##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780580_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 16370 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.940195479535735 34.0 33.0 34.0 27.0 34.0 2 32.78442272449603 34.0 34.0 34.0 31.0 34.0 3 33.15241295051924 34.0 34.0 34.0 32.0 34.0 4 33.278619425778864 34.0 34.0 34.0 33.0 34.0 5 33.31405009163103 34.0 34.0 34.0 33.0 34.0 6 36.93549175320709 38.0 37.0 38.0 35.0 38.0 7 36.490348197923026 38.0 37.0 38.0 34.0 38.0 8 36.39181429444105 38.0 37.0 38.0 34.0 38.0 9 36.2560781918143 38.0 37.0 38.0 34.0 38.0 10-11 35.86282834453268 38.0 36.5 38.0 32.5 38.0 12-13 35.76117898594991 38.0 36.0 38.0 31.0 38.0 14-15 35.97312156383629 38.0 36.5 38.0 32.5 38.0 16-17 36.190317654245575 38.0 37.0 38.0 33.0 38.0 18-19 36.11343921808186 38.0 37.0 38.0 33.0 38.0 20-21 35.834514355528405 38.0 36.0 38.0 31.5 38.0 22-23 35.83430054978619 38.0 36.5 38.0 32.5 38.0 24-25 35.00757483200977 37.0 35.0 38.0 28.5 38.0 26-27 35.23521686010996 37.0 36.0 38.0 30.5 38.0 28-29 35.038362858888206 37.5 35.5 38.0 29.0 38.0 30-31 34.58848503359805 37.0 34.0 38.0 26.5 38.0 32-33 35.19361637141112 37.0 35.5 38.0 29.0 38.0 34-35 35.02987171655467 37.0 35.5 38.0 29.0 38.0 36-37 35.148533903481976 38.0 36.0 38.0 28.5 38.0 38-39 34.952687843616374 37.5 35.0 38.0 28.5 38.0 40-41 34.320739156994506 37.0 34.0 38.0 25.5 38.0 42-43 34.91912034208919 37.0 35.0 38.0 29.0 38.0 44-45 35.162828344532684 37.0 35.5 38.0 29.0 38.0 46-47 35.468204031765424 38.0 36.0 38.0 31.0 38.0 48-49 35.18540012217471 37.5 35.5 38.0 29.0 38.0 50-51 35.498320097739764 37.5 36.0 38.0 31.0 38.0 52-53 35.2595601710446 37.5 36.0 38.0 30.5 38.0 54-55 35.41679902260232 38.0 36.0 38.0 31.0 38.0 56-57 35.530085522296886 38.0 36.0 38.0 31.0 38.0 58-59 35.62180818570556 38.0 36.0 38.0 31.0 38.0 60-61 35.211301160659744 38.0 35.5 38.0 29.0 38.0 62-63 35.513225412339644 38.0 36.0 38.0 31.0 38.0 64-65 35.57464874770922 38.0 36.0 38.0 31.0 38.0 66-67 34.74529627367135 37.0 34.5 38.0 27.0 38.0 68-69 33.81624923640806 37.0 34.0 38.0 23.5 38.0 70-71 33.55803298717166 37.0 34.0 38.0 22.5 38.0 72-73 33.09932803909591 37.0 33.5 38.0 21.5 38.0 74-75 33.82923029932804 37.0 34.0 38.0 23.5 38.0 76 33.42864996945632 37.0 34.0 38.0 22.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8630471655123273 1101 2 0.18893590729116738 1101 3 -0.4931440073882314 1101 4 0.5698188484879907 1101 5 0.7213386079265192 1101 6 -0.8690669521875236 1101 7 1.094785521816462 1101 8 1.2403115878974873 1101 9 -0.4833628560668046 1101 10-11 1.042382691787985 1101 12-13 1.4589902185273687 1101 14-15 1.1587972759224883 1101 16-17 -0.22776329731416212 1101 18-19 -0.3577894233834513 1101 20-21 -0.2096452814017411 1101 22-23 0.6946097298157738 1101 24-25 -1.0702120183975552 1101 26-27 -0.6018592689066367 1101 28-29 1.4363553638392403 1101 30-31 0.5956020361902361 1101 32-33 -0.6178926565275802 1101 34-35 0.3164539309560084 1101 36-37 0.9564481849565851 1101 38-39 0.33268823697770245 1101 40-41 2.5740102643215295 1101 42-43 0.6213037794815719 1101 44-45 0.6200761759591984 1101 46-47 1.030060237433922 1101 48-49 -1.5089068463000928 1101 50-51 -2.354623721241701 1101 52-53 -1.7312867498267508 1101 54-55 -1.0930336761876944 1101 56-57 -1.6159513160055923 1101 58-59 -0.6607587649613933 1101 60-61 -1.902087987529633 1101 62-63 -0.779222968545163 1101 64-65 -1.1159928485472719 1101 66-67 -2.2939354969798984 1101 68-69 -1.2975008250418867 1101 70-71 -0.40105697587758726 1101 72-73 -2.7626520739757012 1101 74-75 -1.031296496628471 1101 76 -1.2162899310812918 1102 1 -0.6695615301398448 1102 2 0.537598114649029 1102 3 0.05200649428400794 1102 4 0.4820262063475198 1102 5 0.15695733702351333 1102 6 0.20283940232752684 1102 7 -0.05320778922032332 1102 8 0.4827865042854427 1102 9 1.1621220937659729 1102 10-11 0.31244958142009693 1102 12-13 1.3390069409353913 1102 14-15 -0.08367764046547421 1102 16-17 0.49506278964235406 1102 18-19 -0.19265564411923464 1102 20-21 0.31292996943438567 1102 22-23 0.49185053249135535 1102 24-25 -4.270044794317286 1102 26-27 -0.7164077638899116 1102 28-29 -1.0113871110771484 1102 30-31 1.8355685913741908 1102 32-33 0.19398025317141787 1102 34-35 -0.1291982429570382 1102 36-37 -3.788953152836058 1102 38-39 0.02541398948606144 1102 40-41 1.2550303312111595 1102 42-43 0.840785384832742 1102 44-45 1.670661460240133 1102 46-47 0.9426856554941239 1102 48-49 -3.942017214193065 1102 50-51 -0.5105601760911895 1102 52-53 0.15667311639398918 1102 54-55 1.0018659893641413 1102 56-57 1.0592159080746768 1102 58-59 -0.06042431680086224 1102 60-61 -6.241134810272108 1102 62-63 -2.5635039050000117 1102 64-65 0.471799793593199 1102 66-67 1.1713655063645803 1102 68-69 -0.07592891868736729 1102 70-71 -1.9566589825665481 1102 72-73 -7.199106923474027 1102 74-75 -2.698938637096699 1102 76 -1.2371929411147349 1103 1 1.6378155190404797 1103 2 1.0795274967423438 1103 3 0.7014390289624473 1103 4 0.6941951849099439 1103 5 0.6333129486250257 1103 6 0.792372819729799 1103 7 1.4023407605905192 1103 8 0.822635180199697 1103 9 -0.05855886461990423 1103 10-11 0.09334491559411617 1103 12-13 -1.0549085697834002 1103 14-15 -0.18991975900267022 1103 16-17 0.7880955765276028 1103 18-19 0.9162661717697915 1103 20-21 0.5310888597244201 1103 22-23 0.6887294732227573 1103 24-25 -4.155921212980587 1103 26-27 -1.0304052418218177 1103 28-29 0.7526418927059595 1103 30-31 1.0209405964183205 1103 32-33 1.2270823969293048 1103 34-35 0.9896542160593569 1103 36-37 -0.27555466607691415 1103 38-39 -0.09674868390612801 1103 40-41 0.9679874560535282 1103 42-43 0.12593670891848774 1103 44-45 1.6418153063939798 1103 46-47 1.7782783414966374 1103 48-49 -1.005699433612989 1103 50-51 0.5384625225216695 1103 52-53 1.0422342765453152 1103 54-55 -0.7473458643558999 1103 56-57 -0.33280805157222915 1103 58-59 -0.8895542033077959 1103 60-61 -4.985302527800478 1103 62-63 -0.9752315216456608 1103 64-65 1.2842209789652017 1103 66-67 0.6853945101476882 1103 68-69 0.11322618850051924 1103 70-71 -0.31463502291963863 1103 72-73 -3.5247689663491855 1103 74-75 -1.3598781074624 1103 76 -4.170673395086997 1104 1 0.5637718031934895 1104 2 0.627341704392613 1104 3 0.4314936737711861 1104 4 -0.49105071672939715 1104 5 -0.5866324065662312 1104 6 0.15412145360958363 1104 7 0.7621768261642927 1104 8 0.9917608632598061 1104 9 0.9941733758172546 1104 10-11 -1.0003709314004183 1104 12-13 0.03580181273026284 1104 14-15 0.8490146672268324 1104 16-17 0.13672945630901978 1104 18-19 0.7233699969064062 1104 20-21 -0.3139931074886917 1104 22-23 0.7623633530041758 1104 24-25 1.1395705902980993 1104 26-27 1.4803871079049529 1104 28-29 1.4142539145638793 1104 30-31 0.8919788477844435 1104 32-33 -0.02332743913627411 1104 34-35 0.4708017570429561 1104 36-37 1.2879699240870224 1104 38-39 1.3613114253834908 1104 40-41 2.2178508440316733 1104 42-43 0.05681102585838005 1104 44-45 0.7834819730606455 1104 46-47 1.0561471939556597 