FastQCFastQC Report
Wed 25 May 2016
SRR1780578_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780578_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28842
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGGG150.002203318569.9965249
TGGTTTA200.0068851152.49739527
ACATGTC200.0068851152.49739520
CCGCATT200.0068851152.49739547
GTTCACC200.0068851152.49739539
GACATGT200.0068851152.49739519
ATTATCC200.0068851152.49739531
ACTCAAC200.0068851152.49739560
TACCGCA200.0068851152.49739545
CTGTGGT200.0068851152.4973959
ACAAGGA200.0068851152.4973953
CGCATTC200.0068851152.49739548
AGTTCAC200.0068851152.49739538
CTACCGC200.0068851152.49739544
ACTACCG200.0068851152.49739543
TTCACCC305.785341E-446.6643540
GAGGCAA654.655776E-737.69043770
CGAGGCA654.655776E-737.69043769
ACGAGGC654.655776E-737.69043768
TGAAGAG400.002381191834.99826435
Produced by FastQC (version 0.11.5)