##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780578_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 28842 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.800741973510853 34.0 31.0 34.0 12.0 34.0 2 30.941786283891545 34.0 32.0 34.0 21.0 34.0 3 30.950419527078566 34.0 33.0 34.0 21.0 34.0 4 31.305526662506068 34.0 34.0 34.0 23.0 34.0 5 31.202829207405866 34.0 34.0 34.0 21.0 34.0 6 34.19270508286527 38.0 35.0 38.0 23.0 38.0 7 33.190832813258446 37.0 34.0 38.0 21.0 38.0 8 32.34245197975175 37.0 31.0 38.0 11.0 38.0 9 33.32230774564871 37.0 34.0 38.0 21.0 38.0 10-11 33.53349282296651 37.0 34.0 38.0 21.0 38.0 12-13 33.73120796061299 38.0 34.0 38.0 21.0 38.0 14-15 33.52160044379724 38.0 34.0 38.0 21.0 38.0 16-17 33.39484085708342 38.0 34.0 38.0 21.0 38.0 18-19 33.44958740725331 37.5 34.0 38.0 16.5 38.0 20-21 33.48854101657305 38.0 34.0 38.0 21.0 38.0 22-23 33.37098675542612 37.5 34.0 38.0 21.0 38.0 24-25 33.76043616947507 38.0 34.0 38.0 21.0 38.0 26-27 33.734987171486026 38.0 34.0 38.0 21.0 38.0 28-29 33.87310172664864 38.0 35.0 38.0 21.0 38.0 30-31 33.79283683517093 38.0 34.0 38.0 21.0 38.0 32-33 33.418382913806255 38.0 34.0 38.0 16.0 38.0 34-35 33.537618750433396 38.0 34.0 38.0 21.0 38.0 36-37 33.56847652728659 38.0 34.0 38.0 21.0 38.0 38-39 33.91976978018168 38.0 35.0 38.0 21.0 38.0 40-41 33.50419527078566 37.5 34.0 38.0 21.0 38.0 42-43 33.71012759170654 38.0 34.0 38.0 21.0 38.0 44-45 32.87674225088413 37.0 33.0 38.0 16.0 38.0 46-47 33.552648914777066 37.0 34.0 38.0 21.0 38.0 48-49 33.44007003675196 37.0 34.0 38.0 21.0 38.0 50-51 33.25889328063241 37.0 34.0 38.0 21.0 38.0 52-53 33.27470355731225 37.0 34.0 38.0 21.0 38.0 54-55 33.0819464669579 37.0 33.5 38.0 20.0 38.0 56-57 33.05192080992997 37.0 33.5 38.0 15.0 38.0 58-59 33.016815754802025 37.0 33.0 38.0 15.5 38.0 60-61 32.994279176201374 37.0 32.5 38.0 15.0 38.0 62-63 32.98439775327647 37.0 33.0 38.0 15.0 38.0 64-65 33.106147285209076 37.0 33.0 38.0 19.0 38.0 66-67 33.00629290617849 37.0 33.0 38.0 19.0 38.0 68-69 32.48517786561265 37.0 31.5 38.0 15.0 38.0 70-71 32.63724776367797 37.0 32.0 38.0 11.0 38.0 72-73 32.76189237916927 37.0 32.0 38.0 15.0 38.0 74-75 32.30107135427502 37.0 31.0 38.0 11.0 38.0 76 31.70896609111712 36.0 29.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -1.622098834326735 1101 2 -0.3284206561136074 1101 3 0.9410620927706113 1101 4 -1.6032218784649217 1101 5 0.05168630552511999 1101 6 -1.5487026420090757 1101 7 -0.7640070227815059 1101 8 -0.7098165132378043 1101 9 -1.304852211713591 1101 10-11 -0.6684933383005713 1101 12-13 -2.2612798617078305 1101 14-15 -0.22043289322721193 1101 16-17 -1.4120608705216355 1101 18-19 0.20317517402263263 1101 20-21 -0.6846486551725306 1101 22-23 -0.6007204370093575 1101 24-25 -1.0668902952099941 1101 26-27 -0.6793892290397778 1101 28-29 0.6117649569094255 1101 30-31 -1.243738717939891 1101 32-33 0.3385159518455083 1101 34-35 -1.3093896225049093 1101 36-37 -0.025980817505228515 1101 38-39 -0.7270528949450963 1101 40-41 -0.09865104782806355 1101 42-43 -0.8482362915442252 1101 44-45 -0.09400987420451656 1101 46-47 0.2092498709111581 1101 48-49 -1.846092679749603 1101 50-51 -1.9804557509417542 1101 52-53 -0.057537994812591364 1101 54-55 -1.5548801875284823 1101 56-57 -0.22456975436966786 1101 58-59 0.22863262641424598 1101 60-61 -1.2082341935078702 1101 62-63 -1.0816004937840056 1101 64-65 -0.1502093804731075 1101 66-67 -0.5377279678880456 1101 