1104 48-49 0.9791366319607846 1104 50-51 0.8439270033959829 1104 52-53 0.8643654240863015 1104 54-55 0.012763425261582029 1104 56-57 -0.5061687073099392 1104 58-59 -1.0911935475700858 1104 60-61 0.25117288203558275 1104 62-63 0.7236755821794745 1104 64-65 0.9782100500034474 1104 66-67 0.7970065320056108 1104 68-69 0.9227890300305788 1104 70-71 1.508725632818063 1104 72-73 0.7547392303721239 1104 74-75 -0.09252838068643854 1104 76 -0.5307826847044836 1105 1 1.983664276311771 1105 2 0.791320199016269 1105 3 0.8820313081797906 1105 4 0.5831428316576961 1105 5 0.10422780848752922 1105 6 -0.0899645678957981 1105 7 0.9734671487449376 1105 8 -1.2442606421165436 1105 9 1.0678292897957462 1105 10-11 0.9098441223630189 1105 12-13 0.1153717052033798 1105 14-15 0.37724047367844804 1105 16-17 1.2571595638359057 1105 18-19 0.990036663573072 1105 20-21 0.7198778602532414 1105 22-23 1.093277331498797 1105 24-25 1.211344783846485 1105 26-27 1.233344097152262 1105 28-29 1.555920581230545 1105 30-31 1.9280003531607903 1105 32-33 1.4532854640895323 1105 34-35 -0.23403695263446167 1105 36-37 0.6952279886031505 1105 38-39 0.1051286296845717 1105 40-41 0.6536035322037108 1105 42-43 0.20089704736375325 1105 44-45 2.1256862741359157 1105 46-47 1.4448568713750163 1105 48-49 0.9767172771220771 1105 50-51 1.3638194765142657 1105 52-53 0.7950105853766161 1105 54-55 1.7106128876271711 1105 56-57 0.6091538733352309 1105 58-59 0.34590322662346296 1105 60-61 1.705205140100098 1105 62-63 0.9855035391687181 1105 64-65 1.0964896198959266 1105 66-67 1.6338344889948502 1105 68-69 1.7088105354069256 1105 70-71 2.2111449876567733 1105 72-73 1.6240940690818064 1105 74-75 0.9781705440447368 1105 76 1.2907226916396013 1106 1 -0.8454874560657721 1106 2 0.4884528155037273 1106 3 0.6389010811785951 1106 4 0.5682085425298595 1106 5 0.6263305563967307 1106 6 0.791158490646616 1106 7 0.5158805298679923 1106 8 0.656575678074617 1106 9 -1.203974772330895 1106 10-11 1.1144438834144026 1106 12-13 2.169135146063603 1106 14-15 -0.2759853327731676 1106 16-17 0.7126553822349493 1106 18-19 1.0548514783878886 1106 20-21 0.9397105962150079 1106 22-23 1.168844834485654 1106 24-25 -0.5983923726648612 1106 26-27 1.8220537745716214 1106 28-29 1.3336983590083165 1106 30-31 1.5179047737103701 1106 32-33 1.316487375678534 1106 34-35 1.8245054607466642 1106 36-37 2.014821775938877 1106 38-39 0.6048299439020113 1106 40-41 1.3002582514390824 1106 42-43 1.74569991474727 1106 44-45 1.7621856767643465 1106 46-47 1.893184230992695 1106 48-49 2.015247743071896 1106 50-51 0.8865195959885739 1106 52-53 1.806958016678891 1106 54-55 0.13683749933565537 1106 56-57 0.3558683297271017 1106 58-59 1.1439916376151018 1106 60-61 1.316913622776326 1106 62-63 1.8866385451424321 1106 64-65 -1.1051232833298812 1106 66-67 0.4701546801537546 1106 68-69 2.2987149559565054 1106 70-71 1.4226145217069543 1106 72-73 1.6399244155573172 1106 74-75 0.10839754523948386 1106 76 1.8618099078881087 1107 1 0.09710513652682096 1107 2 -0.12265829560738695 1107 3 0.5981603404378575 1107 4 0.62561594993727 1107 5 0.28003426010043597 1107 6 0.35412145360957936 1107 7 1.178843492830957 1107 8 -0.4582391367401968 1107 9 -0.5891599575160811 1107 10-11 0.8829624019329216 1107 12-13 -0.53919818726974 1107 14-15 0.41568133389349526 1107 16-17 0.45339612297568266 1107 18-19 0.7067033302397405 1107 20-21 0.44434022584463406 1107 22-23 0.12903001967084293 1107 24-25 0.05623725696476356 1107 26-27 -0.2362795587617157 