##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780578_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 28842 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14094029540254 34.0 34.0 34.0 27.0 34.0 2 32.926010678871094 34.0 34.0 34.0 31.0 34.0 3 33.29210873032383 34.0 34.0 34.0 33.0 34.0 4 33.33777130573469 34.0 34.0 34.0 33.0 34.0 5 33.361001317523055 34.0 34.0 34.0 34.0 34.0 6 37.0195201442341 38.0 37.0 38.0 36.0 38.0 7 36.5713542750156 38.0 37.0 38.0 34.0 38.0 8 36.45392136467651 38.0 37.0 38.0 34.0 38.0 9 36.315338742112196 38.0 37.0 38.0 34.0 38.0 10-11 35.93622148256016 38.0 36.5 38.0 32.5 38.0 12-13 35.86384439359268 38.0 36.0 38.0 31.5 38.0 14-15 36.1072741141391 38.0 36.5 38.0 33.0 38.0 16-17 36.26957215172318 38.0 37.0 38.0 33.5 38.0 18-19 36.217495319325984 38.0 37.0 38.0 34.0 38.0 20-21 35.97732473476181 38.0 36.5 38.0 32.0 38.0 22-23 35.94842590666389 38.0 36.5 38.0 32.5 38.0 24-25 35.1812981069274 37.5 35.0 38.0 29.0 38.0 26-27 35.435597392691214 37.5 36.0 38.0 31.0 38.0 28-29 35.202586505790165 37.5 35.5 38.0 29.0 38.0 30-31 34.82950211497122 37.0 35.0 38.0 27.0 38.0 32-33 35.38501144164759 37.5 36.0 38.0 31.0 38.0 34-35 35.40302336869843 37.5 36.0 38.0 29.0 38.0 36-37 35.6101865335275 38.0 36.0 38.0 31.0 38.0 38-39 35.293651619166496 37.5 35.5 38.0 29.0 38.0 40-41 34.666285278413426 37.0 34.5 38.0 26.5 38.0 42-43 35.15033631509604 37.0 35.5 38.0 29.0 38.0 44-45 35.4156265168851 37.5 36.0 38.0 31.0 38.0 46-47 35.66037376048818 38.0 36.0 38.0 31.0 38.0 48-49 35.377279661604604 37.5 36.0 38.0 30.5 38.0 50-51 35.618906455862984 38.0 36.0 38.0 31.0 38.0 52-53 35.47729006310242 38.0 36.0 38.0 31.0 38.0 54-55 35.67826433673116 38.0 36.0 38.0 31.5 38.0 56-57 35.738783718188756 38.0 36.0 38.0 31.5 38.0 58-59 35.816552250190696 38.0 37.0 38.0 31.5 38.0 60-61 35.377557034879686 38.0 36.0 38.0 29.0 38.0 62-63 35.750398724082935 38.0 36.0 38.0 31.5 38.0 64-65 35.774166146591774 38.0 36.5 38.0 31.5 38.0 66-67 34.988350322446436 37.5 35.5 38.0 27.0 38.0 68-69 34.14293391581721 37.0 34.0 38.0 24.0 38.0 70-71 34.044535746480825 37.0 34.0 38.0 23.5 38.0 72-73 33.635618195686845 37.0 34.0 38.0 22.0 38.0 74-75 34.40896955828306 37.0 34.5 38.0 24.5 38.0 76 33.87972401359129 37.0 34.0 38.0 24.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.39883793075715 1101 2 0.6366221273060901 1101 3 0.6356429113075492 1101 4 0.7265754597685685 1101 5 0.6499560983567747 1101 6 -0.35174614065434184 1101 7 0.705628598791975 1101 8 0.8858331660383385 1101 9 0.6863500082105531 1101 10-11 0.3221306735024001 1101 12-13 1.0149598393683803 1101 14-15 0.8277833241599168 1101 16-17 0.2910869318789153 1101 18-19 0.037205560268255056 1101 20-21 0.14191340030994581 1101 22-23 0.8110684664099495 1101 24-25 -1.7477510408579704 1101 26-27 -0.12966787004886982 1101 28-29 1.285782868000723 1101 30-31 1.6419676130597551 1101 32-33 0.5196346923423292 1101 34-35 0.7803247361639976 1101 36-37 0.7854430579977816 1101 