##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780588_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2860 Sequences flagged as poor quality 0 Sequence length 76 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.597202797202797 23.0 12.0 32.0 12.0 34.0 2 20.246503496503497 21.0 11.0 31.0 11.0 34.0 3 19.1506993006993 11.0 11.0 27.0 11.0 34.0 4 20.216433566433565 21.0 11.0 31.0 11.0 34.0 5 20.081818181818182 21.0 11.0 31.0 11.0 34.0 6 21.71818181818182 21.0 11.0 33.0 11.0 37.0 7 22.774825174825175 24.0 11.0 34.0 11.0 37.0 8 23.15 26.0 11.0 34.0 11.0 37.0 9 22.745804195804197 23.0 12.0 34.0 12.0 37.0 10-11 21.397202797202798 21.0 11.0 32.5 11.0 37.0 12-13 21.31451048951049 21.0 11.0 31.0 11.0 36.5 14-15 21.080944055944055 21.0 11.0 31.0 11.0 36.5 16-17 20.482867132867135 16.5 11.0 29.5 11.0 36.0 18-19 21.001573426573426 21.0 11.0 31.0 11.0 36.0 20-21 21.208916083916083 21.5 11.0 31.0 11.0 36.0 22-23 20.470454545454544 16.0 11.0 29.5 11.0 36.5 24-25 20.56590909090909 21.0 11.0 29.5 10.5 36.0 26-27 20.079195804195805 21.0 11.0 28.0 10.5 36.0 28-29 19.65909090909091 11.0 11.0 27.0 11.0 35.5 30-31 19.627797202797204 11.0 11.0 27.0 11.0 35.0 32-33 19.950874125874126 16.0 11.0 27.0 11.0 35.5 34-35 20.193706293706292 16.0 11.0 27.0 11.0 35.5 36-37 20.00821678321678 16.0 11.0 27.0 11.0 35.5 38-39 19.538286713286713 11.0 11.0 27.0 11.0 34.0 40-41 19.7763986013986 16.0 11.0 27.0 11.0 34.5 42-43 19.85979020979021 16.0 11.0 27.0 11.0 34.5 44-45 19.28181818181818 11.0 11.0 26.0 11.0 34.0 46-47 20.082342657342657 16.0 11.0 27.0 11.0 34.5 48-49 20.146153846153847 21.0 11.0 27.0 11.0 34.5 50-51 20.199125874125873 21.0 11.0 27.0 11.0 34.0 52-53 20.23111888111888 21.0 11.0 27.0 11.0 34.0 54-55 20.265034965034964 21.0 11.0 27.0 11.0 34.0 56-57 19.55839160839161 16.0 11.0 26.5 11.0 34.0 58-59 19.54300699300699 16.0 11.0 26.5 11.0 34.0 60-61 19.917657342657343 19.5 11.0 26.5 11.0 34.0 62-63 19.282517482517484 15.0 11.0 26.0 11.0 34.0 64-65 19.615384615384613 15.5 11.0 26.5 11.0 34.0 66-67 19.98513986013986 19.0 11.0 27.0 11.0 34.0 68-69 19.647377622377626 15.0 11.0 27.0 10.5 34.0 70-71 19.39423076923077 19.0 11.0 26.5 11.0 34.0 72-73 19.736363636363635 19.0 11.0 26.5 11.0 34.0 74-75 19.731293706293705 19.0 11.0 27.0 11.0 33.5 76 19.72132867132867 19.0 11.0 26.0 11.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5810359231411866 1101 2 -0.4264154704944296 1101 3 -7.13555764411028 1101 4 -2.1138480671375426 1101 5 -4.938783511809831 1101 6 -1.9438036378825885 1101 7 -1.134036796536801 1101 8 3.000388281309334 1101 9 0.20973646236803845 1101 10-11 -4.3574162679425825 1101 12-13 0.011248765854031006 1101 14-15 -0.5548886420596943 1101 16-17 0.23153385357332468 1101 18-19 -2.0695450748082322 1101 20-21 -2.3739846776030973 1101 22-23 -0.983209918736236 1101 24-25 0.528398173463966 1101 26-27 -4.24058156755525 1101 28-29 -4.6002463545226675 1101 30-31 -2.994920539986328 1101 32-33 -6.43783416875522 1101 34-35 -2.8062670881749803 1101 36-37 -0.1288828130933375 1101 38-39 -2.3924645895040655 1101 40-41 -0.581764069264068 1101 42-43 -0.7900095883648461 1101 44-45 0.2849073441178689 1101 46-47 -1.2318860598465875 1101 48-49 -1.683939963545221 1101 50-51 0.5835977253740481 1101 52-53 0.0531356801093672 1101 54-55 -2.852069093187513 1101 56-57 -1.9837287347155765 1101 58-59 -3.0593780853649264 1101 60-61 -1.1064336978810694 1101 62-63 -0.12246857674489675 1101 64-65 -2.8265616693248248 1101 66-67 -0.7589409128882778 1101 68-69 -3.2671669704564437 1101 70-71 3.8152307852965777 1101 72-73 -2.1170032087795256 1101 74-75 -3.61389031290347 1101 76 -1.1107532087795278 1102 1 -3.0856307435254813 1102 2 2.873584529505571 1102 3 -0.0688909774436155 1102 4 -2.9249591782486526 1102 5 -1.8165612895876109 1102 6 -4.110470304549256 1102 7 0.24374098124097898 1102 8 -3.4329450520239995 1102 9 -0.5902635376319623 1102 10-11 0.4036948431685268 1102 12-13 0.33347098807625386 1102 14-15 -0.5937775309485822 1102 16-17 2.4648671869066554 1102 18-19 1.20267714741399 1102 20-21 -0.951762455380873 1102 22-23 1.4223456368193226 1102 24-25 -2.5549351598693697 1102 26-27 2.609418432444752 1102 28-29 1.4386425343662204 1102 30-31 -1.317142762208551 1102 32-33 -0.31561194653300007 1102 34-35 -1.2562670881749796 1102 36-37 -1.8066605908711146 1102 38-39 -3.8535757006151776 1102 40-41 0.546013708513712 1102 42-43 -0.7900095883648461 1102 44-45 1.5737962330067568 1102 46-47 -0.8318860598465889 1102 48-49 4.743837814232556 1102 50-51 -4.7275133857370655 1102 52-53 -2.1857532087795235 1102 54-55 0.209042017923597 1102 56-57 -2.1615065124933537 1102 58-59 2.096177470190632 1102 60-61 2.810232968785595 1102 62-63 2.827531423255106 1102 64-65 2.5623272195640645 1102 66-67 -2.2422742462216085 1102 68-69 -0.30050030378977866 1102 70-71 -0.8069914369256423 1102 72-73 0.5552190134426986 1102 74-75 -0.8583347573479152 1102 76 -1.810753208779527 1103 1 -3.903812561707298 1103 2 -0.6082336523126131 1103 3 0.3492908407382025 1103 4 -0.40677736006683674 1103 5 -1.8983794714057929 1103 6 0.662256968178017 1103 7 2.6437409812409776 1103 8 -1.6965814156603614 1103 9 -1.6993544467228716 1103 10-11 -1.3826687931951085 1103 12-13 -1.1301653755601109 1103 14-15 1.192586105415053 1103 16-17 0.4739580959975669 1103 18-19 -1.801868307131464 1103 20-21 -1.4244897281081492 1103 22-23 -3.245836181362499 1103 24-25 -0.8958442507784596 1103 26-27 -0.14967247664615613 1103 28-29 -4.256812011088323 1103 30-31 -0.8989609440267294 1103 32-33 -1.0337937647148188 1103 34-35 2.1028238209159262 1103 36-37 2.0024303182197905 1103 38-39 2.2236970266575504 1103 40-41 0.6051046176046171 1103 42-43 -2.4263732247284793 1103 44-45 -0.41711285790233177 1103 46-47 0.08629575833523262 1103 48-49 1.9574741778689209 1103 50-51 1.4724866142629338 1103 52-53 -1.9266622996886156 1103 54-55 2.1681329270145078 1103 56-57 3.7839480329611916 1103 58-59 -1.9129134389002793 1103 60-61 0.10568751424014167 1103 62-63 0.2866223323460133 1103 64-65 -0.8922182349813887 1103 66-67 -1.2286378825852466 1103 68-69 -2.327773031062506 1103 70-71 -1.861536891471097 1103 72-73 -0.12205371383003083 1103 74-75 1.2143925153793589 1103 76 0.25288315485683555 1104 1 -0.6689640768588134 1104 2 -0.09308213716109748 1104 3 -2.968890977443614 1104 4 4.358374155084679 1104 5 2.9501053770790584 1104 6 -1.6104703045492563 1104 7 -2.9395923520923546 1104 8 2.417054947976002 1104 9 1.7930697957013706 1104 10-11 1.2536948431685282 1104 12-13 -2.2665290119237476 1104 14-15 4.264555802384752 1104 16-17 4.356533853573321 1104 18-19 0.9026771474139892 1104 20-21 0.764904211285792 1104 22-23 