FastQCFastQC Report
Wed 25 May 2016
SRR1780579_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780579_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200941
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT92594.607820205931095No Hit
ATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTCTT43482.1638192305204016TruSeq Adapter, Index 2 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC13390.6663647538332147No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCG9370.4663060301282466No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC5970.29710213445737804No Hit
CTTTACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTC5010.24932691685619163No Hit
TATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTCT3680.18313833413788125No Hit
CTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTC2460.1224239951030402No Hit
CTACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTACC2310.11495911735285483No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGATT207.8446727E-443.9957741
GCACCGA207.8446727E-443.9957739
CACCGAT207.8446727E-443.9957740
ATTTACT357.26504E-637.710667
GACATAT357.26504E-637.7106637
CCGTCTT4750.037.04907244
GTCCCCT250.002334935835.2404671
TTACCAG250.002346381335.20537616
GGGCACC250.002349249635.19661337
CGGAGTA250.002349249635.19661332
AGCGGAT950.034.733535
CATGGAT451.0302756E-634.2274483
GCCGTCT5200.033.842943
ATGATTC1000.032.99682640
GAAGGTA1000.032.99682629
GGTAGCG1000.032.99682632
ATTGGCG1100.031.99692521
AAAACGT353.2055174E-431.43337414
AAACGTA353.2055174E-431.43337415
TTGCAGG353.2055174E-431.4333744