##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780582_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 42844 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.95506955466343 34.0 31.0 34.0 12.0 34.0 2 31.411189431425637 34.0 33.0 34.0 23.0 34.0 3 31.48800298758286 34.0 34.0 34.0 23.0 34.0 4 31.917164597143124 34.0 34.0 34.0 23.0 34.0 5 31.85440201661843 34.0 34.0 34.0 23.0 34.0 6 34.53652786854635 38.0 36.0 38.0 25.0 38.0 7 34.219050508822704 38.0 35.0 38.0 24.0 38.0 8 33.45448604238633 37.0 34.0 38.0 21.0 38.0 9 34.01225375781907 37.0 34.0 38.0 22.0 38.0 10-11 34.377264027635135 38.0 35.5 38.0 24.0 38.0 12-13 34.61237279432359 38.0 35.5 38.0 25.0 38.0 14-15 34.31652039958921 38.0 35.0 38.0 23.5 38.0 16-17 34.20501120343572 38.0 35.0 38.0 23.0 38.0 18-19 34.20084492577723 38.0 34.5 38.0 23.0 38.0 20-21 34.2485528895528 38.0 35.0 38.0 23.0 38.0 22-23 34.170257212211745 38.0 34.5 38.0 23.0 38.0 24-25 34.586453178974885 38.0 36.0 38.0 25.0 38.0 26-27 34.581773410512554 38.0 36.0 38.0 24.0 38.0 28-29 34.766443375968635 38.0 36.0 38.0 26.0 38.0 30-31 34.669813742881146 38.0 36.0 38.0 25.5 38.0 32-33 34.26997946036785 38.0 35.0 38.0 23.0 38.0 34-35 34.32954439361404 38.0 35.0 38.0 24.0 38.0 36-37 34.354460367846144 38.0 35.5 38.0 24.0 38.0 38-39 34.768380636728594 38.0 36.0 38.0 26.0 38.0 40-41 34.292269629353 38.0 35.5 38.0 23.5 38.0 42-43 34.55678741480721 38.0 36.0 38.0 24.5 38.0 44-45 33.701346746335545 37.0 34.0 38.0 22.0 38.0 46-47 34.381045187190736 37.5 35.5 38.0 24.0 38.0 48-49 34.2157945103165 37.5 34.5 38.0 23.5 38.0 50-51 34.064069648025395 37.0 34.0 38.0 23.5 38.0 52-53 34.09114461768276 37.0 34.0 38.0 23.5 38.0 54-55 33.83695499953319 37.0 34.0 38.0 22.5 38.0 56-57 33.89308888059005 37.0 34.0 38.0 22.5 38.0 58-59 33.81946130146578 37.0 34.0 38.0 22.5 38.0 60-61 33.85902343385305 37.0 34.0 38.0 22.5 38.0 62-63 33.88997292503034 37.0 34.0 38.0 23.0 38.0 64-65 33.98865652133321 37.0 34.0 38.0 23.5 38.0 66-67 33.87962141723462 37.0 34.0 38.0 23.0 38.0 68-69 33.399589207356925 37.0 33.5 38.0 21.0 38.0 70-71 33.408015124638226 37.0 34.0 38.0 21.0 38.0 72-73 33.569776398095414 37.0 34.0 38.0 22.0 38.0 74-75 33.100259079451035 37.0 32.5 38.0 21.0 38.0 76 32.431472318177576 37.0 31.0 38.0 19.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -3.817847073124714 1101 2 -1.2655693538064448 1101 3 -0.7105881763782165 1101 4 -3.1827272369764223 1101 5 -0.7949782451097391 1101 6 -2.137996403749959 1101 7 -2.528574138101483 1101 8 -1.8187616196575291 1101 9 -2.1960257344640297 1101 10-11 -1.7486657337486307 1101 12-13 -1.4740023457246636 1101 14-15 -0.8595295715430566 1101 16-17 -2.587498852445897 1101 18-19 -1.1225814498007693 1101 20-21 -1.7375684564997087 1101 22-23 -1.5830540440551744 1101 24-25 -1.223720501300697 1101 26-27 -2.190111553656081 1101 28-29 -0.6485466001552425 1101 30-31 -2.0476305981491265 1101 32-33 -0.6848803601228184 1101 34-35 -2.475921937932597 1101 36-37 -0.9356819766749922 1101 38-39 -1.1233110050587527 1101 40-41 0.15230349425817735 1101 42-43 -0.8370815044090278 1101 44-45 -0.5047756220827608 1101 46-47 -0.2629802937515535 1101 48-49 -1.3534420436995305 1101 50-51 -1.7432604140580352 1101 52-53 -0.23315777522032022 1101 54-55 -1.6748853557362153 1101 56-57 -1.4340586387126137 1101 58-59 -0.8066688793891643 1101 60-61 -0.8309440885225499 1101 62-63 -2.276252907959666 1101 64-65 -0.7462593303748193 1101 66-67 -1.6905417929887747 