FastQCFastQC Report
Wed 25 May 2016
SRR1780578_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780578_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28842
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT5391.8688024408848207Illumina PCR Primer Index 10 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT3511.2169752444351987No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC870.3016434366548783No Hit
CTTTACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC720.249635947576451Illumina PCR Primer Index 10 (95% over 21bp)
TATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCT370.12828513972678735Illumina PCR Primer Index 10 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC330.11441647597254005No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGAGC150.002202885570.015
ACGTCTC150.002202885570.058
GGCGTGA150.002202885570.024
ACCATGA150.002202885570.013
CCATGAG150.002202885570.014
GCGTGAA150.002202885570.025
TCTCGAG150.002202885570.061
TGCCACT150.002202885570.039
GTCTCGA150.002202885570.060
GCCACTA150.002202885570.040
CTGCCAC150.002202885570.038
CTCGAGT150.002202885570.062
ATGATTC200.006883760452.540
AATTTAC200.006883760452.553
TATGTCA200.006883760452.552
CGCCATT200.006883760452.517
ATTGGCG200.006883760452.521
CGGATGA200.006883760452.537
GATGATT200.006883760452.539
GCGGATG200.006883760452.536
Produced by FastQC (version 0.11.5)