FastQCFastQC Report
Wed 25 May 2016
SRR1780581_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780581_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences603723
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAACTT207.85802E-443.9948626
GTTAAGC357.356937E-637.659913
CGGTCGT301.2968166E-436.68367810
CGCTTGA250.002351294735.20172522
CGAGAAC401.8179568E-533.00161722
GGCACTC300.005718143729.35912340
ACCTCGA300.005718143729.35912339
TGGCACT300.005718143729.35912339
CGAAACT300.005743710329.33233825
ACTTGTG300.00574603929.32990629
TTTACGA300.005757693729.31774933
CTGGTAC300.005783429.291043
CCGGTCG454.10643E-529.2910399
CGAACGA554.9186856E-628.02694314
CCGAACG554.921865E-628.02461813
CGTTGAG554.950549E-628.00369630
GCCGTTG554.960144E-627.99672928
TGGTACA407.0875464E-427.4603484
AGAACGG508.2802566E-526.42540624
AAGTTGG450.001390264424.46593740
Produced by FastQC (version 0.11.5)