##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780588_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2860 Sequences flagged as poor quality 0 Sequence length 76 %GC 42 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.2479020979021 34.0 34.0 34.0 27.0 34.0 2 32.78986013986014 34.0 34.0 34.0 31.0 34.0 3 33.08181818181818 34.0 34.0 34.0 32.0 34.0 4 33.41748251748252 34.0 34.0 34.0 33.0 34.0 5 33.44755244755245 34.0 34.0 34.0 34.0 34.0 6 37.17412587412588 38.0 37.0 38.0 36.0 38.0 7 36.58846153846154 38.0 37.0 38.0 34.0 38.0 8 36.79755244755245 38.0 37.0 38.0 34.0 38.0 9 36.41783216783217 38.0 37.0 38.0 34.0 38.0 10-11 36.06800699300699 38.0 36.5 38.0 32.5 38.0 12-13 35.78618881118881 38.0 36.0 38.0 31.0 38.0 14-15 35.70192307692308 37.5 36.0 38.0 31.0 38.0 16-17 35.67832167832168 38.0 36.0 38.0 31.0 38.0 18-19 35.65472027972028 38.0 35.5 38.0 31.0 38.0 20-21 35.36783216783216 38.0 36.0 38.0 31.0 38.0 22-23 35.49527972027972 37.5 35.0 38.0 31.0 38.0 24-25 33.94353146853147 37.0 32.5 38.0 23.5 38.0 26-27 34.07517482517483 37.0 33.5 38.0 25.0 38.0 28-29 34.46223776223776 37.0 34.5 38.0 28.0 38.0 30-31 34.18496503496503 37.0 34.0 38.0 25.5 38.0 32-33 34.78234265734265 37.0 34.5 38.0 27.0 38.0 34-35 33.49947552447553 37.0 33.0 38.0 19.0 38.0 36-37 33.10541958041958 37.0 34.0 38.0 21.5 38.0 38-39 29.481293706293705 34.0 25.0 37.0 11.0 38.0 40-41 31.993006993006993 35.0 30.5 37.0 16.5 38.0 42-43 34.26346153846154 37.0 34.0 38.0 26.5 38.0 44-45 34.84562937062937 37.0 34.0 38.0 29.0 38.0 46-47 34.64335664335664 37.0 34.0 38.0 27.0 38.0 48-49 34.08496503496504 37.0 34.0 38.0 25.0 38.0 50-51 34.02972027972028 37.0 33.5 38.0 24.5 38.0 52-53 33.60576923076923 37.0 34.0 38.0 23.0 38.0 54-55 34.1743006993007 37.0 34.0 38.0 25.5 38.0 56-57 34.195279720279714 37.0 34.0 38.0 25.0 38.0 58-59 33.854195804195804 37.0 34.0 38.0 24.0 38.0 60-61 33.35804195804196 37.0 34.0 38.0 22.5 38.0 62-63 34.367482517482514 37.0 34.0 38.0 26.0 38.0 64-65 34.58706293706294 37.0 34.0 38.0 26.0 38.0 66-67 32.290209790209786 36.5 31.0 38.0 21.0 38.0 68-69 28.38339160839161 32.5 21.5 37.0 11.0 38.0 70-71 24.809265734265736 27.0 16.0 35.0 10.5 37.5 72-73 20.61958041958042 21.5 11.0 26.5 11.0 35.0 74-75 20.228496503496505 21.0 11.0 26.0 11.0 34.0 76 20.713636363636365 21.0 11.0 27.0 11.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.6548815219867805 1101 2 0.26011999696211063 1101 3 1.041435976304399 1101 4 0.48473551302498663 1101 5 0.5988826232247249 1101 6 -1.0021550087339648 1101 7 0.5650404420141228 1101 8 0.7615449608870648 1101 9 1.1838307890939461 1101 10-11 1.351679387863598 1101 12-13 1.4794495708969393 1101 14-15 1.6347919989367412 1101 16-17 0.6182639363560369 1101 18-19 1.5248314916078058 1101 20-21 1.7359587415508457 1101 22-23 0.6030402711323717 1101 24-25 0.7226641414141426 1101 26-27 1.4230134996582393 1101 28-29 1.9478592314118686 1101 30-31 1.7859734563681826 1101 32-33 1.4329141983747178 1101 34-35 3.946861946533005 1101 36-37 2.1539274322168964 1101 38-39 -3.273800505050506 1101 40-41 1.6978492633097986 1101 42-43 0.10987221842484729 1101 44-45 0.781055384673806 1101 46-47 2.026351389838233 1101 48-49 -0.34471120984279224 1101 50-51 0.8974832915622386 1101 52-53 -3.5791913495860825 1101 54-55 1.0047870623528539 1101 56-57 -4.776593946988683 1101 58-59 -1.666597839295214 1101 60-61 -2.570855168223588 1101 62-63 -0.09032856763120378 1101 64-65 1.1853867623604515 1101 66-67 0.10815105946684156 1101 68-69 2.3495400432900375 1101 70-71 -5.419923862687021 1101 72-73 -4.077936792739425 1101 74-75 0.9885091516670457 1101 76 -0.3442384370015965 1102 1 -1.6104370775423362 1102 2 0.5267866636287764 1102 3 1.041435976304399 1102 4 0.48473551302498663 1102 5 0.5988826232247249 1102 6 -0.024377230956183382 1102 7 -1.3571817802081014 1102 8 -0.6940105946684909 1102 9 1.006053011316169 1102 10-11 0.9350127211969266 1102 12-13 1.8127829042302679 1102 14-15 0.46256977671451693 1102 16-17 0.46826393635603836 1102 18-19 0.3637203804966944 1102 20-21 2.3081809637730686 1102 22-23 1.5363736044657017 1102 24-25 -6.766224747474745 1102 26-27 -2.865875389230645 1102 28-29 0.41452589807853 1102 30-31 1.7581956785904111 1102 32-33 -0.6226413571808393 