FastQCFastQC Report
Wed 25 May 2016
SRR1780580_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780580_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16370
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT3682.24801466096518RNA PCR Primer, Index 30 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT1731.056811240073305No Hit
CTTTACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC630.38485033598045204No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC550.3359804520464264No Hit
TATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCT320.19547953573610263RNA PCR Primer, Index 30 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCGTCTT250.1527183872938302No Hit
CTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC190.11606597434331094No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCCTT150.002185777870.034
ATGTCCT150.002185777870.033
CCACCAT200.006830707752.520
GGATCTC1151.2478267E-930.43478432
GCCGGAT1301.382432E-1029.61538329
ATCTCGT1201.9663275E-929.16666634
CGGATCT1201.9663275E-929.16666631
TCTCGTA1201.9663275E-929.16666635
GATCTCG1201.9663275E-929.16666633
CCGGATC1304.62569E-926.92307730
TCTGCTT1454.9294613E-1026.55172550
GTATGCC1507.3123374E-1025.66666642
TGCCGGA1507.3123374E-1025.66666628
TATGCCG1507.3123374E-1025.66666643
ACATTGC1507.3123374E-1025.66666624
ATTGCCG1507.3123374E-1025.66666626
CGTATGC1507.3123374E-1025.66666638
CTGCTTG1507.3123374E-1025.66666651
TCGTATG1507.3123374E-1025.66666637
TTGAAAA1507.3123374E-1025.66666658
Produced by FastQC (version 0.11.5)