##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780582_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 42844 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.28412379796471 34.0 34.0 34.0 27.0 34.0 2 32.98727943235926 34.0 34.0 34.0 32.0 34.0 3 33.31278592101578 34.0 34.0 34.0 33.0 34.0 4 33.35914013630847 34.0 34.0 34.0 33.0 34.0 5 33.37641209971058 34.0 34.0 34.0 34.0 34.0 6 37.0310895341238 38.0 37.0 38.0 36.0 38.0 7 36.61994678368033 38.0 37.0 38.0 34.0 38.0 8 36.56726729530389 38.0 37.0 38.0 34.0 38.0 9 36.39695173186444 38.0 37.0 38.0 34.0 38.0 10-11 36.00245075156381 38.0 36.5 38.0 32.5 38.0 12-13 35.91323172439549 38.0 36.0 38.0 31.5 38.0 14-15 36.16674446830361 38.0 37.0 38.0 33.0 38.0 16-17 36.337188404444035 38.0 37.0 38.0 34.0 38.0 18-19 36.33744514984595 38.0 37.0 38.0 34.0 38.0 20-21 36.038278405377646 38.0 37.0 38.0 32.5 38.0 22-23 36.05915647465223 38.0 36.5 38.0 32.5 38.0 24-25 35.26915087293436 37.5 35.5 38.0 29.0 38.0 26-27 35.53846512930632 37.5 36.0 38.0 31.0 38.0 28-29 35.37211744935114 38.0 36.0 38.0 30.0 38.0 30-31 35.02093642050229 37.0 35.5 38.0 28.5 38.0 32-33 35.58633647651946 38.0 36.0 38.0 31.0 38.0 34-35 35.603118289608815 38.0 36.5 38.0 31.5 38.0 36-37 35.82317243954813 38.0 36.5 38.0 32.0 38.0 38-39 35.440143310615255 38.0 36.5 38.0 29.5 38.0 40-41 34.82333582298571 37.0 34.5 38.0 27.0 38.0 42-43 35.295969097189804 37.0 35.5 38.0 29.0 38.0 44-45 35.507667351321075 38.0 36.0 38.0 31.0 38.0 46-47 35.82054663430119 38.0 36.5 38.0 31.5 38.0 48-49 35.597831668378305 38.0 36.0 38.0 31.0 38.0 50-51 35.794638689198024 38.0 36.5 38.0 32.0 38.0 52-53 35.70128839510783 38.0 36.0 38.0 31.5 38.0 54-55 35.86371487256092 38.0 37.0 38.0 32.0 38.0 56-57 35.94681869106526 38.0 37.0 38.0 33.0 38.0 58-59 36.043926804219964 38.0 37.0 38.0 33.5 38.0 60-61 35.662018018859115 38.0 36.5 38.0 31.5 38.0 62-63 35.93868452992251 38.0 36.5 38.0 33.0 38.0 64-65 35.95629493044534 38.0 37.0 38.0 33.0 38.0 66-67 35.216284660629256 38.0 36.0 38.0 29.0 38.0 68-69 34.563789562132385 37.0 34.5 38.0 26.0 38.0 70-71 34.500466809821674 37.0 34.0 38.0 25.5 38.0 72-73 34.22548081411633 37.0 34.0 38.0 24.5 38.0 74-75 35.03321351881243 37.0 35.5 38.0 27.5 38.0 76 34.478550088693865 37.0 34.0 38.0 25.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.05161515482625845 1101 2 0.5338209765035415 1101 3 0.45992683830217373 1101 4 0.31967929366277303 1101 5 0.3011562746078482 1101 6 -1.2622733457354371 1101 7 -0.35760419148765266 1101 8 0.8251312487124736 1101 9 0.5523096457705989 1101 10-11 0.4128370390946259 1101 12-13 0.8270119888806136 1101 14-15 0.941085207264031 1101 16-17 -0.06052209232990435 1101 18-19 -0.4804749298730542 1101 20-21 -0.10373323915834476 1101 22-23 0.8386131065130797 1101 24-25 -1.4789246254624189 1101 26-27 0.2313651725215209 1101 28-29 0.8834373297219642 1101 30-31 1.3415916933224494 1101 32-33 0.022354401701470294 1101 34-35 0.9796331549610713 1101 36-37 0.5371112441141719 1101 38-39 0.2712032976356866 1101 40-41 1.798832305320751 1101 42-43 1.0155155037825025 1101 44-45 0.2267775616883867 1101 46-47 0.5327866349213437 1101 48-49 -1.5569833720033657 1101 50-51 -2.817455208553106 1101 52-53 -2.340792630124561 1101 54-55 -0.8951792190898473 1101 56-57 -1.4000525274617814 1101 58-59 -1.8479636635300452 1101 60-61 -2.508021983445076 1101 62-63 -0.8877890080201425 1101 64-65 -1.3321910515242408 1101 66-67 -1.9036842688173152 1101 68-69 -1.7974946101739633 1101 70-71 -0.3383720722555452 1101 72-73 -1.8265086433159965 1101 74-75 -0.12075723237867919 1101 76 -0.21104166956350667 1102 1 -0.28738484517374374 1102 2 0.5704876431702104 1102 3 0.5469268383021699 1102 4 0.6140126269961073 1102 5 0.19582294127452116 1102 6 0.47705998759789736 1102 7 0.28072914184568276 1102 8 0.7174645820458068 1102 9 0.8773096457706018 1102 10-11 0.8041703724279614 1102 12-13 1.0830119888806067 1102 14-15 0.1320852072640264 1102 16-17 0.3161445743367679 1102 18-19 -0.3888082632063856 1102 20-21 0.42010009417498395 1102 22-23 0.3396131065130845 1102 24-25 -4.270757958795755 1102 26-27 -1.0799681608118163 1102 28-29 0.286603996388628 1102 30-31 1.8159250266557834 1102 32-33 0.8355210683681307 1102 34-35 1.1977998216277328 1102 36-37 -2.0802220892191627 1102 38-39 0.4908699643023553 1102 40-41 1.7056656386540823 1102 42-43 1.5638488371158346 1102 44-45 1.6114442283550545 1102 46-47 1.4249533015880047 1102 48-49 -2.584816705336692 1102 50-51 -0.545955208553103 1102 52-53 0.1745407032087698 1102 54-55 0.7783207809101569 1102 56-57 0.5781141392048852 1102 58-59 0.7480363364699514 1102 60-61 -5.4341886501117465 1102 62-63 -2.2469556746868093 1102 64-65 0.44064228180909026 1102 66-67 1.4679823978493545 1102 68-69 -0.4468279435072944 1102 70-71 -1.5982054055888781 1102 72-73 -6.724675309982665 1102 74-75 -2.048257232378681 1102 76 -0.028041669563506844 1103 1 1.5223090323772794 1103 2 0.8429366227620463 1103 3 0.03723296075114746 1103 4 0.5604411984246767 1103 5 0.40466647868948513 1103 6 0.04814842297204791 1103 7 1.0053890057912582 1103 8 0.7084509766036362 1103 9 0.6230579450903306 1103 10-11 1.0255989438565294 1103 12-13 0.21362423377856743 1103 14-15 -0.22454744579719232 1103 16-17 0.4953962750170433 1103 18-19 0.8317699680861352 1103 20-21 -0.24103936160732076 1103 22-23 1.0554294330436917 1103 24-25 -3.1796865302243305 1103 26-27 -0.022485167614533452 1103 28-29 0.17342372427978603 1103 30-31 1.2446665232544234 1103 32-33 1.6254020207490854 1103 34-35 0.6749256719678769 1103 36-37 -1.0632152864980782 1103 38-39 -0.04229330100377382 1103 40-41 0.5239479515792596 1103 42-43 -0.14244367989096673 1103 44-45 1.6947775616883902 1103 46-47 1.4670451383226961 1103 48-49 -1.3849017393503047 1103 50-51 0.32572846491628127 1103 52-53 0.7449488664740826 1103 54-55 0.45306567886934346 1103 56-57 0.8906141392048781 1103 58-59 -0.35196366353005004 1103 60-61 -3.86913762970358 1103 62-63 -1.2671087359113002 1103 64-65 0.6538225539179336 1103 66-67 0.19944498288336376 1103 68-69 0.9402468864246742 1103 70-71 1.1962673835267665 1103 72-73 -4.7977195276697415 1103 74-75 -1.5968116541473876 1103 76 -2.790796771604324 1104 1 0.9265040437151413 1104 2 0.7274320876146589 1104 3 0.6469268383021713 1104 4 -0.21932070633722844 1104 5 -0.3777881698365917 1104 6 0.2853933209312274 1104 7 0.9807291418456856 1104 8 0.7563534709346982 1104 9 1.4398096457706018 1104 10-11 -0.7263851831275971 1104 12-13 0.20037309999172237 1104 14-15 0.3466685405973635 1104 16-17 0.35572790767010076 1104 18-19 0.6257750701269487 1104 20-21 -0.3680943502694589 1104 22-23 -0.11733133793136119 1104 24-25 0.46465870787090324 1104 26-27 1.2040596169659636 1104 28-29 1.4366039963886337 1104 30-31 0.9048139155446719 1104 32-33 0.32927106836812925 1104 34-35 0.27002204384995565 1104 36-37 1.1246390218919373 1104 38-39 1.3068421865245696 1104 40-41 1.9014989719874151 1104 42-43 0.9048210593380546 