1102 34-35 2.9801952798663365 1102 36-37 -2.140517012227548 1102 38-39 -0.2404671717171709 1102 40-41 3.1422937077542414 1102 42-43 2.254316662869293 1102 44-45 2.208833162451583 1102 46-47 1.25968472317156 1102 48-49 -2.483600098731678 1102 50-51 -0.29696115288220426 1102 52-53 -0.4569691273638554 1102 54-55 2.188120395686184 1102 56-57 1.5789616085668712 1102 58-59 1.7111799384825588 1102 60-61 -6.076410723779144 1102 62-63 -1.573661900964531 1102 64-65 1.64649787347156 1102 66-67 2.4137066150223987 1102 68-69 -3.139348845598853 1102 70-71 -4.992146084909244 1102 72-73 -0.13349234829498258 1102 74-75 0.8940647072226042 1102 76 -2.4775717703349294 1103 1 0.516835649730389 1103 2 -1.073213336371225 1103 3 -1.958564023695601 1103 4 0.12109914938861976 1103 5 0.235246259588358 1103 6 0.5028954963165404 1103 7 -0.20263632566264533 1103 8 0.5696257689678745 1103 9 0.6787802840434409 1103 10-11 0.9122854484696532 1103 12-13 -0.6872170957697321 1103 14-15 -0.7692484051036601 1103 16-17 0.4819002999923967 1103 18-19 0.686447653223965 1103 20-21 -2.178182672590573 1103 22-23 -0.06362639553429261 1103 24-25 -2.7571338383838366 1103 26-27 -0.5113299346851932 1103 28-29 -2.8400195564669204 1103 30-31 -0.2645315941368622 1103 32-33 -0.03173226627174586 1103 34-35 -1.4925319928609397 1103 36-37 -0.7905170122275464 1103 38-39 -4.94046717171717 1103 40-41 -2.812251746791212 1103 42-43 -0.35477424622161635 1103 44-45 1.1951967988152248 1103 46-47 1.9051392686261082 1103 48-49 -4.960872826004405 1103 50-51 -1.910597516518564 1103 52-53 -2.4478782182729546 1103 54-55 -4.843697786131994 1103 56-57 -0.7664929368876727 1103 58-59 -2.7524564251538024 1103 60-61 -10.171865269233688 1103 62-63 -0.8327528100554389 1103 64-65 0.432861509835206 1103 66-67 -2.154475203159418 1103 68-69 2.9151966089466015 1103 70-71 4.130581187818031 1103 72-73 -2.588037802840436 1103 74-75 -2.592298929141034 1103 76 -3.2230263157894754 1104 1 -0.2937704108756698 1104 2 0.5934533302954392 1104 3 0.4581026429710633 1104 4 -2.181931153641681 1104 5 -1.0677840434419394 1104 6 0.19228943571047807 1104 7 1.0928182197919014 1104 8 1.2059894053315077 1104 9 1.4060530113161676 1104 10-11 0.5183460545302552 1104 12-13 1.0211162375636036 1104 14-15 1.5792364433811912 1104 16-17 0.11826393635603694 1104 18-19 0.7887203804966916 1104 20-21 0.12484763043973857 1104 22-23 0.7697069377990431 1104 24-25 0.12544191919192116 1104 26-27 -0.21587538923064642 1104 28-29 2.114525898078533 1104 30-31 1.8831956785904111 1104 32-33 2.169025309485832 1104 34-35 2.1135286131996622 1104 36-37 4.0844829877724536 1104 38-39 1.476199494949494 1104 40-41 2.7672937077542414 1104 42-43 1.9709833295359545 1104 44-45 1.9338331624515845 1104 46-47 3.276351389838233 1104 48-49 -0.9419334320650066 1104 50-51 0.41137218045113144 1104 52-53 2.184697539302803 1104 54-55 2.379787062352854 1104 56-57 1.6122949419002026 1104 58-59 -0.8888200615174355 1104 60-61 -2.209744057112477 1104 62-63 0.03467143236880332 1104 64-65 1.6714978734715658 1104 66-67 2.330373281689063 1104 68-69 4.543984487734477 1104 70-71 3.8995205817574217 1104 72-73 1.3665076517050174 1104 74-75 4.335731373889267 1104 76 -0.3442384370015965 1105 1 1.2895629224576624 1105 2 0.9267866636287749 1105 3 1.041435976304399 1105 4 -1.4319311536416777 1105 5 0.5988826232247249 1105 6 -0.057710564289521926 1105 7 -0.1571817802080986 1105 8 -1.4606772613351566 1105 9 -0.010613655350496742 1105 10-11 1.1016793878635909 1105 12-13 0.8961162375636036 1105 14-15 1.0375697767145198 1105 16-17 0.9932639363560369 1105 18-19 1.8720537138300202 1105 20-21 2.083180963773067 1105 22-23 0.3530402711323788 1105 24-25 2.5421085858585926 1105 26-27 2.075791277436018 1105 28-29 0.7395258980785329 1105 30-31 2.008195678590411 1105 32-33 2.669025309485825 1105 34-35 1.821861946532998 1105 36-37 3.751149654439118 1105 38-39 -0.5654671717171702 1105 40-41 1.7672937077542414 1105 42-43 1.3043166628692902 1105 44-45 2.5588331624515845 1105 46-47 3.1513513898382257 1105 48-49 2.8080665679349934 1105 50-51 3.494705513784467 1105 52-53 2.9763642059694675 1105 54-55 3.379787062352861 1105 56-57 2.278961608566874 1105 58-59 2.6111799384825645 1105 60-61 2.123589276220855 