1107 28-29 1.4892539145638821 1107 30-31 1.7503121811177778 1107 32-33 1.710005894197053 1107 34-35 0.9708017570429632 1107 36-37 1.5129699240870167 1107 38-39 1.161311425383495 1107 40-41 1.5178508440316776 1107 42-43 1.2068110258583857 1107 44-45 1.500148639727314 1107 46-47 1.722813860622324 1107 48-49 1.4208032986274475 1107 50-51 1.2605936700626472 1107 52-53 1.1143654240863015 1107 54-55 1.0544300919282463 1107 56-57 1.352164626023395 1107 58-59 1.100473119096577 1107 60-61 1.6511728820355884 1107 62-63 0.6320089155128059 1107 64-65 0.24487671667011313 1107 66-67 0.43033986533893653 1107 68-69 0.4394556966972516 1107 70-71 0.4087256328180686 1107 72-73 1.6630725637054553 1107 74-75 -0.1425283806864428 1107 76 1.7025506486288506 1108 1 0.40186704128872464 1108 2 0.9785321805830947 1108 3 0.8267317690092852 1108 4 0.3982349975563153 1108 5 -0.27710859704242097 1108 6 -0.25183092734280166 1108 7 0.4014625404500052 1108 8 -1.2177629462640098 1108 9 0.36679242343630136 1108 10-11 1.5287957352662502 1108 12-13 1.4399684793969314 1108 14-15 0.8758003815125477 1108 16-17 0.5575627896423541 1108 18-19 1.0614652350016414 1108 20-21 1.7538640353684443 1108 22-23 1.1367681149089393 1108 24-25 1.87349916172667 1108 26-27 1.0274109174287602 1108 28-29 0.118420581230545 1108 30-31 1.0622169430225412 1108 32-33 0.7070297037208633 1108 34-35 1.3797303284715312 1108 36-37 2.2879699240870224 1108 38-39 1.6607161872882585 1108 40-41 1.5922556059364368 1108 42-43 -0.4408080217606596 1108 44-45 1.1596724492511186 1108 46-47 1.5436471939556569 1108 48-49 2.0291366319607818 1108 50-51 1.7641650986340807 1108 52-53 0.759008281229157 1108 54-55 0.8782396157377761 1108 56-57 0.9349027212614942 1108 58-59 0.6105921667156267 1108 60-61 1.522601453464155 1108 62-63 1.6081993917032804 1108 64-65 1.6002338595272576 1108 66-67 2.470816055815135 1108 68-69 2.4233842681258153 1108 70-71 0.24801134710378392 1108 72-73 -0.17918934105644269 1108 74-75 -0.6062188568769145 1108 76 0.9358839819621849 1109 1 -3.701379711958026 1109 2 0.15612958318049408 1109 3 -0.06547602319850654 1109 4 -0.354687080365764 1109 5 -0.042693012626841664 1109 6 -0.18527248578435973 1109 7 -1.878732264744798 1109 8 0.6932760147749519 1109 9 -0.5861296544857808 1109 10-11 -0.46552244655193675 1109 12-13 0.0024684793969314 1109 14-15 0.6467419399541043 1109 16-17 1.0882446078241728 1109 18-19 0.17185484539125184 1109 20-21 1.0905523470567644 1109 22-23 0.46160577724659646 1109 24-25 -0.4127021369746302 1109 26-27 -1.4688553163374678 1109 28-29 -3.753170327860367 1109 30-31 0.45864551445111346 1109 32-33 -0.38469107549991577 1109 34-35 -0.5246527884115793 1109 36-37 1.8766062877233907 1109 38-39 1.0923720314441 1109 40-41 -0.13290673172590317 1109 42-43 0.47347769252505145 1109 44-45 0.3054516700303438 1109 46-47 0.6970562848647504 1109 48-49 0.7223184501425948 1109 50-51 -0.280315420846442 1109 52-53 -0.9219982122773445 1109 54-55 -0.881175968677816 1109 56-57 -0.07662325276447746 1109 58-59 -1.3457390021155433 1109 60-61 0.9898092456719496 1109 62-63 0.03579679430068694 1109 64-65 0.9085130803064843 1109 66-67 -0.19314498314590622 1109 68-69 -0.033271576030031724 1109 70-71 -0.5344561853637515 1109 72-73 0.21383013946304175 1109 74-75 -1.2826798958379584 1109 76 -1.073206927128723 1110 1 -1.1536040833313344 1110 2 -0.5552824090825652 1110 3 0.17475608511870888 1110 4 0.48980034710038467 1110 5 0.5321619196748983 1110 6 0.5768164890705734 1110 7 