1104 44-45 1.2572775616883902 1104 46-47 1.0812033015880118 1104 48-49 0.13323885021885928 1104 50-51 0.9221003470024485 1104 52-53 1.050929592097667 1104 54-55 0.03873744757682829 1104 56-57 0.4426974725382138 1104 58-59 -0.07418588575227147 1104 60-61 0.4616446832215928 1104 62-63 0.36901654753541635 1104 64-65 0.8420311706979788 1104 66-67 0.9908990645160216 1104 68-69 1.8642831676038156 1104 70-71 2.351100149966676 1104 72-73 1.6496302455728866 1104 74-75 0.29271498984353883 1104 76 0.14556944154760743 1105 1 1.3957232629343608 1105 2 0.7024696251521902 1105 3 0.5624673788427117 1105 4 -0.2226990847156074 1105 5 -0.46543831998674534 1105 6 0.29778070831861925 1105 7 0.8602336463501885 1105 8 -0.5638417242604987 1105 9 0.9212285646895211 1105 10-11 0.8201613634189471 1105 12-13 -0.624420443551827 1105 14-15 0.06992304510186642 1105 16-17 0.11164006983226216 1105 18-19 0.8783088539107311 1105 20-21 0.5107532473281395 1105 22-23 0.024748241648211433 1105 24-25 0.6102105096727044 1105 26-27 0.6709327400890857 1105 28-29 1.0452751675598009 1105 30-31 1.3650241257548856 1105 32-33 0.8441922395393036 1105 34-35 -1.0090695477416318 1105 36-37 0.44567881168173074 1105 38-39 0.11136545979784529 1105 40-41 -0.3563839108954596 1105 42-43 0.23862361189060977 1105 44-45 1.2251829670937937 1105 46-47 1.351192040326744 1105 48-49 -0.3558752638952498 1105 50-51 1.2280312779333755 1105 52-53 0.7053965590646243 1105 54-55 0.8847396998290762 1105 56-57 0.4198934184841647 1105 58-59 0.519657958091571 1105 60-61 1.1497077462846477 1105 62-63 -0.40359981883094775 1105 64-65 1.0589981376649504 1105 66-67 0.994558974425928 1105 68-69 0.8601540384746826 1105 70-71 1.1111414412579705 1105 72-73 1.033545410738057 1105 74-75 -0.5485950702165212 1105 76 0.5293907628689283 1106 1 -0.9681100360134387 1106 2 0.4125996024577461 1106 3 0.34444592227163895 1106 4 0.12896173641086506 1106 5 0.13297306850098067 1106 6 0.18456634892105228 1106 7 -0.5692072449227652 1106 8 0.607604531155225 1106 9 0.2561264396637313 1106 10-11 0.5406843673388977 1106 12-13 1.3280501568195433 1106 14-15 -0.4558918919726125 1106 16-17 0.5277221824792662 1106 18-19 0.5973876655467976 1106 20-21 0.5166331730553964 1106 22-23 0.9420940225436141 1106 24-25 -0.29782538882120235 1106 26-27 1.1987850198497654 1106 28-29 0.8601090854471565 1106 30-31 0.9927061971392419 1106 32-33 0.31551470704497575 1106 34-35 1.5848545290068685 1106 36-37 1.1882257479309573 1106 38-39 0.4167605495440796 1106 40-41 1.7119315419619738 1106 42-43 0.8700193205763895 1106 44-45 1.3550829051998434 1106 46-47 1.4505576272877576 1106 48-49 1.6896425821951127 1106 50-51 0.32074326472933734 1106 52-53 0.540062331707496 1106 54-55 0.24921772747504178 1106 56-57 0.452955106125998 1106 58-59 1.0717004586073529 1106 60-61 0.9963138944175114 1106 62-63 0.9902262591554276 1106 64-65 -0.5748475400738613 1106 66-67 1.1587584792742902 1106 68-69 2.3040626417344328 1106 70-71 0.7273989201108719 1106 72-73 0.2523921200427779 1106 74-75 -0.2355663926840279 1106 76 0.17711100219221976 1107 1 1.416985784196882 1107 2 -0.4776475549649888 1107 3 0.06563313200846466 1107 4 0.11721775520123145 1107 5 0.31447095992253793 1107 6 0.18953085006875625 1107 7 0.8930251791417234 1107 8 -0.12933028974906335 1107 9 -0.6742637808028249 1107 10-11 0.8201377383953172 1107 12-13 0.5816133874820082 1107 14-15 0.31512716530598794 1107 16-17 0.7624149706071606 1107 18-19 0.7579866085884888 