1105 62-63 -0.2986619009645324 1105 64-65 2.213164540138237 1105 66-67 4.038706615022399 1105 68-69 6.835651154401148 1105 70-71 3.3995205817574217 1105 72-73 1.9915076517050174 1105 74-75 -0.7059352927773972 1105 76 -1.9275717703349287 1106 1 -1.483164350269611 1106 2 -0.34594060909849844 1106 3 -0.0494731146046945 1106 4 -0.6061735778841069 1106 5 -2.492026467684365 1106 6 -0.3152863218652797 1106 7 -3.4753635983899187 1106 8 -0.7940105946684923 1106 9 -2.9575833523201993 1106 10-11 0.13955817574237983 1106 12-13 2.085510176957534 1106 14-15 -0.08743022328548022 1106 16-17 -1.6999178818257832 1106 18-19 0.2319021986785117 1106 20-21 1.3672718728639737 1106 22-23 -0.5636263955342926 1106 24-25 -1.7571338383838366 1106 26-27 -0.3295117528670062 1106 28-29 -0.7036559201032873 1106 30-31 -1.309986139591409 1106 32-33 0.6955404610009737 1106 34-35 4.9620134616845135 1106 36-37 4.345846624136094 1106 38-39 -4.75864898989899 1106 40-41 -1.0395244740639384 1106 42-43 2.05431666286929 1106 44-45 1.6042877079061313 1106 46-47 3.0415029049897484 1106 48-49 2.8573089921774155 1106 50-51 -0.45605206197311077 1106 52-53 -0.2660600364547676 1106 54-55 0.7017567593225493 1106 56-57 1.5516888812941474 1106 58-59 0.8384526657552911 1106 60-61 2.5099529125844953 1106 62-63 2.030883553580921 1106 64-65 -1.2035021265284342 1106 66-67 -0.2453842940685078 1106 68-69 6.142469336219328 1106 70-71 0.7669448241816674 1106 72-73 -0.7244014392040761 1106 74-75 -0.41048074732285045 1106 76 4.413337320574161 1107 1 1.7062295891243267 1107 2 -0.9898800030378894 1107 3 0.1247693096377347 1107 4 -0.18193115364167767 1107 5 0.5988826232247249 1107 6 0.19228943571047807 1107 7 1.0094848864585657 1107 8 -0.12734392800182803 1107 9 -0.26061365535049674 1107 10-11 0.14334605453025517 1107 12-13 -0.10388376243640352 1107 14-15 -0.045763556618815926 1107 16-17 1.118263936356037 1107 18-19 0.9137203804966916 1107 20-21 1.0831809637730672 1107 22-23 0.7280402711323717 1107 24-25 1.8754419191919283 1107 26-27 1.4924579441026893 1107 28-29 2.3228592314118615 1107 30-31 1.9665290119237397 1107 32-33 2.419025309485832 1107 34-35 2.9051952798663336 1107 36-37 4.2094829877724536 1107 38-39 -0.2738005050505059 1107 40-41 -0.35770629224575856 1107 42-43 0.8876499962026259 1107 44-45 0.47549982911824884 1107 46-47 2.3596847231715614 1107 48-49 -0.025266765398349378 1107 50-51 1.4947055137844671 1107 52-53 0.8513642059694746 1107 54-55 -0.03687960431381043 1107 56-57 0.4456282752335383 1107 58-59 -1.0554867281840998 1107 60-61 2.2902559428875193 1107 62-63 2.868004765702132 1107 64-65 2.963164540138237 1107 66-67 2.1637066150223987 1107 68-69 6.543984487734484 1107 70-71 1.8578539150907574 1107 72-73 1.0748409850383531 1107 74-75 -0.1642686261107329 1107 76 -0.5942384370015965 1108 1 -1.5104370775423384 1108 2 0.5267866636287764 1108 3 1.041435976304399 1108 4 0.48473551302498663 1108 5 0.5988826232247249 1108 6 -0.1243772309561848 1108 7 0.5428182197919043 1108 8 0.9059894053315105 1108 9 -2.0939469886838324 1108 10-11 1.1350127211969294 1108 12-13 2.312782904230268 1108 14-15 1.212569776714517 1108 16-17 -0.18173606364396022 1108 18-19 0.8637203804966944 1108 20-21 2.40818096377307 1108 22-23 0.8863736044657102 1108 24-25 1.1837752525252512 1108 26-27 1.884124610769355 1108 28-29 -0.13547410192146714 1108 30-31 2.708195678590414 1108 32-33 2.3273586428191635 1108 34-35 3.180195279866332 1108 36-37 4.009482987772451 1108 38-39 1.0095328282828326 1108 40-41 1.0422937077542471 1108 42-43 0.054316662869290155 1108 44-45 2.0088331624515874 1108 46-47 2.8096847231715643 1108 48-49 3.1163999012683234 1108 50-51 3.55303884711779 1108 52-53 0.943030872636136 1108 54-55 1.688120395686191 1108 56-57 1.778961608566874 1108 58-59 2.26117993848257 1108 60-61 2.7235892762208564 1108 62-63 3.3263380990354676 1108 64-65 2.396497873471567 1108 66-67 3.7637066150224 1108 68-69 3.860651154401147 1108 70-71 4.257853915090756 1108 72-73 2.1665076517050146 1108 74-75 0.7940647072226028 1108 76 -2.9775717703349294 1109 1 -2.7558916229968844 1109 2 0.017695754537868424 1109 3 -0.958564023695601 1109 4 -0.24253721424774 1109 5 0.3261553504974515 1109 6 0.4119864054074469 1109 7 -0.3844545074808252 