0.9890123034859855 1104 24-25 0.4867315067972946 1104 26-27 -0.6572482342219139 1104 28-29 1.371975867699554 1104 30-31 4.366190571124783 1104 32-33 2.2843880534670014 1104 34-35 -0.014600421508315975 1104 36-37 2.9266727424622196 1104 38-39 6.0797576327181595 1104 40-41 4.446013708513711 1104 42-43 3.1266570783018217 1104 44-45 0.007129566340090321 1104 46-47 -0.9818860598465875 1104 48-49 2.385504480899222 1104 50-51 3.0558199475962695 1104 52-53 1.1225801245538136 1104 54-55 -1.8242913154097344 1104 56-57 2.905160154173309 1104 58-59 1.537844136857295 1104 60-61 -1.6064336978810694 1104 62-63 1.7525314232551032 1104 64-65 -0.1876727804359355 1104 66-67 -0.8839409128882743 1104 68-69 2.357833029543553 1104 70-71 3.0513418964076884 1104 72-73 -0.3947809865573042 1104 74-75 2.5666652426520855 1104 76 4.722580124553804 1105 1 -0.08563074352548128 1105 2 2.8235845295055704 1105 3 -2.635557644110282 1105 4 1.9417074884180145 1105 5 -1.9665612895876095 1105 6 1.0561963621174115 1105 7 2.0604076479076454 1105 8 -3.082945052023998 1105 9 0.7930697957013706 1105 10-11 0.3786948431685282 1105 12-13 -1.9331956785904119 1105 14-15 0.18122246905141637 1105 16-17 1.9815338535733211 1105 18-19 -0.9306561859193465 1105 20-21 0.18157087795245985 1105 22-23 -1.8443210298473467 1105 24-25 2.6117315067972946 1105 26-27 2.8010850991114147 1105 28-29 -0.3780241323004425 1105 30-31 0.0745239044581183 1105 32-33 0.8260547201336657 1105 34-35 0.027066245158351876 1105 36-37 1.8016727424622196 1105 38-39 -1.3785757006151762 1105 40-41 1.6126803751803784 1105 42-43 -0.6233429216981818 1105 44-45 1.3821295663400939 1105 46-47 -1.0652193931799232 1105 48-49 2.010504480899222 1105 50-51 0.8891532809296017 1105 52-53 -2.419086542112858 1105 54-55 -1.6576246487430701 1105 56-57 -0.1365065124933551 1105 58-59 1.8711774701906307 1105 60-61 3.268566302118927 1105 62-63 1.1691980899217675 1105 64-65 3.7706605528974 1105 66-67 0.07439242044505434 1105 68-69 -1.4755003037897794 1105 70-71 -1.6986581035923116 1105 72-73 -0.3114476532239685 1105 74-75 2.2333319093187534 1105 76 -1.44408654211286 1106 1 -1.3583580162527547 1106 2 -2.608233652312613 1106 3 1.7129272043745694 1106 4 -0.6795046327941066 1106 5 -0.534743107769426 1106 6 5.298620604541654 1106 7 -1.6289862914862958 1106 8 0.576145857066912 1106 9 0.9370091896407651 1106 10-11 -3.4281233386496517 1106 12-13 -1.357438102832841 1106 14-15 -0.17105025822131026 1106 16-17 -1.2987691767297065 1106 18-19 -2.620050125313284 1106 20-21 1.2573284537100307 1106 22-23 -1.4731089086352256 1106 24-25 -1.1231169780511898 1106 26-27 -2.4223997493734295 1106 28-29 -2.802266556542868 1106 30-31 -0.5353245803903661 1106 32-33 0.920751689830638 1106 34-35 -2.669903451811347 1106 36-37 -0.8612060454165693 1106 38-39 0.7236970266575504 1106 40-41 0.5596500721500739 1106 42-43 -0.15364595200120945 1106 44-45 -1.4171128579023353 1106 46-47 -1.3682496962102242 1106 48-49 2.866565086959831 1106 50-51 1.290668432444754 1106 52-53 0.982428609402298 1106 54-55 0.3499511088326912 1106 56-57 -0.6705974215842652 1106 58-59 -0.4583679843548225 1106 60-61 -0.7579488493962252 1106 62-63 3.2411677868914666 1106 64-65 -2.3467636895268456 1106 66-67 0.2713621174147569 1106 68-69 -1.4641366674261462 1106 70-71 -1.270627800562007 1106 72-73 -2.8493264411027575 1106 74-75 -0.5583347573479145 1106 76 4.798337700311382 1107 1 4.914369256474519 1107 2 3.9069178628389025 1107 3 1.9477756892230538 1107 4 3.6917074884180145 1107 5 2.1167720437457227 1107 6 3.972863028784076 1107 7 4.643740981240978 1107 8 -0.4996117186906659 1107 9 7.2097364623680384 1107 10-11 2.462028176501864 1107 12-13 4.233470988076252 1107 14-15 0.6812224690514164 1107 16-17 4.231533853573321 1107 18-19 3.736010480747325 1107 20-21 4.1399042112857884 1107 22-23 3.322345636819321 1107 24-25 3.361731506797298 1107 26-27 3.8010850991114147 1107 28-29 1.3719758676995575 1107 30-31 1.6995239044581147 1107 32-33 3.7843880534670014 1107 34-35 6.44373291182502 1107 36-37 5.801672742462216 1107 38-39 2.663090966051488 1107 40-41 3.1960137085137106 1107 42-43 2.4183237449684896 1107 44-45 0.7154628996734225 1107 46-47 0.4764472734867482 1107 48-49 5.885504480899222 1107 50-51 -0.6108467190704019 1107 52-53 1.539246791220478 1107 54-55 3.1340420179235977 1107 56-57 5.405160154173309 1107 58-59 1.412844136857295 1107 60-61 2.7685663021189306 1107 62-63 2.1275314232551032 1107 64-65 3.1039938862307324 1107 66-67 1.28272575377839 1107 68-69 3.1078330295435563 1107 70-71 2.2596752297410205 1107 72-73 5.230219013442696 1107 74-75 2.98333190931875 1107 76 3.05591345788714 1108 1 -0.885630743525482 1108 2 -3.326415470494428 1108 3 0.33110902255638663 1108 4 -1.9249591782486526 1108 5 -3.116561289587608 1108 6 -0.41047030454925704 1108 7 -2.156259018759023 1108 8 -1.4329450520239995 1108 9 -0.9902635376319608 1108 10-11 0.6536948431685268 1108 12-13 -1.4665290119237504 1108 14-15 1.106222469051417 1108 16-17 0.864867186906654 1108 18-19 -2.6973228525860122 1108 20-21 -2.901762455380876 1108 22-23 -1.0776543631806774 1108 24-25 -2.7549351598693725 1108 26-27 -1.3905815675552482 1108 28-29 0.2886425343662218 1108 30-31 -0.6671427622085488 1108 32-33 -1.165611946532998 1108 34-35 0.04373291182502115 1108 36-37 -3.156660590871116 1108 38-39 -4.703575700615177 1108 40-41 -3.203986291486288 1108 42-43 -5.090009588364847 1108 44-45 -1.4262037669932432 1108 46-47 -2.9818860598465875 1108 48-49 1.0438378142325568 1108 50-51 -1.8775133857370676 1108 52-53 -4.735753208779521 1108 54-55 0.3090420179235984 1108 56-57 -2.6615065124933537 1108 58-59 -3.6538225298093696 1108 60-61 -1.939767031214405 1108 62-63 -3.4224685767448975 1108 64-65 -4.1876727804359355 1108 66-67 -2.59227424622161 1108 68-69 -0.8505003037897794 1108 70-71 -0.5069914369256452 1108 72-73 -0.7947809865573028 1108 74-75 -2.5583347573479145 1108 76 4.5892467912204715 1109 1 0.1870965292017921 1109 2 -3.0627791068580663 1109 3 -1.3779818865345241 1109 4 -4.406777360066835 1109 5 -0.8983794714057929 1109 6 1.2077115136325602 1109 7 0.7346500721500675 1109 8 -0.6056723247512714 1109 9 -1.6084453558137781 1109 10-11 -1.3826687931951085 1109 12-13 0.9607437153489791 1109 14-15 1.4653133781423264 1109 16-17 2.564867186906657 1109 18-19 2.9254044201412626 1109 20-21 -4.015398819017239 1109 22-23 -3.200381635907952 1109 24-25 -3.62311697805119 1109 26-27 -1.8769452039188863 1109 28-29 -0.25681201108832497 1109 30-31 0.9192208741550871 1109 32-33 -0.03379376471481521 1109 34-35 4.19373291182502 1109 36-37 4.0024303182197905 1109 38-39 -0.41266660970608626 1109 40-41 -2.6676226551226527 