FastQCFastQC Report
Wed 25 May 2016
SRR1780583_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780583_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences577497
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT80661.3967172123837872No Hit
ATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCTT33960.5880550028831318Illumina PCR Primer Index 11 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC14060.2434644682136877No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC13590.23532589779687169No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG7520.13021712666905627No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTCA207.8559626E-443.99688320
CCGTCTT4600.037.3017144
GCCGTCT4950.034.66420743
TGCATCC353.2138676E-431.43178712
TACCTTG353.217134E-431.42634436
TGTGAAC300.005742118729.3337942
GACGTGT300.005744549429.3312556
CGCCATT2500.029.03794517
TGCCGTC5950.028.83829142
ATTGGCG2550.028.47103721
CTCGCTA407.023449E-427.50281513
CGCAAGG407.023449E-427.50281512
ACGTGTT407.030564E-427.4980537
ATGATTC2650.026.56415440
CGGATGA2650.026.56415437
AGACTTA508.328221E-526.40270211
GTTCATG508.328221E-526.40270211
CGTTAAT756.998016E-826.40041524
CACCGAT508.338041E-526.3981341
AGCGGAT2600.026.2289135
Produced by FastQC (version 0.11.5)