38-39 1.0594048624242305 1101 40-41 1.607349793837784 1101 42-43 1.5287836309361964 1101 44-45 0.3545515510334667 1101 46-47 0.8549455236705796 1101 48-49 -1.3265414883176092 1101 50-51 -1.8037730930832225 1101 52-53 -1.4964714740434246 1101 54-55 -0.19595503447278872 1101 56-57 -1.6751661339480677 1101 58-59 -0.40928837641205007 1101 60-61 -0.3817362485549012 1101 62-63 -0.19559948659323112 1101 64-65 -1.0606822353067713 1101 66-67 -1.3767746148475553 1101 68-69 -1.3069278574176195 1101 70-71 -0.3996048130683221 1101 72-73 -0.5383806843944541 1101 74-75 -0.15326815113808578 1101 76 -0.38406208372322226 1102 1 -0.5742614844475327 1102 2 0.40328879397276296 1102 3 0.16663706335432948 1102 4 0.5914877404703205 1102 5 0.15521925625151312 1102 6 0.2757392394626166 1102 7 0.6904239204294029 1102 8 0.6928507098979892 1102 9 0.7840108269240034 1102 10-11 0.7268090360755011 1102 12-13 0.9582346931695511 1102 14-15 0.289186832931847 1102 16-17 0.4864085693058229 1102 18-19 -0.2584084748194684 1102 20-21 0.5650128155146277 1102 22-23 1.0207175892169644 1102 24-25 -4.550382619805333 1102 26-27 -1.3583228408091017 1102 28-29 0.0790577218018953 1102 30-31 1.9019091335275888 1102 32-33 0.5491668560850158 1102 34-35 1.1145352624797837 1102 36-37 -1.6461358893706404 1102 38-39 0.3094048624242234 1102 40-41 1.557349793837787 1102 42-43 1.2784912332753748 1102 44-45 1.358645118284933 1102 46-47 1.5151794417992335 1102 48-49 -3.6224479210661507 1102 50-51 0.015232754869998644 1102 52-53 0.16405484174605078 1102 54-55 0.8540449655272084 1102 56-57 1.5184011175139176 1102 58-59 0.35591630195052915 1102 60-61 -5.632321043876541 1102 62-63 -2.1037866210961553 1102 64-65 0.43727098106749906 1102 66-67 1.37497977111736 1102 68-69 -0.004881073791892732 1102 70-71 -0.7472656317817794 1102 72-73 -5.998029807201469 1102 74-75 -2.0702272154655716 1102 76 0.713598734990228 1103 1 1.7062793170976143 1103 2 1.0748484270002834 1103 3 0.20159602037992386 1103 4 0.6131198022762092 1103 5 0.2646349974393445 1103 6 0.37414580633852523 1103 7 0.8112351227471208 1103 8 0.9677903525826821 1103 9 0.39328171055510097 1103 10-11 0.08900121376743897 1103 12-13 -0.07566197510664807 1103 14-15 0.09078026605593692 1103 16-17 0.5269686851918607 1103 18-19 0.7976031137850725 1103 20-21 -0.2412160594250139 1103 22-23 0.7411907905689645 1103 24-25 -2.5748152610414508 1103 26-27 -0.26860772733734706 1103 28-29 0.2653445397642358 1103 30-31 1.6386546670862572 1103 32-33 1.6477182805176582 1103 34-35 0.8349118921273018 1103 36-37 -0.6934560245710202 1103 38-39 0.12683605508478024 1103 40-41 0.3747809864983367 1103 42-43 0.953962020334771 1103 44-45 2.115815567343361 1103 46-47 1.4948028121517183 1103 48-49 -1.436837105035245 1103 50-51 0.1353196694040406 1103 52-53 0.7994000855896033 1103 54-55 0.16964129580244247 1103 56-57 1.0530703594260444 1103 58-59 0.672719778531885 1103 60-61 -4.027251029390786 1103 62-63 -0.8117055008643845 1103 64-65 1.0059334629603 