FastQCFastQC Report
Wed 25 May 2016
SRR1780581_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780581_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences603723
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT90611.500853868413163No Hit
ATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTCTT35820.59331845896214TruSeq Adapter, Index 9 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC13320.22063098473969023No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC11480.19015343129216544No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG8610.14261507346912408No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAC207.8558386E-443.9973522
CGTGCGA501.3460522E-939.60089510
CCGTCTT4350.036.92037244
GTGCCAA250.002341108235.2328951
ACTGTAC250.00235160235.20079442
GCCGTCT4650.034.5384143
CGAGAAT607.994458E-933.00074414
TTATCAA756.184564E-1132.26472532
CGGGACG353.2171057E-431.42667623
ATGATTC1350.030.96366340
GCGCGGT300.005744454529.33156633
CACAAGT300.005744454529.33156620
CTTTTGA554.954778E-628.00063315
TGAAGTT953.092282E-1127.790139
GAGTTCC407.027099E-427.50062215
TCCGGTC407.0305023E-427.4983438
TGGAATG655.926049E-727.0752921
TTCGGTA655.926049E-727.0752926
TGCCGTC6050.026.54605542
CATAAGT508.333357E-526.4005973
Produced by FastQC (version 0.11.5)