68-69 0.10649685180415247 1101 70-71 -0.3965449571689774 1101 72-73 -0.2744735923722601 1101 74-75 -2.021214333756351 1101 76 -1.5239900831990845 1102 1 -0.5665432787711815 1102 2 -1.0366077906165323 1102 3 -0.47940574348669784 1102 4 -0.12252012407895663 1102 5 0.0013939078643012692 1102 6 -0.07209445487457344 1102 7 0.7751742637682 1102 8 0.38024196629435636 1102 9 0.23549866547939047 1102 10-11 -0.49334713947015274 1102 12-13 -4.220051791532391 1102 14-15 -1.3985030686658106 1102 16-17 -1.8489029757847923 1102 18-19 -1.6988716096031027 1102 20-21 -1.737865029441533 1102 22-23 -1.4931180978280771 1102 24-25 -0.41981427181817565 1102 26-27 -5.892254726115798 1102 28-29 -0.5361882594648506 1102 30-31 -0.28555158343696263 1102 32-33 -7.276396328856244 1102 34-35 -1.2438925464815185 1102 36-37 -0.6268580104876804 1102 38-39 -0.03611722243047666 1102 40-41 0.005150121762589777 1102 42-43 -0.15905500499452074 1102 44-45 -5.052781804029074 1102 46-47 -1.2489372635917704 1102 48-49 -0.3782564224396623 1102 50-51 -6.709695517023626 1102 52-53 -1.8683567082628834 1102 54-55 -0.0788567957156161 1102 56-57 -1.5681370058316517 1102 58-59 -0.2125954437611881 1102 60-61 -0.19244471982365496 1102 62-63 -5.281892891444819 1102 64-65 -1.102548561759658 1102 66-67 -0.7052718275371674 1102 68-69 -7.838239990301108 1102 70-71 -0.8880654250052373 1102 72-73 -0.4595613116705124 1102 74-75 -5.103962871768047 1102 76 -1.382469615362826 1103 1 -0.22342401271613355 1103 2 -0.01149914744897984 1103 3 0.47327412131291524 1103 4 0.34479035395098023 1103 5 0.38236928207965803 1103 6 -1.5151654350773711 1103 7 1.5299787060666077 1103 8 0.9682670749375291 1103 9 0.7618144549530754 1103 10-11 1.0033211367249137 1103 12-13 -1.6238180880075248 1103 14-15 -0.08266530912935366 1103 16-17 0.2072051845242342 1103 18-19 -0.09718160477451931 1103 20-21 -0.4324570139900601 1103 22-23 -0.09022094669335701 1103 24-25 0.6532422226289469 1103 26-27 -0.8486043156860532 1103 28-29 0.16762632286253165 1103 30-31 0.22511958990924086 1103 32-33 -4.330138482405253 1103 34-35 0.08918326230650564 1103 36-37 0.13166444243361042 1103 38-39 0.4355872681537818 1103 40-41 0.3937546606326876 1103 42-43 1.019602648313061 1103 44-45 -4.718015990412464 1103 46-47 0.43029981994276056 1103 48-49 1.0831631816163494 1103 50-51 0.41444740909901867 1103 52-53 0.5717688349529553 1103 54-55 0.44369269728293403 1103 56-57 0.7733405412470518 1103 58-59 0.37465709124605695 1103 60-61 0.32112360465727363 1103 62-63 0.31009165708245945 1103 64-65 0.4920434227888677 1103 66-67 -0.12289616360669697 1103 68-69 -3.0811178270949284 1103 70-71 0.7939239521072707 1103 72-73 2.029043227199601 1103 74-75 -2.3438952715749046 1103 76 0.7612290809191613 1104 1 -2.412087833226625 1104 2 -2.0928871027582723 1104 3 -0.679509964435109 1104 4 -0.38870042631970847 1104 5 -0.034562319337364755 1104 6 -1.7070084725921362 1104 7 0.07053643156392297 1104 8 0.027037172130732756 1104 9 0.42518079158674027 1104 10-11 -1.6030917981465507 1104 12-13 -1.3898277164933077 1104 14-15 -1.3047563255704517 1104 16-17 -2.199189583392922 1104 18-19 -0.4000701505098192 1104 20-21 -0.559126102917304 1104 22-23 -0.8810284678124418 1104 24-25 -0.608089415121988 1104 26-27 -0.2723485249693667 1104 28-29 -1.3379600155878322 1104 30-31 -0.8421435584239347 1104 32-33 -1.6477876785175276 1104 34-35 -0.6867273562782898 1104 36-37 -0.49809302872634476 1104 38-39 -0.32501769142474757 