FastQCFastQC Report
Wed 25 May 2016
SRR1780583_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780583_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences577497
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACTAA250.002353937135.193411
TTTGTAC250.002362964435.16596633
TGAAGAC353.1802247E-431.48821816
CGCGGCT353.234089E-431.39818433
GGAACTT300.005706515629.37113814
TACTCGA300.00578444429.2897432
ACAATTG300.00578934329.2846723
TATAGAC454.112219E-529.2846723
TTCGGTA852.6557245E-1028.45785526
CGGTAAG852.6557245E-1028.45785528
CGTTCGG905.0567905E-1026.92120624
TCGTTCG905.0931703E-1026.90018323
ACGTTAC508.2271785E-526.45010416
ACCGAAT508.275943E-526.4271413
AATCGTT508.3053186E-526.41338239
CCAACGT609.709738E-625.67744622
GCGAACT801.3130193E-724.73893228
GAAGACT450.001384776224.48232717
GACGATT450.001405226124.42080932
CGAATTG551.571859E-424.0455515
Produced by FastQC (version 0.11.5)