FastQCFastQC Report
Wed 25 May 2016
SRR1780582_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780582_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42844
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT3420.7982447950704883Illumina PCR Primer Index 9 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT3240.7562319111194099No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC920.21473251797217813No Hit
CTTTACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC460.10736625898608906No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGGT150.002210258370.03
GAACTAG252.3650329E-455.9999961
TTAGGAA200.006906623552.534
TTGGCAT200.006906623552.529
CGTATCA200.006906623552.545
GATAAAG200.006906623552.516
GGATGGC200.006906623552.525
TAGAAGC200.006906623552.518
TGGCGGA200.006906623552.528
TTCCGCT200.006906623552.558
ATTCCGC200.006906623552.557
GGAAGAG200.006906623552.56
CAGATAA200.006906623552.514
CTCGGGC200.006906623552.539
GGGCGTA200.006906623552.542
CGGGCGT200.006906623552.541
ACAGAGT200.006906623552.53
GATGAGC200.006906623552.558
CAATGTA200.006906623552.516
GCGGATA200.006906623552.530
Produced by FastQC (version 0.11.5)