##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780584_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 55567 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.226429355552757 34.0 31.0 34.0 12.0 34.0 2 31.7470261126208 34.0 34.0 34.0 23.0 34.0 3 31.81319848111289 34.0 34.0 34.0 23.0 34.0 4 32.02172152536578 34.0 34.0 34.0 27.0 34.0 5 31.967120773120737 34.0 34.0 34.0 27.0 34.0 6 34.65803444490435 38.0 36.0 38.0 24.0 38.0 7 34.47549804740223 38.0 36.0 38.0 24.0 38.0 8 33.41598430723271 37.0 34.0 38.0 21.0 38.0 9 34.2882646174888 38.0 35.0 38.0 24.0 38.0 10-11 34.515287850702755 38.0 36.0 38.0 25.0 38.0 12-13 34.72071553260028 38.0 36.0 38.0 25.5 38.0 14-15 34.58735400507496 38.0 36.0 38.0 25.0 38.0 16-17 34.424379577806974 38.0 36.0 38.0 24.0 38.0 18-19 34.40750805334101 38.0 35.5 38.0 24.0 38.0 20-21 34.456655928878654 38.0 36.0 38.0 24.0 38.0 22-23 34.40132632677668 38.0 35.5 38.0 24.0 38.0 24-25 34.766336134756244 38.0 36.5 38.0 26.0 38.0 26-27 34.719500782838736 38.0 36.0 38.0 25.0 38.0 28-29 34.89435276333076 38.0 36.5 38.0 26.0 38.0 30-31 34.84356722515162 38.0 36.5 38.0 26.0 38.0 32-33 34.38479673187324 38.0 35.5 38.0 24.0 38.0 34-35 34.58917163064409 38.0 36.0 38.0 25.0 38.0 36-37 34.57056346392643 38.0 36.0 38.0 24.5 38.0 38-39 34.92123022657333 38.0 36.5 38.0 26.0 38.0 40-41 34.53933089783504 38.0 36.0 38.0 24.0 38.0 42-43 34.75079633595479 38.0 36.0 38.0 26.0 38.0 44-45 33.96479925135422 37.5 34.0 38.0 22.5 38.0 46-47 34.606619036478484 38.0 36.0 38.0 25.0 38.0 48-49 34.45326362769269 38.0 35.5 38.0 24.0 38.0 50-51 34.28830960822071 38.0 35.0 38.0 24.0 38.0 52-53 34.30462324761099 38.0 35.0 38.0 24.0 38.0 54-55 34.090341389673725 37.5 34.0 38.0 23.0 38.0 56-57 34.1035866611478 37.5 34.5 38.0 23.5 38.0 58-59 34.05945975129123 37.5 34.5 38.0 23.0 38.0 60-61 34.11854158043443 37.5 34.5 38.0 23.5 38.0 62-63 34.100779239476665 37.0 34.5 38.0 23.0 38.0 64-65 34.19529576907157 37.5 35.0 38.0 23.5 38.0 66-67 34.132011805568055 38.0 34.5 38.0 23.5 38.0 68-69 33.549462810661 37.0 34.0 38.0 21.0 38.0 70-71 33.71039465870031 37.0 34.0 38.0 22.0 38.0 72-73 33.841767595875254 37.0 34.0 38.0 23.0 38.0 74-75 33.36312019723937 37.0 33.0 38.0 21.5 38.0 76 32.792934655460975 37.0 31.0 38.0 20.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.152258275694127 1101 2 0.04667845776646118 1101 3 0.42269507901914594 1101 4 -1.696998745735069 1101 5 -0.2565070399678042 1101 6 -0.3421894591566428 1101 7 -0.5711127500528406 1101 8 -0.13986498788913337 1101 9 -0.40874752934360004 1101 10-11 -0.70530246359953 1101 12-13 -0.7656765182615288 1101 14-15 -0.5421518545014337 1101 16-17 -1.4495519458054247 1101 18-19 -0.09046393745344261 1101 20-21 -1.2319809009372023 1101 22-23 -0.76654856888387 1101 24-25 -0.851979428086274 1101 26-27 -1.1141180299422686 1101 28-29 -0.540329835422952 1101 30-31 -0.4922717038818334 1101 32-33 0.5635725573337425 1101 34-35 -1.0267845183149191 1101 36-37 -0.27728020040765955 1101 38-39 -0.27556110123024524 1101 40-41 0.13005547615070157 1101 42-43 -0.06045275215502244 1101 44-45 0.4345175022143195 1101 46-47 0.19985604825574654 1101 48-49 -0.9020878147180227 1101 50-51 -0.8260511813564619 1101 52-53 -0.1006104314712033 1101 54-55 -1.079106598112645 1101 56-57 -1.2176367360982496 1101 58-59 0.041323312630495934 1101 60-61 -0.37756631321914824 1101 62-63 -1.1619277031364348 1101 64-65 0.0015815471225693045 1101 66-67 -1.0014272684229653 