1104 40-41 -0.3165278355068253 1104 42-43 -0.033989866901762866 1104 44-45 -3.187904263643457 1104 46-47 -0.9220482589018246 1104 48-49 0.10319224874324817 1104 50-51 0.571909485581898 1104 52-53 -1.000717312744385 1104 54-55 -1.6143961391236417 1104 56-57 -0.94838713610784 1104 58-59 0.2542101841700273 1104 60-61 -0.4131846885573651 1104 62-63 -0.25349268300292493 1104 64-65 -0.8020795674918091 1104 66-67 -0.7428434794391947 1104 68-69 -4.616770474928515 1104 70-71 -0.7225625589291553 1104 72-73 -0.38916006101912615 1104 74-75 -0.5261098233053048 1104 76 0.48173048885812264 1105 1 -0.31233767129454293 1105 2 0.03471537711588368 1105 3 -0.03474268396874436 1105 4 -0.3166175170942047 1105 5 0.6344485069789094 1105 6 0.2526473060698464 1105 7 1.7720262067096613 1105 8 0.32092076609760767 1105 9 0.08891725869139577 1105 10-11 1.6452657478884234 1105 12-13 -0.5639278367857088 1105 14-15 0.8906262968039371 1105 16-17 0.6299952042447217 1105 18-19 0.2530713318627136 1105 20-21 -0.23220836441448256 1105 22-23 0.22316325977152474 1105 24-25 0.8088625604494055 1105 26-27 0.3387831489456872 1105 28-29 -0.5638321355101041 1105 30-31 0.19172337967174258 1105 32-33 1.2794816839327439 1105 34-35 0.6677651023133677 1105 36-37 1.4433338242393177 1105 38-39 0.8250239482532393 1105 40-41 1.4913281837399595 1105 42-43 1.5443389940217145 1105 44-45 1.7902580385680693 1105 46-47 0.891544782645326 1105 48-49 0.6474691063355422 1105 50-51 0.8826802822877369 1105 52-53 0.0032615898187700054 1105 54-55 -1.0571647150466532 1105 56-57 0.4756898510301255 1105 58-59 0.9023086388753043 1105 60-61 1.096438703688392 1105 62-63 1.0520962871090376 1105 64-65 0.02076673583010802 1105 66-67 1.5836115959748795 1105 68-69 1.6546276929256507 1105 70-71 1.7327904530075458 1105 72-73 0.5144672175965823 1105 74-75 -0.38222160270754557 1105 76 0.7163006748486858 1106 1 0.6032241630892834 1106 2 -0.20490644422730142 1106 3 -0.37108261007176324 1106 4 0.8347626176591056 1106 5 -0.39456692444904107 1106 6 -0.26756569110468575 1106 7 1.1618327705001477 1106 8 0.05478296996633247 1106 9 1.4594888735577243 1106 10-11 0.3278890906400065 1106 12-13 0.3755418437185227 1106 14-15 0.055536099020841334 1106 16-17 0.518171687617901 1106 18-19 0.7340537269942047 1106 20-21 0.23796116394891698 1106 22-23 -0.3272062251230565 1106 24-25 0.9031355445832858 1106 26-27 0.6436081869808916 1106 28-29 -1.5656252239691355 1106 30-31 -0.8079506042447946 1106 32-33 0.5202988975819451 1106 34-35 1.1059850544976726 1106 36-37 0.20735251582810577 1106 38-39 0.04839930143732829 1106 40-41 -0.28573115118723535 1106 42-43 0.864812804063007 1106 44-45 0.5195560172683571 1106 46-47 0.17811292000676815 1106 48-49 1.1137264416974233 1106 50-51 1.4105003214096712 1106 52-53 0.19039482172487254 1106 54-55 0.38491309412526675 1106 56-57 0.4709082818060395 1106 58-59 0.5348341768018443 1106 60-61 0.6092076669572464 1106 62-63 0.9267974390325335 1106 64-65 0.6355787332219833 1106 66-67 0.7242875359879193 1106 68-69 1.6088224331995065 1106 70-71 0.4482870841746873 1106 72-73 -0.1097449102017265 1106 74-75 -1.1480877187692684 1106 76 -0.6606825896590323 1107 1 -0.8655331777610797 1107 2 -0.6102388379317887 1107 3 0.23298128468980295 1107 4 -0.5224137976568421 1107 5 -0.10386925003043501 1107 6 -0.618399611706046 1107 7 -0.8791585379330229 1107 8 -1.0249680283893206 1107 9 -1.6119229187842983 1107 10-11 -1.2700084898157158 1107 12-13 -0.35067380110177027 