1107 20-21 1.1746164111913089 1107 22-23 -0.08678549488551823 1107 24-25 0.6271732766354745 1107 26-27 -0.23579566663931928 1107 28-29 1.071073693358322 1107 30-31 0.8303189660497239 1107 32-33 1.226634121981185 1107 34-35 0.2513254626533765 1107 36-37 1.4873769783798991 1107 38-39 0.7096928081251903 1107 40-41 0.8886784591669041 1107 42-43 0.7345364828034775 1107 44-45 0.6668055337163636 1107 46-47 1.3382691524038606 1107 48-49 1.0543732713532776 1107 50-51 1.3840273089294115 1107 52-53 0.8772213558894251 1107 54-55 0.8757858158751972 1107 56-57 0.9022691508598939 1107 58-59 0.5326517210853368 1107 60-61 1.4339640305409347 1107 62-63 1.077665537434406 1107 64-65 1.2037250323918371 1107 66-67 0.7294625843295393 1107 68-69 1.4326592359798838 1107 70-71 0.632592962709488 1107 72-73 1.6980228252154674 1107 74-75 -0.42928870091014915 1107 76 0.7228324563106199 1108 1 -0.4659059719343084 1108 2 0.5045252018556567 1108 3 0.43389866928808374 1108 4 0.5993412654937558 1108 5 0.1784520492557391 1108 6 0.12752947116597113 1108 7 0.19845214654051802 1108 8 -0.23171382171005916 1108 9 -0.08571852324348583 1108 10-11 0.8497102785312478 1108 12-13 1.190054242401736 1108 14-15 0.959197883320364 1108 16-17 -0.22657843035807446 1108 18-19 0.3955814081551168 1108 20-21 1.3763794368979916 1108 22-23 0.31743000792153 1108 24-25 1.1018359379178548 1108 26-27 -0.2804376443798873 1108 28-29 -0.9897457688695894 1108 30-31 0.9484367637449864 1108 32-33 1.3338191904338643 1108 34-35 0.98588667608783 1108 36-37 1.4678999765085337 1108 38-39 1.484414565241316 1108 40-41 1.6890576574334233 1108 42-43 0.009036630543057811 1108 44-45 1.0081578433785339 1108 46-47 1.134166916611484 1108 48-49 1.0412983181374855 1108 50-51 1.2644321153905551 1108 52-53 0.35599610226980616 1108 54-55 0.06265176682565254 1108 56-57 0.966905219017093 1108 58-59 1.0283180266107976 1108 60-61 1.4446259038788654 1108 62-63 0.8984081750784512 1108 64-65 1.0564286667856138 1108 66-67 1.302372069210854 1108 68-69 1.9456603194035011 1108 70-71 -0.38828756521328955 1108 72-73 -0.024029770076559487 1108 74-75 -0.4466727253364269 1108 76 1.0758315698731096 1109 1 -0.9286969019113336 1109 2 0.450984097070922 1109 3 0.6513594624156482 1109 4 0.2159629816060331 1109 5 0.22490095545891364 1109 6 -0.19776270743756186 1109 7 -0.2222850425514764 1109 8 0.8275709650245346 1109 9 1.2487635464798217 1109 10-11 1.1243831383854044 1109 12-13 0.9028701449089738 1109 14-15 0.7932554200299862 1109 16-17 0.976073652350955 1109 18-19 1.120234289985106 1109 20-21 1.3398695977210906 1109 22-23 0.4685138157329405 1109 24-25 0.037132112126222694 1109 26-27 0.6601027611740022 1109 28-29 -0.5797967128312322 1109 30-31 0.6746129699181935 1109 32-33 0.8680565293610414 1109 34-35 0.30178918332986626 1109 36-37 1.0970828753198418 1109 38-39 0.9793451416073182 1109 40-41 0.39440677340586205 1109 42-43 1.1526786243498748 1109 44-45 1.1947775616883902 1109 46-47 1.2534107483965187 1109 48-49 0.7337116634576333 1109 50-51 0.16087103258164603 1109 52-53 -0.09815433225222137 1109 54-55 0.0855016319739903 1109 56-57 0.5324581108360888 1109 58-59 0.5593838542004477 1109 60-61 1.331325534285419 1109 62-63 0.1208634742493615 1109 64-65 1.1552699413835583 1109 66-67 0.9117767240904868 1109 68-69 -0.04115418464204623 1109 70-71 0.5302520412196259 1109 72-73 0.5202714985279755 1109 74-75 -0.14160829620846727 1109 76 0.6607881176705348 1110 1 -1.7045723451737445 1110 