1103 66-67 0.597384406559172 1103 68-69 0.9723280039483342 1103 70-71 0.9113533928440063 1103 72-73 -4.629512182865401 1103 74-75 -1.5864995476722328 1103 76 -2.921167078626379 1104 1 0.6148995369177648 1104 2 0.737942259319297 1104 3 0.20968031504792606 1104 4 -0.7846456623436424 1104 5 -0.5381627135244145 1104 6 0.15859489344907018 1104 7 0.8523832742595161 1104 8 1.1637209548172152 1104 9 1.1874501182215553 1104 10-11 -0.07808934849980176 1104 12-13 0.9955979031747617 1104 14-15 0.4696975155790568 1104 16-17 0.282396062792003 1104 18-19 0.5779646361758424 1104 20-21 -0.0023660276328456575 1104 22-23 0.7181641759809025 1104 24-25 1.0359123254786695 1104 26-27 1.3684619429324343 1104 28-29 1.824341723886313 1104 30-31 1.6620446207605255 1104 32-33 1.374805209394026 1104 34-35 1.2758692906194398 1104 36-37 1.6587103847304547 1104 38-39 1.3331672386618507 1104 40-41 2.279131972055602 1104 42-43 1.0039211446875669 1104 44-45 1.3630223981181757 1104 46-47 1.7617662057387875 1104 48-49 0.444305596390862 1104 50-51 0.804862770503135 1104 52-53 1.3426374368476601 1104 54-55 0.622361797210381 1104 56-57 0.4630076834336734 1104 58-59 -0.44663711128553985 1104 60-61 0.3512120462745827 1104 62-63 0.9577558489403231 1104 64-65 1.4746863015469103 1104 66-67 0.7946775303669682 1104 68-69 2.6359214275108727 1104 70-71 2.5502851759305685 1104 72-73 1.7233354872227125 1104 74-75 0.3423314088179126 1104 76 0.39030535302045166 1105 1 1.1473322194383506 1105 2 0.3621672986456588 1105 3 0.518924323560924 1105 4 0.12439477441031244 1105 5 0.18266637873060887 1105 6 0.20412094138096393 1105 7 0.7344967192488809 1105 8 -0.3527961174507581 1105 9 0.928821451616578 1105 10-11 0.5437298427651243 1105 12-13 -0.3357151346710836 1105 14-15 0.5411789627892034 1105 16-17 0.11995505233582548 1105 18-19 0.7691370244427063 1105 20-21 0.5359424761147267 1105 22-23 1.0056167530143085 1105 24-25 1.4069218563382933 1105 26-27 0.5324297415814598 1105 28-29 0.8552013519051869 1105 30-31 1.565903230920604 1105 32-33 1.528409354855306 1105 34-35 -0.20705844140609742 1105 36-37 1.4994617495865725 1105 38-39 -0.019567100192595888 1105 40-41 0.2237049340247026 1105 42-43 0.5638822809881177 1105 44-45 1.0211143755402219 1105 46-47 1.4993899681150253 1105 48-49 0.5129185324508256 1105 50-51 1.2956557750372326 1105 52-53 1.0105378717509694 1105 54-55 1.1689982365552467 1105 56-57 0.9231723914932601 1105 58-59 0.6959556526637556 1105 60-61 1.2571526403339846 1105 62-63 0.2039851447671026 1105 64-65 0.9284662589819064 1105 66-67 0.7701101203549356 1105 68-69 0.5740171062376263 1105 70-71 1.7537181360490166 1105 72-73 2.275014954234827 1105 74-75 1.2646965425275454 1105 76 1.682708425103364 1106 1 -0.3900509581317415 1106 2 0.3311957707169455 1106 3 -0.1475612230593768 1106 4 0.15525763031120476 1106 5 -0.39655552955019857 1106 6 -0.5411518254088605 1106 7 -0.9669812203294725 1106 8 0.5028874296042289 1106 9 0.06748695911494451 1106 10-11 