##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780584_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 55567 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.856551550380622 34.0 33.0 34.0 27.0 34.0 2 32.78071517267443 34.0 34.0 34.0 31.0 34.0 3 33.15575791386974 34.0 34.0 34.0 32.0 34.0 4 33.28772472870589 34.0 34.0 34.0 33.0 34.0 5 33.314737164144184 34.0 34.0 34.0 33.0 34.0 6 36.87393596919035 38.0 37.0 38.0 35.0 38.0 7 36.47222272211924 38.0 37.0 38.0 34.0 38.0 8 36.35620062267173 38.0 37.0 38.0 34.0 38.0 9 36.16803138553458 38.0 37.0 38.0 34.0 38.0 10-11 35.74837583457808 38.0 36.5 38.0 31.5 38.0 12-13 35.704158943257696 38.0 36.0 38.0 31.0 38.0 14-15 35.96216279446434 38.0 36.5 38.0 32.5 38.0 16-17 36.12058415966311 38.0 37.0 38.0 33.0 38.0 18-19 36.080623391581334 38.0 37.0 38.0 32.5 38.0 20-21 35.797316752748934 38.0 36.0 38.0 31.5 38.0 22-23 35.784476397862036 38.0 36.0 38.0 31.5 38.0 24-25 34.984433206759405 37.0 35.0 38.0 28.5 38.0 26-27 35.26246693181205 37.0 36.0 38.0 30.5 38.0 28-29 35.02398006010762 37.0 35.5 38.0 29.0 38.0 30-31 34.572642035740635 37.0 34.0 38.0 26.5 38.0 32-33 35.22961469937193 37.0 36.0 38.0 30.5 38.0 34-35 35.25665412924938 37.5 35.5 38.0 29.0 38.0 36-37 35.50178163298361 38.0 36.0 38.0 31.0 38.0 38-39 35.215523602137964 37.5 35.5 38.0 29.0 38.0 40-41 34.43201000593878 37.0 34.0 38.0 25.5 38.0 42-43 34.91077438047762 37.0 35.0 38.0 29.0 38.0 44-45 35.19202944193496 37.0 35.5 38.0 29.0 38.0 46-47 35.4486025878669 38.0 36.0 38.0 31.0 38.0 48-49 35.13995716882322 37.5 35.5 38.0 29.0 38.0 50-51 35.47000917810931 37.5 36.0 38.0 31.0 38.0 52-53 35.32486007882376 37.5 36.0 38.0 31.0 38.0 54-55 35.49155973869382 38.0 36.0 38.0 31.0 38.0 56-57 35.60822970468084 38.0 36.0 38.0 31.0 38.0 58-59 35.74126729893642 38.0 36.0 38.0 31.5 38.0 60-61 35.230838447279865 37.5 35.5 38.0 29.0 38.0 62-63 35.54151744740584 38.0 36.0 38.0 31.0 38.0 64-65 35.59839473068548 38.0 36.0 38.0 31.0 38.0 66-67 34.827451544981734 37.0 35.0 38.0 27.0 38.0 68-69 34.15726060431551 37.0 34.0 38.0 24.5 38.0 70-71 34.13861644501233 37.0 34.0 38.0 24.0 38.0 72-73 33.81278456637933 37.0 34.0 38.0 23.5 38.0 74-75 34.72010365864632 37.0 34.5 38.0 27.0 38.0 76 34.15946515017907 37.0 34.0 38.0 25.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.3294131534745581 1101 2 1.2214525704229615 1101 3 0.40305859071860084 1101 4 0.5976849542281855 1101 5 0.5371858614303875 1101 6 -1.1018676549623265 1101 7 0.0590853375841931 1101 8 1.1035626844215187 1101 9 0.9215082123223084 1101 10-11 0.37710135528059396 1101 12-13 1.168963060945444 1101 14-15 0.982081052393653 1101 16-17 0.2809232846902461 1101 18-19 -0.2820310242279831 1101 20-21 0.07176123772673293 1101 22-23 1.068739099753472 1101 24-25 -2.1912566980142714 1101 26-27 -0.04949864218507116 1101 28-29 0.7334539296384861 1101 30-31 1.3688321693053993 1101 32-33 0.7447665285359619 1101 34-35 0.7246662597621665 1101 36-37 0.9344720397737021 1101 38-39 0.42116305794238684 1101 40-41 1.4410618034428921 1101 42-43 0.6123115680414131 1101 44-45 0.49336358593449603 1101 46-47 1.1116555781112325 1101 48-49 -1.0442314704540792 1101 50-51 -1.9035027185515574 1101 52-53 -2.5427168511167366 1101 54-55 -0.7230855229974438 1101 56-57 -0.8576427335735275 1101 58-59 -1.4674808572674536 1101 60-61 -2.2033313481531493 1101 62-63 -0.47149819378537217 1101 64-65 -0.7922033826777763 1101 66-67 -0.848448429571171 1101 68-69 -0.9445361559360492 1101 