2 -1.1805540234964553 1110 3 -0.43119816169782865 1110 4 0.030679293662771556 1110 5 0.5900937746078512 1110 6 -0.11564834573543692 1110 7 0.7723958085123499 1110 8 0.5263187487124767 1110 9 1.3226221457706018 1110 10-11 -0.7229129609053757 1110 12-13 0.6283244888806081 1110 14-15 1.1344289572640278 1110 16-17 0.6135404076701008 1110 18-19 -0.04219367987305134 1110 20-21 0.7430167608416554 1110 22-23 -0.5986681434869183 1110 24-25 -1.2514871254624254 1110 26-27 1.2088339225215208 1110 28-29 -0.09725017027803773 1110 30-31 0.12009169332245051 1110 32-33 0.47510440170146495 1110 34-35 0.9826956549610699 1110 36-37 1.3038924941141659 1110 38-39 0.9452970476356839 1110 40-41 -1.1909489446792492 1110 42-43 0.5250467537824974 1110 44-45 1.3861838116883902 1110 46-47 0.7778178849213404 1110 48-49 1.6219541279966379 1110 50-51 0.29623229144689134 1110 52-53 0.5826136198754384 1110 54-55 -0.4130854690898431 1110 56-57 0.6419162225382138 1110 58-59 -0.32461991353005004 1110 60-61 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52-53 -0.7425135603571178 2116 54-55 -1.0429001493223993 2116 56-57 -0.7120990390896935 2116 58-59 -2.9740566867858647 2116 60-61 -2.2253708206543763 2116 62-63 0.2008156431426542 2116 64-65 -2.33949337710564 2116 66-67 -2.129498222305685 2116 68-69 -2.3146574008716385 2116 70-71 0.8127442068142159 2116 72-73 -0.26469468982762834 2116 74-75 0.12063811645852951 2116 76 -2.1704835300286263 2117 1 0.8004356676467665 2117 2 0.36151328419585127 2117 3 -0.42038085400552205 2117 4 -0.17765403967056415 2117 5 0.20607935153093138 2117 6 -0.3652477047097946 2117 7 0.7788060649226054 2117 8 0.44759278717401685 2117 9 -1.8502544567935004 2117 10-11 -1.9240347557771713 2117 12-13 -1.1887828829142677 2117 14-15 0.11990572008453881 2117 16-17 -1.672316964124768 2117 18-19 -2.1202185196166425 2117 20-21 -0.43727170069680454 2117 22-23 -0.4661561242561447 2117 24-25 -3.281334881872681 2117 26-27 -3.787660468504118 2117 28-29 -2.3374344651498333 2117 30-31 -0.6026647169339583 2117 32-33 -3.442363547016484 2117 34-35 0.2513254626533765 2117 36-37 -0.8750938840909583 2117 38-39 -0.4225915741591919 2117 40-41 -0.6433728228843734 2117 42-43 -0.29576654749955367 2117 44-45 -1.38214551523469 2117 46-47 -2.095880031745324 2117 48-49 -2.2748808079008 2117 50-51 -1.9090962341941307 2117 52-53 0.33351506218313176 2117 54-55 -0.8514869113975365 2117 56-57 -0.6222063736156329 2117 58-59 -1.85196366353005 2117 60-61 -1.3556630090861006 2117 62-63 -0.4549684951996298 2117 64-65 -1.9507038720370602 2117 66-67 -1.3031714483044965 2117 68-69 -0.22759717427652504 2117 70-71 -0.3703207902042607 2117 72-73 0.4410298182224679 2117 74-75 0.4238581522367042 2117 76 0.7123429458211064 2118 1 -2.3918720246609233 2118 2 -0.6384867158041487 2118 3 -2.215252648877314 2118 4 -0.28021814223466635 2118 5 0.20607935153093138 2118 6 -0.19858103804313032 2118 7 0.2788060649226054 2118 8 -0.39856105897982985 2118 9 -2.9271775337165806 2118 10-11 -1.4753168070592224 2118 12-13 -1.618270062401443 2118 14-15 0.08785443803326132 2118 16-17 -2.1402656820734904 2118 18-19 -0.47278262218074474 2118 20-21 -1.3603486237737314 2118 22-23 0.34794643984641027 2118 24-25 -0.03774513828293635 2118 26-27 -5.479968160811815 2118 28-29 -1.8053831830985487 2118 30-31 -1.5898442041134473 2118 32-33 -0.8205686752216153 2118 34-35 -1.3448283835004702 2118 36-37 -3.772529781526856 2118 38-39 -1.6790018305694474 2118 40-41 -1.9574753869869426 2118 42-43 -0.23166398339699157 2118 44-45 -3.7218891049782776 2118 46-47 -0.5766492625145574 2118 48-49 -1.2684705514905446 2118 50-51 -2.3706346957325977 2118 52-53 -3.781869553201485 2118 54-55 -2.5373843472949673 2118 56-57 -1.2311807325899906 2118 58-59 -3.4160662276326192 2118 60-61 -2.5928424962655896 2118 62-63 -2.9998402900714254 2118 64-65 -0.6814731028062937 2118 66-67 -1.508299653432701 2118 68-69 -2.5673407640201162 2118 70-71 -2.3703207902042607 2118 72-73 0.4923118695045119 2118 74-75 -1.2363982580197046 2118 76 -0.6850929516147914 2119 1 -0.3970002297891284 2119 2 -1.70002517734261 2119 3 -0.7973039309285994 2119 4 -0.6981668601833846 2119 5 -0.724689879238305 2119 6 -1.3755041149662048 2119 7 -0.7237580376414954 2119 8 0.16297740255863147 2119 9 -2.447690354229401 2119 10-11 -1.440701422443837 2119 12-13 -0.7400649341963117 2119 14-15 -1.1506071004282816 2119 16-17 -1.4620605538683549 2119 18-19 -0.09585954525766738 2119 20-21 -1.944964008389114 2119 22-23 -0.45461766271768767 2119 24-25 0.26353691299910764 2119 26-27 -0.5735579044015537 2119 28-29 -0.7694857472011094 2119 30-31 1.049899385630141 2119 32-33 0.26020055554761967 2119 34-35 0.48978700111491236 2119 36-37 -0.262273371270453 2119 38-39 -1.3238736254412373 2119 40-41 -2.095936925448477 2119 42-43 -0.021407573140578506 2119 44-45 0.5717006386114676 2119 46-47 -0.463828749694045 2119 48-49 -0.973598756618749 2119 50-51 0.6203909452930461 2119 52-53 -0.33956186089379514 2119 54-55 -1.7361022960129233 2119 56-57 -2.1145140659233235 2119 58-59 -0.9211944327608208 2119 60-61 0.21228570886261622 2119 62-63 0.10913406890293942 2119 64-65 -1.5263448976780865 2119 66-67 -0.36983811497115937 2119 68-69 0.6480438513644984 2119 70-71 -1.0562182261016986 2119 72-73 0.9602605874532273 2119 74-75 -1.4146033862248402 2119 76 -1.8184262849481243 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 5.0 23 14.0 24 28.0 25 55.0 26 82.0 27 186.0 28 336.0 29 493.0 30 875.0 31 1367.0 32 2092.0 33 3167.0 34 4814.0 35 6836.0 36 10632.0 37 11861.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.9817010549902 24.901969937447486 11.91998879656428 27.196340210998038 2 17.498366165624123 20.156848100084026 38.47213145364579 23.872654280646064 3 17.050228736812624 26.75287088040332 30.195593315283354 26.0013070675007 4 12.90729156941462 19.22556250583512 39.80720754364672 28.05993838110354 5 11.976005975165718 38.920268882457286 36.20343572028755 12.900289422089442 6 27.05396321538605 39.31472318177575 18.702735505555037 14.928578097283166 7 26.486789282046498 30.00420128839511 24.376808888059003 19.132200541499394 8 22.74530856129213 36.75660535897675 20.364578470731022 20.13350760900009 9 23.240126972271497 15.360377182335917 23.319484641956866 38.080011203435724 10-11 24.500513490803847 26.265054616749133 27.799691905517694 21.434739986929323 12-13 24.19241900849594 23.32181869106526 26.957100177387733 25.52866212305107 14-15 21.728830174586875 25.69437960974699 23.807300905611054 28.769489310055086 16-17 22.46522266828494 27.92339650826253 25.82275231070862 23.78862851274391 18-19 21.843198580898143 27.517271963402113 26.971104472038093 23.668424983661655 20-21 21.033283540285687 27.872047427877884 26.449444496312204 24.64522453552423 22-23 22.454719447297173 27.828867519372608 27.06096536271123 22.65544767061899 24-25 22.36952665484082 28.326019979460366 26.726029315656803 22.578424050042013 