576314984 1108 6 -1.0892882843590144 1108 7 -0.8500449474690583 1108 8 -1.558084380274913 1108 9 -0.6017644997364613 1108 10-11 0.1813575673524781 1108 12-13 1.2368351570236484 1108 14-15 0.7101989712028143 1108 16-17 -0.22060463036144284 1108 18-19 0.5084196452616467 1108 20-21 1.151189327865744 1108 22-23 0.3100032384400251 1108 24-25 1.309657856569018 1108 26-27 -0.8936791514390379 1108 28-29 -1.4206001017502743 1108 30-31 -0.1283990021799113 1108 32-33 0.9411010975865324 1108 34-35 1.003094202953065 1108 36-37 1.5994654983711527 1108 38-39 2.1049233882166334 1108 40-41 0.8163985513138172 1108 42-43 -0.8540559207377711 1108 44-45 0.43581016465734734 1108 46-47 0.9682754054702514 1108 48-49 1.3480343139105884 1108 50-51 0.6322312836046535 1108 52-53 0.5855079701451302 1108 54-55 0.12328916618098162 1108 56-57 0.25165794888290094 1108 58-59 0.1186131874161589 1108 60-61 1.4593081226772782 1108 62-63 1.1885336843852983 1108 64-65 0.26574578060696297 1108 66-67 1.473021420556691 1108 68-69 1.7131629569189784 1108 70-71 -1.7234070190097484 1108 72-73 -0.35230417522834756 1108 74-75 -0.9388791954630662 1108 76 2.068106116400955 1109 1 -2.9465803142587212 1109 2 -0.38096066246027505 1109 3 0.5654946533098766 1109 4 -0.22004941750886786 1109 5 -0.7030636213840609 1109 6 -1.3121641013524794 1109 7 -0.8232475618481416 1109 8 0.04387640403881221 1109 9 1.0328760231393517 1109 10-11 0.06501769807143631 1109 12-13 1.0368351570236456 1109 14-15 0.6366695594381113 1109 16-17 1.042794062449012 1109 18-19 0.7303150700982499 1109 20-21 0.9923657984539744 1109 22-23 0.37601631033544436 1109 24-25 -0.4106035813394797 1109 26-27 0.4769090838550838 1109 28-29 -2.3215804939071347 1109 30-31 0.41016308932335477 1109 32-33 0.47280044399176546 1109 34-35 -0.4939646205763495 1109 36-37 1.5448903349724574 1109 38-39 1.0062305777591192 1109 40-41 0.6575750219020478 1109 42-43 1.8142447328569986 1109 44-45 1.4933265045266282 1109 46-47 0.6375564512218901 1109 48-49 0.27548529430274726 1109 50-51 -0.22887982750646074 1109 52-53 -0.9308318991359172 1109 54-55 -1.2165801148647688 1109 56-57 0.15361873319662323 1109 58-59 -0.10949138774724076 1109 60-61 1.0615957043766215 1109 62-63 1.0767689785029475 1109 64-65 0.8549614668814698 1109 66-67 1.048838414020743 1109 68-69 -0.15088933066272148 1109 70-71 -0.4292893719509223 1109 72-73 -0.3748531948361844 1109 74-75 -1.187572005920579 1109 76 -0.17503113850100505 1110 1 -1.6259453936238017 1110 2 -0.315881297380912 1110 3 -0.22180693399170792 1110 4 -0.2192557667152144 1110 5 0.5770951087746639 1110 6 -0.2153387045270847 1110 7 0.794212755612179 1110 8 1.0819716421340502 1110 9 1.3646220548853876 1110 10-11 -0.06514103208729694 1110 12-13 1.1058827760712617 1110 14-15 0.8211933689619215 1110 16-17 0.6015242211791758 1110 18-19 0.4513468161299983 1110 20-21 1.0209372270254065 1110 22-23 -0.26842813410899424 1110 24-25 -1.6990956448315444 1110 26-27 1.1304805124265087 1110 28-29 0.3308004584738171 1110 30-31 0.6835757877360606 1110 32-33 1.2688321900235096 1110 34-35 1.6000829984712723 1110 