0.9877637484377999 1106 12-13 1.7674146197301397 1106 14-15 -0.03461977661527271 1106 16-17 0.4824306011295647 1106 18-19 0.6519342424387915 1106 20-21 -0.2494302689406993 1106 22-23 0.6641692416037444 1106 24-25 -0.4989138315556403 1106 26-27 0.1375155924834317 1106 28-29 0.6905632297578279 1106 30-31 1.1180658042742238 1106 32-33 0.722606268569713 1106 34-35 1.2256735733732924 1106 36-37 1.2063732905559235 1106 38-39 1.0582420717265606 1106 40-41 1.027117235698249 1106 42-43 1.4395071451480845 1106 44-45 1.4323293288112495 1106 46-47 1.423808572766191 1106 48-49 1.8849572196927227 1106 50-51 0.8189659250046333 1106 52-53 1.0267843399100656 1106 54-55 0.256370546922561 1106 56-57 0.9212162949925045 1106 58-59 0.9121586520117262 1106 60-61 1.3152921752177065 1106 62-63 1.2209379811070278 1106 64-65 -0.018175775358457713 1106 66-67 1.3216749975555473 1106 68-69 2.2703331244945275 1106 70-71 1.4146070732365814 1106 72-73 1.7631697032024434 1106 74-75 0.7524166033838782 1106 76 1.445912076483502 1107 1 1.4735854055046254 1107 2 -0.17044857976461714 1107 3 0.0679661436307839 1107 4 0.6386966718897753 1107 5 -0.2793368309361526 1107 6 0.24320335429515438 1107 7 0.426840720004094 1107 8 -0.44143956123438954 1107 9 -0.32880150694096244 1107 10-11 0.45041350178522777 1107 12-13 0.013949738358277841 1107 14-15 0.16616716254375774 1107 16-17 0.6001778409698275 1107 18-19 0.9215489946116904 1107 20-21 0.9030245114210587 1107 22-23 -0.3146891093476256 1107 24-25 0.43255198944506645 1107 26-27 -0.4195668599478566 1107 28-29 1.2610353932532448 1107 30-31 0.4980282191203571 1107 32-33 1.6443821670897947 1107 34-35 0.6081025139417733 1107 36-37 1.0409986135533416 1107 38-39 0.6993038523232187 1107 40-41 0.5179558544438478 1107 42-43 0.8510058531584193 1107 44-45 1.1495010459829658 1107 46-47 1.2115111802362364 1107 48-49 0.7714383096621873 1107 50-51 0.8300652907551651 1107 52-53 0.1424174148454611 1107 54-55 0.20151971300195726 1107 56-57 0.6591773003953705 1107 58-59 1.15182273469906 1107 60-61 0.9591728423541852 1107 62-63 0.6670267760330333 1107 64-65 0.4812369566124204 1107 66-67 0.24797285989992446 1107 68-69 1.082466081976321 1107 70-71 1.0998901364266231 1107 72-73 1.339397093383326 1107 74-75 -0.8653893632592968 1107 76 1.0088672092060733 1108 1 -1.1426825370791107 1108 2 0.21381510976223694 1108 3 0.008742326512226839 1108 4 0.6020140562597973 1108 5 -0.09741232269585964 1108 6 -0.32426076053738484 1108 7 -0.5727339743074396 1108 8 -0.40188613220727376 1108 9 -0.4265154888654692 1108 10-11 -1.0679278060297648 1108 12-13 1.3634978510642881 1108 14-15 0.5891868329318442 1108 16-17 -0.6662230096415556 1108 18-19 0.5258020514963206 1108 20-21 1.1439601839356826 1108 22-23 0.11545443132223454 1108 24-25 1.5654068538788763 1108 26-27 -0.9372702092301566 1108 28-29 -1.0525212255665295 1108 30-31 0.4229617651065354 1108 32-33 0.8754826455586979 1108 34-35 0.20400894669030833 1108 36-37 1.1854430579977873 1108 38-39 