26-27 21.376388759219495 28.18714405751097 28.038931939128 22.397535244141537 28-29 21.680982167864812 26.85673606572682 28.932872747642612 22.529409018765755 30-31 21.410232471291195 27.405237606199233 28.854682102511436 22.329847819998133 32-33 21.08113154700775 28.572262160395855 27.544113528148635 22.802492764447763 34-35 21.427737839604145 28.934039772196808 28.18597703295677 21.452245355242276 36-37 22.312342451685183 27.22318177574456 27.6584819344599 22.805993838110354 38-39 21.55377649145738 27.072635608253197 28.09961721594622 23.273970684343197 40-41 21.217673419848754 26.588320418261603 28.90369713378769 23.29030902810195 42-43 22.201475119036505 26.33974418821772 28.96788348426851 22.490897208477268 44-45 20.55246942395668 26.8310615255345 28.63994958453926 23.976519465969563 46-47 21.807020819718048 25.6897115115302 28.960881336943327 23.54238633180842 48-49 20.516291662776585 25.925450471477916 29.468537018018857 24.089720847726635 50-51 19.908271870040146 26.38875921949398 29.466202968910466 24.23676594155541 52-53 19.631687050695547 26.26972271496592 30.861964335729624 23.236625898608906 54-55 18.5766968537018 26.624498179441698 30.27378396041453 24.525021006441975 56-57 20.124171412566522 26.33040799178415 29.103258332555313 24.442162263094016 58-59 19.15787508169172 27.4857623004388 28.962048361497526 24.394314256371956 60-61 20.05881803753151 28.454392680421996 27.464755858463263 24.022033423583235 62-63 18.4051442442349 29.037904957520304 27.601297731304264 24.955653066940528 64-65 19.199887965642798 29.770796377555786 26.47745308561292 24.551862571188497 66-67 19.21622630940155 28.98072075436467 26.407431612361126 25.395621323872653 68-69 19.148538885258144 28.840677807861077 26.248716272990386 25.762067033890396 70-71 19.59550928951545 28.676127345719355 25.99430492017552 25.734058444589675 72-73 19.24423489870227 27.71800018672393 26.2767248622911 26.7610400522827 74-75 19.411119409952384 27.71333208850714 26.300065353375036 26.575483148165436 76 19.73438521146485 28.057604331995144 26.234711978340027 25.97329847819998 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 1.0 18 1.0 19 2.5 20 4.0 21 4.0 22 7.0 23 21.0 24 33.0 25 34.0 26 75.5 27 145.5 28 200.5 29 227.0 30 264.0 31 398.5 32 564.0 33 632.0 34 752.5 35 969.0 36 1194.5 37 1324.0 38 1537.5 39 2085.5 40 2732.0 41 3107.5 42 3171.0 43 3221.5 44 3290.5 45 3379.5 46 3450.0 47 3460.0 48 3267.5 49 2800.5 50 2536.0 51 2333.0 52 1882.5 53 1480.5 54 1326.0 55 1199.5 56 875.0 57 595.0 58 513.0 59 392.0 60 233.5 61 157.0 62 118.0 63 89.0 64 47.5 65 24.5 66 9.0 67 4.0 68 3.5 69 3.0 70 2.5 71 2.0 72 1.5 73 0.5 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 42844.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.5544767061899 #Duplication Level Percentage of deduplicated Percentage of total 1 68.20898225280695 35.16478386705256 2 13.527707352408546 13.948277471758006 3 6.90420137631293 10.678274670899075 4 3.8074972835929013 7.851741200634861 5 2.1821803694313657 5.625058351227709 6 1.5528793915248098 4.803473065073289 7 1.0503440782325244 3.7904957520306226 8 0.7424846070264397 3.0622724302119315 9 0.48442593263310396 2.2476892913826907 >10 1.5257153205360376 11.058724675567174 >50 0.004527345164795364 0.21473251797217813 >100 0.009054690329590729 1.554476706189898 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT 342 0.7982447950704883 Illumina PCR Primer Index 9 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT 324 0.7562319111194099 No Hit ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 92 0.21473251797217813 No Hit CTTTACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC 46 0.10736625898608906 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0023340491083932405 0.0 2 0.0 0.0 0.0 0.0023340491083932405 0.0 3 0.0 0.0 0.0 0.0023340491083932405 0.0 4 0.0 0.0 0.0 0.004668098216786481 0.0 5 0.0 0.0 0.0 0.007002147325179722 0.0 6 0.0 0.0 0.0 0.007002147325179722 0.0 7 0.0 0.0 0.0 0.007002147325179722 0.0 8 0.0 0.0 0.0 0.007002147325179722 0.0 9 0.0 0.0 0.0 0.011670245541966204 0.0 10 0.0 0.0 0.0 0.011670245541966204 0.0 11 0.0 0.0 0.0 0.011670245541966204 0.0 12 0.0 0.0 0.0 0.011670245541966204 0.0 13 0.0 0.0 0.0 0.014004294650359444 0.0 14 0.0 0.0 0.0 0.014004294650359444 0.0 15 0.0 0.0 0.0 0.014004294650359444 0.0 16 0.0 0.0 0.0 0.021006441975539164 0.0 17 0.0 0.0 0.0 0.025674540192325648 0.0 18 0.0 0.0 0.0 0.030342638409112128 0.0 19 0.0 0.0 0.0 0.04201288395107833 0.0 20 0.0 0.0 0.0 0.046680982167864815 0.0 21 0.0 0.0 0.0 0.046680982167864815 0.0 22 0.0 0.0 0.0 0.04901503127625805 0.0 23 0.0 0.0 0.0 0.04901503127625805 0.0 24 0.0 0.0 0.0 0.04901503127625805 0.0 25 0.0 0.0 0.0 0.04901503127625805 0.0 26 0.0 0.0 0.0 0.05368312949304453 0.0 27 0.0 0.0 0.0 0.06535337503501074 0.0 28 0.0 0.0 0.0 0.07935766968537018 0.0 29 0.0 0.0 0.0 0.10503220987769582 0.0 30 0.0 0.0 0.0 0.15638129026234712 0.0 31 0.0 0.0 0.0 0.1727196340210998 0.0 32 0.0 0.0 0.0 0.19372607599663896 0.0 33 0.0 0.0 0.0 0.2240687144057511 0.0 34 0.0 0.0 0.0 0.25674540192325646 0.0 35 0.0 0.0 0.0 0.32910092428344695 0.0 36 0.0 0.0 0.0 0.427130986835963 0.0 37 0.0 0.0 0.0 0.6278592101577817 0.0 38 0.0 0.0 0.0 1.0456540005601718 0.0 39 0.0 0.0 0.0 1.4284380543366633 0.0 40 0.0 0.0 0.0 1.9185883670992439 0.0 41 0.0 0.0 0.0 2.5371113808234527 0.0 42 0.0 0.0 0.0 3.092615068621044 0.0 43 0.0 0.0 0.0 3.6411166090934555 0.0 44 0.0 0.0 0.0 4.268975819251237 0.0 45 0.0 0.0 0.0 4.915507422276165 0.0 46 0.0 0.0 0.0 5.557370927084306 0.0 47 0.0 0.0 0.0 6.357949771263187 0.0 48 0.0 0.0 0.0 7.039492110914014 0.0 49 0.0 0.0 0.0 7.63467463355429 0.0 50 0.0 0.0 0.0 8.362897955372981 0.0 51 0.0 0.0 0.0 9.06778078610774 0.0 52 0.0 0.0 0.0 9.718980487349453 0.0 53 0.0 0.0 0.0 10.351507795724022 0.0 54 0.0 0.0 0.0 11.06339277378396 0.0 55 0.0 0.0 0.0 11.600224068714406 0.0 56 0.0 0.0 0.0 12.209410886005042 0.0 57 0.0 0.0 0.0 12.776584819344599 0.0 58 0.0 0.0 0.0 13.41844832415274 0.0 59 0.0 0.0 0.0 14.048641583418915 0.0 60 0.0 0.0 0.0 14.543459994398281 0.0 61 0.0 0.0 0.0 15.136308467930165 0.0 62 0.0 0.0 0.0 15.75949957987116 0.0 63 0.0 0.0 0.0 16.338343758752686 0.0 64 0.0 0.0 0.0 16.877509102791524 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTAGGT 15 0.0022102583 70.0 3 GAACTAG 25 2.3650329E-4 55.999996 1 TTAGGAA 20 0.0069066235 52.5 34 TTGGCAT 20 0.0069066235 52.5 29 CGTATCA 20 0.0069066235 52.5 45 GATAAAG 20 0.0069066235 52.5 16 GGATGGC 20 0.0069066235 52.5 25 TAGAAGC 20 0.0069066235 52.5 18 TGGCGGA 20 0.0069066235 52.5 28 TTCCGCT 20 0.0069066235 52.5 58 ATTCCGC 20 0.0069066235 52.5 57 GGAAGAG 20 0.0069066235 52.5 6 CAGATAA 20 0.0069066235 52.5 14 CTCGGGC 20 0.0069066235 52.5 39 GGGCGTA 20 0.0069066235 52.5 42 CGGGCGT 20 0.0069066235 52.5 41 ACAGAGT 20 0.0069066235 52.5 3 GATGAGC 20 0.0069066235 52.5 58 CAATGTA 20 0.0069066235 52.5 16 GCGGATA 20 0.0069066235 52.5 30 >>END_MODULE