70-71 -0.37831762607792285 1101 72-73 -2.500899881140871 1101 74-75 -0.9870383066520603 1101 76 -0.3472213907277748 1102 1 -0.5035572091804958 1102 2 0.6592267710984032 1102 3 0.6327716860694181 1102 4 0.6002730929776732 1102 5 0.17651925788905487 1102 6 0.7344483504664581 1102 7 0.3058107108127359 1102 8 1.0657506085448674 1102 9 1.1787439538871851 1102 10-11 1.3490105810483541 1102 12-13 0.8647936325453571 1102 14-15 0.4671203479149142 1102 16-17 0.0962027721125267 1102 18-19 0.16497525674287772 1102 20-21 1.0577474132782854 1102 22-23 1.2109920429722365 1102 24-25 -3.994794873060833 1102 26-27 -0.24667062716124377 1102 28-29 0.6516908390220664 1102 30-31 2.0322005371998557 1102 32-33 0.26272568644398575 1102 34-35 0.937966767921111 1102 36-37 -2.602077529395885 1102 38-39 0.11713566154683264 1102 40-41 1.2421854344121854 1102 42-43 1.3983324625274136 1102 44-45 1.3619839817303543 1102 46-47 2.043054119915986 1102 48-49 -3.0705073912949032 1102 50-51 -0.05533493141019363 1102 52-53 0.38342650651984655 1102 54-55 1.026322676983618 1102 56-57 1.175024627471828 1102 58-59 0.41368569892988205 1102 60-61 -5.6750827605825265 1102 62-63 -1.7330895834896296 1102 64-65 0.37908721480351915 1102 66-67 1.8531896728812498 1102 68-69 -0.6998939187741584 1102 70-71 -0.826570632422019 1102 72-73 -5.68402647900092 1102 74-75 -1.8676998601665744 1102 76 -0.10898637510425147 1103 1 1.6638867967648672 1103 2 0.9848123382143896 1103 3 0.15504117507562398 1103 4 0.5311582346925974 1103 5 0.3492679637472875 1103 6 0.4869469410051295 1103 7 0.9804557828233982 1103 8 0.678097489791341 1103 9 0.12759332091091835 1103 10-11 0.5877477036971115 1103 12-13 -0.5175035331560451 1103 14-15 -0.25369098927949096 1103 16-17 0.2805475007271525 1103 18-19 0.9153980951417324 1103 20-21 -0.14577880270244492 1103 22-23 0.6123041233371609 1103 24-25 -2.8143453830295684 1103 26-27 -0.3294188204880797 1103 28-29 -0.18755368970532516 1103 30-31 1.6623194444829252 1103 32-33 1.732845106258452 1103 34-35 0.8425846757679665 1103 36-37 -0.29542999723454244 1103 38-39 0.17630741081648438 1103 40-41 0.01318640822182715 1103 42-43 -0.4195523204155407 1103 44-45 1.5812192848926756 1103 46-47 1.5291645191779466 1103 48-49 -2.3087704224055656 1103 50-51 0.11301881775694511 1103 52-53 0.468673290385361 1103 54-55 -0.20839107423364567 1103 56-57 0.6463613093435896 1103 58-59 -0.10307407760643628 1103 60-61 -4.601688265169685 1103 62-63 -0.7440193154357146 1103 64-65 0.20344270657739116 1103 66-67 -0.07477403989614828 1103 68-69 0.9968156296856847 1103 70-71 1.0213869299786822 1103 72-73 -4.154491857505356 1103 74-75 -1.4736785388606393 1103 76 -2.639866391505258 1104 1 0.7809353640938816 1104 2 1.0657506398796812 1104 3 0.5832337051856271 1104 4 0.13264090785230565 1104 5 -0.6879727858505191 1104 6 0.5375143569797345 1104 7 0.7026176159465791 1104 8 1.2217515617075136 1104 9 1.3516900207674354 1104 10-11 -0.4237527963246279 1104 12-13 0.5903489821707097 1104 14-15 1.0506809399348214 1104 16-17 0.6809018639603295 1104 18-19 1.2119979473092144 1104 20-21 0.20377987376174644 1104 22-23 0.5278656694913408 1104 24-25 0.9501335073569663 1104 26-27 1.6756466171396411 1104 28-29 1.9784535279997968 1104 30-31 0.6680262143424827 1104 32-33 0.7857604504038491 1104 34-35 0.3126920717417079 1104 36-37 1.8315320445933665 1104 38-39 1.9370143980767907 1104 40-41 2.753001182777247 1104 42-43 1.2182906292779236 1104 44-45 1.411429294023499 1104 46-47 