0.9304574940031713 1108 40-41 0.6784024254167349 1108 42-43 -0.5636140298825154 1108 44-45 1.2323293288112467 1108 46-47 0.5730741786413418 1108 48-49 1.2091310263022734 1108 50-51 0.6468117022384163 1108 52-53 -0.03594515825395206 1108 54-55 -0.8091129292096326 1108 56-57 0.6236642754086574 1108 58-59 1.2559163019505206 1108 60-61 1.2203105350708228 1108 62-63 1.0804239052196323 1108 64-65 0.5372709810674934 1108 66-67 0.7855060869068282 1108 68-69 1.2688031367344266 1108 70-71 -1.0156866844133532 1108 72-73 -0.5769771756225239 1108 74-75 -0.5439114259918867 1108 76 1.0188618928849706 1109 1 -0.4814488075941057 1109 2 0.6979124498867364 1109 3 0.48797266041149356 1109 4 0.2652851371879237 1109 5 0.06931093706645441 1109 6 -0.6101690797224393 1109 7 -0.13164738687110855 1109 8 0.8234675746404889 1109 9 0.8279270691424543 1109 10-11 0.2690840785203221 1109 12-13 0.019977760515338616 1109 14-15 0.8492887005040046 1109 16-17 1.028004494602932 1109 18-19 0.637743194676851 1109 20-21 1.0248883107042133 1109 22-23 0.8901007244744648 1109 24-25 0.2732167010775228 1109 26-27 0.40545757797980997 1109 28-29 -1.0312422216050052 1109 30-31 0.7754801578626243 1109 32-33 1.0818210722706354 1109 34-35 0.5453784996048583 1109 36-37 0.9746903698257441 1109 38-39 0.6395123893059491 1109 40-41 0.430468073407674 1109 42-43 1.3689270001118246 1109 44-45 1.2280282535424334 1109 46-47 0.8918630863945936 1109 48-49 0.5953223109655426 1109 50-51 0.08421973845800323 1109 52-53 0.33417368724689567 1109 54-55 0.31426001929064995 1109 56-57 -0.22857115186921817 1109 58-59 -0.022012390748969324 1109 60-61 0.8001633930221601 1109 62-63 -0.0597571926864191 1109 64-65 0.749891241395737 1109 66-67 0.14419312708792376 1109 68-69 0.27927285942826074 1109 70-71 0.15935576040837418 1109 72-73 0.997103186573284 1109 74-75 -0.24108177120977103 1109 76 -0.4704420120386317 1110 1 -2.2193192508146673 1110 2 -1.2235404743199219 1110 3 -0.5071755168767353 1110 4 -0.28887169470297636 1110 5 0.19873658616165102 1110 6 -0.736840991602854 1110 7 0.4533250757567444 1110 8 1.0703860115667965 1110 9 1.1077592223026969 1110 10-11 0.3158976111717777 1110 12-13 0.8920601103710908 1110 14-15 1.3176207225338956 1110 16-17 0.7534175551851519 1110 18-19 -0.2648269600569506 1110 20-21 0.9673876550524909 1110 22-23 -0.070232346598182 1110 24-25 -0.43921445549211313 1110 26-27 1.333204758677418 1110 28-29 0.6958099682717247 1110 30-31 0.512563819021814 1110 32-33 0.969770193697336 1110 34-35 1.4705686386030195 1110 36-37 1.6988576921441236 1110 38-39 0.6728194965705683 1110 40-41 -1.926796547625628 1110 42-43 0.38420368513673964 1110 44-45 0.8225732312502743 1110 46-47 0.700609480310149 1110 48-49 1.867089947996746 1110 50-51 -0.07071076245991037 1110 52-53 0.8023090137614517 1110 54-55 0.2589230143076975 1110 56-57 1.118465302366289 1110 58-59 -0.9057653411816915 1110 60-61 0.12300629887056402 1110 62-63 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-0.4483945509665048 