FastQCFastQC Report
Wed 25 May 2016
SRR1780584_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780584_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55567
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACAAG150.002214874469.99145
GTCAGTA207.867497E-569.99156
TCAATGT150.002214874469.99153
AATTCAG207.867497E-569.99118
CCGACCA207.867497E-569.99138
AATAACA207.867497E-569.99128
CGACCAC252.372721E-455.992839
TTCGTCC252.372721E-455.99282
GATAGTC252.372721E-455.992852
GTTCGTC200.006677531652.9700281
TGGCGCA200.00692095352.49324854
TTGTAAA200.00692095352.49324859
CAATGTT200.00692095352.49324854
CAAAATT352.0632504E-549.9935715
ACACCCG305.834651E-446.66066434
ATATCCA305.834651E-446.6606649
ATTCAGA305.834651E-446.66066419
GAGGTAT305.834651E-446.66066422
TCAGAAT305.834651E-446.66066421
ACCCGAC305.834651E-446.66066436
Produced by FastQC (version 0.11.5)