1101 68-69 -0.7866027824935458 1101 70-71 -0.7229539753844776 1101 72-73 -1.106748860716678 1101 74-75 -2.0946928100127025 1101 76 -2.1673116677598934 1102 1 0.23648626020862196 1102 2 -0.1805693538064439 1102 3 -0.9335881763782155 1102 4 0.24227276302357836 1102 5 0.4320217548902612 1102 6 0.12933692958337417 1102 7 1.6967591952318486 1102 8 1.152905047009142 1102 9 0.8303075988693038 1102 10-11 -0.3963324004152966 1102 12-13 -4.795335679057992 1102 14-15 -1.6950295715430599 1102 16-17 -2.7914988524458977 1102 18-19 -1.2972481164674363 1102 20-21 -0.06106845649970438 1102 22-23 -0.9830540440551729 1102 24-25 0.5236128320326401 1102 26-27 -5.668611553656078 1102 28-29 -0.19154660015524172 1102 30-31 0.7312027351842048 1102 32-33 -7.408213693456155 1102 34-35 -0.7040886045992636 1102 36-37 0.8764846899916776 1102 38-39 0.5670223282745823 1102 40-41 0.32630349425817684 1102 42-43 -0.024081504409025456 1102 44-45 -5.935275622082763 1102 46-47 -0.016480293751548913 1102 48-49 0.39855795630047197 1102 50-51 -5.706427080724701 1102 52-53 -1.549657775220325 1102 54-55 1.4042813109304504 1102 56-57 -1.0377253053792828 1102 58-59 1.0563311206108281 1102 60-61 0.8392225781441098 1102 62-63 -4.864586241293001 1102 64-65 -0.723092663708151 1102 66-67 0.29379154034456434 1102 68-69 -7.967436115826878 1102 70-71 0.4762126912821856 1102 72-73 1.2164178059499875 1102 74-75 -6.072692810012704 1102 76 -1.3519783344265583 1103 1 -0.9721872091791326 1103 2 0.23592724483301097 1103 3 0.04005127940409636 1103 4 -0.07796533221451796 1103 5 0.1434163127133914 1103 6 -1.1757651112329484 1103 7 0.5205687190413713 1103 8 1.0190615095941737 1103 9 0.9379606600937933 1103 10-11 0.7096199805370844 1103 12-13 -0.5987370396022129 1103 14-15 -0.043583993311763436 1103 16-17 0.36360318837042627 1103 18-19 0.568738278090386 1103 20-21 -0.48581335445889096 1103 22-23 0.07749017363190092 1103 24-25 0.9797352810122248 1103 26-27 -0.665295227125462 1103 28-29 0.5882153046066634 1103 30-31 0.5324782453882833 1103 32-33 -4.9024313805309845 1103 34-35 -0.023731461742116267 1103 36-37 -0.017477895042340208 1103 38-39 0.35358695412492125 1103 40-41 0.8293647187479749 1103 42-43 1.18765318946852 1103 44-45 -5.095904873783439 1103 46-47 -0.02319798082638158 1103 48-49 0.7665851671848216 1103 50-51 0.6652565927446901 1103 52-53 0.7016177349837562 1103 54-55 0.548924168073313 1103 56-57 0.9859141504030333 1103 58-59 0.758201868910156 1103 60-61 0.8367565917495554 1103 62-63 0.3655157995233296 1103 64-65 0.6644073362918519 1103 66-67 0.19974392129694252 1103 68-69 -2.036398700860893 1103 70-71 0.8848011266563347 1103 72-73 1.117523248126858 1103 74-75 -1.522012537903862 1103 76 -0.8142232323857428 1104 1 -1.6010137397913802 1104 2 0.3347084239713354 1104 3 0.288634045844006 1104 4 0.19782831857913408 1104 5 -0.0304782451097374 1104 6 -1.1428852926388444 1104 7 0.47592586189851716 1104 8 1.9265161581202506 1104 9 -0.4377479566862519 1104 10-11 -0.4873046226375166 1104 12-13 -1.4251967901691032 1104 14-15 -0.5325295715430585 1104 16-17 -0.3810821857792277 1104 18-19 -0.09169256091188771 1104 20-21 0.2896259879447385 1104 22-23 0.09889040038926566 1104 24-25 -0.22222050130069704 1104 26-27 0.07513844634392086 1104 28-29 -1.021407711266356 1104 30-31 -1.6493528203713481 1104 32-33 -1.5005748045672647 1104 34-35 -0.5381163823770407 1104 36-37 -0.36101531000831955 1104 38-39 -0.9711721161698676 1104 40-41 -0.13619650574182174 1104 42-43 0.14258516225764595 1104 44-45 -4.251942288749429 1104 46-47 -0.5345358493071117 1104 48-49 0.1325857340782477 1104 50-51 0.32412847483085017 1104 52-53 -1.1642411085536537 1104 54-55 -1.0373853557362125 1104 56-57 0.057413583509607236 1104 58-59 0.06744223172194808 1104 60-61 -0.6198051996336673 1104 62-63 0.1833304253736685 1104 64-65 -1.036981552597041 1104 66-67 -0.45898623743321565 1104 68-69 -4.445908338049101 1104 70-71 -1.2147595309400359 1104 72-73 -0.04191552738335247 1104 74-75 -1.3650539211238168 1104 76 -0.590867223315449 1105 1 -1.9191443704220106 1105 2 -1.0866504348875239 1105 3 -1.6396692574592961 1105 4 -1.0097542640034511 1105 5 -0.89703229916379 1105 6 0.05681440706085539 1105 7 0.2918042402768961 1105 8 -0.15362648452239114 1105 9 -0.10235005878835324 1105 10-11 -0.4168278959107923 1105 12-13 -2.292407751130064 1105 14-15 -0.7014484904619778 1105 16-17 -0.7428502037972535 1105 18-19 -0.6697706389899665 1105 20-21 -1.5424873754186237 1105 22-23 -0.7019729629740965 1105 24-25 -0.5448556364358268 1105 26-27 -0.8209764185209494 1105 28-29 -1.1138438974525329 1105 30-31 -0.5504414089599337 1105 32-33 -0.24785333309579727 1105 34-35 -0.29721923522989613 1105 36-37 -0.2236279226209419 1105 38-39 -0.4048245185722692 1105 40-41 -0.45545326249857965 1105 42-43 -0.04773015305767103 1105 44-45 0.3353324860253508 1105 46-47 -0.09384515861642484 1105 48-49 -0.298063665321159 1105 50-51 0.014631477833852102 1105 52-53 -1.2608064238689707 1105 54-55 -2.0492096800605353 1105 56-57 -1.3143018819558563 1105 58-59 -0.8105607712810539 1105 60-61 -0.9081873317657951 1105 62-63 -0.3501718268785865 1105 64-65 -0.7657728438883353 1105 66-67 -1.1327850362320149 1105 68-69 0.28109992020915797 1105 70-71 -0.08065667808718757 1105 72-73 -0.7553164282842459 1105 74-75 -1.763233350553243 1105 76 -2.008284640732864 1106 1 -1.251618065491126 1106 2 -0.7422105751804899 1106 3 -0.7799622221797406 1106 4 -0.7155516644573403 1106 5 -0.19412328327767625 1106 6 -1.135675793062937 1106 7 -0.19916955794881375 1106 8 0.7971162429378893 1106 9 0.30785212813138685 1106 10-11 0.5564029685414411 1106 12-13 -0.1894832617551927 1106 14-15 -0.17470514406213766 1106 16-17 -0.025086638705438702 1106 18-19 -1.5511921368236798 1106 20-21 -0.6750951740569562 1106 22-23 0.0413734368608516 1106 24-25 -0.22460599748390564 1106 26-27 -0.47710392006829494 1106 28-29 -1.2440275161857741 1106 30-31 -1.014439758454472 1106 32-33 -0.215720054779311 1106 34-35 -1.106792166940231 1106 36-37 -0.37501022094979675 1106 38-39 -0.2615400126923362 1106 40-41 -0.7091926889479225 1106 42-43 0.15244521314822634 1106 44-45 0.6623388817340299 1106 46-47 0.139340316935467 1106 48-49 0.629855666224131 1106 50-51 -0.07000850566108596 1106 52-53 -0.7982264775103971 1106 54-55 -0.8909158900873564 1106 56-57 0.5692085368599038 1106 58-59 -0.5484398717555834 1106 60-61 -0.06761584424774725 1106 62-63 -0.3921307705550916 1106 64-65 0.24964906657174168 1106 66-67 -0.19393110596587348 1106 68-69 0.300725461781262 1106 70-71 -0.8999005402699751 1106 72-73 -0.37190916606019186 1106 74-75 -1.3066622756615587 1106 76 -0.3979070876072228 1107 1 0.20682425554661776 1107 2 -0.35871620695329653 1107 3 0.30155168376164454 1107 4 0.04524479099560352 1107 5 0.024154622023129235 1107 6 0.48376583401228146 1107 7 -0.3155951171224629 1107 8 1.0276719467760387 1107 9 -0.8951586015968971 1107 10-11 -0.10956083864373767 1107 12-13 0.21701863329631266 1107 14-15 -0.16627083028431855 1107 16-17 0.825284364337314 1107 18-19 1.3288591096397866 1107 20-21 0.7872707043394556 1107 22-23 0.11904385804272266 1107 24-25 -0.10377644535664388 1107 26-27 0.16451781697329437 1107 28-29 -0.45640674001538173 1107 30-31 0.1974323389138064 1107 32-33 1.0380567028142451 1107 34-35 0.8742039361932754 1107 36-37 0.10518515619214241 1107 38-39 0.619178505430753 1107 40-41 0.9944853124399984 1107 42-43 0.6656038102762949 1107 44-45 0.7901614408543054 1107 46-47 0.9336071188358588 1107 48-49 0.47827823602074915 1107 50-51 0.7643479775503579 1107 52-53 0.3747303366677883 1107 54-55 0.22791184706098733 1107 56-57 1.0644308717768922 1107 58-59 0.4834290227087408 1107 60-61 -0.07927975285821987 1107 62-63 0.01717366546690613 1107 64-65 0.21102738291189382 1107 66-67 1.0379057594587806 1107 68-69 1.5198307839400158 1107 70-71 0.04949357706307467 1107 72-73 0.6715168735490522 1107 74-75 0.16192257460268422 1107 76 -0.8360109684591919 1108 1 -0.9105794674909085 1108 2 -0.3295130157782751 1108 3 -0.5092924017303275 1108 4 -0.03202301162431098 1108 5 -0.04720359722241341 1108 6 -0.20470062910207076 1108 7 0.9310314957013333 1108 8 1.186590493018528 1108 9 -0.38718066404149454 1108 10-11 0.2875877873781292 1108 12-13 0.6922230063880122 1108 14-15 0.029090146766797886 1108 16-17 -0.8210763172346347 1108 18-19 -0.5721307455754214 1108 20-21 -0.4827233860771756 1108 22-23 -0.3006596778579933 1108 24-25 -0.9836641632725289 1108 26-27 -0.5476608494307271 1108 28-29 0.197537906887014 1108 30-31 0.4399468666396089 1108 32-33 -0.10938740237634192 1108 34-35 0.0951484846026176 1108 36-37 -0.31289324428062315 1108 38-39 0.5629143470539191 1108 40-41 -0.13003453391083752 1108 42-43 -0.032884321310433506 1108 44-45 -0.12840942489966523 1108 46-47 0.8942591428681581 1108 48-49 0.0788396464413168 1108 50-51 0.29879592397013255 1108 52-53 0.49875771773741917 1108 54-55 0.21173436257364386 1108 56-57 0.5504202345268254 1108 58-59 -0.633457611783534 1108 60-61 0.7505488692239268 1108 62-63 0.795859768096669 1108 64-65 0.5892899653998285 1108 66-67 -0.011020666228212406 1108 68-69 0.9393127104642005 1108 70-71 1.5991587006718575 1108 72-73 0.7823215618185344 1108 74-75 1.06603958435349 1108 76 0.19708269843729198 1109 1 -0.3275208319899612 1109 2 -0.1438672261468703 1109 3 0.7605607597919999 1109 4 0.4002514864278339 1109 5 0.7527664357413251 1109 6 0.6431667168174187 1109 7 -0.187637967888719 1109 8 1.1988270328247381 1109 9 1.2272934144721432 1109 10-11 1.4492349754712208 1109 12-13 0.5500543918639877 1109 14-15 0.19530730788956419 1109 16-17 0.005664268121478244 1109 18-19 0.6488511743127034 1109 20-21 1.261183316549939 1109 22-23 1.6176551758029802 1109 24-25 1.0849603497631364 1109 26-27 0.9499168151382449 1109 28-29 0.633808009773837 1109 30-31 0.8702984798650562 1109 32-33 1.5637721221466805 1109 34-35 1.0213546578120827 1109 36-37 1.0006868176512498 1109 38-39 1.2718095623171308 1109 40-41 0.9954524304283936 1109 42-43 1.6416986374349491 1109 44-45 1.0782882077044746 1109 46-47 0.6841402736243296 1109 48-49 1.0708983818323858 1109 50-51 1.58550554338877 1109 52-53 1.5252535722974017 1109 54-55 0.9471004598666255 1109 56-57 1.6674165385923487 1109 58-59 0.9572176454335235 1109 60-61 1.0673254150235465 1109 62-63 0.8976478012601916 1109 64-65 0.554478258277662 1109 66-67 1.4180823204864055 1109 68-69 