2116 54-55 -1.5459550344727901 2116 56-57 0.08915010536816936 2116 58-59 -1.6665533336770082 2116 60-61 -2.1274627442814023 2116 62-63 0.04115265015890657 2116 64-65 -2.0611095857341297 2116 66-67 -0.9703643584373012 2116 68-69 -1.4295774300671908 2116 70-71 0.04676270829919815 2116 72-73 0.07443982842605834 2116 74-75 0.7206634727935395 2116 76 -1.0169680666292038 2117 1 0.5609294340251232 2117 2 0.6150534998551151 2117 3 0.5872768982356504 2117 4 -0.3773461088588874 2117 5 -0.38925958791773496 2117 6 -0.06873960470662865 2117 7 -0.1126720548028004 2117 8 0.5407351268226535 2117 9 -0.7678983578025154 2117 10-11 -1.0576078885891036 2117 12-13 -2.741249310958416 2117 14-15 -0.5349617738793029 2117 16-17 -1.6291745060295852 2117 18-19 -2.042206204437626 2117 20-21 -0.22115849511489216 2117 22-23 0.31989199582171324 2117 24-25 -0.6663784918383513 2117 26-27 -0.37411231449330984 2117 28-29 -2.6890537333064657 2117 30-31 -0.7583591843258688 2117 32-33 -1.9643522357622558 2117 34-35 0.5754227753796783 2117 36-37 -2.651811843962996 2117 38-39 -1.2317716081640029 2117 40-41 -0.6014737355739825 2117 42-43 -1.318928787364456 2117 44-45 -0.8912000829534605 2117 46-47 -0.7947276789438007 2117 48-49 -1.4056264556378721 2117 50-51 -0.5995247270701469 2117 52-53 -0.8641185328669536 2117 54-55 -0.5851707207472998 2117 56-57 -0.31111384636636785 2117 58-59 -2.3056936051702195 2117 60-61 -2.4389257910385638 2117 62-63 -2.994292297050748 2117 64-65 -1.119178967332914 2117 66-67 -1.6189314775926533 2117 68-69 -1.9974507332346114 2117 70-71 -1.081630956859172 2117 72-73 0.22828598227221164 2117 74-75 -0.28725507924265514 2117 76 0.21266994242056825 2118 1 -0.9500509581317402 2118 2 -0.2367112060272376 2118 3 -1.9154681998035628 2118 4 0.05990879310189712 2118 5 -0.290043901643223 2118 6 0.07047608156788243 2118 7 0.8767397099030845 2118 8 0.7191664993716742 2118 9 -1.2380944362338866 2118 10-11 -0.9589804376087088 2118 12-13 -1.942817938409398 2118 14-15 0.26444999082657716 2118 16-17 -3.0478019570099733 2118 18-19 -0.7794611063984078 2118 20-21 -0.3591977108011619 2118 22-23 -0.005598200256713426 2118 24-25 0.03224895914203785 2118 26-27 -3.2741123144933084 2118 28-29 -1.131994909777056 2118 30-31 0.08085650194863803 2118 32-33 -0.5992541965465605 2118 34-35 -1.1596752638360073 2118 36-37 -3.594556942002214 2118 38-39 -0.6405951375757724 2118 40-41 -0.11265020616221477 2118 42-43 1.2132280753806413 2118 44-45 -3.67767067118875 2118 46-47 0.09938996811502676 2118 48-49 -1.4287637105398332 2118 50-51 -1.9626619819721114 2118 52-53 -2.396471474043423 2118 54-55 -1.5359550344727921 2118 56-57 -0.5073883561702957 2118 58-59 -1.5803994875231666 2118 60-61 -1.5228473596660166 2118 62-63 -1.3111550421487834 2118 64-65 -0.009571124195659308 2118 66-67 0.6565587184857762 2118 68-69 -1.728038968528736 2118 70-71 -0.07516036862388376 2118 72-73 1.7482859822722077 2118 74-75 0.0545096266396925 2118 76 -1.0885065281676631 