1101 68-69 0.42879747662617973 1101 70-71 0.0936336366348769 1101 72-73 -0.6097719592037691 1101 74-75 -1.4572849618443158 1101 76 -1.387953495460689 1102 1 0.3659060395537992 1102 2 -0.20137254750450495 1102 3 0.10877278630696807 1102 4 0.3508186957950912 1102 5 0.07237595722311951 1102 6 0.21444974798914274 1102 7 1.514011764671146 1102 8 1.5272435561250077 1102 9 0.6817742015530968 1102 10-11 -0.09488047073269001 1102 12-13 -3.0249164892238696 1102 14-15 -1.2079978918084961 1102 16-17 -1.8322966353865908 1102 18-19 -0.6113584234553997 1102 20-21 0.695314494069585 1102 22-23 -0.5341526467805409 1102 24-25 0.3173227213314007 1102 26-27 -4.908929127376233 1102 28-29 -0.2590749037561295 1102 30-31 0.09747674165678433 1102 32-33 -6.737787793643122 1102 34-35 -1.144418580745878 1102 36-37 0.2977758233274699 1102 38-39 0.41334146481463563 1102 40-41 0.7323658634246328 1102 42-43 0.4548235790004824 1102 44-45 -5.034472176793344 1102 46-47 -0.21419880766067934 1102 48-49 0.6114052257137601 1102 50-51 -5.7087958709376245 1102 52-53 -1.20049049333403 1102 54-55 0.35642665315775446 1102 56-57 -1.5627429444749836 1102 58-59 0.33078453789325835 1102 60-61 0.27890561178796247 1102 62-63 -4.501542007337431 1102 64-65 -0.38804380401211347 1102 66-67 -0.10607329310496993 1102 68-69 -7.017877396334079 1102 70-71 -0.40597183001916193 1102 72-73 0.44074409041274265 1102 74-75 -6.066854762683885 1102 76 -1.4202421044939264 1103 1 -0.21233187751862204 1103 2 0.08464559610679245 1103 3 0.16151226660013762 1103 4 0.4404604363516995 1103 5 0.4629248783445341 1103 6 -0.9174758324526593 1103 7 0.8181735195786288 1103 8 2.048001077523189 1103 9 0.5461583950849445 1103 10-11 0.6935209438539545 1103 12-13 -1.146553001960271 1103 14-15 -0.6881244870624599 1103 16-17 -0.36575212203514695 1103 18-19 0.1381034185824248 1103 20-21 -0.11977699278399001 1103 22-23 0.09940022083259947 1103 24-25 0.8968932200244453 1103 26-27 0.11267407081965786 1103 28-29 0.9851975579321532 1103 30-31 0.7993884837509881 1103 32-33 -4.566435735829579 1103 34-35 -0.25686796827086056 1103 36-37 0.26592199728062127 1103 38-39 1.02571397263074 1103 40-41 0.7570186234061822 1103 42-43 0.873292647730942 1103 44-45 -4.244187209339088 1103 46-47 0.4469923152955957 1103 48-49 0.8835209553021954 1103 50-51 0.893069743331246 1103 52-53 0.5037020134570156 1103 54-55 0.9469909502209504 1103 56-57 1.202697063725516 1103 58-59 0.9182864598859766 1103 60-61 1.0463957455586552 1103 62-63 0.765986566287701 1103 64-65 1.0154337214863247 1103 66-67 0.29789113721638927 1103 68-69 -2.97979631386778 1103 70-71 0.7894948298137479 1103 72-73 1.4549924631255777 1103 74-75 -1.3234151645084964 1103 76 0.006619665789500573 1104 1 -1.7173103548437219 1104 2 -0.2036495471603068 1104 3 0.3693039097150539 1104 4 0.5518565840102951 1104 5 0.5955828199941742 1104 6 0.13104801641033248 1104 7 0.9361569101608325 1104 8 1.1824634454522354 1104 9 0.6455540209817272 1104 10-11 0.10282134821935784 1104 12-13 -0.32665865872796473 1104 14-15 0.01391690604159379 1104 16-17 -0.4220263078693094 1104 18-19 0.3475364374093317 1104 20-21 1.3948432080943078 1104 22-23 0.5968675020187391 1104 24-25 0.5944018073543234 1104 26-27 0.7242223990078358 1104 28-29 0.5774841790895877 1104 30-31 -0.04153938267798907 1104 32-33 -0.31813146173070805 1104 34-35 0.33596215477796676 1104 36-37 0.4686699428434764 1104 38-39 0.33501002898101717 1104 40-41 1.1671668907221502 1104 42-43 1.1669684597227743 1104 44-45 -2.914466351910505 1104 46-47 0.2189606617454487 1104 48-49 0.8517478347317322 1104 50-51 1.9550289589493204 1104 52-53 -0.35148734260194914 1104 54-55 -0.14390430609454086 1104 56-57 0.8455945810088821 1104 58-59 1.0973920731735092 1104 60-61 0.4809363777600524 1104 62-63 0.7530186111592201 1104 64-65 0.3801126205688945 1104 66-67 0.5533696362815661 1104 68-69 -3.3637695300945722 1104 70-71 -0.5784016004658241 1104 72-73 1.1495661402419728 1104 74-75 -0.954540430824423 1104 76 0.5681875600457786 1105 1 -1.1113734895762875 1105 2 0.3014706288721918 1105 3 -0.11977869014953768 1105 4 0.3014122712140832 1105 5 0.4508426371911973 1105 6 0.7805910089628085 1105 7 1.3153884606009143 1105 8 0.5395639870906876 1105 9 0.4384831001963505 1105 10-11 0.4654123265538246 1105 12-13 -0.23551106224860519 1105 14-15 0.09230637794409091 1105 16-17 0.8796197652518813 1105 18-19 0.8765331367999849 1105 20-21 -0.23681090497272095 1105 22-23 0.07976145936471113 1105 24-25 0.7202058019379365 1105 26-27 0.4020744640044498 1105 28-29 -0.5666741655278713 1105 30-31 0.5000356003958117 1105 32-33 0.5739640020472265 1105 34-35 0.7440740369715968 1105 36-37 0.9134482096004106 1105 38-39 0.6490757026438416 1105 40-41 0.7734422800247884 1105 42-43 0.690707457691623 1105 44-45 0.7545840881707448 1105 46-47 0.4142678723073985 1105 48-49 -0.33731783893109224 1105 50-51 -0.2825937560134477 1105 52-53 -0.1180009681943659 1105 54-55 -0.779684180840647 1105 56-57 0.12080404275566536 1105 58-59 0.3610494022188746 1105 60-61 -0.4425254536549801 1105 62-63 0.5363420708748592 1105 64-65 0.5185054777117486 1105 66-67 0.704182093966061 1105 68-69 1.1924677752541086 1105 70-71 0.7048372282410185 1105 72-73 0.7350751957599115 1105 74-75 -0.7682464226998462 1105 76 -1.023319718220982 1106 1 0.11233682788402888 1106 2 0.373891263792828 1106 3 -0.3566834520542983 1106 4 0.21599560454741606 1106 5 0.21741009750865814 1106 6 0.24368624705804365 1106 7 1.197531317743767 1106 8 1.3441274791541815 1106 9 1.142947385910638 1106 10-11 0.31357700909350683 1106 12-13 -0.0898761416136864 1106 14-15 0.4701833620710758 1106 16-17 0.8475761144582279 1106 18-19 -0.03552043462858734 1106 20-21 -0.31682082560763547 1106 22-23 -0.056002429524177444 1106 24-25 0.5533407225728624 1106 26-27 0.6086220830520759 1106 28-29 -0.3069023401310531 1106 30-31 -0.3486747170645046 1106 32-33 0.797376700459921 1106 34-35 0.7644113385589009 1106 36-37 0.6152339238861231 1106 38-39 -0.0715592179910729 1106 40-41 0.18127958161209534 1106 42-43 0.8040011084852736 1106 44-45 0.9321634532501051 1106 46-47 0.383613110402635 1106 48-49 0.49442819281493655 1106 50-51 0.48774354557385635 1106 52-53 0.13512403180563126 1106 54-55 0.27334161281014246 1106 56-57 0.8203576141842319 1106 58-59 -0.04911924857477601 1106 60-61 0.09889319713867195 1106 62-63 1.134258737541522 1106 64-65 0.30511262056889166 1106 66-67 0.5133090780930516 1106 68-69 1.4013963466826809 1106 70-71 0.23172214887593157 1106 72-73 0.30308114814086196 1106 74-75 -1.5971154703188937 1106 76 -0.3000062261574925 1107 1 -0.006944217867605573 1107 2 0.6206232899366171 1107 3 0.9442969401025643 1107 4 0.6120740359199672 1107 5 1.1442074831295734 1107 6 1.3201568352933393 1107 7 0.689688180488865 1107 8 1.3555653876509126 1107 9 0.3792218957145579 1107 10-11 0.750995309747104 1107 12-13 1.3485225511967656 1107 14-15 0.5827650614174829 1107 16-17 1.6918571602098638 1107 18-19 1.5590874085086668 1107 20-21 1.2658589129544495 1107 22-23 0.0034746946588271044 1107 24-25 0.39370019969704373 1107 26-27 1.1350926712873672 1107 28-29 0.2839473330715663 1107 30-31 0.7632533875106517 1107 32-33 1.388389772355346 1107 34-35 1.0675159137223034 1107 36-37 0.8691554925135776 1107 38-39 1.4743558146886642 1107 40-41 1.4633057254029467 1107 42-43 1.098445552929718 1107 44-45 1.295725544753374 1107 46-47 1.2105738947163545 1107 48-49 0.9280883235338919 1107 50-51 1.5947696893647105 1107 52-53 1.2542416788644601 1107 54-55 0.8079821356859611 1107 56-57 1.2532007514391381 1107 58-59 1.5682010128631347 1107 60-61 0.7541219553086052 1107 62-63 0.34945481597289785 1107 64-65 1.3433479146865395 1107 66-67 1.6842241107727816 1107 68-69 1.8901218368787553 1107 70-71 1.2294345671765896 1107 72-73 1.2471987951996866 1107 74-75 0.7950413924262065 1107 76 0.44770619214316554 1108 1 0.5378809286858619 1108 2 0.4440516303449442 1108 3 0.33695915962955425 1108 4 0.4383323743068672 1108 5 0.3692428581043039 1108 6 0.11104019894808204 1108 7 1.0166034826922186 1108 8 1.2467620725104211 1108 9 0.1053185199312523 1108 10-11 0.3810741454050728 1108 12-13 0.45760381256729943 1108 14-15 1.2048339920825342 1108 16-17 0.10665400678354331 1108 18-19 0.4802012148559527 1108 20-21 1.4155961274278681 1108 22-23 0.6230926449317238 1108 24-25 0.20004748087755786 1108 26-27 -0.1105359925492948 1108 28-29 0.4979659302011328 1108 30-31 0.9732038625460362 1108 32-33 1.354707742842514 1108 34-35 0.5274411209185814 1108 36-37 0.6747700063603546 1108 38-39 1.2394659824671166 1108 40-41 1.0961134876831125 1108 42-43 0.966572700696041 1108 44-45 0.9032402001000506 1108 46-47 0.6058639695091657 1108 48-49 0.599353736915738 1108 50-51 0.7842784825727094 1108 52-53 0.8389900111870787 1108 54-55 0.9140059885260641 1108 56-57 1.171130810060525 1108 58-59 0.06665967467836964 1108 60-61 1.4849183975968643 1108 62-63 0.71321062757589 1108 64-65 1.0167105587132284 1108 66-67 1.0090421823313065 1108 68-69 1.8714994394661844 1108 70-71 2.0206969484177435 1108 72-73 1.1485278835360546 1108 74-75 2.0068364197154693 1108 76 1.8923190888482395 1109 1 -0.8327017936251799 1109 2 -0.7180576369796015 1109 3 0.7223718064127276 1109 4 0.0826622712140832 1109 5 0.3229062353577312 1109 6 0.4877147675571507 1109 7 -0.8214526394754813 1109 8 0.4380074553871012 1109 9 0.9015035356432577 1109 10-11 1.0611557375545857 1109 12-13 0.966838059218162 1109 14-15 0.8138256674780919 1109 16-17 -0.3054690429986948 1109 18-19 0.17316940494360722 1109 20-21 1.0082831553786988 1109 22-23 1.5008008978673857 1109 24-25 1.1989586667202161 1109 26-27 0.8171336695048339 1109 28-29 0.5357298642658606 1109 30-31 0.3757756691505847 1109 32-33 1.4340671342091795 1109 34-35 0.4690310652374592 1109 36-37 1.0257954212123295 1109 38-39 0.8210581319792141 1109 40-41 0.40581909438689934 1109 42-43 0.7479553568512856 1109 44-45 0.4305294663219996 1109 46-47 0.42670580202473474 1109 48-49 0.4191756675624063 1109 50-51 1.3581386733219247 1109 52-53 1.4908726949072388 1109 54-55 0.9412263424595793 1109 56-57 1.3285126943981354 1109 58-59 1.036338267765089 1109 60-61 0.8953875524565547 1109 62-63 0.7848380601796734 1109 64-65 0.6742302676277205 1109 66-67 1.2249995118423058 1109 68-69 1.9781343145971206 1109 70-71 0.46717966521580934 1109 72-73 -0.03912027788409489 1109 74-75 2.0236510180946468 1109 76 -0.4462332017962325 1110 1 -0.007802061004859695 1110 2 0.183613486015048 1110 3 -0.16085011872096544 1110 4 0.3076622712140846 1110 5 0.3347712086197667 1110 6 0.27076958039138077 1110 7 1.0162813177437684 1110 8 1.7288497013764044 1110 9 -1.7508026140893662 1110 10-11 -0.8006591020176046 1110 12-13 -0.005153919391467809 1110 14-15 -0.49787219348447564 1110 16-17 -0.0020766633195492545 1110 18-19 0.5613545653714169 1110 20-21 -0.47966804782985406 1110 22-23 0.1453864593647083 1110 24-25 0.2224379447950824 1110 26-27 -0.4903362502812598 1110 28-29 -0.036763451242165957 1110 30-31 0.2777141718243783 1110 32-33 0.7918211449043682 1110 34-35 -0.4987831058855363 1110 36-37 0.1058589238861245 1110 38-39 -0.09065644021329433 1110 40-41 0.19308513716764963 1110 42-43 0.21615388626305077 1110 44-45 0.47556623102788365 1110 46-47 0.16069644373596503 1110 48-49 0.3805393039260494 1110 50-51 -0.07232589887058793 1110 52-53 -0.09300096819436021 1110 54-55 -0.39575560941207755 1110 56-57 0.03598261418423476 1110 58-59 -3.067522026352556 1110 60-61 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0.3006813252874494 2116 44-45 1.2282339139547105 2116 46-47 0.8309708339798689 2116 48-49 0.8116368649016508 2116 50-51 0.23537227186112375 2116 52-53 0.5759776903422207 2116 54-55 -0.2025391459974486 2116 56-57 0.316089321501309 2116 58-59 0.11883468096451821 2116 60-61 -0.26967048849818553 2116 62-63 0.4486896318504705 2116 64-65 0.8923077425201171 2116 66-67 0.6388510835131029 2116 68-69 0.45749390765828934 2116 70-71 -0.7978848971945283 2116 72-73 0.6309266765961468 2116 74-75 -0.5503675028392152 2116 76 -0.18022302832551418 2117 1 -0.06875985321265432 2117 2 0.813645953547514 2117 3 -0.04997349534434292 2117 4 0.056688245240057 2117 5 0.42527445537301034 2117 6 0.3731234764952731 2117 7 -1.3875336173211679 2117 8 -1.4466373116106084 2117 9 0.47216816513141424 2117 10-11 -0.22188475136825758 2117 12-13 0.3602681585306158 2117 14-15 -0.21329427140655355 2117 16-17 0.38246067434278785 2117 18-19 0.12389514978699623 2117 20-21 -0.45008200886882577 2117 22-23 -0.583996657518405 2117 24-25 0.35900450323664046 2117 26-27 0.4321069315369286 2117 28-29 0.44367486044615134 2117 30-31 -0.5539416723314687 2117 32-33 0.6113016643848823 2117 34-35 -0.2823058331582686 2117 36-37 -0.38444140078919986 2117 38-39 -0.05709312852498272 2117 40-41 -0.4177103173778036 2117 42-43 -0.43997436049019 2117 44-45 0.9404607115473596 2117 46-47 -0.06259901080948538 2117 48-49 -0.054850306463563925 2117 50-51 0.5254419582722747 2117 52-53 0.6711629928446001 2117 54-55 -0.5274520379835081 2117 56-57 -0.37087615204953295 2117 58-59 -0.35059832505385913 2117 60-61 0.6429047411502182 2117 62-63 0.00752713580993003 2117 64-65 0.5034892439455234 2117 66-67 0.4022340420180086 2117 68-69 0.5552924505787828 2117 70-71 0.6711521633059476 2117 72-73 0.4536222736819866 2117 74-75 0.8498542266507982 2117 76 -0.27129049744176115 2118 1 -0.9280672125200127 2118 2 -0.6993410594394973 2118 3 -0.3703198156906602 2118 4 -0.49309530454349115 2118 5 -0.9881454580469011 2118 6 0.083080186451987 2118 7 -0.4524686822562316 2118 8 -3.8881957531690503 2118 9 -1.0949314019681538 2118 10-11 -0.9058674353509346 2118 12-13 -0.013108464846013135 2118 14-15 -0.7414327995450876 2118 16-17 -0.1673228754407674 2118 18-19 -0.3090052831134287 2118 20-21 -1.8299521387389532 2118 22-23 -0.815598389120133 2118 24-25 -1.1918612976291598 2118 26-27 -0.0906203411903519 2118 28-29 -0.8074073906361008 2118 30-31 -0.6870585554483455 2118 32-33 -0.3854515823683613 2118 34-35 0.3767850759326379 2118 36-37 -0.49158425793205396 2118 38-39 -0.7172662886981414 2118 40-41 -1.478316377983866 2118 42-43 -0.5038271743430087 2118 44-45 -0.3355133144266631 2118 46-47 -0.5625990108094854 2118 48-49 -1.247490999104258 2118 50-51 -0.5892766564463443 2118 52-53 -1.464118392436788 2118 54-55 -0.12160788213935092 2118 56-57 0.9408121596387815 2118 58-59 -0.036745511201040415 2118 60-61 -1.2293896311441586 2118 62-63 0.450167828450617 2118 64-65 -0.4759479854917146 2118 66-67 -0.9430040532200863 2118 68-69 0.5120024072887404 2118 70-71 -1.575601083447296 2118 72-73 -1.1643430942833817 2118 74-75 -0.13499425819768618 2118 76 -0.21717794332920448 2119 1 -2.352023759118069 2119 2 -2.3519997215321204 2119 3 -3.295991628154926 2119 4 -2.0305452759557276 2119 5 -2.2365731310028742 2119 6 -1.6550558913067377 2119 7 -3.6788837765958533 2119 8 -2.3581786005103886 2119 9 -2.778750727296913 2119 10-11 -2.6763076869232663 2119 12-13 -3.679918070334864 2119 14-15 -2.7957495519750424 2119 16-17 -3.3136922293572866 2119 18-19 -4.064294019534245 2119 20-21 -3.7248921044336356 2119 22-23 -3.6274908991258528 2119 24-25 -4.076795545770956 2119 26-27 -3.796881061602015 2119 28-29 -2.0566926965251824 2119 30-31 -2.72865375270392 2119 32-33 -2.8327071569824263 2119 34-35 -2.536165181357241 2119 36-37 -4.889954755359156 2119 38-39 -3.651800308137826 2119 40-41 -2.6990139194361227 2119 42-43 -3.287560736378456 2119 44-45 -3.615530467085321 2119 46-47 -2.1646573298489358 2119 48-49 -3.0765361677720655 2119 50-51 -3.3128329743422853 2119 52-53 -3.0257250247981418 2119 54-55 -3.4290692886573595 2119 56-57 -3.4442650273252013 2119 58-59 -2.891519667861992 2119 60-61 -2.585593176026947 2119 62-63 -3.557407929125141 2119 64-65 -2.384038322827333 2119 66-67 -3.6172464774625084 2119 68-69 -4.237282898600341 2119 70-71 -3.470112232675426 2119 72-73 -3.508396839280522 2119 74-75 -3.806234967174241 2119 76 -2.0608028718597957 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 7.0 13 56.0 14 211.0 15 436.0 16 548.0 17 644.0 18 650.0 19 536.0 20 475.0 21 383.0 22 330.0 23 310.0 24 331.0 25 392.0 26 419.0 27 503.0 28 592.0 29 766.0 30 1023.0 31 1290.0 32 1764.0 33 2529.0 34 3787.0 35 6368.0 36 12396.0 37 18820.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 34.08494543875206 23.88389221665249 13.384245100345646 28.646917244249803 2 16.486403800817033 22.51516187665341 36.75922759911458 24.239206723414977 3 16.306440873180126 27.816869724836685 29.441934961397955 26.43475444058524 4 12.008926161210791 19.247035110767182 37.85880108697609 30.885237641045943 5 12.084510590818292 37.333309338276315 36.28952435798226 14.292655712923139 6 25.669911998128388 38.934979394244785 19.342415462414742 16.052693145212086 7 24.86727734086778 31.831842640416074 23.107239908578833 20.193640110137313 8 21.228426944049524 35.81802148757356 20.958482552594166 21.995069015782747 9 22.700523692119422 15.89252613961524 22.9344754980474 38.472474670217935 10-11 23.62283369625857 26.351071679234078 27.721489373189122 22.30460525131823 12-13 22.636636852808323 23.890978458437562 26.564327748483812 26.908056940270303 14-15 20.616552990084042 25.36127557723109 24.660319974085336 29.36185145859953 16-17 21.597350945705184 27.932045998524302 26.201702449295443 