1107 14-15 -1.2724530952474211 1107 16-17 -0.15953561799638294 1107 18-19 0.7349933558408139 1107 20-21 -0.16848703901091255 1107 22-23 -1.3613264976154227 1107 24-25 -1.6572943356140328 1107 26-27 -0.2854498351003869 1107 28-29 -0.9821744370299754 1107 30-31 -0.36949629369746617 1107 32-33 0.9844755478051042 1107 34-35 -0.04171285482814824 1107 36-37 -0.7800212215456312 1107 38-39 -0.050790268682469275 1107 40-41 0.14831864914163617 1107 42-43 0.5017637084557762 1107 44-45 0.6347780045833673 1107 46-47 0.0036943153556023844 1107 48-49 0.38522045156352647 1107 50-51 0.5761099056239019 1107 52-53 0.7066034193288218 1107 54-55 -0.3639710966193874 1107 56-57 0.669874690074785 1107 58-59 0.7463093940910213 1107 60-61 -0.4486382339119075 1107 62-63 -0.7760449382284449 1107 64-65 0.38968960942587927 1107 66-67 1.0031811230210437 1107 68-69 1.5564968518041553 1107 70-71 -0.08189849252251236 1107 72-73 1.1573445894459127 1107 74-75 -0.18232544486745894 1107 76 -0.5219698811788831 1108 1 -0.8928590682448636 1108 2 -0.47871305377442397 1108 3 -1.3109846908551184 1108 4 -0.5856780188157984 1108 5 0.19086759207482729 1108 6 0.3910634398622719 1108 7 0.4699111058734644 1108 8 0.4855051241890962 1108 9 -0.22765922925744775 1108 10-11 -0.2986102973648954 1108 12-13 -0.04636758100608063 1108 14-15 0.5067600892289263 1108 16-17 -0.6541661336795315 1108 18-19 0.23797049566005768 1108 20-21 0.25160865476899374 1108 22-23 -0.7352233609859695 1108 24-25 -0.477709008660284 1108 26-27 -0.2606757787473768 1108 28-29 0.3217064773772549 1108 30-31 0.06707999551041155 1108 32-33 0.3393931448279659 1108 34-35 0.4192653482553226 1108 36-37 0.45735251582810577 1108 38-39 0.5480933038853095 1108 40-41 0.10515012176258409 1108 42-43 0.33041867921600954 1108 44-45 -0.24751864613433838 1108 46-47 0.6510627364082353 1108 48-49 0.27963831440244036 1108 50-51 0.8850413250816374 1108 52-53 -0.010461971420774319 1108 54-55 -0.3051725851893039 1108 56-57 0.3950208889051865 1108 58-59 -0.23364807534013465 1108 60-61 0.7286079117552902 1108 62-63 0.2286334243446504 1108 64-65 0.5500830171877098 1108 66-67 -0.25264024858979894 1108 68-69 1.3196547465409978 1108 70-71 0.8277240486789736 1108 72-73 0.37201763569791524 1108 74-75 1.3907739703372215 1108 76 0.3438461741108547 1109 1 0.02270403305677604 1109 2 -0.3065568568304542 1109 3 0.6898076124838752 1109 4 0.3860254333630344 1109 5 0.47677591125988883 1109 6 0.8853475372023922 1109 7 0.16645892703927956 1109 8 1.4830150279808336 1109 9 1.7188037022648999 1109 10-11 1.2506822889395792 1109 12-13 1.2285050845287344 1109 14-15 0.6231154938695624 1109 16-17 0.012670312274060791 1109 18-19 0.529877682983205 1109 20-21 1.4590789433937772 1109 22-23 0.9340465880802498 1109 24-25 0.768414364288212 1109 26-27 1.0159512727523392 1109 28-29 0.6608688995617484 1109 30-31 0.6059028591210449 1109 32-33 0.6680858443186253 1109 34-35 0.2855924563481409 1109 36-37 0.5164923007743454 1109 38-39 0.8587893989617115 1109 40-41 0.4155066582651301 1109 42-43 0.4515844969862428 1109 44-45 0.6592159322471005 1109 46-47 -0.1038325663648294 1109 48-49 -0.5830102424736197 1109 50-51 0.9661392311370989 1109 52-53 1.1455086001694852 1109 54-55 0.43042447196972233 1109 56-57 0.7971148334439491 1109 58-59 0.548525099532533 1109 60-61 0.8982174193953654 1109 62-63 0.9246719076496888 1109 64-65 0.6381418740071823 1109 66-67 0.4122720321119502 1109 68-69 1.7070344862127627 1109 