2.098787288428447 1109 70-71 1.025414818941762 1109 72-73 0.18547808963792534 1109 74-75 2.3198603814766585 1109 76 0.6490854953606728 1110 1 -0.051534573124712324 1110 2 0.3694306461935568 1110 3 -0.38671317637821545 1110 4 0.39227276302357694 1110 5 0.1570217548902626 1110 6 0.1756910962500413 1110 7 1.5853008618985172 1110 8 2.375300880342472 1110 9 -0.9811507344640304 1110 10-11 -1.0906032337486309 1110 12-13 0.0973726542753397 1110 14-15 -0.052060821543058466 1110 16-17 0.042094897554100896 1110 18-19 0.6717623001992266 1110 20-21 -0.03372470649970438 1110 22-23 0.5646022059448228 1110 24-25 0.570748248699303 1110 26-27 -0.7100178036560791 1110 28-29 0.6092346498447583 1110 30-31 0.24083815185087332 1110 32-33 1.7837133898771782 1110 34-35 0.7188280620674021 1110 36-37 0.28091177332500905 1110 38-39 0.6204077449412466 1110 40-41 0.21927224425817826 1110 42-43 0.29623099559097454 1110 44-45 1.1490993779172385 1110 46-47 0.1303947062484454 1110 48-49 1.0305892063004691 1110 50-51 0.7321145859419644 1110 52-53 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2.128387168614907 2116 46-47 0.6489266829926308 2116 48-49 0.7043719097888399 2116 50-51 0.6169256324535937 2116 52-53 2.1640050154773505 2116 54-55 0.9844169698451779 2116 56-57 0.9492901984966906 2116 58-59 -0.5989014375287027 2116 60-61 -1.2812231582899969 2116 62-63 1.2402587199473132 2116 64-65 1.6896011347414586 2116 66-67 1.159876811662393 2116 68-69 1.5464204733204099 2116 70-71 -1.8227214172449422 2116 72-73 1.8151581160275043 2116 74-75 -0.8273439728033978 2116 76 0.2553860066587106 2117 1 -0.9167188679965079 2117 2 0.9431485949115057 2117 3 0.7619246441346021 2117 4 0.41470866045947474 2117 5 -0.7916961938276863 2117 6 -0.6898938396473966 2117 7 -1.2083690098963515 2117 8 -1.9618385427344514 2117 9 0.48479477835648055 2117 10-11 0.1684111893282889 2117 12-13 0.6290232953009749 2117 14-15 -0.8049654689789563 2117 16-17 0.172603711656663 2117 18-19 -0.35237632159564214 2117 20-21 0.11361103067978462 2117 22-23 -0.1163873773885058 2117 24-25 0.03899744741725186 2117 26-27 0.7297858822413517 2117 28-29 -0.3101363437449862 2117 30-31 -0.380656239174769 2117 32-33 0.9943504091079518 2117 34-35 -0.26274245075310887 2117 36-37 -0.5212717202647354 2117 38-39 0.17920181545406422 2117 40-41 -0.6313888134341283 2117 42-43 -0.5721584274859453 2117 44-45 0.4387628394556984 2117 46-47 0.1812761165048542 2117 48-49 -0.17323691549440667 2117 50-51 0.968893432095804 2117 52-53 1.0192524811899304 2117 54-55 -0.5646289454798037 2117 56-57 -0.2281099207638988 2117 58-59 0.1518439411236514 2117 60-61 1.1491584755800162 2117 62-63 0.9181060663993108 2117 64-65 0.3054329773174871 2117 66-67 -1.5401828186298019 2117 68-69 -0.21967970557046357 2117 70-71 0.3367896143591125 2117 72-73 0.9949434469756255 2117 74-75 0.3670507797308886 2117 76 0.02879089634267018 2118 1 0.6730247217470833 2118 2 0.571353723116637 2118 3 0.45423233644229555 2118 4 0.37624712199793464 2118 5 0.1057397036082115 2118 6 1.0152343654808078 2118 7 0.7147079131805683 2118 8 -4.769530850426758 2118 9 -1.2459744524127458 2118 10-11 -0.3379990670819666 2118 12-13 0.3085104747881573 2118 14-15 -1.1062475202610074 2118 16-17 0.3841421731951229 2118 18-19 -0.31391478313410204 2118 20-21 -3.283824866756113 2118 22-23 0.531048520047392 2118 24-25 0.5069461653659744 2118 26-27 0.8836320360875121 2118 28-29 -0.09218762579626372 2118 30-31 -0.4768100853286157 