1109 8 0.9332621326042343 1109 9 1.2242348294979877 1109 10-11 -0.042260006075807155 1109 12-13 -1.2781261866788256 1109 14-15 0.867115231259973 1109 16-17 0.6637184818105837 1109 18-19 0.9137203804966916 1109 20-21 2.2309082365003334 1109 22-23 0.2545554226475275 1109 24-25 -3.075315656565653 1109 26-27 -0.9204208437760997 1109 28-29 -1.1582013746487405 1109 30-31 -0.08271341231868234 1109 32-33 -0.4862777208171991 1109 34-35 0.8711043707754271 1109 36-37 3.5731193514088133 1109 38-39 0.33226010101010317 1109 40-41 -4.630433564973032 1109 42-43 -0.26386515531252996 1109 44-45 0.28610588972431117 1109 46-47 0.7687756322624679 1109 48-49 0.22094535581377528 1109 50-51 1.1803115743905224 1109 52-53 2.3703035999088655 1109 54-55 -1.252788695222904 1109 56-57 1.2335070631123273 1109 58-59 -0.4342746069719823 1109 60-61 -0.944592541960958 1109 62-63 -1.3782073555099856 1109 64-65 2.1146796916533788 1109 66-67 0.2546157059314922 1109 68-69 -2.6302579365079417 1109 70-71 2.0396720969089372 1109 72-73 2.7755985607959275 1109 74-75 2.498610161768056 1109 76 0.958791866028708 1110 1 -8.321548188653448 1110 2 -2.9621022252601144 1110 3 -1.6252306903622689 1110 4 0.2625132908027652 1110 5 -0.845561821219718 1110 6 -0.7799327865117434 1110 7 0.7872626642363443 1110 8 0.872656071998172 1110 9 0.9616085668717247 1110 10-11 -0.7038761676919592 1110 12-13 1.6461162375635965 1110 14-15 1.468125332270077 1110 16-17 1.173819491911594 1110 18-19 0.6914981582744701 1110 20-21 1.847069852661953 1110 22-23 0.9363736044657074 1110 24-25 -0.4440025252525146 1110 26-27 2.367457944102682 1110 28-29 1.2256370091896471 1110 30-31 -2.158470988076253 1110 32-33 1.8773586428191607 1110 34-35 2.891306390977448 1110 36-37 1.431705209994682 1110 38-39 0.8928661616161655 1110 40-41 -1.07992851446798 1110 42-43 -1.001238892686267 1110 44-45 -0.1633890597706369 1110 46-47 2.8041291676160043 1110 48-49 1.4330665679349934 1110 50-51 -1.8247389306599828 1110 52-53 -1.7458580162527468 1110 54-55 -0.5507684932026962 1110 56-57 0.8345171641224312 1110 58-59 -1.166597839295214 1110 60-61 0.7624781651097408 1110 62-63 2.0207825434799105 1110 64-65 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1.7895629224576624 2117 2 0.9267866636287749 2117 3 1.041435976304399 2117 4 0.48473551302498663 2117 5 0.5988826232247249 2117 6 0.7756227690438138 2117 7 1.3428182197919014 2117 8 1.2059894053315077 2117 9 1.4060530113161676 2117 10-11 0.9350127211969266 2117 12-13 1.0627829042302679 2117 14-15 -1.5874302232854802 2117 16-17 0.6182639363560369 2117 18-19 -0.5862796195033084 2117 20-21 1.2081809637730672 2117 22-23 0.6863736044657074 2117 24-25 0.8337752525252569 2117 26-27 0.5341246107693536 2117 28-29 2.614525898078533 2117 30-31 3.008195678590411 2117 32-33 2.3773586428191607 2117 34-35 4.780195279866334 2117 36-37 -1.0405170122275464 2117 38-39 -2.94046717171717 2117 40-41 3.1422937077542414 2117 42-43 -0.44568333713070984 2117 44-45 2.5588331624515845 2117 46-47 -4.140315276828439 2117 48-49 3.266399901268322 2117 50-51 2.9530388471177957 2117 52-53 2.893030872636139 2117 54-55 2.5881203956861896 2117 56-57 2.778961608566874 2117 58-59 2.8611799384825645 2117 60-61 2.623589276220855 2117 62-63 -3.1736619009645324 2117 64-65 -7.953502126528434 2117 66-67 -4.836293384977601 2117 68-69 5.710651154401148 2117 70-71 -4.142146084909243 2117 72-73 -2.1334923482949826 2117 74-75 3.794064707222603 2117 76 12.322428229665071 2118 1 1.7895629224576624 2118 2 0.9267866636287749 2118 3 1.041435976304399 2118 4 0.48473551302498663 2118 5 0.5988826232247249 2118 6 -0.5577105642895219 2118 7 -0.9905151135414343 2118 8 0.872656071998172 2118 9 -0.5939469886838324 2118 10-11 0.5183460545302623 2118 12-13 -5.187217095769732 2118 14-15 0.9125697767145198 2118 16-17 -3.5484027303106274 2118 18-19 0.7470537138300202 2118 20-21 -4.8751523695602685 2118 22-23 1.4363736044657074 2118 24-25 0.8337752525252569 2118 26-27 -2.6325420558973107 2118 28-29 -0.21880743525479573 2118 30-31 2.1748623452570826 2118 32-33 2.8773586428191607 2118 34-35 3.6135286131996622 2118 36-37 -3.2905170122275464 2118 38-39 4.892866161616158 2118 40-41 -2.024372958912423 2118 42-43 1.5543166628692902 2118 44-45 