70-71 0.11295325071632334 1109 72-73 0.6744511388105252 1109 74-75 1.7657032289676664 1109 76 0.9928557949371175 1110 1 0.1432128187897952 1110 2 0.7599262273552263 1110 3 1.396075642906112 1110 4 1.1203553573074352 1110 5 0.6034478231402964 1110 6 0.1507553525708687 1110 7 0.6779983972726882 1110 8 1.918367768605016 1110 9 0.43254616227014964 1110 10-11 0.39708931752598886 1110 12-13 1.0074193252840402 1110 14-15 0.535258163683352 1110 16-17 0.2769635197222655 1110 18-19 1.0789204314752041 1110 20-21 0.3054597459114845 1110 22-23 0.9729923001180083 1110 24-25 1.3641395150881124 1110 26-27 -0.6177361125086094 1110 28-29 0.7579709189690362 1110 30-31 0.6154753742010399 1110 32-33 0.9698167648536398 1110 34-35 0.9704206755980707 1110 36-37 0.8658891011939573 1110 38-39 0.2897492730765805 1110 40-41 1.069912637808791 1110 42-43 0.6657203480221696 1110 44-45 1.284445410348333 1110 46-47 1.1134504129165776 1110 48-49 1.2037718188953832 1110 50-51 0.9188667422831784 1110 52-53 0.41373571792453134 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1.3543346706777442 2116 50-51 1.6139886935026908 2116 52-53 0.5061372188626265 2116 54-55 0.8453334876851955 2116 56-57 0.8418832370833229 2116 58-59 0.327991600773224 2116 60-61 0.22638119110752086 2116 62-63 0.5166901045065941 2116 64-65 0.1476538673901402 2116 66-67 0.8674002372401546 2116 68-69 1.450727621034929 2116 70-71 -1.6469723075963287 2116 72-73 0.6357828178841487 2116 74-75 -0.6011288636708798 2116 76 -0.027408886617887873 2117 1 0.3079665251503876 2117 2 -0.6506428783358302 2117 3 0.6815849685876074 2117 4 -1.4071434470923734 2117 5 -1.8881829755206319 2117 6 -0.48530394919861664 2117 7 -2.9195625783370645 2117 8 -1.3594897158521846 2117 9 -0.0028914273998665863 2117 10-11 -0.5073822271894528 2117 12-13 0.6573475892725611 2117 14-15 -0.21749171675662637 2117 16-17 0.1467626588901325 2117 18-19 -0.15924312663096885 2117 20-21 0.2414951356771482 2117 22-23 0.2809789093958699 2117 24-25 0.5726521884501352 2117 26-27 1.268323189260876 2117 28-29 0.751634237955173 2117 30-31 -0.0028890447372731387 2117 32-33 1.4179277165513895 2117 34-35 -0.21788635453106053 2117 36-37 -0.3465690527993459 2117 38-39 0.5713131181267954 2117 40-41 -1.6989778452136832 2117 42-43 -1.289085964746839 2117 44-45 0.18736267481509117 2117 46-47 0.6997727467281507 2117 48-49 0.28005111110007164 2117 50-51 1.3247730072281811 2117 52-53 1.6388672339455823 2117 54-55 -0.17710240975070235 2117 56-57 -0.6307789046964629 2117 58-59 -0.12921051084065027 2117 60-61 0.18980502217840467 2117 62-63 -0.31852859835916547 2117 64-65 0.6612285280236208 2117 66-67 -0.519100516907649 2117 68-69 0.8408105772943486 2117 70-71 1.4371151735499765 2117 72-73 1.6323891527257786 2117 74-75 1.0693085420606394 2117 76 0.38058508019961224 2118 1 1.5134567212288204 2118 2 1.7160237883308405 2118 3 1.1521732038817234 2118 4 1.4901114548684085 2118 5 0.8561307499695658 2118 6 1.7668529135464794 2118 7 1.5804374216629355 2118 8 -1.7987054021266928 2118 9 -0.4981855450469226 2118 10-11 0.8392844394772112 2118 12-13 1.902053471625507 2118 14-15 0.05623377343944469 2118 16-17 0.00793912947836617 2118 18-19 0.4642862851337384 2118 20-21 -0.6757597662836403 2118 22-23 0.6337240074350774 2118 24-25 1.0575541492344556 2118 26-27 1.796166326515781 2118 28-29 0.6806538457983109 2118 30-31 0.07181683761567115 2118 32-33 0.06518261851217488 2118 34-35 1.725054821939537 2118 36-37 