0.8972832091430121 1110 8 1.44105164340165 1110 9 0.7775067091505861 1110 10-11 -0.08157660515927745 1110 12-13 0.31097911769480646 1110 14-15 0.6335891353119365 1110 16-17 0.71048832155725 1110 18-19 -0.7120909960014004 1110 20-21 1.065794126553861 1110 22-23 -0.67611182430079 1110 24-25 -1.3003230267231771 1110 26-27 1.5986495192524686 1110 28-29 0.17294185782629512 1110 30-31 -0.7200778898042088 1110 32-33 0.272949156608405 1110 34-35 0.8442060123621147 1110 36-37 1.0831826900444668 1110 38-39 0.32390007786576547 1110 40-41 -1.917432843911591 1110 42-43 -0.4712031585387848 1110 44-45 0.9396876468195074 1110 46-47 0.4997642152322541 1110 48-49 1.6594557808969483 1110 50-51 -0.8425978192990584 1110 52-53 -0.009038831232857092 1110 54-55 -0.9709245180008352 1110 56-57 0.8454270373709178 1110 58-59 -0.6422573773573248 1110 60-61 0.1985133075675023 1110 62-63 -0.29263647455811537 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-2.00716045129775 2116 62-63 -0.4466947881908965 2116 64-65 -3.318086246292843 2116 66-67 -2.252067542068467 2116 68-69 -2.367951710710159 2116 70-71 1.5800219291143591 2116 72-73 -0.684149658516759 2116 74-75 -0.9934543066123709 2116 76 -3.7863382402600365 2117 1 1.515221078555804 2117 2 1.2106750377259488 2117 3 0.8981603404378546 2117 4 -0.6693115862946186 2117 5 -0.25692226163869947 2117 6 0.3048460912907416 2117 7 1.2904376957295085 2117 8 0.5446594139844407 2117 9 -0.005101986501585998 2117 10-11 -0.9576173082120079 2117 12-13 -0.6931836945161152 2117 14-15 1.1479277107050976 2117 16-17 -0.042980688618520446 2117 18-19 -1.444745945122584 2117 20-21 -0.11181919444521071 2117 22-23 1.1837401645983832 2117 24-25 -0.2984728879627738 2117 26-27 -0.7431636167327227 2117 28-29 -1.2919055057259783 2117 30-31 0.562993340538064 2117 32-33 -1.4222404826145407 2117 34-35 0.9468887135646966 2117 36-37 0.782535141478327 2117 38-39 1.528340410890742 2117 40-41 -0.3499027791567286 2117 42-43 -0.5743483944314747 2117 44-45 -0.5320977370842854 2117 46-47 -2.4155919364791245 2117 48-49 -0.4871677158653043 2117 50-51 -1.0285367647199593 2117 52-53 0.8882784675645539 2117 54-55 0.25479241076882886 2117 56-57 1.416657379646587 2117 58-59 -0.921628330178784 2117 60-61 0.19573809942689024 2117 62-63 0.4164292053678764 2117 64-65 -0.7029493702864045 2117 66-67 0.053890589976617775 2117 68-69 1.3003252619146366 2117 70-71 1.1532908502093733 2117 72-73 2.4003914042851733 2117 74-75 3.0991382859802243 2117 76 2.6967535471795756 2118 1 -0.374106984685298 2118 2 0.11976594681685526 2118 3 -1.3745669322894187 2118 4 0.3089492832706 2118 5 0.8300342601004331 2118 6 0.6783638778520071 2118 7 1.6758131898006567 2118 8 1.4023669238658627 2118 9 -1.3134023817585074 2118 10-11 -0.738249719279203 2118 12-13 -0.4748042478757952 2118 14-15 0.6103783035904726 2118 16-17 -1.4026644830849122 2118 18-19 -0.8735997000632878 2118 20-21 0.25873416523857173 2118 22-23 1.3388785045193288 2118 24-25 0.7736614993890072 2118 26-27 -2.9961280436101987 2118 28-29 -0.08044305513309524 2118 30-31 -0.3095363037307024 2118 32-33 -0.20287289368172878 2118 34-35 -0.742834606593398 2118 36-37 -2.723393712276618 2118 38-39 -1.4439916049195318 2118 40-41 0.4761841773650062 2118 42-43 2.5689322379795954 2118 44-45 -3.0400028754242 2118 46-47 0.424329012137477 2118 48-49 -0.9367724589483117 2118 50-51 -0.5848608753918967 2118 52-53 0.600729060449936 2118 54-55 -0.5129941504959916 2118 56-57 0.6824676563264234 2118 58-59 