1.596232652575047 1104 48-49 0.7332205337229638 1104 50-51 0.854670628705243 1104 52-53 0.8897676857938563 1104 54-55 0.20558344639768933 1104 56-57 0.8243799188483507 1104 58-59 -0.25236317713250855 1104 60-61 1.137713447948279 1104 62-63 1.1160724276835552 1104 64-65 1.4932813952160302 1104 66-67 0.6164081854179884 1104 68-69 2.171323216972084 1104 70-71 2.377445889063857 1104 72-73 1.8367577620433124 1104 74-75 0.6653166348425614 1104 76 0.11699260236404285 1105 1 1.7562442672760064 1105 2 0.7727792451606561 1105 3 0.71684491033119 1105 4 0.06593051516442472 1105 5 -0.0728322983759142 1105 6 0.4403791166276676 1105 7 1.0561848129675653 1105 8 -0.517225249396077 1105 9 1.064642796664522 1105 10-11 0.9455663472095637 1105 12-13 -0.14795078086246605 1105 14-15 0.6832765729747621 1105 16-17 0.6770088375554622 1105 18-19 1.1134239798075214 1105 20-21 0.6478810924482801 1105 22-23 0.5347525178325725 1105 24-25 1.081808778176196 1105 26-27 0.5625372738762664 1105 28-29 1.21476446232613 1105 30-31 1.8318189530019353 1105 32-33 0.9860397327328911 1105 34-35 -0.48627754572613924 1105 36-37 1.078464170524306 1105 38-39 0.35356822339839056 1105 40-41 0.9273410609085886 1105 42-43 0.9500209102528743 1105 44-45 1.1723890096633127 1105 46-47 1.5276286410652276 1105 48-49 0.1978236142916856 1105 50-51 1.4271570877438435 1105 52-53 0.8649708002149765 1105 54-55 1.2730555181647887 1105 56-57 0.8428717942715096 1105 58-59 0.4594259223935637 1105 60-61 1.552595810846043 1105 62-63 0.104911713397847 1105 64-65 1.2994658061842088 1105 66-67 1.3410188827774974 1105 68-69 0.9241583557669415 1105 70-71 2.1391990204111764 1105 72-73 2.2610553246702665 1105 74-75 0.6558062252284813 1105 76 1.5730267932915964 1106 1 -0.3436565263747866 1106 2 0.9411324197638322 1106 3 0.8679361801724639 1106 4 0.36821226119617023 1106 5 0.4630804000367874 1106 6 0.8817481642467158 1106 7 -0.5089937584610027 1106 8 0.6304930045721804 1106 9 -0.8170040287323062 1106 10-11 0.865754839273059 1106 12-13 1.818021441359754 1106 14-15 -0.004124220676033019 1106 16-17 0.5928818534284801 1106 18-19 0.9166977893313302 1106 20-21 0.6647461718133627 1106 22-23 1.1281552956103553 1106 24-25 0.46985441309683296 1106 26-27 1.7230531468921413 1106 28-29 1.4017684305801055 1106 30-31 1.9069677625257455 1106 32-33 1.1715556057487646 1106 34-35 1.7650617399881554 1106 36-37 1.9160633768735167 1106 38-39 0.8995999694301418 1106 40-41 2.072182330749861 1106 42-43 1.6028482912052553 1106 44-45 1.8626667874410927 1106 46-47 1.5857139585255524 1106 48-49 2.1068513920694656 1106 50-51 0.8325142306009852 1106 52-53 1.3193358795800663 1106 54-55 0.8461705975298628 1106 56-57 1.20497496887468 1106 58-59 1.130259255726898 1106 60-61 1.6360283505285835 1106 62-63 1.7216279832391095 1106 64-65 -0.19780601921262075 1106 66-67 0.6370506288092415 1106 68-69 1.9926107367193282 1106 70-71 1.0030383061254682 1106 72-73 0.7481584992734369 1106 74-75 0.39097487602213477 1106 76 0.9748125075773117 1107 1 1.4501343232984176 1107 2 -0.2301094103015302 1107 3 0.41336101677377 1107 4 0.5842253330915952 1107 5 0.409485628794954 1107 6 0.4493952230702476 1107 7 1.1949278101664476 1107 8 0.48408777581401097 1107 9 -0.14412821173883827 1107 10-11 0.87033000267175 1107 12-13 0.4433482387453793 1107 14-15 0.32201957017364435 1107 16-17 0.6356923109448189 1107 18-19 1.065717397174474 1107 20-21 0.9621317927283854 1107 22-23 0.3077304590090364 1107 24-25 0.2376648706131732 