1.1173525158281024 2118 38-39 1.0307248828326792 2118 40-41 -0.30642882560584184 2118 42-43 0.5862081529002197 2118 44-45 0.6293234591288268 2118 46-47 0.9736943153556012 2118 48-49 -1.2172037908607152 2118 50-51 -1.466011306497311 2118 52-53 -0.13309355036814452 2118 54-55 0.22956425691596394 2118 56-57 1.8676524678525581 2118 58-59 1.015299293080922 2118 60-61 -0.6882341935078671 2118 62-63 1.6928439506604391 2118 64-65 1.1553461750824496 2118 66-67 -1.061061301221379 2118 68-69 2.29649685180415 2118 70-71 -0.49543384605786756 2118 72-73 -0.051140259038930935 2118 74-75 0.2265634440214228 2118 76 0.9648988056898027 2119 1 -0.2823327524553889 2119 2 -1.378713053774426 2119 3 -2.3478267961182766 2119 4 -1.4540990714473807 2119 5 -1.13018503950412 2119 6 0.19632659775700745 2119 7 -1.630088894126537 2119 8 -0.16186329686353673 2119 9 0.5249723496899179 2119 10-11 -1.380189244733316 2119 12-13 -1.275314949427127 2119 14-15 -1.5748188581394942 2119 16-17 -0.675218765258478 2119 18-19 -0.6515031885504641 2119 20-21 -1.6589176610204817 2119 22-23 -0.56680230835439 2119 24-25 -1.0145511139234387 2119 26-27 -1.992254726115803 2119 28-29 -2.186188259464849 2119 30-31 -1.1723936887001258 2119 32-33 -0.7079752762246585 2119 34-35 -1.2596820201657337 2119 36-37 -1.6084369578561066 2119 38-39 -0.7150645908515258 2119 40-41 -1.6422182992900503 2119 42-43 -1.198528689205041 2119 44-45 -0.33172917245012457 2119 46-47 -2.3384109478022914 2119 48-49 -0.8177301066501954 2119 50-51 -0.2018007801815216 2119 52-53 -1.4209882872102533 2119 54-55 -1.8525410062419354 2119 56-57 -1.5523475321474436 2119 58-59 -0.9204901806032915 2119 60-61 -0.6187605092973385 2119 62-63 -0.4397876282869291 2119 64-65 -0.21570645649649833 2119 66-67 -0.5657981433266386 2119 68-69 -1.8382399903011049 2119 70-71 -0.4091180565841839 2119 72-73 -1.0332455221968253 2119 74-75 -1.5039628717680458 2119 76 0.3227935425319082 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 3.0 12 6.0 13 40.0 14 154.0 15 295.0 16 467.0 17 512.0 18 485.0 19 450.0 20 411.0 21 302.0 22 279.0 23 226.0 24 226.0 25 212.0 26 247.0 27 337.0 28 409.0 29 456.0 30 615.0 31 790.0 32 1064.0 33 1385.0 34 2117.0 35 3386.0 36 6169.0 37 7799.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.64109455167359 27.081079194443475 11.898363058881015 28.379463195001918 2 16.28874557936343 25.123084390818946 35.69100617155537 22.897163858262257 3 15.5467720685112 26.326190971499898 31.63442202343804 26.49261493655086 4 11.6600790513834 19.47160391096318 37.93426253380487 30.93405450384855 5 11.264822134387352 36.97385756882324 36.18334373483115 15.577976561958257 6 23.58712988003606 41.33208515359545 18.632549753831217 16.44823521253727 7 23.112128146453088 30.06032868733098 23.091325150821717 23.73621801539422 8 20.151168434921296 34.49136675681298 21.44095416406629 23.91651064419943 9 21.801539421676722 17.183274391512377 22.682199570071422 38.332986616739475 10-11 22.66139657444005 26.14936550863324 27.695721517231814 23.49351639969489 12-13 21.26412870119964 23.469246238124956 26.941612925594622 28.325012135080783 14-15 19.696969696969695 25.426461410443103 24.55620275986409 30.320366132723116 16-17 20.991956175022537 27.91068580542265 26.123361764094028 24.973996255460786 18-19 20.971153179391166 26.385132792455444 26.445808196380277 26.19790583177311 20-21 20.206643089938282 27.603841619859924 25.99334304139796 26.19617224880383 22-23 