FastQCFastQC Report
Wed 25 May 2016
SRR1780585_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780585_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences300313
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGT52581.7508399569782194No Hit
ATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGTCTT22730.7568769916720222TruSeq Adapter, Index 3 (95% over 21bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC10800.3596247914675688No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCC8790.29269462194443796No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGG6540.21777279038869443No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCG5260.17515059288142704No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCCT4770.15883428289817622No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATTCCGT4760.15850129698015072No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCCTATGCCGT4610.1535065082097678No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGC3320.11055132478447487No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTTCG207.832417E-444.01682313
GCTTTAC207.8388466E-444.0094916
GTACCTC207.851718E-443.9948427
GTCGAAA207.851718E-443.9948435
CGAAATC207.851718E-443.9948437
GAGTCGA207.851718E-443.9948433
CAAGCGA207.851718E-443.9948442
TCGAAAT207.851718E-443.9948436
ATTCCCT404.1147723E-738.49548344
AACTCGG301.2997037E-436.6623643
ACAAGCG301.2997037E-436.6623641
CCGTCTT3850.036.5671444
GCCGTCT3200.035.74580843
ACAGGAT250.002345582535.2134610
ATTGCGA250.002345582535.2134615
ATGCCAT250.002345582535.2134610
TTGCGAT250.002347500535.20759216
TTGAGCA250.002349419835.201734
GGAATTT250.002351340435.1958728
GGTACAA250.002351340435.1958730
Produced by FastQC (version 0.11.5)