1107 26-27 -0.029724630885631598 1107 28-29 1.4426181037827206 1107 30-31 1.3138305076237842 1107 32-33 1.415836651500399 1107 34-35 0.8077087988116816 1107 36-37 1.6069130500761233 1107 38-39 1.1567895119138 1107 40-41 1.5509405006844972 1107 42-43 1.2136326049307442 1107 44-45 0.526555676329977 1107 46-47 1.4497662461072522 1107 48-49 1.417799104487763 1107 50-51 1.6300632502088277 1107 52-53 1.1706430691225478 1107 54-55 1.0352228851115655 1107 56-57 1.2842233348877556 1107 58-59 1.0832004321974864 1107 60-61 1.7807995923586475 1107 62-63 1.5082292904286518 1107 64-65 1.5971286213102616 1107 66-67 1.2153186026654552 1107 68-69 2.0195715210330434 1107 70-71 0.9099010512235068 1107 72-73 1.8984852966590609 1107 74-75 -0.2229140128667524 1107 76 1.465008586008686 1108 1 -0.4430837886886465 1108 2 0.6805940463388609 1108 3 -0.006691540331544843 1108 4 0.5458182176681063 1108 5 0.23690628778020084 1108 6 -0.05570888042682043 1108 7 0.0430680972091011 1108 8 -0.5538139828275916 1108 9 -0.13836714442531672 1108 10-11 0.6258636594334277 1108 12-13 1.2705414871787681 1108 14-15 0.86217016649465 1108 16-17 0.011324006922180274 1108 18-19 0.8564744216337417 1108 20-21 1.4257713722715408 1108 22-23 0.15089298175009702 1108 24-25 1.1008395219169955 1108 26-27 -0.6499995449749818 1108 28-29 -0.6565076289846132 1108 30-31 0.6191670752405187 1108 32-33 1.1072658004795812 1108 34-35 0.9001132863799057 1108 36-37 1.7792649805390326 1108 38-39 1.6643193279639306 1108 40-41 1.31430718756544 1108 42-43 0.20602698536333008 1108 44-45 1.3018993189416648 1108 46-47 1.6445054820077942 1108 48-49 1.137578768930858 1108 50-51 1.4379266017350005 1108 52-53 0.18989716251248012 1108 54-55 -0.19228873809441893 1108 56-57 0.27227164699610285 1108 58-59 0.8144516955894403 1108 60-61 1.4202780641597457 1108 62-63 1.3557631493330362 1108 64-65 0.9352123083933392 1108 66-67 1.548677203837883 1108 68-69 1.7834182968567802 1108 70-71 -0.4943843742869021 1108 72-73 0.25359950729176717 1108 74-75 -0.4686184802207052 1108 76 1.6191424044845277 1109 1 -1.8979048923878565 1109 2 0.3862007501262781 1109 3 0.35810254975059763 1109 4 0.0646174899543368 1109 5 0.2334606733582376 1109 6 -0.2916921209582739 1109 7 -0.7493680900118065 1109 8 0.3183123747444938 1109 9 0.8157648363895049 1109 10-11 0.41377920053270856 1109 12-13 0.25061205336213277 1109 14-15 0.7928306218670471 1109 16-17 0.7711646817113049 1109 18-19 0.8573840638411383 1109 20-21 1.1036202062756217 1109 22-23 0.2602705659609157 1109 24-25 -0.47557042350447176 1109 26-27 -0.18569611038652312 1109 28-29 -0.33969027411389163 1109 30-31 0.20955243275746938 1109 32-33 0.6756405730690318 1109 34-35 0.07174623196676322 1109 36-37 1.500183994817668 1109 38-39 1.32702124096906 1109 40-41 0.6443005719857453 1109 42-43 1.3261548865706771 1109 44-45 0.762963875973476 1109 46-47 0.8635362995831812 1109 48-49 0.5439131864841968 1109 50-51 0.08639123594857523 1109 52-53 -0.14486495226783092 1109 54-55 -0.6951692717511833 1109 56-57 0.13025720298044519 1109 58-59 -0.3945179278738138 1109 60-61 0.47197606294688654 1109 62-63 -0.16200243862660813 1109 64-65 0.570390653395819 1109 66-67 -0.08659761836840829 1109 68-69 -0.6934409566853077 1109 70-71 0.5805784130773404 1109 72-73 -0.28912611154058254 1109 74-75 -1.0602580413872502 1109 76 0.1780210637270443 1110 1 -1.1151843041525638 1110 2 -1.0040064691250592 1110 3 -0.2626193753830961 1110 4 0.1355733723072774 1110 5 