1.0724428160662711 2118 60-61 -0.08291802705532092 2118 62-63 -0.6142032056993116 2118 64-65 0.5994221712155721 2118 66-67 -0.011326801327726344 2118 68-69 -3.005998848757301 2118 70-71 -2.079910730818291 2118 72-73 -0.272533496900607 2118 74-75 -0.1281344412925023 2118 76 0.9813385274167317 2119 1 1.9627914110366262 2119 2 -0.14226613874463823 2119 3 0.7805132816143256 2119 4 -0.779286010847045 2119 5 0.7123872012769041 2119 6 -0.3243099189394414 2119 7 -2.481940820894536 2119 8 -1.854317568112748 2119 9 -2.869552114378827 2119 10-11 -1.9013513235572859 2119 12-13 -3.0563550500148366 2119 14-15 0.1210734907562454 2119 16-17 -1.5417019162399939 2119 18-19 -2.127610395250457 2119 20-21 -2.6757578133710425 2119 22-23 -1.3469503724860203 2119 24-25 0.9969235314745717 2119 26-27 -0.9426521077813206 2119 28-29 -1.532314712887107 2119 30-31 1.7292337497452266 2119 32-33 0.7810843255696085 2119 34-35 -1.7027276547217483 2119 36-37 -4.939971252383565 2119 38-39 -4.403884653047879 2119 40-41 -4.435580528517349 2119 42-43 0.7146541631132806 2119 44-45 1.2300506005116247 2119 46-47 0.5179118998380119 2119 48-49 -0.24292219156863126 2119 50-51 -0.6717592711138209 2119 52-53 -0.2562228112078202 2119 54-55 0.057861464477262814 2119 56-57 -2.4311687073099364 2119 58-59 0.17939468772402734 2119 60-61 -0.6444153532585304 2119 62-63 -1.8214224570362063 2119 64-65 -0.44335857744752616 2119 66-67 -1.4525032719159583 2119 68-69 0.18651452022665893 2119 70-71 -0.06921554365251836 2119 72-73 -0.6187901813925833 2119 74-75 -2.6655675963727177 2119 76 0.9064722172563009 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 2.0 20 0.0 21 0.0 22 2.0 23 5.0 24 20.0 25 20.0 26 63.0 27 109.0 28 195.0 29 285.0 30 448.0 31 697.0 32 991.0 33 1456.0 34 2090.0 35 2762.0 36 3731.0 37 3494.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.22113622480147 26.994502138057424 11.172877214416616 27.611484422724498 2 17.04337202199145 19.511301160659745 40.287110568112396 23.158216249236407 3 16.30421502748931 27.574832009773974 30.91020158827123 25.210751374465485 4 11.576053756872327 20.757483200977397 37.63591936469151 30.030543677458766 5 11.533292608430056 39.8167379352474 36.224801466096515 12.425167990226022 6 25.55894929749542 39.49297495418448 17.75198533903482 17.19609040928528 7 24.288332315210752 32.877214416615764 23.029932803909592 19.8045204642639 8 21.172877214416616 36.065974343310934 22.87721441661576 19.88393402565669 9 21.63714111178986 15.50397067806964 22.339645693341478 40.51924251679902 10-11 23.081857055589495 25.595601710445937 28.9034819792303 22.419059254734268 12-13 23.83017715332926 21.499694563225415 27.7550397067807 26.91508857666463 14-15 20.919364691508857 25.62919975565058 23.204031765424556 30.247403787416005 16-17 21.514966401954798 28.49419670128284 24.813683567501528 25.177153329260843 18-19 21.89981673793525 27.60537568723274 25.21686010995724 25.27794746487477 20-21 21.75320708613317 26.71960904092853 25.10079413561393 26.426389737324374 22-23 22.541233964569336 28.283445326817347 26.334758704948076 22.84056200366524 24-25 22.47403787416005 29.798411728772145 24.862553451435552 22.864996945632253 26-27 21.661576053756875 29.49602932193036 27.037263286499698 21.805131337813073 28-29 21.927306047648138 27.483200977397676 28.91875381795968 21.670739156994504 30-31 20.717776420281 26.728772144166157 27.86805131337813 24.685400122174713 32-33 25.16493585827734 26.160659743433108 25.39401343921808 23.28039095907147 