1109 42-43 -0.653645952001213 1109 44-45 -2.0080219488114253 1109 46-47 -1.9137042416647674 1109 48-49 -0.31525309485834896 1109 50-51 1.5633957051720273 1109 52-53 -0.6993895724158854 1109 54-55 -2.5136852548036757 1109 56-57 -1.4887792397660817 1109 58-59 -0.27654980253664263 1109 60-61 -2.8943124857598583 1109 62-63 -0.44065039492671687 1109 64-65 -1.346763689526842 1109 66-67 -0.5468197007670632 1109 68-69 -1.4186821219715995 1109 70-71 0.09300856307435623 1109 72-73 -0.21296280473912432 1109 74-75 -2.1037893028024612 1109 76 0.5256104275841089 1110 1 0.24770258980785087 1110 2 -2.204193248272208 1110 3 -3.6911131996658355 1110 4 -3.002736956026432 1110 5 -3.938783511809831 1110 6 0.6117519176729651 1110 7 -2.578481240981244 1110 8 -2.999611718690666 1110 9 -3.4569302042986294 1110 10-11 0.9759170653907496 1110 12-13 -2.4331956785904154 1110 14-15 -0.27711086428191933 1110 16-17 -2.379577257537786 1110 18-19 -1.1806561859193465 1110 20-21 0.07045976684134558 1110 22-23 -1.8165432520695646 1110 24-25 -1.1382684932027054 1110 26-27 1.4816406546669718 1110 28-29 -1.7113574656337782 1110 30-31 -2.939364984430771 1110 32-33 -3.2156119465329986 1110 34-35 -5.19515597706387 1110 36-37 -1.1288828130933375 1110 38-39 -2.6146868117262905 1110 40-41 -1.3595418470418466 1110 42-43 -1.5677873661426247 1110 44-45 1.1737962330067582 1110 46-47 0.5458917179311911 1110 48-49 3.816060036454779 1110 50-51 -1.916402274625952 1110 52-53 -3.0024198754461864 1110 54-55 -0.852069093187513 1110 56-57 -2.372617623604466 1110 58-59 -4.11493364092048 1110 60-61 -1.106433697881073 1110 62-63 3.2108647565884354 1110 64-65 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9.167054947976002 2117 9 12.209736462368038 2117 10-11 6.253694843168528 2117 12-13 0.7334709880762524 2117 14-15 0.5562224690514164 2117 16-17 -0.18513281309334317 2117 18-19 -9.84732285258601 2117 20-21 0.8482375446191242 2117 22-23 -1.4276543631806788 2117 24-25 1.6950648401306303 2117 26-27 -6.49058156755525 2117 28-29 5.288642534366222 2117 30-31 2.2828572377914504 2117 32-33 -3.2156119465329986 2117 34-35 9.69373291182502 2117 36-37 2.843339409128884 2117 38-39 1.2464242993848238 2117 40-41 -0.05398629148628942 2117 42-43 5.459990411635154 2117 44-45 -2.076203766993242 2117 46-47 -2.9818860598465875 2117 48-49 -7.9061621857674425 2117 50-51 1.7224866142629338 2117 52-53 -1.085753208779522 2117 54-55 -6.240957982076402 2117 56-57 6.488493487506645 2117 58-59 -2.7538225298093693 2117 60-61 3.060232968785595 2117 62-63 -1.8724685767448968 2117 64-65 5.5623272195640645 2117 66-67 0.90772575377839 2117 68-69 8.64949969621022 2117 70-71 12.593008563074356 2117 72-73 14.855219013442696 2117 74-75 -3.0583347573479145 2117 76 15.889246791220472 2118 1 4.581035923141187 2118 2 -9.42641547049443 2118 3 -5.13555764411028 2118 4 -0.8916258449153212 2118 5 7.616772043745723 2118 6 -2.9438036378825885 2118 7 -2.3562590187590224 2118 8 -3.999611718690666 2118 9 5.543069795701371 2118 10-11 -6.246305156831472 2118 12-13 -7.433195678590415 2118 14-15 -0.44377753094858363 2118 16-17 -6.101799479760011 2118 18-19 -7.847322852586011 2118 20-21 -5.318429122047544 2118 22-23 -3.927654363180679 2118 24-25 -1.8049351598693697 2118 26-27 2.0927517657780825 2118 28-29 -3.211357465633778 2118 30-31 -3.8838094288752156 2118 32-33 -6.715611946532999 2118 34-35 -9.30626708817498 2118 36-37 -5.906660590871116 2118 38-39 -4.003575700615176 2118 40-41 -6.637319624819622 2118 42-43 -1.1233429216981818 2118 44-45 -8.326203766993242 2118 46-47 -7.06521939317992 2118 48-49 -5.4061621857674425 2118 50-51 -7.860846719070398 2118 52-53 -6.669086542112854 2118 54-55 -2.7409579820764023 2118 56-57 -7.928173179160023 2118 58-59 -6.837155863142701 2118 60-61 -8.439767031214405 2118 62-63 -6.122468576744897 2118 64-65 -0.4376727804359355 2118 66-67 -1.425607579554942 2118 68-69 -3.1005003037897794 2118 70-71 1.759675229741024 2118 72-73 -4.061447653223972 2118 74-75 -4.391668090681248 2118 76 -2.44408654211286 2119 1 1.4143692564745187 2119 2 2.5735845295055704 2119 3 3.031109022556386 2119 4 3.2750408217513467 2119 5 7.7834387104123905 2119 6 2.8895296954507437 2119 7 8.143740981240978 2119 8 1.167054947976002 2119 9 6.7097364623680384 2119 10-11 14.003694843168528 2119 12-13 6.983470988076252 2119 14-15 8.556222469051416 2119 16-17 -2.685132813093343 2119 18-19 6.152677147413989 2119 20-21 3.348237544619124 2119 22-23 13.572345636819321 2119 24-25 8.69506484013063 2119 26-27 9.25941843244475 2119 28-29 2.538642534366222 2119 30-31 2.5328572377914504 2119 32-33 4.284388053467001 2119 34-35 3.6937329118250197 2119 36-37 2.093339409128884 2119 38-39 -0.2535757006151762 2119 40-41 4.696013708513711 2119 42-43 -1.7900095883648461 2119 44-45 7.423796233006758 2119 46-47 -2.4818860598465875 2119 48-49 -1.4061621857674425 2119 50-51 1.9724866142629338 2119 52-53 -0.5857532087795221 2119 54-55 -8.990957982076402 2119 56-57 -2.511506512493355 2119 58-59 2.7461774701906307 2119 60-61 -1.689767031214405 2119 62-63 -5.122468576744897 2119 64-65 -2.6876727804359355 2119 66-67 -3.59227424622161 2119 68-69 3.1494996962102206 2119 70-71 -0.9069914369256438 2119 72-73 -2.644780986557304 2119 74-75 -0.8083347573479145 2119 76 -2.1107532087795278 >>END_MODULE >>Per sequence quality scores fail #Quality Count 12 7.0 13 48.0 14 143.0 15 216.0 16 265.0 17 327.0 18 378.0 19 351.0 20 279.0 21 216.0 22 132.0 23 102.0 24 72.0 25 41.0 26 36.0 27 22.0 28 21.0 29 22.0 30 9.0 31 11.0 32 14.0 33 16.0 34 18.0 35 32.0 36 39.0 37 43.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 7.942110836569008 28.66219555241793 6.918460995411224 56.477232615601835 2 3.6363636363636362 23.846153846153847 43.63636363636363 28.881118881118883 3 19.02097902097902 26.18881118881119 26.153846153846157 28.636363636363637 4 5.20979020979021 25.27972027972028 36.81818181818181 32.69230769230769 5 4.580419580419581 28.951048951048953 40.87412587412587 25.594405594405593 6 5.419580419580419 47.51748251748252 13.566433566433567 33.4965034965035 7 4.755244755244755 53.671328671328666 11.083916083916085 30.48951048951049 8 4.335664335664335 37.0979020979021 14.125874125874127 44.44055944055944 9 10.20979020979021 30.76923076923077 12.237762237762238 46.78321678321679 10-11 7.674825174825174 32.15034965034965 24.17832167832168 35.99650349650349 12-13 6.276223776223776 28.321678321678323 24.108391608391607 41.29370629370629 14-15 12.115384615384615 32.552447552447546 18.181818181818183 37.15034965034965 16-17 16.32867132867133 31.538461538461537 17.22027972027972 34.91258741258741 18-19 11.818181818181818 