1.725499829118256 2118 46-47 -4.973648610161774 2118 48-49 -1.4002667653983423 2118 50-51 -2.88029448621554 2118 52-53 -5.690302460697197 2118 54-55 1.004787062352861 2118 56-57 -1.221038391433126 2118 58-59 -0.7221533948507712 2118 60-61 -1.626410723779145 2118 62-63 -3.5903285676311967 2118 64-65 0.29649787347156575 2118 66-67 -3.169626718310937 2118 68-69 -7.539348845598852 2118 70-71 -11.642146084909243 2118 72-73 -5.30015901496165 2118 74-75 -1.9559352927773972 2118 76 -0.010905103668260807 2119 1 1.7895629224576624 2119 2 0.9267866636287749 2119 3 1.041435976304399 2119 4 0.48473551302498663 2119 5 0.5988826232247249 2119 6 0.7756227690438138 2119 7 -0.1571817802080986 2119 8 1.2059894053315077 2119 9 1.4060530113161676 2119 10-11 1.4350127211969266 2119 12-13 -1.1872170957697321 2119 14-15 1.9125697767145198 2119 16-17 1.618263936356037 2119 18-19 1.9137203804966916 2119 20-21 1.9581809637730672 2119 22-23 -2.5636263955342926 2119 24-25 3.083775252525257 2119 26-27 3.5341246107693536 2119 28-29 3.114525898078533 2119 30-31 3.008195678590411 2119 32-33 2.6273586428191607 2119 34-35 -1.7198047201336664 2119 36-37 3.7094829877724536 2119 38-39 6.30953282828283 2119 40-41 1.8922937077542414 2119 42-43 2.80431666286929 2119 44-45 1.0588331624515845 2119 46-47 3.1096847231715614 2119 48-49 1.016399901268322 2119 50-51 -6.546961152882204 2119 52-53 1.893030872636139 2119 54-55 2.8381203956861896 2119 56-57 3.278961608566874 2119 58-59 4.1111799384825645 2119 60-61 3.873589276220855 2119 62-63 2.5763380990354676 2119 64-65 3.0464978734715658 2119 66-67 -2.5862933849776013 2119 68-69 -9.789348845598852 2119 70-71 1.3578539150907574 2119 72-73 5.866507651705017 2119 74-75 10.044064707222603 2119 76 13.822428229665071 >>END_MODULE >>Per sequence quality scores pass #Quality Count 23 4.0 24 8.0 25 5.0 26 32.0 27 53.0 28 88.0 29 144.0 30 219.0 31 291.0 32 402.0 33 509.0 34 609.0 35 367.0 36 84.0 37 45.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 2.272727272727273 28.601398601398603 11.363636363636363 57.76223776223777 2 1.6433566433566433 4.755244755244755 84.26573426573427 9.335664335664335 3 1.5734265734265735 29.510489510489514 61.81818181818181 7.097902097902098 4 1.118881118881119 49.72027972027972 20.874125874125873 28.28671328671329 5 2.097902097902098 40.349650349650354 52.55244755244755 5.0 6 1.7482517482517483 55.6993006993007 9.090909090909092 33.46153846153846 7 2.937062937062937 40.03496503496503 7.6223776223776225 49.40559440559441 8 1.7132867132867133 55.24475524475524 27.517482517482517 15.524475524475525 9 2.097902097902098 14.58041958041958 5.174825174825175 78.14685314685315 10-11 2.36013986013986 30.122377622377623 41.45104895104895 26.066433566433567 12-13 14.632867132867133 5.5419580419580425 33.82867132867133 45.9965034965035 14-15 19.265734265734267 16.1013986013986 7.8671328671328675 56.76573426573427 16-17 31.888111888111887 28.53146853146853 2.097902097902098 37.48251748251748 18-19 30.244755244755243 21.363636363636363 1.9230769230769231 46.468531468531474 20-21 17.727272727272727 8.146853146853147 2.395104895104895 71.73076923076923 22-23 19.073426573426573 41.78321678321678 2.5524475524475525 36.59090909090909 24-25 32.010489510489506 43.51398601398601 2.902097902097902 21.573426573426573 26-27 43.33916083916084 34.00349650349651 14.982517482517482 7.674825174825174 28-29 29.755244755244753 21.503496503496503 20.89160839160839 27.84965034965035 30-31 8.391608391608392 20.75174825174825 52.46503496503496 18.39160839160839 32-33 14.423076923076922 65.85664335664335 11.765734265734265 7.954545454545454 34-35 7.045454545454545 49.80769230769231 14.527972027972028 28.618881118881117 36-37 27.02797202797203 34.03846153846154 19.65034965034965 19.283216783216783 38-39 17.23776223776224 11.975524475524475 29.318181818181817 41.46853146853147 40-41 7.255244755244755 16.486013986013987 43.11188811188811 33.14685314685315 42-43 39.44055944055944 6.7482517482517475 44.26573426573427 9.545454545454547 44-45 9.527972027972027 27.01048951048951 34.42307692307692 29.03846153846154 46-47 40.38461538461539 5.1923076923076925 21.486013986013987 32.93706293706294 48-49 29.86013986013986 2.8146853146853146 