2118 32-33 1.0648632296207694 2118 34-35 0.7564883184776576 2118 36-37 0.13898468999167335 2118 38-39 -0.2566956204433666 2118 40-41 -0.7980554801007997 2118 42-43 -0.8349789403064563 2118 44-45 0.6567115574044209 2118 46-47 0.2710197062484454 2118 48-49 -0.6796471719046551 2118 50-51 -0.26187579867342237 2118 52-53 -1.487157775220325 2118 54-55 0.3456274647766051 2118 56-57 0.8488131561591814 2118 58-59 1.1005618898416074 2118 60-61 -0.664944088522553 2118 62-63 0.270670168963413 2118 64-65 -0.4637977919132865 2118 66-67 -1.892746921193904 2118 68-69 0.9469869610962007 2118 70-71 -0.3491078215383254 2118 72-73 -1.774287322255141 2118 74-75 -0.2932056305255202 2118 76 -1.035311667759892 2119 1 1.8319990807214417 2119 2 1.3098152615781729 2119 3 0.9952579774679364 2119 4 1.4326573784081944 2119 5 1.2800986779671852 2119 6 2.092157442403888 2119 7 0.7557335542062091 2119 8 2.384315303419392 2119 9 2.3412050347667375 2119 10-11 1.6107188816359823 2119 12-13 1.7149207311984185 2119 14-15 1.9001627361492481 2119 16-17 1.3777319167848745 2119 18-19 1.5155723963530718 2119 20-21 1.041816158884913 2119 22-23 1.9477151867140563 2119 24-25 1.7018179602377614 2119 26-27 2.0092730617285355 2119 28-29 1.752684169075529 2119 30-31 1.3988309403124148 2119 32-33 1.8289657937233272 2119 34-35 2.0616165236058634 2119 36-37 1.9030872540942383 2119 38-39 0.7394582257104787 2119 40-41 1.9916881096427943 2119 42-43 0.7201492648217425 2119 44-45 1.0964551471480064 2119 46-47 1.3210197062484426 2119 48-49 1.567788725531237 2119 50-51 2.218893432095811 2119 52-53 1.5320729940104485 2119 54-55 0.9174223365714838 2119 56-57 1.7693259766720075 2119 58-59 2.419792659072371 2119 60-61 2.531209757631295 2119 62-63 0.9206701689634116 2119 64-65 2.097740669625182 2119 66-67 2.423919745472766 2119 68-69 2.114935679044919 2119 70-71 0.25858448615398544 2119 72-73 0.26802037005255386 2119 74-75 1.6542302669103748 2119 76 2.5877652553170307 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 10.0 13 47.0 14 159.0 15 282.0 16 457.0 17 452.0 18 452.0 19 447.0 20 361.0 21 272.0 22 298.0 23 275.0 24 267.0 25 346.0 26 399.0 27 476.0 28 572.0 29 676.0 30 910.0 31 1179.0 32 1577.0 33 2194.0 34 3355.0 35 5150.0 36 9531.0 37 12699.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 34.78148496240602 22.457706766917294 11.094924812030076 31.665883458646615 2 17.031556343945475 19.9234431892447 38.48146765007936 24.56353281673046 3 16.26132013817571 25.19139202688824 31.911119409952388 26.636168424983666 4 12.547848006722063 20.69134534590608 38.10335169451965 28.657454952852206 5 13.360097096442908 36.94332928764821 35.388852581458316 14.307721034450566 6 26.017645411259455 37.78125291756139 17.911492857809726 18.289608813369433 7 24.677901223041733 32.268228923536554 23.328820838390442 19.725049015031278 8 21.652973578564094 36.86863971617963 19.517318644384275 21.961068060871998 9 23.363831575016338 16.37102044627019 22.62393800765568 37.64120997105779 10-11 23.816637102044627 26.64433759686304 27.25585846326207 22.28316683783027 12-13 23.12459154140603 24.304453365698812 26.29773130426664 26.273223788628513 14-15 21.202502100644196 25.501820558304544 24.19475305760433 29.100924283446922 16-17 21.721828027261694 28.334189151339746 25.96046120810382 23.983521613294744 18-19 21.45341237979647 27.2780319297918 26.661842965175985 24.606712725235738 20-21 20.615488749883298 27.778685463542153 26.70969097189805 