FastQCFastQC Report
Wed 25 May 2016
SRR1780585_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780585_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences300313
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCGTCCTTTAGTGTTGTGTATGGTTATTATTTGTTTTGAGGTTAGTTT4440.14784574760333385No Hit
ATAATAAAGGTGGATGCGACAATGGATTTTACATAATGGGGGTATGAGTT3810.1268676347677257No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTTG207.747202E-444.11464721
CGACTTC207.759985E-444.09988416
ACTTCGC207.772785E-444.08513318
GACTTCG207.785602E-444.0703917
GAGGTAC207.8370364E-444.01152411
GCGACTT250.002323973235.27990715
CGACGTT250.002329703235.26220714
TCACCTA250.002335444335.24452624
AGCGCGT250.002348883835.2033425
GTATAGC250.002366253235.1505241
TTTAGAG451.0547756E-634.1342933
CGTTTAC401.7937173E-533.0583214
CCGTTTA401.7957715E-533.0527913
CGCTTGT401.810207E-533.01415322
CTAGATC353.1634656E-431.51046220
ACGTCAA353.1759107E-431.4893818
ACGAGAG353.1883962E-431.46832724
CGGCCCA353.2103434E-431.43155525
ATTCCAA353.238744E-431.38441
CAATTTT353.2546115E-431.3582639
Produced by FastQC (version 0.11.5)