0.5262748444812289 1110 6 0.5046648309133843 1110 7 0.9285062415389973 1110 8 1.2568818934610704 1110 9 1.4816070823788081 1110 10-11 -0.4588979385047196 1110 12-13 0.648924219137534 1110 14-15 1.3149730015461927 1110 16-17 0.48906240898403297 1110 18-19 -0.41246887733533555 1110 20-21 0.9175239495911356 1110 22-23 -0.46837248216742466 1110 24-25 -1.1547983646809428 1110 26-27 1.3754837024476956 1110 28-29 -0.13434268053100595 1110 30-31 -0.4221989041409273 1110 32-33 1.0021111613043203 1110 34-35 1.2556867399881497 1110 36-37 1.7914105990957339 1110 38-39 1.607586080541246 1110 40-41 -1.3614982248056933 1110 42-43 0.3643066245385924 1110 44-45 1.6348890096633184 1110 46-47 1.2718250696366553 1110 48-49 1.7634486142916828 1110 50-51 0.045014230600976646 1110 52-53 1.1377386573578434 1110 54-55 -0.054176624692360065 1110 56-57 0.7442110799857886 1110 58-59 -0.23744907760643486 1110 60-61 -0.17508276058252648 1110 62-63 -0.5089275723164448 1110 64-65 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2116 58-59 -2.2738057849235105 2116 60-61 -1.101912028875212 2116 62-63 -0.18880562109693244 2116 64-65 -2.0714510056624817 2116 66-67 -1.149399235689394 2116 68-69 -1.2258851982400287 2116 70-71 0.4298675744181466 2116 72-73 0.18244034209186566 2116 74-75 0.01692338550723349 2116 76 -0.04957773632512641 2117 1 0.5642799815617217 2117 2 0.6489967776281844 2117 3 0.15718581942210363 2117 4 -1.2615857186018111 2117 5 0.043239130195516395 2117 6 0.2614018439003942 2117 7 0.1734737740065313 2117 8 -0.007890833811657672 2117 9 -1.2117370734653505 2117 10-11 -0.9292307307125114 2117 12-13 -2.073681300342983 2117 14-15 -0.9737851153369235 2117 16-17 -2.209354798808178 2117 18-19 -1.99899485136131 2117 20-21 -0.48117734911016896 2117 22-23 -1.2126094951544388 2117 24-25 -2.8903908971484746 2117 26-27 -2.521066622227629 2117 28-29 -2.0616154078037354 2117 30-31 -1.386322280764304 2117 32-33 -3.316720007526847 2117 34-35 -0.2953278704014579 2117 36-37 -1.4860650502549149 2117 38-39 -0.20162658179641113 2117 40-41 -0.838892705325172 2117 42-43 -0.26933785598088633 2117 44-45 -1.4436824189081179 2117 46-47 -1.7911619433503532 2117 48-49 -1.392679632461558 2117 50-51 -0.9567714836847259 2117 52-53 -0.3282110179668365 2117 54-55 -1.4140386376793685 2117 56-57 -0.4728749589752397 2117 58-59 -1.9342429087752677 2117 60-61 -1.4543035398033055 2117 62-63 -1.1566548450437182 2117 64-65 -0.7928637392703379 2117 66-67 -1.8505395587809446 2117 68-69 0.0938012129097956 2117 70-71 -0.87358507049791 2117 72-73 0.3884903896053302 2117 74-75 0.45349291354016685 2117 76 0.8709164036812069 2118 1 -0.2820403647586254 2118 2 0.1598192884506986 2118 3 -1.113376951140669 2118 4 0.14750519048909894 2118 5 0.22722181417820053 2118 6 -0.23643365393510152 2118 7 0.5046426051753627 2118 8 0.21938189346106896 2118 9 -3.10134746307574 2118 10-11 -2.000659302141081 2118 12-13 -1.8345038111654972 2118 14-15 -0.4402353317871359 2118 16-17 -3.797017136470515 2118 18-19 -1.6764840288504885 2118 20-21 -1.1521730201058347 2118 22-23 -0.3056830882280366 2118 24-25 0.6269251201675416 2118 26-27 -3.81002766118867 2118 28-29 -1.8494941956825244 2118 30-31 -1.252123146565168 2118 32-33 -1.7084948993017406 2118 34-35 -1.5864534115270033 2118 36-37 -4.101865916055779 2118 38-39 -1.0544404346102638 2118 40-41 0.3688995024670305 2118 42-43 -0.44790928455231693 2118 44-45 -3.5757170509427496 2118 46-47 -1.626659778848186 2118 48-49 -0.6080475978295254 