24.8961348146765 22-23 22.307674353468396 27.290869199887968 26.821725329100925 23.579731117542714 24-25 21.60862664550462 28.452058631313605 26.326906918121555 23.61240780506022 26-27 20.18018859116796 28.32485295490617 27.409905704416023 24.08505274950985 28-29 21.98557557651013 27.602464755858463 28.401876575483147 22.010083092148257 30-31 21.390393053869854 27.6246382223882 27.52310708617309 23.461861637568855 32-33 20.239706843431986 28.761320138175705 27.366725795910746 23.63224722248156 34-35 22.018252264027634 27.31771076463449 27.73900662869947 22.925030342638408 36-37 22.229483708337224 27.46242180935487 26.863738213052002 23.444356269255906 38-39 21.43240593782093 27.60479880496686 27.936233778358698 23.026561478853516 40-41 21.37172066100271 26.85790309028102 28.44972458220521 23.320651666511065 42-43 21.126645504621415 26.723695266548408 28.410045747362524 23.73961348146765 44-45 20.354075249743257 26.898748949677902 28.23149099057044 24.515684810008402 46-47 20.841891513397442 26.38875921949398 28.951545140509754 23.817804126598823 48-49 20.23153767155261 26.94309588273737 29.025067687424144 23.800298758285873 50-51 20.097329847819996 26.91625431799085 28.30501353748483 24.68140229670432 52-53 19.704042573055737 26.77154327327047 28.81266921856036 24.711744935113437 54-55 19.097189804873494 27.202175333769024 28.595602651479783 25.1050322098777 56-57 19.65502754177948 27.121650639529456 28.50807580991504 24.715246008776024 58-59 19.889599477173 26.845065820184853 28.243161236112407 25.022173466529736 60-61 20.176687517505368 26.75170385584913 27.798524880963495 25.27308374568201 62-63 19.196386891980207 26.675847259826348 28.189478106619365 25.938287741574083 64-65 20.03431052189338 26.72953038931939 27.67832135188124 25.557837736905988 66-67 20.51395761366819 26.794883764354406 27.105312295770702 25.585846326206703 68-69 19.890766501727196 26.654840817850804 26.990943889459434 26.463448790962563 70-71 20.67383997759313 26.80305293623378 26.617496032116517 25.90561105405658 72-73 21.245682009149473 26.550975632527308 25.595182522640275 26.608159835682944 74-75 20.66216973205116 27.081971804686773 25.755064886565215 26.50079357669685 76 21.242180935486882 26.386425170385586 25.53916534403884 26.832228550088693 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 0.5 16 3.0 17 6.0 18 5.0 19 4.0 20 5.5 21 7.0 22 11.5 23 26.0 24 38.5 25 41.0 26 70.0 27 128.0 28 178.0 29 199.0 30 251.5 31 371.5 32 542.0 33 645.0 34 750.0 35 1004.5 36 1254.0 37 1354.0 38 1556.5 39 1965.5 40 2370.0 41 2732.5 42 2897.0 43 2889.5 44 3067.0 45 3204.5 46 3157.0 47 3247.0 48 3196.5 49 2865.0 50 2674.0 51 2448.5 52 1986.0 53 1647.0 54 1545.0 55 1399.5 56 1082.5 57 792.0 58 673.0 59 556.0 60 369.5 61 258.0 62 216.0 63 181.0 64 119.0 65 73.0 66 51.0 67 48.0 68 38.5 69 23.0 70 17.5 71 18.0 72 13.5 73 6.0 74 4.0 75 5.0 76 6.5 77 5.5 78 2.5 79 2.0 80 1.5 81 2.0 82 3.0 83 3.0 84 2.0 85 1.5 86 3.0 87 4.0 88 2.5 89 0.5 90 1.5 91 2.0 92 1.0 93 1.5 94 2.0 95 1.0 96 0.0 97 0.0 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6628699467836803 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 42844.