21.198252548366963 27.51369530545732 26.227376742250886 25.060675403924833 24-25 21.662852784134248 28.37355245822065 25.102281395187575 24.861313362457526 26-27 19.780181679495183 28.496636849039596 27.028292074058662 24.694889397406563 28-29 21.255460786353233 27.643714028153386 28.200194161292558 22.90063102420082 30-31 21.423618334373483 27.340337008529232 27.139241384092642 24.096803273004646 32-33 19.15262464461549 28.435961445114767 27.295263851327924 25.116150058941823 34-35 21.118507731780042 27.742528257402398 26.80986061992927 24.32910339088829 36-37 21.368143679356493 27.343804174467788 27.03869357187435 24.249358574301365 38-39 20.48401636502323 27.319534012897854 28.24526731849386 23.95118230358505 40-41 20.73365231259968 26.997087580611606 28.127383676582763 24.14187643020595 42-43 20.300256570279455 27.317800429928575 27.832674571805004 24.549268427986963 44-45 20.033284793010196 26.84626586228417 28.16205533596838 24.958394008737258 46-47 20.419873795159837 27.32646834477498 27.817072325081476 24.436585534983703 48-49 19.539213646765134 27.32820192774426 28.800013868663754 24.332570556826848 50-51 20.315858817002983 26.456209694195966 27.850010401497816 25.37792108730324 52-53 19.069412662090006 27.390610914638376 28.023368698425905 25.516607724845713 54-55 18.894320782192636 27.811871576173637 28.019901532487346 25.27390610914638 56-57 19.49240690659455 26.97281741904168 27.77719991678802 25.757575757575758 58-59 19.783648845433742 27.095901809860617 27.134040635184796 25.986408709520838 60-61 19.788849594341585 27.02135774218154 27.173913043478258 26.01587961999861 62-63 19.80098467512655 26.62090007627765 27.25712502600374 26.320990222592055 64-65 20.04715345676444 27.546633381873658 26.499549268427984 25.906663892933917 66-67 20.223978919631094 27.850010401497816 26.036682615629985 25.889328063241106 68-69 19.51320990222592 27.369807919007005 26.329658137438454 26.78732404132862 70-71 20.359198391235005 27.931488801054016 26.13029609597115 25.579016711739822 72-73 20.08182511615006 27.310866098051452 25.61195478815616 26.99535399764233 74-75 20.282920740586643 27.64544761112267 25.291241938839192 26.780389709451498 76 20.536023854101657 26.648637403786147 25.178559045835936 27.636779696276264 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 1.0 15 0.5 16 0.5 17 1.0 18 2.5 19 3.5 20 5.5 21 8.0 22 14.5 23 24.0 24 33.0 25 39.0 26 55.0 27 98.5 28 159.0 29 192.0 30 209.0 31 254.0 32 365.5 33 449.0 34 535.5 35 756.0 36 891.5 37 893.0 38 1034.0 39 1262.0 40 1510.0 41 1751.0 42 1831.0 43 1886.0 44 2062.5 45 2174.5 46 2165.0 47 2103.0 48 1984.0 49 1872.5 50 1818.0 51 1639.5 52 1315.0 53 1101.0 54 1033.0 55 951.0 56 758.5 57 551.0 58 454.0 59 387.0 60 285.0 61 220.5 62 191.0 63 161.0 64 107.0 65 77.5 66 63.0 67 54.0 68 44.0 69 31.5 70 25.5 71 22.0 72 18.0 73 11.0 74 11.0 75 14.0 76 9.0 77 5.0 78 4.0 79 2.0 80 2.5 81 2.5 82 2.5 83 3.0 84 1.5 85 0.0 86 0.5 87 1.0 88 0.5 89 0.5 90 1.0 91 1.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.5 98 1.0 99 1.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6622286942653075 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 28842.