2118 50-51 -2.7803645572778066 2118 52-53 -2.9634924032482246 2118 54-55 -2.946506170146904 2118 56-57 -1.6157321018323856 2118 58-59 -2.2697407442731077 2118 60-61 -1.440234275734042 2118 62-63 -2.800594238983109 2118 64-65 -1.2419979384045376 2118 66-67 -1.0291109873523752 2118 68-69 -2.43541956631098 2118 70-71 -3.1333253302381685 2118 72-73 -2.3983061471912066 2118 74-75 -1.6850352249879634 2118 76 -0.9948844621196571 2119 1 0.2974336203757382 2119 2 -1.71096401629487 2119 3 0.3586070397112451 2119 4 -0.24614832580592605 2119 5 -0.18587138193386465 2119 6 -2.242151206822463 2119 7 -1.5854088528006258 2119 8 -0.05420301219930934 2119 9 0.1705221767184284 2119 10-11 -1.6841927498254776 2119 12-13 -0.7240121016171841 2119 14-15 -0.3191661493972049 2119 16-17 -0.6242041004499299 2119 18-19 -1.374025481108923 2119 20-21 -2.1353062390881092 2119 22-23 -1.3411319161296902 2119 24-25 -0.03716864769981498 2119 26-27 -0.3945045050994693 2119 28-29 -0.40981437864421366 2119 30-31 1.7072822279345488 2119 32-33 0.28607342545526393 2119 34-35 -0.22267410906844987 2119 36-37 -0.553695532979738 2119 38-39 -1.7279681647417675 2119 40-41 -1.1602600172585298 2119 42-43 -0.5152334698010321 2119 44-45 -0.41133740543102704 2119 46-47 -0.7970428548916502 2119 48-49 -2.1553603479724686 2119 50-51 0.40845762682739917 2119 52-53 -0.45477313509499595 2119 54-55 0.28762762059066205 2119 56-57 -2.273536561523642 2119 58-59 -0.8955269077951158 2119 60-61 -0.8260261568089433 2119 62-63 -1.7707200251466375 2119 64-65 -2.169242077912827 2119 66-67 -0.9103288261173788 2119 68-69 -2.247169137494513 2119 70-71 -2.5724333919877402 2119 72-73 -1.494503974521109 2119 74-75 -1.466624704690652 2119 76 -1.2138667377057075 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 3.0 22 13.0 23 21.0 24 30.0 25 95.0 26 142.0 27 322.0 28 543.0 29 845.0 30 1345.0 31 2159.0 32 3333.0 33 4866.0 34 6938.0 35 9787.0 36 13401.0 37 11724.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.463494520128855 25.979448233663867 11.726384364820847 26.830672881386437 2 17.612971727824068 21.043065128583514 38.22592545935537 23.11803768423705 3 17.46900138571454 26.89365990605935 29.764068601868015 25.873270106358092 4 12.90154228228985 19.092266992999445 39.64223369985783 28.36395702485288 5 12.192488347400436 38.31050803534472 36.71963575503446 12.777367862220382 6 27.379559810678998 39.91577734986593 18.38321305811003 14.321449781345045 7 26.116220058667917 31.237964979214283 24.17621969874206 18.469595263375744 8 22.837295517123472 36.32911620206237 21.097054006874583 19.73653427393957 9 23.32499505101949 14.956718915903325 23.504957978656396 38.21332805442079 10-11 24.606331095794268 25.969550272643836 28.122806701819425 21.30131192974247 12-13 24.116831932621878 23.701117569780624 26.63181384634765 25.55023665124984 14-15 21.594651501790633 25.797685676750586 23.8477873558047 28.759875465654076 16-17 22.10034732845034 27.866359529936837 26.241294293375567 23.791998848237263 18-19 21.908686810517032 27.654003275325284 26.940450267244948 23.496859646912736 20-21 21.224827685496788 27.82586787121853 26.463548509007147 24.485755934277538 22-23 22.132740655424982 28.323465366134577 26.927852862310363 22.615941116130077 24-25 22.247916929112602 28.73738009969946 26.564327748483812 22.45037522270412 26-27 21.30671081757158 28.686090665322943 27.68729641693811 22.319902100167365 28-29 21.48307448665575 28.569114762358954 27.99863228174996 21.949178469235335 30-31 21.747619990282 28.12730577501035 27.89695322763511 22.228121007072545 32-33 21.282415822340596 28.898446919934496 27.533428113808554 22.285709143916353 34-35 21.766516097683876 27.820468983389425 28.91914265661274 21.493872262313964 36-37 22.19752730937427 27.511832562492128 27.36516277646805 22.925477351665556 38-39 21.843000341929564 27.755682329440134 27.662101607068944 22.739215721561358 40-41 21.195133802436697 27.294077420051472 28.44673997156586 23.064048805945976 42-43 21.927582917918908 27.12671189734915 27.982435618262635 22.963269566469307 44-45 20.723630932028 27.48213867943204 28.085014487015673 23.709215901524285 46-47 22.03915993305379 26.515737758021846 28.26047834146166 23.184623967462702 48-49 21.164540104738425 26.209800781039107 28.72118343621214 23.904475678010332 50-51 20.517573379883743 26.42305685028884 29.008224305793007 24.05114546403441 52-53 20.56436374106934 26.916155272013963 30.009717998092395 22.509762988824303 54-55 19.86790721111451 27.384058883869923 28.882250256447172 23.865783648568396 56-57 20.589558550938506 26.75328882250256 28.668094372559256 23.989058253999676 58-59 19.916497201576476 28.079615599186567 28.60780679180089 23.396080407436067 60-61 19.962387748123884 28.149801140964957 27.964439325498947 23.923371785412208 62-63 19.088667734446705 28.519624957258806 27.996832652473593 24.3948746558209 64-65 19.759929454532365 29.42213903935789 27.067324131228965 23.750607374880776 66-67 19.53857505353897 29.247574999550093 26.599420519373005 24.614429427537925 68-69 19.53227635107168 29.102704842802385 26.86576565227563 24.499253153850304 70-71 19.961487933485703 28.450339230118594 26.398761855057856 25.189410981337844 72-73 19.816617776737992 28.519624957258806 26.005542858171214 25.658214407831988 74-75 20.235931398131985 28.439541454460382 25.939856389583742 25.38467075782389 76 19.862508323285404 28.911044324869078 26.101823024456962 25.124624327388556 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 0.5 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 1.0 16 1.0 17 0.0 18 1.5 19 6.5 20 9.5 21 9.0 22 19.5 23 32.0 24 50.0 25 66.0 26 125.0 27 204.0 28 279.5 29 335.0 30 418.0 31 567.5 32 782.0 33 930.0 34 1100.5 35 1436.0 36 1786.0 37 1971.0 38 2242.0 39 2946.0 40 3491.5 41 3725.5 42 3847.0 43 4005.0 44 4171.5 45 4265.5 46 4351.0 47 4280.5 48 3971.0 49 3427.5 50 3123.0 51 2877.5 52 2331.5 53 1818.0 54 1605.0 55 1505.5 56 1171.0 57 817.5 58 699.0 59 584.0 60 381.0 61 259.5 62 226.0 63 186.0 64 113.5 65 67.0 66 51.0 67 49.0 68 32.5 69 13.0 70 7.0 71 4.0 72 3.0 73 1.5 74 0.5 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 55567.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.17785736138355 #Duplication Level Percentage of deduplicated Percentage of total 1 70.36359800267248 36.01058182014505 2 12.346156551093607 12.636996778663596 3 6.020113932062733 9.242895963431533 4 3.3265349180673747 6.809797181780554 5 2.106336591884099 5.389889682725359 6 1.6034882903157748 4.923785700145769 7 0.9986637597580702 3.577663001421707 8 0.6329559040720164 2.591466157971458 9 0.5626274702862367 2.591466157971458 >10 2.0254588930304522 14.668778231684273 >50 0.0035164216892889797 0.10077923947666781 >100 0.01054926506786694 1.4559000845825758 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT 414 0.7450465204167941 RNA PCR Primer, Index 15 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT 262 