20.367132867132867 46.95804195804196 50-51 8.059440559440558 3.146853146853147 46.41608391608392 42.37762237762237 52-53 13.863636363636363 3.3741258741258737 68.88111888111888 13.881118881118882 54-55 6.538461538461539 3.8111888111888113 48.65384615384615 40.99650349650349 56-57 38.53146853146853 4.685314685314685 26.328671328671327 30.454545454545457 58-59 8.531468531468532 29.510489510489514 52.569930069930074 9.388111888111888 60-61 26.17132867132867 58.75874125874125 10.821678321678322 4.248251748251748 62-63 4.073426573426573 89.33566433566433 2.4825174825174825 4.108391608391608 64-65 1.6958041958041958 91.87062937062937 2.0454545454545454 4.388111888111888 66-67 1.7307692307692308 89.84265734265733 1.993006993006993 6.433566433566433 68-69 2.1153846153846154 82.15034965034965 3.8636363636363633 11.87062937062937 70-71 3.146853146853147 73.04195804195804 5.944055944055944 17.867132867132867 72-73 4.090909090909091 59.23076923076923 9.685314685314687 26.99300699300699 74-75 5.909090909090909 57.84965034965035 9.388111888111888 26.853146853146853 76 4.405594405594406 60.10489510489511 8.286713286713287 27.202797202797203 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.5 25 1.0 26 2.0 27 2.5 28 2.0 29 2.0 30 3.0 31 3.5 32 5.5 33 8.0 34 8.5 35 8.0 36 14.5 37 22.0 38 65.0 39 235.5 40 502.0 41 623.0 42 605.0 43 541.5 44 372.5 45 193.5 46 120.0 47 87.5 48 51.0 49 37.0 50 27.0 51 17.5 52 10.0 53 9.5 54 7.0 55 8.0 56 7.0 57 7.0 58 9.0 59 8.5 60 7.5 61 7.0 62 7.0 63 6.5 64 5.5 65 4.5 66 2.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 2860.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.46153846153846 #Duplication Level Percentage of deduplicated Percentage of total 1 81.57248157248156 23.216783216783217 2 8.722358722358722 4.965034965034965 3 2.334152334152334 1.993006993006993 4 1.71990171990172 1.9580419580419581 5 0.9828009828009828 1.3986013986013985 6 0.6142506142506142 1.048951048951049 7 0.85995085995086 1.7132867132867133 8 0.12285012285012285 0.27972027972027974 9 0.12285012285012285 0.3146853146853147 >10 2.211302211302211 12.587412587412588 >50 0.4914004914004914 9.300699300699302 >100 0.12285012285012285 16.853146853146853 >500 0.12285012285012285 24.37062937062937 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT 697 24.37062937062937 No Hit ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT 482 16.853146853146853 Illumina PCR Primer Index 9 (95% over 22bp) CTTTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC 84 2.937062937062937 No Hit TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG 70 2.4475524475524475 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC 60 2.097902097902098 No Hit TATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCT 52 1.8181818181818181 Illumina PCR Primer Index 9 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGT 40 1.3986013986013985 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGT 39 1.3636363636363635 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCGT 39 1.3636363636363635 No Hit CTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC 34 1.1888111888111887 No Hit ATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGTCTT 23 0.8041958041958042 No Hit TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCG 19 0.6643356643356644 No Hit TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC 18 0.6293706293706294 No Hit ATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCGTCTT 17 0.5944055944055944 No Hit CTTATACACATCTCCGAGCCCACGCGACGTTAACAGATCTCGTATGCCGT 17 0.5944055944055944 No Hit ATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGTCTT 17 0.5944055944055944 No Hit ACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTTCT 15 0.5244755244755245 Illumina PCR Primer Index 9 (95% over 24bp) TTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC 14 0.48951048951048953 No Hit ATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTTCTGCTT 12 0.4195804195804196 Illumina PCR Primer Index 9 (96% over 28bp) CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCAGT 12 0.4195804195804196 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCC 12 0.4195804195804196 No Hit CTTATACACATCTCCGAGCCCAAGAGACGTTAACAGATCTCGTATGCCGT 