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.89092295957285 #Duplication Level Percentage of deduplicated Percentage of total 1 74.77810243122553 47.02863879065252 2 11.963173273058052 15.047500173358298 3 5.722476431997354 10.796754732681507 4 3.004575775952368 7.5584217460647665 5 1.5601742102651746 4.906039803064974 6 1.102596615028392 4.1605991262741835 7 0.6946358674678869 3.058040357811525 8 0.3969347814102211 1.997087580611608 9 0.23705827223110426 1.3417932182234242 >10 0.540272341363912 4.105124471257195 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0034671659385618198 0.0 4 0.0 0.0 0.0 0.0034671659385618198 0.0 5 0.0 0.0 0.0 0.0034671659385618198 0.0 6 0.0 0.0 0.0 0.0034671659385618198 0.0 7 0.0 0.0 0.0 0.0034671659385618198 0.0 8 0.0 0.0 0.0 0.0034671659385618198 0.0 9 0.0 0.0 0.0 0.02080299563137092 0.0 10 0.0 0.0 0.0 0.02427016156993274 0.0 11 0.0 0.0 0.0 0.03813882532418002 0.0 12 0.0 0.0 0.0 0.03813882532418002 0.0 13 0.0 0.0 0.0 0.03813882532418002 0.0 14 0.0 0.0 0.0 0.03813882532418002 0.0 15 0.0 0.0 0.0 0.03813882532418002 0.0 16 0.0 0.0 0.0 0.03813882532418002 0.0 17 0.0 0.0 0.0 0.03813882532418002 0.0 18 0.0 0.0 0.0 0.03813882532418002 0.0 19 0.0 0.0 0.0 0.04160599126274184 0.0 20 0.0 0.0 0.0 0.04507315720130366 0.0 21 0.0 0.0 0.0 0.04507315720130366 0.0 22 0.0 0.0 0.0 0.055474655016989116 0.0 23 0.0 0.0 0.0 0.055474655016989116 0.0 24 0.0 0.0 0.0 0.06587615283267458 0.0 25 0.0 0.0 0.0 0.0693433187712364 0.0 26 0.0 0.0 0.0 0.0693433187712364 0.0 27 0.0 0.0 0.0 0.09014631440260731 0.0 28 0.0 0.0 0.0 0.10054781221829277 0.0 29 0.0 0.0 0.0 0.11094931003397823 0.0 30 0.0 0.0 0.0 0.12828513972678732 0.0 31 0.0 0.0 0.0 0.16989113098952915 0.0 32 0.0 0.0 0.0 0.18029262880521463 0.0 33 0.0 0.0 0.0 0.22189862006795646 0.0 34 0.0 0.0 0.0 0.2669717772692601 0.0 35 0.0 0.0 0.0 0.3224464322862492 0.0 36 0.0 0.0 0.0 0.4541987379515984 0.0 37 0.0 0.0 0.0 0.5894182095555093 0.0 38 0.0 0.0 0.0 0.8113168296234657 0.0 39 0.0 0.0 0.0 1.1233617640940294 0.0 40 0.0 0.0 0.0 1.4284723666874697 0.0 41 0.0 0.0 0.0 1.7613202967894044 0.0 42 0.0 0.0 0.0 2.1427085500312044 0.0 43 0.0 0.0 0.0 2.5483669648429372 0.0 44 0.0 0.0 0.0 2.99216420497885 0.0 45 0.0 0.0 0.0 3.4706331045003815 0.0 46 0.0 0.0 0.0 4.053116982178767 0.0 47 0.0 0.0 0.0 4.555856043270231 0.0 48 0.0 0.0 0.0 5.117536925317245 0.0 49 0.0 0.0 0.0 5.554399833576035 0.0 50 0.0 0.0 0.0 5.9427224186949585 0.0 51 0.0 0.0 0.0 6.393453990707996 0.0 52 0.0 0.0 0.0 6.920463213369392 0.0 53 0.0 0.0 0.0 7.3469246238124954 0.0 54 0.0 0.0 0.0 7.7699188683170375 0.0 55 0.0 0.0 0.0 8.220650440330074 0.0 56 0.0 0.0 0.0 8.647111850773179 0.0 57 0.0 0.0 0.0 9.14638374592608 0.0 58 0.0 0.0 0.0 9.479231676028014 0.0 59 0.0 0.0 0.0 9.801678108314263 0.0 60 0.0 0.0 0.0 10.15532903404757 0.0 61 0.0 0.0 0.0 10.609527771999169 0.0 62 0.0 0.0 0.0 10.970113029609598 0.0 63 0.0 0.0 0.0 11.389640108175577 0.0 64 0.0 0.0 0.0 11.771028361417377 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGAGGG 15 0.0022033185 69.99652 49 TGGTTTA 20 0.00688511 52.497395 27 ACATGTC 20 0.00688511 52.497395 20 CCGCATT 20 0.00688511 52.497395 47 GTTCACC 20 0.00688511 52.497395 39 GACATGT 20 0.00688511 52.497395 19 ATTATCC 20 0.00688511 52.497395 31 ACTCAAC 20 0.00688511 52.497395 60 TACCGCA 20 0.00688511 52.497395 45 CTGTGGT 20 0.00688511 52.497395 9 ACAAGGA 20 0.00688511 52.497395 3 CGCATTC 20 0.00688511 52.497395 48 AGTTCAC 20 0.00688511 52.497395 38 CTACCGC 20 0.00688511 52.497395 44 ACTACCG 20 0.00688511 52.497395 43 TTCACCC 30 5.785341E-4 46.66435 40 GAGGCAA 65 4.655776E-7 37.690437 70 CGAGGCA 65 4.655776E-7 37.690437 69 ACGAGGC 65 4.655776E-7 37.690437 68 TGAAGAG 40 0.0023811918 34.998264 35 >>END_MODULE