27.692307692307693 16.94055944055944 43.548951048951054 20-21 10.926573426573427 30.174825174825177 17.98951048951049 40.909090909090914 22-23 18.53146853146853 28.54895104895105 15.402097902097903 37.51748251748252 24-25 13.479020979020978 35.90909090909091 14.318181818181818 36.29370629370629 26-27 11.678321678321678 36.24125874125874 19.632867132867133 32.44755244755245 28-29 15.192307692307692 31.083916083916087 23.583916083916083 30.13986013986014 30-31 14.073426573426573 29.475524475524477 24.353146853146853 32.0979020979021 32-33 10.472027972027972 36.1013986013986 21.835664335664333 31.590909090909093 34-35 9.965034965034965 35.05244755244755 21.083916083916083 33.8986013986014 36-37 11.328671328671328 29.65034965034965 23.776223776223777 35.24475524475525 38-39 12.36013986013986 24.93006993006993 27.93706293706294 34.77272727272727 40-41 8.531468531468532 27.132867132867133 25.734265734265733 38.6013986013986 42-43 6.136363636363637 26.11888111888112 28.671328671328673 39.07342657342657 44-45 7.115384615384615 25.82167832167832 31.625874125874127 35.43706293706293 46-47 7.1328671328671325 31.066433566433567 26.64335664335664 35.15734265734265 48-49 7.8671328671328675 34.7027972027972 19.72027972027972 37.709790209790214 50-51 7.4125874125874125 32.290209790209786 20.454545454545457 39.84265734265735 52-53 7.167832167832168 31.835664335664337 21.88811188811189 39.10839160839161 54-55 7.255244755244755 31.45104895104895 19.213286713286713 42.08041958041958 56-57 11.555944055944057 32.34265734265735 18.234265734265733 37.86713286713287 58-59 9.825174825174825 29.94755244755245 19.178321678321677 41.04895104895105 60-61 7.307692307692308 27.150349650349646 20.472027972027973 45.06993006993007 62-63 9.739464941423325 30.494841755551672 17.712886868333623 42.052806434691384 64-65 9.597902097902097 30.48951048951049 15.034965034965033 44.87762237762238 66-67 8.181818181818182 38.07692307692307 11.993006993006992 41.74825174825175 68-69 10.821678321678322 37.04545454545455 12.132867132867133 40.0 70-71 11.52097902097902 39.54545454545455 11.765734265734265 37.16783216783217 72-73 9.073426573426573 36.99300699300699 10.62937062937063 43.30419580419581 74-75 9.335664335664335 37.16783216783217 12.692307692307692 40.8041958041958 76 6.7482517482517475 30.629370629370626 13.916083916083915 48.70629370629371 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 0.5 16 1.0 17 2.0 18 1.5 19 1.5 20 3.0 21 4.0 22 5.0 23 6.0 24 5.0 25 4.0 26 8.5 27 12.0 28 16.5 29 22.0 30 21.0 31 23.5 32 34.0 33 41.0 34 42.0 35 48.5 36 58.0 37 62.0 38 74.5 39 96.5 40 118.0 41 129.0 42 128.0 43 137.5 44 159.5 45 183.5 46 195.0 47 183.0 48 178.5 49 190.0 50 194.0 51 173.5 52 138.5 53 129.0 54 134.0 55 128.5 56 111.0 57 91.5 58 84.0 59 71.0 60 62.0 61 54.0 62 42.0 63 35.5 64 29.0 65 22.5 66 16.0 67 16.0 68 15.5 69 13.0 70 7.0 71 3.0 72 4.5 73 4.0 74 1.5 75 1.0 76 1.5 77 1.5 78 1.5 79 2.0 80 2.0 81 1.5 82 0.5 83 0.0 84 0.5 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 1.0 92 2.0 93 1.0 94 0.5 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.9440559440559441 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.017482517482517484 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 2860.