FastQCFastQC Report
Wed 25 May 2016
SRR1780587_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780587_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51577
Sequences flagged as poor quality0
Sequence length50
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGAATGATCGTGTAGATC1240.24041724024274386No Hit
CTTATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTG640.124086317544642No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGC207.731667E-444.06316819
GTCTCTC207.8061E-443.9776943
TCTGACC301.2877672E-436.64807511
TCCTCGG250.002315628635.25053423
GACGAAT250.002315628635.25053423
CCATGCC250.002337835535.1821564
ATGCCCA250.002337835535.1821566
CGTTTAG250.002337835535.18215632
GCTCCTC353.1489556E-431.47369421
ATACCCA353.1851066E-431.4126364
CTTTACA502.2783443E-630.8442171
CCCAATG502.3132307E-630.78438627
TTTACAC651.6876584E-830.4460952
CTATACA300.005654902729.3754441
CTGGACC300.00568186329.34692613
CCAATGT454.009206E-529.31846228
TGGCACA300.00570892529.3184635
AACGGCT300.00570892529.3184626
TCCACAT300.00570892529.318465
CAGTGTA300.00570892529.3184634
Produced by FastQC (version 0.11.5)