FastQCFastQC Report
Wed 25 May 2016
SRR1780587_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780587_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51577
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT2511948.701940787560346No Hit
ATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCTT556210.783876534114043RNA PCR Primer, Index 14 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG37387.2474164840917465No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC33356.466060453302829No Hit
CTTTACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC7841.5200573899218643No Hit
TATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCT6761.3106617290652811No Hit
CTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC4870.9442193225662601No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC2770.5370610931229036No Hit
ATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCTTCTGCTT2140.4149136242898967RNA PCR Primer, Index 27 (96% over 28bp)
CATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCTTCTGCT1960.3800143474804661RNA PCR Primer, Index 14 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC1870.36256470907575084No Hit
ACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCTTCT1660.32184888613141516RNA PCR Primer, Index 14 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATATCGTATGCCGT1630.31603233999651004No Hit
CTTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG1430.2772553657638095No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTA1410.2733776683405394No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC960.18612947631696297No Hit
TCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCTTCTGCTTG890.17255753533551776RNA PCR Primer, Index 14 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCATCTCGTATGCCGTC770.1492913507958974No Hit
CTTATAACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG700.13571940981445219No Hit
CTTAACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC670.12990286367954706No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGC660.12796401496791204No Hit
CTTATTACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG550.10663667913992671No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCAG207.786627E-444.00000431
GGTTCCA207.786627E-444.00000430
TCTTTAT408.050847E-944.0000041
CTTTACA950.044.01
TTACACA950.044.03
TTTACAC950.044.02
CTATACA750.038.1333351
CCGTCTT7000.038.02857244
TCATGTG250.002332026635.22
GTGGCCG250.002332026635.24
GCCGTCT7950.033.48427643
CTTATAC32950.032.6494671
TTATACA33300.032.3063052
ATGCCGT34300.031.10787244
TATACAC34900.030.8252143
CTCTTAT4250.030.0235311
CTTTATA602.8540308E-729.3333362
TCTGCTT300.005694791729.33333644
TATGCCG39150.027.25415243
TTTATAC655.7481884E-727.0769233
Produced by FastQC (version 0.11.5)