11 0.38461538461538464 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTAGTATGCCGT 11 0.38461538461538464 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTAACAGCTCTCGTATGCCGT 10 0.34965034965034963 No Hit TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCG 9 0.3146853146853147 No Hit CTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG 8 0.27972027972027974 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTAAAAGATCTCGTATGCCGT 7 0.24475524475524477 No Hit TCTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC 7 0.24475524475524477 No Hit ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTCTGCCGTCTT 7 0.24475524475524477 No Hit CTTATACACATATCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT 7 0.24475524475524477 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCCCGTATGCCGT 7 0.24475524475524477 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGC 7 0.24475524475524477 No Hit CTTATAACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG 7 0.24475524475524477 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTAACAGACATCGTATGCCGT 6 0.2097902097902098 No Hit ATACACATCTCCGAGCCCACGAGACGTTCACAGATCTCGTATGCCGTCTT 6 0.2097902097902098 TruSeq Adapter, Index 9 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCACGTATGCCGT 6 0.2097902097902098 No Hit CTTATTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG 6 0.2097902097902098 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCC 6 0.2097902097902098 No Hit CTTATACACATCTCCGAGCCCACGAGCCGTTAACAGATCTCGTATGCCGT 5 0.17482517482517482 No Hit ATACACATCTCCGAGCCCACGAGACGTTAACAGAGCTCGTATGCCGTCTT 5 0.17482517482517482 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAAATCGTATGCCGT 5 0.17482517482517482 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAGCTCGTATGCCGT 5 0.17482517482517482 No Hit ATACACATCTCCGAGCCCACGAGACGTTAACAGCTCTCGTATGCCGTCTT 5 0.17482517482517482 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGACGT 5 0.17482517482517482 No Hit CTTATCACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC 5 0.17482517482517482 No Hit CTTATACACATCTCCGAGCCAACGAGACGTTAACAGATCTCGTATGCCGT 5 0.17482517482517482 No Hit ATTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT 4 0.13986013986013987 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTACCAGATCTCGTATGCCGT 4 0.13986013986013987 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTCACAGATCTCGTATGCCGT 4 0.13986013986013987 No Hit TATCACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT 4 0.13986013986013987 Illumina PCR Primer Index 9 (95% over 22bp) CTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTA 4 0.13986013986013987 No Hit ATACACATCTCCGAGCCCACGAGACGTTACCAGATCTCGTATGCCGTCTT 4 0.13986013986013987 Illumina PCR Primer Index 9 (95% over 22bp) CTTATACACATCTCCGAGCCCACGTGACGTTAACAGATCTCGTATGCCGT 4 0.13986013986013987 No Hit CTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTTCTGCTTGA 4 0.13986013986013987 Illumina PCR Primer Index 9 (96% over 29bp) CTTATACACATCTACGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT 4 0.13986013986013987 No Hit CTTATACACATCTCCGAGCCCACGAGACGCTAACAGATCTCGTATGCCGT 4 0.13986013986013987 No Hit ATACACATCTACGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT 4 0.13986013986013987 Illumina PCR Primer Index 9 (95% over 22bp) CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGA 4 0.13986013986013987 No Hit ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCCT 4 0.13986013986013987 No Hit ATACACATCTCCGAGCACACGAGACGTTAACAGATCTCGTATGCCGTCTT 4 0.13986013986013987 TruSeq Adapter, Index 9 (95% over 22bp) CTTATACACATCTGACGCCTGTCTCTTATACACATCTCCGAGCCCACGAG 3 0.1048951048951049 No Hit CTTATACACATCTCCGAGACCACGAGACGTTAACAGATATCGTATGCCGT 3 0.1048951048951049 No Hit AGTAATAATCCTTCCCACTGTGAACATATGTTTCCTTTTGCTGTCTCTTA 3 0.1048951048951049 No Hit CTTATACACATCTCCGAGCACACGAGACGTTAACAGATCTCGTATGCCGT 3 0.1048951048951049 No Hit TACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTTC 3 0.1048951048951049 Illumina PCR Primer Index 9 (95% over 23bp) CTTATACACATCTCCGAGCCCACGAGACATTAACAGATCTCGTATGCCGT 3 0.1048951048951049 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTCTGCCGT 3 0.1048951048951049 No Hit TATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCGTCT 3 0.1048951048951049 No Hit CTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG 3 0.1048951048951049 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCC 3 0.1048951048951049 No Hit CTTAACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC 3 0.1048951048951049 No Hit TATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGTCT 3 0.1048951048951049 No Hit ATACACATCTCCGAGCCCACGAGCCGTTAACAGATCTCGTATGCCGTCTT 3 0.1048951048951049 Illumina PCR Primer Index 9 (95% over 22bp) TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCG 3 0.1048951048951049 No Hit CTTTACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGTC 3 0.1048951048951049 No Hit CTTATACACATCTCCGAGCCCACGAGAAGTTAACAGATCTCGTATGCCGT 3 0.1048951048951049 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGACATCGTATGCC 3 0.1048951048951049 No Hit TGTAAAGAATTTGGGTGTGAAATACACATAAGCAAAATTTCATCCACTGG 3 0.1048951048951049 No Hit ATACACCTCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT 3 0.1048951048951049 Illumina PCR Primer Index 9 (95% over 22bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.13986013986013987 0.0 2 0.0 0.0 0.0 0.13986013986013987 0.0 3 0.0 0.0 0.0 0.13986013986013987 0.0 4 0.0 0.0 0.0 0.13986013986013987 0.0 5 0.0 0.0 0.0 0.13986013986013987 0.0 6 0.0 0.0 0.0 0.13986013986013987 0.0 7 0.0 0.0 0.0 0.13986013986013987 0.0 8 0.0 0.0 0.0 0.13986013986013987 0.0 9 0.0 0.0 0.0 0.13986013986013987 0.0 10 0.0 0.0 0.0 0.13986013986013987 0.0 11 0.0 0.0 0.0 0.13986013986013987 0.0 12 0.0 0.0 0.0 0.13986013986013987 0.0 13 0.0 0.0 0.0 0.13986013986013987 0.0 14 0.0 0.0 0.0 0.13986013986013987 0.0 15 0.0 0.0 0.0 0.13986013986013987 0.0 16 0.0 0.0 0.0 0.13986013986013987 0.0 17 0.0 0.0 0.0 0.2097902097902098 0.0 18 0.0 0.0 0.0 0.27972027972027974 0.0 19 0.0 0.0 0.0 0.5244755244755245 0.0 20 0.0 0.0 0.0 0.6293706293706294 0.0 21 0.0 0.0 0.0 0.6293706293706294 0.0 22 0.0 0.0 0.0 0.6293706293706294 0.0 23 0.0 0.0 0.0 0.6643356643356644 0.0 24 0.0 0.0 0.0 0.6643356643356644 0.0 25 0.0 0.0 0.0 0.6643356643356644 0.0 26 0.0 0.0 0.0 0.6643356643356644 0.0 27 0.0 0.0 0.0 0.6643356643356644 0.0 28 0.0 0.0 0.0 0.6993006993006993 0.0 29 0.0 0.0 0.0 0.7342657342657343 0.0 30 0.0 0.0 0.0 0.7342657342657343 0.0 31 0.0 0.0 0.0 0.8041958041958042 0.0 32 0.0 0.0 0.0 0.8391608391608392 0.0 33 0.0 0.0 0.0 0.8741258741258742 0.0 34 0.0 0.0 0.0 0.9090909090909091 0.0 35 0.0 0.0 0.0 1.013986013986014 0.0 36 0.0 0.0 0.0 1.083916083916084 0.0 37 0.0 0.0 0.0 1.083916083916084 0.0 38 0.0 0.0 0.0 1.1888111888111887 0.0 39 0.0 0.0 0.0 1.2237762237762237 0.0 40 0.0 0.0 0.0 1.2937062937062938 0.0 41 0.0 0.0 0.0 1.6083916083916083 0.0 42 0.0 0.0 0.0 1.7482517482517483 0.0 43 0.0 0.0 0.0 1.8881118881118881 0.0 44 0.0 0.0 0.0 2.097902097902098 0.0 45 0.0 0.0 0.0 2.3076923076923075 0.0 46 0.0 0.0 0.0 2.4125874125874125 0.0 47 0.0 0.0 0.0 2.4825174825174825 0.0 48 0.0 0.0 0.0 2.5174825174825175 0.0 49 0.0 0.0 0.0 2.5174825174825175 0.0 50 0.0 0.0 0.0 2.5524475524475525 0.0 51 0.0 0.0 0.0 2.6223776223776225 0.0 52 0.0 0.0 0.0 2.6223776223776225 0.0 53 0.0 0.0 0.0 2.867132867132867 0.0 54 0.0 0.0 0.0 2.902097902097902 0.0 55 0.0 0.0 0.0 3.041958041958042 0.0 56 0.0 0.0 0.0 3.1818181818181817 0.0 57 0.0 0.0 0.0 3.1818181818181817 0.0 58 0.0 0.0 0.0 3.3216783216783217 0.0 59 0.0 0.0 0.0 3.3916083916083917 0.0 60 0.0 0.0 0.0 3.4615384615384617 0.0 61 0.0 0.0 0.0 3.4615384615384617 0.0 62 0.0 0.0 0.0 3.4965034965034967 0.0 63 0.0 0.0 0.0 3.5664335664335662 0.0 64 0.0 0.0 0.0 3.5664335664335662 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTACA 15 0.0020034623 70.0 1 ACAGACC 15 0.0020034623 70.0 33 CCTCGTA 15 0.0020034623 70.0 38 TTACACA 15 0.0020034623 70.0 3 ACCTCGT 15 0.0020034623 70.0 37 CAGACCT 15 0.0020034623 70.0 34 AGACCTC 15 0.0020034623 70.0 35 AACAGAC 15 0.0020034623 70.0 32 GACCTCG 15 0.0020034623 70.0 36 TTTACAC 15 0.0020034623 70.0 2 CTTATAC 135 0.0 44.074074 1 TTATACA 150 0.0 42.0 2 TCTTATA 50 2.4111068E-6 42.0 1 TATACAC 165 0.0 40.303032 3 ATACACA 225 0.0 29.555555 4 AGACGTT 255 0.0 28.823528 25 CGTATGC 255 0.0 28.823528 41 TCGTATG 255 0.0 28.823528 40 ATCTCCG 270 0.0 28.518518 10 CATCTCC 270 0.0 28.518518 9 >>END_MODULE