FastQCFastQC Report
Wed 25 May 2016
SRR1780582_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780582_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42844
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTGC150.00221113669.9929945
GGGAAGC150.00221113669.992996
AGTCTTG150.00221113669.9929929
CTTGTCA200.006909359752.49474360
CGAAATC200.006909359752.49474327
ACTGATA200.006909359752.49474326
CTATAGG200.006909359752.49474351
GCTATCG200.006909359752.4947438
CAATTAC200.006909359752.49474334
AAGCGAG200.006909359752.4947439
GCATTTC200.006909359752.49474322
GTTCGAA200.006909359752.49474338
CGAGGTT200.006909359752.49474312
TGTTCGA200.006909359752.49474337
CGAATTC200.006909359752.49474341
TCGAGTG200.006909359752.49474344
GCGCTTG200.006909359752.49474357
TTCGAGT200.006909359752.49474343
TCGAATT200.006909359752.49474340
TATCATT400.002394766834.9964942
Produced by FastQC (version 0.11.5)