FastQCFastQC Report
Wed 25 May 2016
SRR1780588_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780588_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2860
Sequences flagged as poor quality0
Sequence length76
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTG50.17482517482517482No Hit
CAAAAGGAAACATATGTTCACAGTGGGAAGGATTATTACTCTGTCTCTTA40.13986013986013987No Hit
CTGTAATGCCAGCACTTTGGAAAGACGAGGAGGGCAGATCACTTCTGTCT30.1048951048951049No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATC30.1048951048951049No Hit
CCATGAATCCAACAGCCCAGTGGATGAAATTTTGCTTATGTGTATTTCAC30.1048951048951049No Hit
GGTTCTTACCTCCTGGTATTAATGCCGTTGTATAATCTCCCCATCTTGAG30.1048951048951049No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers