FastQCFastQC Report
Wed 25 May 2016
SRR1780589_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780589_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences797091
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCCG357.312199E-637.695611
CTTACGC250.002370412735.1450429
AATTCAG602.8964132E-729.38709318
TTCAGAA804.2200554E-927.55560320
AAATTCA655.8521255E-727.11630617
TCCGTAT1101.9826984E-1024.03188113
GCGTGTA551.6024032E-423.97907631
CAAGTCG752.0302068E-623.50967218
ATTCAGA752.0302068E-623.50967219
TACTCGC1153.4197E-1023.00293520
TATATGT1205.9117156E-1022.01952738
ATCGGTT1004.6155037E-822.0015422
GTACATG1105.2768883E-921.9891031
AATTATC1105.2805262E-921.98772230
CCGCGTG500.00259276821.98082231
CGGTATG904.1200474E-721.97668343
ACCGCAC602.903322E-421.9642758
GGTCCAA1351.1277734E-1021.19332711
ACTCGCA1259.695214E-1021.16270321
GCGCTAT1259.731593E-1021.15870719
Produced by FastQC (version 0.11.5)