2119 1 1.0310016734472036 2119 2 0.5717098466043424 2119 3 -0.09125767348777458 2119 4 0.233593003628215 2119 5 -0.19214916480112265 2119 6 -0.3189976026426464 2119 7 -1.1622076585179713 2119 8 -0.6439913953651697 2119 9 -0.8370418046549446 2119 10-11 -0.3047699112929152 2119 12-13 -0.4259758331462393 2119 14-15 -0.8739710618049941 2119 16-17 -1.789907220167862 2119 18-19 -1.6268295274510436 2119 20-21 -1.713934552906423 2119 22-23 -1.484545568677774 2119 24-25 0.23119632756309016 2119 26-27 -0.43990178817751513 2119 28-29 -1.2656791203033677 2119 30-31 0.4387512387907506 2119 32-33 -0.3376752491781403 2119 34-35 1.0408510519534673 2119 36-37 -0.13297799463379079 2119 38-39 -1.961647769154716 2119 40-41 -0.2926502061622145 2119 42-43 -0.7294035035667292 2119 44-45 -0.5413548817150655 2119 46-47 -2.040083716095502 2119 48-49 -1.3540268684345733 2119 50-51 0.10996959697526165 2119 52-53 -1.062260947727637 2119 54-55 0.4277291760535249 2119 56-57 -2.0500199351176605 2119 58-59 -1.8993468559442164 2119 60-61 1.2045210613866146 2119 62-63 -0.006418200043526667 2119 64-65 -1.1258869136693477 2119 66-67 0.5144534553278888 2119 68-69 -0.13909160010767607 2119 70-71 -1.0630551054659918 2119 72-73 0.10986492964063643 2119 74-75 -2.3886482680971497 2119 76 -1.7548223176413487 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 2.0 22 5.0 23 13.0 24 16.0 25 47.0 26 77.0 27 134.0 28 260.0 29 415.0 30 668.0 31 1009.0 32 1650.0 33 2416.0 34 3483.0 35 4737.0 36 6723.0 37 7187.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.002496359475764 26.745718050065875 11.37577144442133 27.876014146037033 2 16.905901116427433 20.608834338811455 39.86894112752236 22.61632341723875 3 16.25060675403925 27.893349975729837 31.506136883711257 24.34990638651966 4 12.374315234727135 20.626170168504267 39.1477706123015 27.8517439844671 5 11.857707509881422 39.220581097011305 36.287358712988 12.634352680119271 6 25.514874141876433 40.024963594757644 18.37251230843908 16.087649954926842 7 23.788225504472642 32.560155329034046 23.653006032868735 19.998613133624577 8 21.11157339990292 36.66181263435268 22.436030788433534 19.790583177310868 9 22.67179807225574 15.50863324318702 23.01851466611192 38.80105401844533 10-11 23.555925386589003 25.332847930101938 28.860689272588587 22.25053741072048 12-13 23.25254836696484 22.31294639761459 26.906941266209 27.527563969211567 14-15 21.186117467582 25.587684626586228 23.70327993897788 29.522917966853896 16-17 22.63712641287012 28.101379932043546 25.067609735801955 24.193883919284374 18-19 22.517509187989738 27.473822897163856 25.095347063310452 24.913320851535957 20-21 21.404548921711395 27.244989945218776 25.358851674641148 25.991609458428684 22-23 22.314679980583872 28.330212883988626 26.63996948893974 22.71513764648776 24-25 22.15865751334859 29.410235073850632 25.596352541432633 22.834754871368144 26-27 22.264406074474724 29.522917966853896 26.112960266278346 22.099715692393037 