0.4715028704086959 No Hit ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 133 0.23935069375708604 No Hit CTTTACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC 56 0.10077923947666781 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.001799629276369068 0.0 2 0.0 0.0 0.0 0.001799629276369068 0.0 3 0.0 0.0 0.0 0.001799629276369068 0.0 4 0.0 0.0 0.0 0.001799629276369068 0.0 5 0.0 0.0 0.0 0.001799629276369068 0.0 6 0.0 0.0 0.0 0.001799629276369068 0.0 7 0.0 0.0 0.0 0.001799629276369068 0.0 8 0.0 0.0 0.0 0.001799629276369068 0.0 9 0.0 0.0 0.0 0.003599258552738136 0.0 10 0.0 0.0 0.0 0.003599258552738136 0.0 11 0.0 0.0 0.0 0.007198517105476272 0.0 12 0.0 0.0 0.0 0.007198517105476272 0.0 13 0.0 0.0 0.0 0.007198517105476272 0.0 14 0.0 0.0 0.0 0.00899814638184534 0.0 15 0.0 0.0 0.0 0.010797775658214407 0.0 16 0.0 0.0 0.0 0.012597404934583476 0.0 17 0.0 0.0 0.0 0.019795922040059748 0.0 18 0.0 0.0 0.0 0.025194809869166952 0.0 19 0.0 0.0 0.0 0.030593697698274157 0.0 20 0.0 0.0 0.0 0.030593697698274157 0.0 21 0.0 0.0 0.0 0.03419295625101229 0.0 22 0.0 0.0 0.0 0.037792214803750425 0.0 23 0.0 0.0 0.0 0.037792214803750425 0.0 24 0.0 0.0 0.0 0.037792214803750425 0.0 25 0.0 0.0 0.0 0.039591844080119495 0.0 26 0.0 0.0 0.0 0.0449907319092267 0.0 27 0.0 0.0 0.0 0.05398887829107204 0.0 28 0.0 0.0 0.0 0.061187395396548314 0.0 29 0.0 0.0 0.0 0.07738405888386993 0.0 30 0.0 0.0 0.0 0.08638220526571526 0.0 31 0.0 0.0 0.0 0.11697590296398942 0.0 32 0.0 0.0 0.0 0.13857145428041823 0.0 33 0.0 0.0 0.0 0.1529684884913708 0.0 34 0.0 0.0 0.0 0.17816329836053774 0.0 35 0.0 0.0 0.0 0.2069573667824428 0.0 36 0.0 0.0 0.0 0.2969388306008962 0.0 37 0.0 0.0 0.0 0.46970324113232675 0.0 38 0.0 0.0 0.0 0.6550650565983407 0.0 39 0.0 0.0 0.0 0.8350279842352475 0.0 40 0.0 0.0 0.0 1.1049723756906078 0.0 41 0.0 0.0 0.0 1.4217071283315637 0.0 42 0.0 0.0 0.0 1.878812964529307 0.0 43 0.0 0.0 0.0 2.350315834938003 0.0 44 0.0 0.0 0.0 2.7966238954775315 0.0 45 0.0 0.0 0.0 3.332913419835514 0.0 46 0.0 0.0 0.0 3.8764014612989723 0.0 47 0.0 0.0 0.0 4.342505443878561 0.0 48 0.0 0.0 0.0 4.832004607050948 0.0 49 0.0 0.0 0.0 5.32870228732881 0.0 50 0.0 0.0 0.0 5.771411089315601 0.0 51 0.0 0.0 0.0 6.300502096568107 0.0 52 0.0 0.0 0.0 6.836791620926089 0.0 53 0.0 0.0 0.0 7.317292637716631 0.0 54 0.0 0.0 0.0 7.763600698256159 0.0 55 0.0 0.0 0.0 8.267496895639498 0.0 56 0.0 0.0 0.0 8.773192722299207 0.0 57 0.0 0.0 0.0 9.271690031853439 0.0 58 0.0 0.0 0.0 9.701801428905645 0.0 59 0.0 0.0 0.0 10.094120611154102 0.0 60 0.0 0.0 0.0 10.488239422678928 0.0 61 0.0 0.0 0.0 10.918350819731135 0.0 62 0.0 0.0 0.0 11.402451095074415 0.0 63 0.0 0.0 0.0 11.85775730199579 0.0 64 0.0 0.0 0.0 12.289668328324366 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAGCAG 20 0.0069174343 52.5 40 AGCAGTT 20 0.0069174343 52.5 42 TCTAATA 35 0.0012454825 40.0 2 ATTACCC 40 0.0023997345 35.0 19 AAACATC 40 0.0023997345 35.0 67 GCCAGTC 40 0.0023997345 35.0 54 TGCAGGT 40 0.0023997345 35.0 42 GCCATAC 45 0.0042735953 31.111109 24 AATTACC 45 0.0042735953 31.111109 18 ATATTGA 50 0.007152373 27.999998 8 AATCTCG 90 6.03815E-6 27.222221 33 CTCGTAT 125 1.0613803E-7 25.199999 39 CGTCTTC 125 1.0613803E-7 25.199999 45 TATGCCG 125 1.0613803E-7 25.199999 40 GTAGTAC 70 0.001211917 25.0 3 ATCTCGT 100 1.3678691E-5 24.499998 34 CCGTCTT 130 1.5510523E-7 24.23077 44 GCCGTCT 130 1.5510523E-7 24.23077 43 TACCCTA 75 0.0018112645 23.333334 5 TCTCGTA 105 1.995724E-5 23.333334 35 >>END_MODULE