34-35 22.125839951130118 27.492364080635305 27.88637751985339 22.495418448381184 36-37 22.54428833231521 27.07391569945021 27.101405009163106 23.28039095907147 38-39 22.06169822846671 26.68295662797801 27.07391569945021 24.18142944410507 40-41 20.678069639584606 26.45693341478314 29.566279780085523 23.29871716554673 42-43 22.498472816127062 26.957849725106904 29.410507025045817 21.13317043372022 44-45 19.575442883323152 26.847892486255343 28.36285888821014 25.213805742211363 46-47 22.700061087354918 25.583384239462433 28.21014050091631 23.50641417226634 48-49 20.64752596212584 25.711667684789248 28.359804520464266 25.281001832620646 50-51 19.554062309102015 25.56505803298717 29.664019547953576 25.21686010995724 52-53 20.525351252290776 25.861331704337204 31.66463042150275 21.948686621869275 54-55 19.65791081246182 25.461209529627364 30.314599877825287 24.566279780085523 56-57 22.092241905925473 25.586438607208308 28.708002443494195 23.613317043372025 58-59 19.23640806353085 29.664019547953576 28.439218081857053 22.660354306658522 60-61 20.09773976786805 31.062919975565055 26.792913866829565 22.046426389737324 62-63 17.953573610262676 33.002443494196704 26.044593769089797 22.999389126450826 64-65 19.285277947464873 33.37507635919364 24.773976786805132 22.565668906536345 66-67 18.8637751985339 32.91386682956628 25.26878436163714 22.953573610262676 68-69 18.918753817959683 31.94257788637752 25.784972510690285 23.353695784972512 70-71 19.51435552840562 31.43249847281613 25.271838729383017 23.781307269395235 72-73 19.089798411728772 30.189370800244347 25.430665852168598 25.29016493585828 74-75 20.299328039095908 29.752596212583992 24.78313989004276 25.16493585827734 76 19.48686621869273 29.35247403787416 25.387904703726328 25.77275503970678 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 1.0 18 3.0 19 4.5 20 5.5 21 7.0 22 8.0 23 8.5 24 17.5 25 27.0 26 38.5 27 57.5 28 74.0 29 83.0 30 124.5 31 181.5 32 235.0 33 273.0 34 326.5 35 414.0 36 480.0 37 512.0 38 536.5 39 690.0 40 899.0 41 1073.0 42 1167.0 43 1256.0 44 1382.5 45 1400.0 46 1380.0 47 1340.5 48 1243.5 49 1076.5 50 967.0 51 875.5 52 677.0 53 506.5 54 443.0 55 415.0 56 331.5 57 255.0 58 234.0 59 183.5 60 103.5 61 64.5 62 55.0 63 38.0 64 20.5 65 14.5 66 6.0 67 3.0 68 2.0 69 0.5 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 16370.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.44349419670129 #Duplication Level Percentage of deduplicated Percentage of total 1 73.28311485032283 45.76053756872327 2 14.34161612208961 17.910812461820402 3 6.172960281745255 11.56383628588882 4 2.905497945607513 7.25717776420281 5 1.5065544903150068 4.703726328649969 6 0.714145959694776 2.6756261453879047 7 0.36196439053022894 1.5821624923640805 8 0.215222070045001 1.0751374465485644 9 0.1271766777538642 0.7147220525351252 >10 0.33261592643318333 2.7306047648136835 >50 0.019565642731363724 0.7208307880268785 >100 0.019565642731363724 3.304825901038485 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT 368 2.24801466096518 RNA PCR Primer, Index 30 (95% over 22bp) CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT 173 1.056811240073305 No Hit CTTTACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC 63 0.38485033598045204 No Hit ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 55 0.3359804520464264 No Hit TATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCT 32 0.19547953573610263 RNA PCR Primer, Index 30 (95% over 