36-37 2.383779223861346 1110 38-39 1.5534527999813434 1110 40-41 -1.2662345019074692 1110 42-43 0.5027367963490619 1110 44-45 1.2961042823044053 1110 46-47 0.6331913718568103 1110 48-49 2.338580532397984 1110 50-51 0.05485033122370453 1110 52-53 0.5235331802291654 1110 54-55 -0.15507217835683207 1110 56-57 1.0107615903394844 1110 58-59 -0.9146501179059712 1110 60-61 0.5076274504083713 1110 62-63 -0.8898976881637211 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-2.7922237400678185 2116 62-63 -0.37427268816372106 2116 64-65 -2.2419135331185274 2116 66-67 -1.231022697090367 2116 68-69 -2.6606115528849443 2116 70-71 0.19050229471574198 2116 72-73 -0.7300615281695215 2116 74-75 -1.2559747836983561 2116 76 -3.5666978051676708 2117 1 0.09786413018572304 2117 2 1.0745948930952807 2117 3 -0.11228312446790056 2117 4 0.3355061380466893 2117 5 0.14138082306038058 2117 6 0.2656136764252963 2117 7 0.4656413270407498 2117 8 0.9105430707054794 2117 9 -0.31156842130508977 2117 10-11 -0.11276007970634794 2117 12-13 -1.0131648429763516 2117 14-15 0.7700028927714442 2117 16-17 -0.16276149310654375 2117 18-19 -0.6307960410128644 2117 20-21 -0.4409675348793556 2117 22-23 0.1815718658910015 2117 24-25 -1.9883813591172554 2117 26-27 -1.223090916144919 2117 28-29 -2.304913827240469 2117 30-31 -1.0009480217877496 2117 32-33 -3.1716440004526802 2117 34-35 -0.11063128724300952 2117 36-37 0.4504458905280089 2117 38-39 0.7784527999813449 2117 40-41 -0.27575831143128227 2117 42-43 -0.9413108226985614 2117 44-45 -0.29556238436226323 2117 46-47 0.08200089566633295 2117 48-49 0.3754852943027416 2117 50-51 -0.051102049728683596 2117 52-53 -0.1919430102470301 2117 54-55 0.20564210735745547 2117 56-57 -0.49638126680337535 2117 58-59 0.4127308344749778 2117 60-61 0.6338179265988444 2117 62-63 1.0601023118362818 2117 64-65 -0.11170519978519877 2117 66-67 0.4543939695763015 2117 68-69 1.37688844711505 2117 70-71 0.0873772947157434 2117 72-73 2.0751468051638113 2117 74-75 1.3346502163016467 2117 76 1.2916355281656635 2118 1 -1.1918794595578674 2118 2 -1.2920717735713865 2118 3 -2.9892062013909744 2118 4 -0.910647708107156 2118 5 0.37471415639371486 2118 6 -0.2446427338311139 2118 7 -0.44205098065155823 2118 8 -1.0920210318586214 2118 9 -5.085927395664065 2118 10-11 -0.29224725919352323 2118 12-13 -1.9490622788737895 2118 14-15 -1.8376894149208596 2118 16-17 -5.215325595670642 2118 18-19 -1.1974627076795272 2118 20-21 -1.830711124622944 2118 22-23 0.5649051992243344 2118 24-25 0.9167468460109447 2118 26-27 -2.1410396340936373 2118 28-29 -0.3651702374968835 2118 30-31 -5.026589047428779 2118 32-33 -2.88318246199114 2118 34-35 -0.6760159026276256 2118 36-37 -4.770066929984807 2118 38-39 -1.2125728410442989 2118 40-41 0.8344980988251294 2118 42-43 1.0086891773014415 2118 44-45 -3.655818794618675 2118 46-47 -1.051332437667 2118 48-49 0.0203570891745386 2118 50-51 -2.2331533317799597 2118 52-53 -2.189378907682922 2118 54-55 -1.4879476362322919 2118 56-57 -0.6861248565469609 2118 58-59 -2.4206024988583508 2118 60-61 -2.279002586221665 2118 62-63 -0.9860515343175607 2118 64-65 -0.31042314850314057 2118 66-67 -1.8007342355519 2118 68-69 -2.030803860577251 2118 70-71 -1.8972380898996448 2118 72-73 -0.5197249897079814 2118 74-75 -0.9615036298522028 2118 76 0.9044560409861759 2119 1 1.0645307968523916 2119 2 0.41626155976194923 2119 3 -0.9539497911345656 2119 4 -0.9811605286199736 2119 5 -1.7502858436062851 2119 6 -0.9177196569080337 2119 7 -1.5093586729592516 2119 8 0.010543070705480773 2119 9 0.05509824536157737 2119 10-11 -0.029426746373012236 2119 12-13 -0.6381648429763516 2119 14-15 0.532502892771447 2119 16-17 -0.8210948264398752 2119 18-19 -1.3432960410128558 2119 20-21 -0.8243008682126955 2119 22-23 -0.10176146744233705 2119 24-25 0.544951974216076 2119 26-27 0.8477424171884138 2119 28-29 0.7950861727595253 2119 30-31 2.207385311545579 2119 32-33 2.3491893328806555 2119 34-35 0.21020204609031623 2119 36-37 -0.1995541094719826 2119 38-39 -0.5507138666853209 2119 40-41 1.4659083552353778 2119 42-43 1.4670225106347772 2119 44-45 0.6919376156377339 2119 46-47 1.011167562333 2119 48-49 -0.1286813723639213 2119 50-51 0.7988979502713249 2119 52-53 0.9580569897529756 2119 54-55 1.2556421073574597 2119 56-57 -1.4922146001367125 2119 58-59 -0.17060249885835077 2119 60-61 -0.3078487400678185 2119 62-63 1.1309356451696146 2119 64-65 0.24246146688147263 2119 66-67 0.6335606362429616 2119 68-69 1.9852217804483843 2119 70-71 1.7582106280490706 2119 72-73 1.5459801384971428 2119 74-75 0.051316882968308164 2119 76 0.6416355281656649 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 4.0 22 3.0 23 13.0 24 17.0 25 47.0 26 98.0 27 177.0 28 310.0 29 520.0 30 897.0 31 1322.0 32 2108.0 33 2970.0 34 4121.0 35 5228.0 36 6122.0 37 4214.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.3932983103791 26.544086326849353 11.969331250887407 26.09328411188414 2 18.245066023001563 21.042169530029817 36.997728240806474 23.715036206162146 3 17.865256282834018 26.824506602300158 29.16370864688343 26.146528467982392 4 13.183302569927587 18.614226891949453 39.645747550759616 28.556722987363344 5 12.672156751384353 38.14425670878887 36.117421553315346 13.066164986511431 6 27.715462161010933 39.34402953286952 18.3018600028397 14.638648303279853 7 26.55118557432912 30.97046713048417 23.87476927445691 18.603578020729802 8 22.68564532159591 35.730512565668036 20.86823796677552 20.71560414596053 9 22.987363339486013 15.998154195655259 23.45236404941076 37.56211841544796 10-11 24.7195797245492 25.342538690898763 27.774030952719013 22.163850631833025 12-13 23.872994462586966 23.681314780633254 26.840479909129634 25.60521084765015 14-15 21.100738321737897 26.28141417009797 24.07709782763027 28.54074968053386 16-17 21.705949169388045 28.4786312650859 26.224620190259834 23.590799375266222 18-19 21.358086042879453 28.354394434189977 26.994888541814564 23.292630981116 20-21 21.020871787590515 28.494604571915378 27.11557574897061 23.368947891523497 22-23 22.089308533295473 28.223058355814285 27.68529035922192 22.002342751668323 24-25 21.386483032798523 29.151284963793838 27.12622462019026 22.336007383217378 26-27 20.40678688059066 29.497373278432487 27.37647309385205 22.719366747124806 28-29 21.892304415731935 27.254011074826067 28.70935680817833 22.144327701263666 30-31 21.537342041743575 27.763382081499362 28.652562828340194 22.04671304841687 32-33 21.711273604997867 29.160159023143546 27.159946045719153 21.968621326139427 34-35 21.677552179468975 28.311798949311374 28.27630271191254 21.734346159307112 36-37 21.858582990203036 27.644469686213263 27.40309527190118 23.09385205168252 38-39 21.13623455913673 28.560272611103223 27.605423825074542 22.698069004685504 40-41 20.921482322873775 28.029603861990626 28.42716172085759 22.621752094278005 42-43 21.574613091012353 27.70481328979128 28.595768848502058 22.124804770694308 44-45 20.772398125798667 28.063325287519525 27.82195087320744 23.34232571347437 46-47 21.49474655686497 27.827275308817267 27.674641488002273 23.00333664631549 48-49 20.893085332954705 27.80065313076814 28.840692886554027 22.46556864972313 50-51 20.56474513701548 27.816626437597613 28.24613091012353 23.372497515263383 52-53 20.61089024563396 27.271759193525487 29.28617066590941 22.831179894931136 54-55 20.12281698139997 27.89116853613517 29.200979696152206 22.78503478631265 56-57 20.76352406644896 27.823725685077385 28.57447110606276 22.838279142410904 58-59 20.137015476359508 28.482180888825788 28.14141700979696 23.23938662501775 60-61 20.374840266931706 28.542524492403803 28.13076813857731 22.951867102087178 62-63 19.295754650007098 28.911685361351697 28.075748970609116 23.71681101803209 64-65 20.25947749538549 29.673079653556723 26.87065171091864 23.196791140139144 66-67 19.789152349850916 29.128212409484593 27.534431350276872 23.54820389038762 68-69 19.636518529035925 28.95073122249042 27.46698849921908 23.94576174925458 70-71 19.829973022859576 29.108689478915235 27.266434757915658 23.794902740309524 72-73 19.922263240096548 27.857447110606277 27.477637370438735 24.74265227885844 74-75 20.035851199772825 28.505253443135025 27.000212977424393 24.458682379667756 76 20.144114723839273 28.49637938378532 26.994888541814564 24.36461735056084 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 0.0 16 1.5 17 3.0 18 3.5 19 7.0 20 11.5 21 13.0 22 18.0 23 24.0 24 29.5 25 34.0 26 64.0 27 112.0 28 152.5 29 175.0 30 212.0 31 333.5 32 486.0 33 554.0 34 619.5 35 785.0 36 948.5 37 1012.0 38 1154.5 39 1491.5 40 1830.0 41 1984.0 42 1994.0 43 2094.0 44 2185.5 45 2158.0 46 2139.0 47 2102.0 48 1989.0 49 1716.0 50 1519.0 51 1395.5 52 1087.5 53 850.0 54 797.0 55 733.0 56 556.0 57 378.0 58 313.0 59 245.0 60 153.0 61 106.5 62 84.0 63 61.0 64 37.5 65 25.0 66 14.5 67 16.0 68 10.5 69 3.0 70 1.0 71 1.0 72 1.0 73 0.5 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 28172.