FastQCFastQC Report
Wed 25 May 2016
SRR1780589_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780589_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences797091
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT99191.2443999493156992No Hit
ATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCTT41820.5246577868775334TruSeq Adapter, Index 10 (95% over 21bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC14940.18743154796629244No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC13000.16309304709249006No Hit
TCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG8950.11228329011367585No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT4950.036.88546844
CCCGTGT301.3014465E-436.6632719
ATATCAG250.00235338335.196745
CGCTTGC250.00235338335.1967424
GCCGTCT5600.032.60412243
ATGATTC2450.030.52778240
CGTGCGA752.1573214E-929.3416610
CCGCACG300.005737651629.3398199
CGGAGAT901.6370905E-1129.33061630
TCTGACG300.005746455429.33061426
TCCCTCG300.005746455429.33061425
AAAACCG300.005746455429.33061426
CGGGCCT300.005746455429.33061439
ATTGGCG2750.028.7973321
CATGCGG953.274181E-1127.786926
GCGTGAA2800.027.49745425
GACGCAG407.033824E-427.49745229
CGCCATT3100.026.96524417
TGAAGGT2900.026.54926528
CCGAAGT1006.002665E-1126.40749414
Produced by FastQC (version 0.11.5)