FastQCFastQC Report
Wed 25 May 2016
SRR1780586_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780586_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11740
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT3533.0068143100511073RNA PCR Primer, Index 42 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT1431.2180579216354344No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC620.5281090289608177No Hit
CTTTACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC390.3321976149914821No Hit
TATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCT240.20442930153321978RNA PCR Primer, Index 42 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCGTCTT180.15332197614991483No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGT160.1362862010221465No Hit
CCTTTTACCCCTACCATGAGCCCTACAAACAACTAACCTGCCACTAATAG140.1192504258943782No Hit
CTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC140.1192504258943782RNA PCR Primer, Index 32 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTTCT120.10221465076660989RNA PCR Primer, Index 42 (95% over 24bp)
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA120.10221465076660989No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGCGC150.00217011970.013
TAGCGGA150.00217011970.034
GAGTGAT150.00217011970.065
AATTTAC150.00217011970.053
GGCGTGA150.00217011970.024
GGCGCCA150.00217011970.015
CGCCATT150.00217011970.017
TGATTCA150.00217011970.041
ATTGGCG150.00217011970.021
AGGCGCC150.00217011970.014
GATGATT150.00217011970.039
TATTGAG150.00217011970.09
TTGAGGC150.00217011970.011
GCGTGAA150.00217011970.025
TACGTCT150.00217011970.057
TCTCGAG150.00217011970.061
ATAAAGA150.00217011970.01
GTCTCGA150.00217011970.060
GCCATTG150.00217011970.018
GATTCAG150.00217011970.042
Produced by FastQC (version 0.11.5)