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.0909090909091 #Duplication Level Percentage of deduplicated Percentage of total 1 99.40014114326041 98.4965034965035 2 0.3881439661256175 0.7692307692307693 3 0.14114326040931546 0.4195804195804196 4 0.035285815102328866 0.13986013986013987 5 0.035285815102328866 0.17482517482517482 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTG 5 0.17482517482517482 No Hit CAAAAGGAAACATATGTTCACAGTGGGAAGGATTATTACTCTGTCTCTTA 4 0.13986013986013987 No Hit CTGTAATGCCAGCACTTTGGAAAGACGAGGAGGGCAGATCACTTCTGTCT 3 0.1048951048951049 No Hit CTGTCTCTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATC 3 0.1048951048951049 No Hit CCATGAATCCAACAGCCCAGTGGATGAAATTTTGCTTATGTGTATTTCAC 3 0.1048951048951049 No Hit GGTTCTTACCTCCTGGTATTAATGCCGTTGTATAATCTCCCCATCTTGAG 3 0.1048951048951049 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.13986013986013987 0.0 2 0.0 0.0 0.0 0.13986013986013987 0.0 3 0.0 0.0 0.0 0.13986013986013987 0.0 4 0.0 0.0 0.0 0.13986013986013987 0.0 5 0.0 0.0 0.0 0.13986013986013987 0.0 6 0.0 0.0 0.0 0.13986013986013987 0.0 7 0.0 0.0 0.0 0.13986013986013987 0.0 8 0.0 0.0 0.0 0.13986013986013987 0.0 9 0.0 0.0 0.0 0.13986013986013987 0.0 10 0.0 0.0 0.0 0.13986013986013987 0.0 11 0.0 0.0 0.0 0.13986013986013987 0.0 12 0.0 0.0 0.0 0.13986013986013987 0.0 13 0.0 0.0 0.0 0.13986013986013987 0.0 14 0.0 0.0 0.0 0.13986013986013987 0.0 15 0.0 0.0 0.0 0.13986013986013987 0.0 16 0.0 0.0 0.0 0.13986013986013987 0.0 17 0.0 0.0 0.0 0.2097902097902098 0.0 18 0.0 0.0 0.0 0.27972027972027974 0.0 19 0.0 0.0 0.0 0.48951048951048953 0.0 20 0.0 0.0 0.0 0.5594405594405595 0.0 21 0.0 0.0 0.0 0.5594405594405595 0.0 22 0.0 0.0 0.0 0.5594405594405595 0.0 23 0.0 0.0 0.0 0.5944055944055944 0.0 24 0.0 0.0 0.0 0.5944055944055944 0.0 25 0.0 0.0 0.0 0.5944055944055944 0.0 26 0.0 0.0 0.0 0.5944055944055944 0.0 27 0.0 0.0 0.0 0.5944055944055944 0.0 28 0.0 0.0 0.0 0.6293706293706294 0.0 29 0.0 0.0 0.0 0.6643356643356644 0.0 30 0.0 0.0 0.0 0.6643356643356644 0.0 31 0.0 0.0 0.0 0.6993006993006993 0.0 32 0.0 0.0 0.0 0.7342657342657343 0.0 33 0.0 0.0 0.0 0.8041958041958042 0.0 34 0.0 0.0 0.0 0.8391608391608392 0.0 35 0.0 0.0 0.0 0.9440559440559441 0.0 36 0.0 0.0 0.0 1.048951048951049 0.0 37 0.0 0.0 0.0 1.048951048951049 0.0 38 0.0 0.0 0.0 1.1538461538461537 0.0 39 0.0 0.0 0.0 1.2237762237762237 0.0 40 0.0 0.0 0.0 1.3286713286713288 0.0 41 0.0 0.0 0.0 1.7132867132867133 0.0 42 0.0 0.0 0.0 1.8531468531468531 0.0 43 0.0 0.0 0.0 2.097902097902098 0.0 44 0.0 0.0 0.0 2.3426573426573425 0.0 45 0.0 0.0 0.0 2.6223776223776225 0.0 46 0.0 0.0 0.0 2.762237762237762 0.0 47 0.0 0.0 0.0 2.867132867132867 0.0 48 0.0 0.0 0.0 2.902097902097902 0.0 49 0.0 0.0 0.0 2.902097902097902 0.0 50 0.0 0.0 0.0 2.937062937062937 0.0 51 0.0 0.0 0.0 3.041958041958042 0.0 52 0.0 0.0 0.0 3.041958041958042 0.0 53 0.0 0.0 0.0 3.3216783216783217 0.0 54 0.0 0.0 0.0 3.3566433566433567 0.0 55 0.0 0.0 0.0 3.4965034965034967 0.0 56 0.0 0.0 0.0 3.6013986013986012 0.0 57 0.0 0.0 0.0 3.6013986013986012 0.0 58 0.0 0.0 0.0 3.7062937062937062 0.0 59 0.0 0.0 0.0 3.7762237762237763 0.0 60 0.0 0.0 0.0 3.7762237762237763 0.0 61 0.0 0.0 0.0 3.8111888111888113 0.0 62 0.0 0.0 0.0 3.8461538461538463 0.0 63 0.0 0.0 0.0 3.9510489510489513 0.0 64 0.0 0.0 0.0 3.9510489510489513 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE