FastQCFastQC Report
Wed 25 May 2016
SRR1780584_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780584_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55567
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT4140.7450465204167941RNA PCR Primer, Index 15 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT2620.4715028704086959No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC1330.23935069375708604No Hit
CTTTACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC560.10077923947666781No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCAG200.006917434352.540
AGCAGTT200.006917434352.542
TCTAATA350.001245482540.02
ATTACCC400.002399734535.019
AAACATC400.002399734535.067
GCCAGTC400.002399734535.054
TGCAGGT400.002399734535.042
GCCATAC450.004273595331.11110924
AATTACC450.004273595331.11110918
ATATTGA500.00715237327.9999988
AATCTCG906.03815E-627.22222133
CTCGTAT1251.0613803E-725.19999939
CGTCTTC1251.0613803E-725.19999945
TATGCCG1251.0613803E-725.19999940
GTAGTAC700.00121191725.03
ATCTCGT1001.3678691E-524.49999834
CCGTCTT1301.5510523E-724.2307744
GCCGTCT1301.5510523E-724.2307743
TACCCTA750.001811264523.3333345
TCTCGTA1051.995724E-523.33333435
Produced by FastQC (version 0.11.5)