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.94874428157968 #Duplication Level Percentage of deduplicated Percentage of total 1 73.99033892936332 43.61637568854449 2 11.506176750079188 13.565493417981514 3 5.4838454228698135 9.697974045373915 4 3.2982261640798227 7.7770516291662775 5 1.80947101678809 5.3333022126785545 6 1.1601203674374405 4.103258332555317 7 0.7839721254355401 3.234992064233031 8 0.562242635413367 2.651479787134721 9 0.328634779854292 1.7435346839697508 >10 1.0769718086791258 8.276538138362431 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0023340491083932405 0.0 5 0.0 0.0 0.0 0.004668098216786481 0.0 6 0.0 0.0 0.0 0.004668098216786481 0.0 7 0.0 0.0 0.0 0.004668098216786481 0.0 8 0.0 0.0 0.0 0.004668098216786481 0.0 9 0.0 0.0 0.0 0.007002147325179722 0.0 10 0.0 0.0 0.0 0.007002147325179722 0.0 11 0.0 0.0 0.0 0.007002147325179722 0.0 12 0.0 0.0 0.0 0.007002147325179722 0.0 13 0.0 0.0 0.0 0.009336196433572962 0.0 14 0.0 0.0 0.0 0.009336196433572962 0.0 15 0.0 0.0 0.0 0.009336196433572962 0.0 16 0.0 0.0 0.0 0.016338343758752684 0.0 17 0.0 0.0 0.0 0.023340491083932408 0.0 18 0.0 0.0 0.0 0.028008589300718888 0.0 19 0.0 0.0 0.0 0.03967883484268509 0.0 20 0.0 0.0 0.0 0.046680982167864815 0.0 21 0.0 0.0 0.0 0.046680982167864815 0.0 22 0.0 0.0 0.0 0.05835122770983101 0.0 23 0.0 0.0 0.0 0.05835122770983101 0.0 24 0.0 0.0 0.0 0.06535337503501074 0.0 25 0.0 0.0 0.0 0.06535337503501074 0.0 26 0.0 0.0 0.0 0.07235552236019045 0.0 27 0.0 0.0 0.0 0.09102791522733639 0.0 28 0.0 0.0 0.0 0.10736625898608906 0.0 29 0.0 0.0 0.0 0.13537484828680796 0.0 30 0.0 0.0 0.0 0.18205583045467277 0.0 31 0.0 0.0 0.0 0.20539632153860518 0.0 32 0.0 0.0 0.0 0.22640276351414435 0.0 33 0.0 0.0 0.0 0.25674540192325646 0.0 34 0.0 0.0 0.0 0.2940901876575483 0.0 35 0.0 0.0 0.0 0.3734478573429185 0.0 36 0.0 0.0 0.0 0.4878162636541873 0.0 37 0.0 0.0 0.0 0.7095509289515451 0.0 38 0.0 0.0 0.0 1.1273457193539351 0.0 39 0.0 0.0 0.0 1.4821211838297077 0.0 40 0.0 0.0 0.0 2.007282233218187 0.0 41 0.0 0.0 0.0 2.6281392960507888 0.0 42 0.0 0.0 0.0 3.209317524040706 0.0 43 0.0 0.0 0.0 3.764821211838297 0.0 44 0.0 0.0 0.0 4.413686863971618 0.0 45 0.0 0.0 0.0 5.081224908972085 0.0 46 0.0 0.0 0.0 5.737092708430585 0.0 47 0.0 0.0 0.0 6.535337503501074 0.0 48 0.0 0.0 0.0 7.235552236019045 0.0 49 0.0 0.0 0.0 7.833068807767716 0.0 50 0.0 0.0 0.0 8.547287834936046 0.0 51 0.0 0.0 0.0 9.263840911212771 0.0 52 0.0 0.0 0.0 9.901036317804127 0.0 53 0.0 0.0 0.0 10.535897675287089 0.0 54 0.0 0.0 0.0 11.245448604238634 0.0 55 0.0 0.0 0.0 11.777611800952291 0.0 56 0.0 0.0 0.0 12.428811502194007 0.0 57 0.0 0.0 0.0 12.991317337316778 0.0 58 0.0 0.0 0.0 13.633180842124919 0.0 59 0.0 0.0 0.0 14.25403790495752 0.0 60 0.0 0.0 0.0 14.75119036504528 0.0 61 0.0 0.0 0.0 15.372047427877883 0.0 62 0.0 0.0 0.0 16.02558117822799 0.0 63 0.0 0.0 0.0 16.637102044627017 0.0 64 0.0 0.0 0.0 17.208944076183364 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAGTGC 15 0.002211136 69.99299 45 GGGAAGC 15 0.002211136 69.99299 6 AGTCTTG 15 0.002211136 69.99299 29 CTTGTCA 20 0.0069093597 52.494743 60 CGAAATC 20 0.0069093597 52.494743 27 ACTGATA 20 0.0069093597 52.494743 26 CTATAGG 20 0.0069093597 52.494743 51 GCTATCG 20 0.0069093597 52.494743 8 CAATTAC 20 0.0069093597 52.494743 34 AAGCGAG 20 0.0069093597 52.494743 9 GCATTTC 20 0.0069093597 52.494743 22 GTTCGAA 20 0.0069093597 52.494743 38 CGAGGTT 20 0.0069093597 52.494743 12 TGTTCGA 20 0.0069093597 52.494743 37 CGAATTC 20 0.0069093597 52.494743 41 TCGAGTG 20 0.0069093597 52.494743 44 GCGCTTG 20 0.0069093597 52.494743 57 TTCGAGT 20 0.0069093597 52.494743 43 TCGAATT 20 0.0069093597 52.494743 40 TATCATT 40 0.0023947668 34.996494 2 >>END_MODULE