24.268900606475068 18-19 21.61084816527795 27.040329692083432 26.327676498641278 25.02114564399734 20-21 20.839707020353806 27.22479169291126 26.693901056382387 25.241600230352546 22-23 21.74312091709108 27.27248186873504 26.603019777925745 24.381377436248133 24-25 20.81811146903738 28.292871668436305 26.45814962117804 24.430867241348285 26-27 20.250328432342936 28.176795580110497 27.46054312811561 24.112332859430957 28-29 21.25722101247143 27.375960552126262 28.372955171234725 22.993863264167583 30-31 21.29321359799881 27.24818687350406 27.61531124588335 23.84328828261378 32-33 20.003779221480375 28.551118469595266 27.395756474166323 24.04934583475804 34-35 21.61444742383069 28.206489463170588 27.06192524339986 23.117137869598864 36-37 21.839401083376824 27.391257400975398 26.791081037306313 23.97826047834146 38-39 20.908092932855833 27.65940216315439 27.97073802796624 23.46176687602354 40-41 20.952183850126875 27.120413194881856 27.960840066946208 23.96656288804506 42-43 21.06286105062357 27.016934511490632 27.946443032735257 23.97376140515054 44-45 20.46538413086904 27.075422462972625 28.05802004787014 24.40117335828819 46-47 20.732629078409847 27.26438353699138 28.074216711357458 23.928770673241313 48-49 20.32771249122681 26.876563427933846 28.758975651015888 24.036748429823458 50-51 20.40779599402523 26.822574549642773 28.00313135494088 24.766498101391115 52-53 20.13785160256987 26.646210880558606 28.458437561862254 24.75749995500927 54-55 19.613259668508288 27.434448503608255 28.141702809221304 24.810589018662156 56-57 19.916497201576476 27.26438353699138 28.053520974679213 24.76559828675293 58-59 20.122554753720735 26.762286968884407 28.244281677974335 24.87087659942052 60-61 19.717638166537693 26.980941925963254 27.843864163982218 25.457555743516835 62-63 19.49898320945885 26.916155272013963 28.303669444094513 25.281192074432667 64-65 20.217935105368294 27.394856659528138 27.263483722353193 25.12372451275037 66-67 20.440189320999874 27.138409487645543 27.195997624489355 25.225403566865225 68-69 19.735634459301384 26.76048733960804 27.2922777907751 26.211600410315476 70-71 21.00707254305613 26.77218492990444 26.899958608526642 25.320783918512785 72-73 20.44918746738172 26.710097719869708 26.13511616606979 26.705598646678787 74-75 20.45278672593446 27.20229632695665 26.265589288606545 26.07932765850235 76 21.17623769503482 26.64711069519679 25.876869364910824 26.29978224485756 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 1.0 11 1.0 12 0.0 13 1.0 14 2.0 15 3.0 16 6.5 17 9.0 18 9.0 19 9.0 20 11.0 21 13.0 22 21.5 23 47.5 24 68.0 25 71.0 26 98.5 27 162.5 28 281.5 29 364.0 30 379.5 31 519.5 32 797.0 33 950.0 34 1080.0 35 1377.0 36 1604.5 37 1665.0 38 1984.5 39 2608.5 40 3124.0 41 3491.5 42 3648.0 43 3732.5 44 4013.5 45 4178.0 46 4146.0 47 4110.5 48 3924.0 49 3497.0 50 3221.0 51 3024.0 52 2548.0 53 2065.5 54 1862.0 55 1709.0 56 1371.0 57 1029.0 58 872.0 59 743.5 60 552.5 61 402.5 62 315.0 63 270.5 64 203.0 65 155.5 66 105.5 67 80.0 68 64.5 69 41.5 70 31.0 71 28.0 72 21.5 73 12.0 74 10.5 75 12.0 76 9.0 77 6.5 78 6.5 79 6.0 80 4.0 81 2.5 82 3.0 83 3.0 84 4.5 85 5.0 86 2.5 87 1.0 88 1.0 89 0.5 90 0.5 91 1.0 92 1.0 93 1.0 94 1.0 95 2.0 96 3.0 97 2.0 98 2.0 99 4.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5542858171216729 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 55567.