FastQCFastQC Report
Wed 25 May 2016
SRR1780588_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780588_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2860
Sequences flagged as poor quality0
Sequence length76
%GC42

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT69724.37062937062937No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT48216.853146853146853Illumina PCR Primer Index 9 (95% over 22bp)
CTTTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC842.937062937062937No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG702.4475524475524475No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC602.097902097902098No Hit
TATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCT521.8181818181818181Illumina PCR Primer Index 9 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGT401.3986013986013985No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGT391.3636363636363635No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCGT391.3636363636363635No Hit
CTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC341.1888111888111887No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGTCTT230.8041958041958042No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCG190.6643356643356644No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC180.6293706293706294No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCGTCTT170.5944055944055944No Hit
CTTATACACATCTCCGAGCCCACGCGACGTTAACAGATCTCGTATGCCGT170.5944055944055944No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGTCTT170.5944055944055944No Hit
ACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTTCT150.5244755244755245Illumina PCR Primer Index 9 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC140.48951048951048953No Hit
ATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTTCTGCTT120.4195804195804196Illumina PCR Primer Index 9 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCAGT120.4195804195804196No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCC120.4195804195804196No Hit
CTTATACACATCTCCGAGCCCAAGAGACGTTAACAGATCTCGTATGCCGT110.38461538461538464No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTAGTATGCCGT110.38461538461538464No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGCTCTCGTATGCCGT100.34965034965034963No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCG90.3146853146853147No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG80.27972027972027974No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAAAAGATCTCGTATGCCGT70.24475524475524477No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC70.24475524475524477No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTCTGCCGTCTT70.24475524475524477No Hit
CTTATACACATATCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT70.24475524475524477No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCCCGTATGCCGT70.24475524475524477No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGC70.24475524475524477No Hit
CTTATAACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG70.24475524475524477No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGACATCGTATGCCGT60.2097902097902098No Hit
ATACACATCTCCGAGCCCACGAGACGTTCACAGATCTCGTATGCCGTCTT60.2097902097902098TruSeq Adapter, Index 9 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCACGTATGCCGT60.2097902097902098No Hit
CTTATTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG60.2097902097902098No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCC60.2097902097902098No Hit
CTTATACACATCTCCGAGCCCACGAGCCGTTAACAGATCTCGTATGCCGT50.17482517482517482No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGAGCTCGTATGCCGTCTT50.17482517482517482No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAAATCGTATGCCGT50.17482517482517482No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAGCTCGTATGCCGT50.17482517482517482No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGCTCTCGTATGCCGTCTT50.17482517482517482No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGACGT50.17482517482517482No Hit
CTTATCACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC50.17482517482517482No Hit
CTTATACACATCTCCGAGCCAACGAGACGTTAACAGATCTCGTATGCCGT50.17482517482517482No Hit
ATTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT40.13986013986013987No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTACCAGATCTCGTATGCCGT40.13986013986013987No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCACAGATCTCGTATGCCGT40.13986013986013987No Hit
TATCACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT40.13986013986013987Illumina PCR Primer Index 9 (95% over 22bp)
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTA40.13986013986013987No Hit
ATACACATCTCCGAGCCCACGAGACGTTACCAGATCTCGTATGCCGTCTT40.13986013986013987Illumina PCR Primer Index 9 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGTGACGTTAACAGATCTCGTATGCCGT40.13986013986013987No Hit
CTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTTCTGCTTGA40.13986013986013987Illumina PCR Primer Index 9 (96% over 29bp)
CTTATACACATCTACGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT40.13986013986013987No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTAACAGATCTCGTATGCCGT40.13986013986013987No Hit
ATACACATCTACGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT40.13986013986013987Illumina PCR Primer Index 9 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGA40.13986013986013987No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCCT40.13986013986013987No Hit
ATACACATCTCCGAGCACACGAGACGTTAACAGATCTCGTATGCCGTCTT40.13986013986013987TruSeq Adapter, Index 9 (95% over 22bp)
CTTATACACATCTGACGCCTGTCTCTTATACACATCTCCGAGCCCACGAG30.1048951048951049No Hit
CTTATACACATCTCCGAGACCACGAGACGTTAACAGATATCGTATGCCGT30.1048951048951049No Hit
AGTAATAATCCTTCCCACTGTGAACATATGTTTCCTTTTGCTGTCTCTTA30.1048951048951049No Hit
CTTATACACATCTCCGAGCACACGAGACGTTAACAGATCTCGTATGCCGT30.1048951048951049No Hit
TACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTTC30.1048951048951049Illumina PCR Primer Index 9 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACATTAACAGATCTCGTATGCCGT30.1048951048951049No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTCTGCCGT30.1048951048951049No Hit
TATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCGTCT30.1048951048951049No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG30.1048951048951049No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCC30.1048951048951049No Hit
CTTAACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC30.1048951048951049No Hit
TATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGTCT30.1048951048951049No Hit
ATACACATCTCCGAGCCCACGAGCCGTTAACAGATCTCGTATGCCGTCTT30.1048951048951049Illumina PCR Primer Index 9 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCG30.1048951048951049No Hit
CTTTACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGTC30.1048951048951049No Hit
CTTATACACATCTCCGAGCCCACGAGAAGTTAACAGATCTCGTATGCCGT30.1048951048951049No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGACATCGTATGCC30.1048951048951049No Hit
TGTAAAGAATTTGGGTGTGAAATACACATAAGCAAAATTTCATCCACTGG30.1048951048951049No Hit
ATACACCTCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT30.1048951048951049Illumina PCR Primer Index 9 (95% over 22bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTACA150.002003462370.01
ACAGACC150.002003462370.033
CCTCGTA150.002003462370.038
TTACACA150.002003462370.03
ACCTCGT150.002003462370.037
CAGACCT150.002003462370.034
AGACCTC150.002003462370.035
AACAGAC150.002003462370.032
GACCTCG150.002003462370.036
TTTACAC150.002003462370.02
CTTATAC1350.044.0740741
TTATACA1500.042.02
TCTTATA502.4111068E-642.01
TATACAC1650.040.3030323
ATACACA2250.029.5555554
AGACGTT2550.028.82352825
CGTATGC2550.028.82352841
TCGTATG2550.028.82352840
ATCTCCG2700.028.51851810
CATCTCC2700.028.5185189