FastQCFastQC Report
Wed 25 May 2016
SRR1780579_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780579_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200941
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCGTCCTTTAGTGTTGTGTATGGTTATTATTTGTTTTGAGGTTAGTTT2900.1443209698369173No Hit
ATAATAAAGGTGGATGCGACAATGGATTTTACATAATGGGGGTATGAGTT2190.10898721515270651No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACGA207.8230293E-444.0204312
AAATCGT207.851885E-443.9875532
CGTAGCC250.002345658635.20757736
CGCTACG250.002345658635.20757711
CAATAGT250.002345658635.20757736
AATCGTA250.002351400235.1900433
CAGCGCT250.002354275435.1812829
ACATTGG300.00571378529.35427321
AAATTGC300.00571378529.35427318
CTAGCTG300.00571378529.35427320
ACGAAAC300.00571378529.35427324
GTCTACT300.00572075229.3469581
ACAGGGG300.00572075229.3469581
GCCGTAC300.00572075229.3469581
CCGCACG300.00572075229.34695814
TAACCGC300.00572772529.33964511
ATGGAAA300.00573470529.33233830
TCCATAC300.005741691729.32503527
AAAATCG300.005741691729.32503531
ACTTGAT454.0676037E-529.31773443
Produced by FastQC (version 0.11.5)