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.8780349282976 #Duplication Level Percentage of deduplicated Percentage of total 1 75.44879756125098 47.440721283543944 2 12.18245455571864 15.320176061337499 3 5.199277407700125 9.80761039329831 4 2.8226261713898615 7.099247479767144 5 1.5355086372360844 4.827488286241658 6 0.9427571412442137 3.5567229873633393 7 0.5983967483346506 2.633820814993611 8 0.4121034210229197 2.0729802640920063 9 0.2709721124534267 1.5334374556297032 >10 0.5758157389635317 4.831037909981542 >50 0.005645252342779722 0.2555729092716172 >100 0.005645252342779722 0.6211841544796252 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT 175 0.6211841544796252 RNA PCR Primer, Index 47 (95% over 21bp) ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 72 0.2555729092716172 No Hit CTTTACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC 30 0.10648871219650717 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.010648871219650717 0.0 21 0.0 0.0 0.0 0.010648871219650717 0.0 22 0.0 0.0 0.0 0.010648871219650717 0.0 23 0.0 0.0 0.0 0.010648871219650717 0.0 24 0.0 0.0 0.0 0.010648871219650717 0.0 25 0.0 0.0 0.0 0.010648871219650717 0.0 26 0.0 0.0 0.0 0.01419849495953429 0.0 27 0.0 0.0 0.0 0.01419849495953429 0.0 28 0.0 0.0 0.0 0.01774811869941786 0.0 29 0.0 0.0 0.0 0.01774811869941786 0.0 30 0.0 0.0 0.0 0.01774811869941786 0.0 31 0.0 0.0 0.0 0.021297742439301433 0.0 32 0.0 0.0 0.0 0.03194661365895215 0.0 33 0.0 0.0 0.0 0.039045861138719294 0.0 34 0.0 0.0 0.0 0.04259548487860287 0.0 35 0.0 0.0 0.0 0.04969473235837001 0.0 36 0.0 0.0 0.0 0.07454209853755502 0.0 37 0.0 0.0 0.0 0.13488570211557574 0.0 38 0.0 0.0 0.0 0.1668323157745279 0.0 39 0.0 0.0 0.0 0.2307255430924322 0.0 40 0.0 0.0 0.0 0.3194661365895215 0.0 41 0.0 0.0 0.0 0.45080221496521367 0.0 42 0.0 0.0 0.0 0.6602300156183445 0.0 43 0.0 0.0 0.0 0.8661081925315917 0.0 44 0.0 0.0 0.0 1.0329405083061196 0.0 45 0.0 0.0 0.0 1.2423683089592503 0.0 46 0.0 0.0 0.0 1.4801930995314496 0.0 47 0.0 0.0 0.0 1.7499645037626013 0.0 48 0.0 0.0 0.0 1.9984381655544512 0.0 49 0.0 0.0 0.0 2.2788584410052533 0.0 50 0.0 0.0 0.0 2.4776373704387336 0.0 51 0.0 0.0 0.0 2.7367599034502343 0.0 52 0.0 0.0 0.0 3.137867386057078 0.0 53 0.0 0.0 0.0 3.4715320176061337 0.0 54 0.0 0.0 0.0 3.8158455203748405 0.0 55 0.0 0.0 0.0 4.2169530029816835 0.0 56 0.0 0.0 0.0 4.518671020871787 0.0 57 0.0 0.0 0.0 4.841686781201193 0.0 58 0.0 0.0 0.0 5.090160442993043 0.0 59 0.0 0.0 0.0 5.377679965923612 0.0 60 0.0 0.0 0.0 5.679397983813716 0.0 61 0.0 0.0 0.0 5.931421269345449 0.0 62 0.0 0.0 0.0 6.364475365611245 0.0 63 0.0 0.0 0.0 6.751384353258555 0.0 64 0.0 0.0 0.0 7.18443844952435 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCTCAT 15 0.0022023655 70.0 35 ACGTCTC 20 0.0068821483 52.5 58 TAAAAGG 20 0.0068821483 52.5 4 TACGTCT 20 0.0068821483 52.5 57 TCTCGAG 20 0.0068821483 52.5 61 GTCTCGA 20 0.0068821483 52.5 60 CGAGTGA 20 0.0068821483 52.5 64 TTCAGCC 20 0.0068821483 52.5 44 TTTGTAG 20 0.0068821483 52.5 35 CTCGAGT 20 0.0068821483 52.5 62 CTGCTTG 55 2.9204317E-4 31.818184 51 TTTTTTC 45 0.004238128 31.111113 24 AGGAGTA 45 0.004238128 31.111113 21 CTTCTGC 60 4.863305E-4 29.166666 48 TTAGGGT 50 0.0070933746 27.999998 4 TCTGCTT 65 7.767088E-4 26.923077 50 CTTGAAA 65 7.767088E-4 26.923077 54 TCTTCTG 65 7.767088E-4 26.923077 47 TGCTTGA 70 0.0011970759 25.0 52 GCTTGAA 70 0.0011970759 25.0 53 >>END_MODULE