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.298414526607516 #Duplication Level Percentage of deduplicated Percentage of total 1 71.92761500729152 39.05555455576151 2 11.871934243669626 12.892544135908002 3 5.853108842635557 9.534435906203322 4 3.1154712978920855 6.766606079147695 5 2.0780856423173804 5.641837781417028 6 1.2528171814927749 4.081559198805046 7 1.0208139997348535 3.88000071985171 8 0.6860665517698529 2.9801860816671764 9 0.5302929868752485 2.591466157971458 >10 1.6637942463210924 12.575809383267048 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.001799629276369068 0.0 10 0.0 0.0 0.0 0.001799629276369068 0.0 11 0.0 0.0 0.0 0.003599258552738136 0.0 12 0.0 0.0 0.0 0.003599258552738136 0.0 13 0.0 0.0 0.0 0.003599258552738136 0.0 14 0.0 0.0 0.0 0.005398887829107204 0.0 15 0.0 0.0 0.0 0.007198517105476272 0.0 16 0.0 0.0 0.0 0.012597404934583476 0.0 17 0.0 0.0 0.0 0.023395180592797885 0.0 18 0.0 0.0 0.0 0.028794068421905086 0.0 19 0.0 0.0 0.0 0.03419295625101229 0.0 20 0.0 0.0 0.0 0.037792214803750425 0.0 21 0.0 0.0 0.0 0.037792214803750425 0.0 22 0.0 0.0 0.0 0.04679036118559577 0.0 23 0.0 0.0 0.0 0.048589990461964834 0.0 24 0.0 0.0 0.0 0.050389619738333905 0.0 25 0.0 0.0 0.0 0.05218924901470297 0.0 26 0.0 0.0 0.0 0.05758813684381017 0.0 27 0.0 0.0 0.0 0.06478665394928644 0.0 28 0.0 0.0 0.0 0.07018554177839365 0.0 29 0.0 0.0 0.0 0.08098331743660805 0.0 30 0.0 0.0 0.0 0.09178109309482246 0.0 31 0.0 0.0 0.0 0.12417442006946569 0.0 32 0.0 0.0 0.0 0.14756960066226357 0.0 33 0.0 0.0 0.0 0.16736552270232333 0.0 34 0.0 0.0 0.0 0.1961595911242284 0.0 35 0.0 0.0 0.0 0.23395180592797885 0.0 36 0.0 0.0 0.0 0.34372919178649197 0.0 37 0.0 0.0 0.0 0.5272913779761369 0.0 38 0.0 0.0 0.0 0.7378480033113178 0.0 39 0.0 0.0 0.0 0.9376068529882844 0.0 40 0.0 0.0 0.0 1.2543416056292405 0.0 41 0.0 0.0 0.0 1.6070689437975778 0.0 42 0.0 0.0 0.0 2.096568106969964 0.0 43 0.0 0.0 0.0 2.6076628214587796 0.0 44 0.0 0.0 0.0 3.088163838249321 0.0 45 0.0 0.0 0.0 3.6586463188583154 0.0 46 0.0 0.0 0.0 4.247125092231 0.0 47 0.0 0.0 0.0 4.751021289614339 0.0 48 0.0 0.0 0.0 5.251318228444941 0.0 49 0.0 0.0 0.0 5.7606133136573865 0.0 50 0.0 0.0 0.0 6.250112476829773 0.0 51 0.0 0.0 0.0 6.811596811056923 0.0 52 0.0 0.0 0.0 7.3640829989022265 0.0 53 0.0 0.0 0.0 7.855381791350982 0.0 54 0.0 0.0 0.0 8.32328540320694 0.0 55 0.0 0.0 0.0 8.848777151906708 0.0 56 0.0 0.0 0.0 9.361671495671892 0.0 57 0.0 0.0 0.0 9.8673673223316 0.0 58 0.0 0.0 0.0 10.32987204635845 0.0 59 0.0 0.0 0.0 10.731189374988752 0.0 60 0.0 0.0 0.0 11.152302625659114 0.0 61 0.0 0.0 0.0 11.632803642449655 0.0 62 0.0 0.0 0.0 12.158295391149423 0.0 63 0.0 0.0 0.0 12.62799863228175 0.0 64 0.0 0.0 0.0 13.104900390519553 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAACAAG 15 0.0022148744 69.991 45 GTCAGTA 20 7.867497E-5 69.991 56 TCAATGT 15 0.0022148744 69.991 53 AATTCAG 20 7.867497E-5 69.991 18 CCGACCA 20 7.867497E-5 69.991 38 AATAACA 20 7.867497E-5 69.991 28 CGACCAC 25 2.372721E-4 55.9928 39 TTCGTCC 25 2.372721E-4 55.9928 2 GATAGTC 25 2.372721E-4 55.9928 52 GTTCGTC 20 0.0066775316 52.970028 1 TGGCGCA 20 0.006920953 52.493248 54 TTGTAAA 20 0.006920953 52.493248 59 CAATGTT 20 0.006920953 52.493248 54 CAAAATT 35 2.0632504E-5 49.99357 15 ACACCCG 30 5.834651E-4 46.660664 34 ATATCCA 30 5.834651E-4 46.660664 9 ATTCAGA 30 5.834651E-4 46.660664 19 GAGGTAT 30 5.834651E-4 46.660664 22 TCAGAAT 30 5.834651E-4 46.660664 21 ACCCGAC 30 5.834651E-4 46.660664 36 >>END_MODULE