21bp) ATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCGTCTT 25 0.1527183872938302 No Hit CTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC 19 0.11606597434331094 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.006108735491753207 0.0 2 0.0 0.0 0.0 0.006108735491753207 0.0 3 0.0 0.0 0.0 0.006108735491753207 0.0 4 0.0 0.0 0.0 0.006108735491753207 0.0 5 0.0 0.0 0.0 0.006108735491753207 0.0 6 0.0 0.0 0.0 0.006108735491753207 0.0 7 0.0 0.0 0.0 0.006108735491753207 0.0 8 0.0 0.0 0.0 0.006108735491753207 0.0 9 0.0 0.0 0.0 0.006108735491753207 0.0 10 0.0 0.0 0.0 0.006108735491753207 0.0 11 0.0 0.0 0.0 0.01832620647525962 0.0 12 0.0 0.0 0.0 0.01832620647525962 0.0 13 0.0 0.0 0.0 0.01832620647525962 0.0 14 0.0 0.0 0.0 0.01832620647525962 0.0 15 0.0 0.0 0.0 0.01832620647525962 0.0 16 0.0 0.0 0.0 0.02443494196701283 0.0 17 0.0 0.0 0.0 0.030543677458766034 0.0 18 0.0 0.0 0.0 0.030543677458766034 0.0 19 0.0 0.0 0.0 0.03665241295051924 0.0 20 0.0 0.0 0.0 0.03665241295051924 0.0 21 0.0 0.0 0.0 0.05497861942577886 0.0 22 0.0 0.0 0.0 0.05497861942577886 0.0 23 0.0 0.0 0.0 0.06108735491753207 0.0 24 0.0 0.0 0.0 0.06719609040928527 0.0 25 0.0 0.0 0.0 0.07330482590103848 0.0 26 0.0 0.0 0.0 0.07330482590103848 0.0 27 0.0 0.0 0.0 0.0855222968845449 0.0 28 0.0 0.0 0.0 0.0855222968845449 0.0 29 0.0 0.0 0.0 0.11606597434331094 0.0 30 0.0 0.0 0.0 0.12217470983506414 0.0 31 0.0 0.0 0.0 0.14050091631032377 0.0 32 0.0 0.0 0.0 0.15271838729383017 0.0 33 0.0 0.0 0.0 0.1893708002443494 0.0 34 0.0 0.0 0.0 0.21991447770311545 0.0 35 0.0 0.0 0.0 0.30543677458766033 0.0 36 0.0 0.0 0.0 0.38485033598045204 0.0 37 0.0 0.0 0.0 0.531459987782529 0.0 38 0.0 0.0 0.0 0.6414172266340867 0.0 39 0.0 0.0 0.0 0.8124618204031765 0.0 40 0.0 0.0 0.0 1.0079413561392792 0.0 41 0.0 0.0 0.0 1.2461820403176542 0.0 42 0.0 0.0 0.0 1.502748930971289 0.0 43 0.0 0.0 0.0 1.77153329260843 0.0 44 0.0 0.0 0.0 2.0464263897373245 0.0 45 0.0 0.0 0.0 2.370189370800244 0.0 46 0.0 0.0 0.0 2.7672571777642028 0.0 47 0.0 0.0 0.0 3.0849114233353694 0.0 48 0.0 0.0 0.0 3.39034819792303 0.0 49 0.0 0.0 0.0 3.7507635919364692 0.0 50 0.0 0.0 0.0 4.160048869883934 0.0 51 0.0 0.0 0.0 4.37996334758705 0.0 52 0.0 0.0 0.0 4.673182651191204 0.0 53 0.0 0.0 0.0 5.051924251679902 0.0 54 0.0 0.0 0.0 5.387904703726329 0.0 55 0.0 0.0 0.0 5.760537568723274 0.0 56 0.0 0.0 0.0 6.059865607819181 0.0 57 0.0 0.0 0.0 6.377519853390348 0.0 58 0.0 0.0 0.0 6.701282834453268 0.0 59 0.0 0.0 0.0 7.000610873549175 0.0 60 0.0 0.0 0.0 7.27550397067807 0.0 61 0.0 0.0 0.0 7.4893097128894315 0.0 62 0.0 0.0 0.0 7.739767868051313 0.0 63 0.0 0.0 0.0 7.910812461820403 0.0 64 0.0 0.0 0.0 8.130726939523518 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTCCTT 15 0.0021857778 70.0 34 ATGTCCT 15 0.0021857778 70.0 33 CCACCAT 20 0.0068307077 52.5 20 GGATCTC 115 1.2478267E-9 30.434784 32 GCCGGAT 130 1.382432E-10 29.615383 29 ATCTCGT 120 1.9663275E-9 29.166666 34 CGGATCT 120 1.9663275E-9 29.166666 31 TCTCGTA 120 1.9663275E-9 29.166666 35 GATCTCG 120 1.9663275E-9 29.166666 33 CCGGATC 130 4.62569E-9 26.923077 30 TCTGCTT 145 4.9294613E-10 26.551725 50 GTATGCC 150 7.3123374E-10 25.666666 42 TGCCGGA 150 7.3123374E-10 25.666666 28 TATGCCG 150 7.3123374E-10 25.666666 43 ACATTGC 150 7.3123374E-10 25.666666 24 ATTGCCG 150 7.3123374E-10 25.666666 26 CGTATGC 150 7.3123374E-10 25.666666 38 CTGCTTG 150 7.3123374E-10 25.666666 51 TCGTATG 150 7.3123374E-10 25.666666 37 TTGAAAA 150 7.3123374E-10 25.666666 58 >>END_MODULE