28-29 20.709382151029747 28.79828028569447 28.33368004992719 22.15865751334859 30-31 22.31814714652243 28.191526246446152 27.87948131197559 21.61084529505582 32-33 21.446154912974137 28.255668816309548 28.690798141599057 21.60737812911726 34-35 21.040496498162405 28.385687539005616 29.446640316205535 21.127175646626448 36-37 21.80500658761528 26.77692254351293 28.364884543374245 23.05318632549754 38-39 22.19852992164205 26.442341030441717 27.68185285347757 23.677276194438665 40-41 20.5048193606546 27.03869357187435 29.249011857707508 23.20747520976354 42-43 22.954372096248527 26.25164690382082 29.13806254767353 21.655918452257126 44-45 20.152902017890575 26.749185216004438 28.48623535122391 24.611677414881076 46-47 22.784480965258997 25.77491158726857 28.193259829415435 23.247347618057002 48-49 20.589764926149364 25.83038624228556 28.649192150336315 24.930656681228765 50-51 19.783648845433742 25.941335552319533 29.406767907912073 24.86824769433465 52-53 20.662575410859162 25.965605713889467 30.92365300603287 22.4481658692185 54-55 19.589487552874278 26.6070314125234 30.039525691699602 23.76395534290271 56-57 21.220789126967617 25.811316829623465 29.249011857707508 23.718882185701407 58-59 19.582553220997156 28.4324942791762 29.144996879550654 22.839955620275987 60-61 20.00728104847098 30.433049025726373 26.425005200748906 23.134664725053742 62-63 18.396782470009015 31.357048748353094 26.81332778586783 23.432840995770057 64-65 19.117952985229874 31.259968102073366 25.941335552319533 23.680743360377228 66-67 18.911656611885444 31.78697732473476 25.478468899521534 23.82289716385826 68-69 19.369322515775604 30.580403578115252 25.80958324665418 24.240690659454962 70-71 19.495874072533113 30.72602454753485 25.433395742320226 24.344705637611817 72-73 19.440399417516122 29.09298939047223 25.62755703487969 25.839054157131958 74-75 19.651896539768394 29.57839262187088 25.561680882047018 25.20802995631371 76 19.530545731918732 30.323833298661672 25.324180015255532 24.821440954164068 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 1.0 18 2.5 19 2.5 20 6.0 21 11.0 22 14.5 23 22.5 24 34.0 25 41.0 26 58.5 27 94.5 28 130.0 29 147.0 30 203.5 31 287.5 32 388.5 33 462.0 34 535.5 35 730.5 36 908.0 37 964.0 38 1189.5 39 1619.0 40 1991.0 41 2154.5 42 2150.0 43 2144.0 44 2139.5 45 2226.0 46 2311.0 47 2240.0 48 1963.0 49 1662.0 50 1567.0 51 1456.5 52 1142.0 53 891.5 54 845.0 55 753.5 56 575.5 57 431.0 58 373.0 59 312.0 60 196.0 61 132.5 62 124.0 63 86.5 64 45.5 65 30.0 66 17.5 67 17.0 68 12.5 69 6.5 70 3.5 71 2.0 72 1.0 73 0.0 74 0.5 75 1.0 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 28842.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.210595659108236 #Duplication Level Percentage of deduplicated Percentage of total 1 67.00983905649312 35.65633451216975 2 14.862839642926955 15.81721101171902 3 7.91034078321496 12.627418348242148 4 3.994265980321887 8.501490881353583 5 2.1828370365543757 5.807502947091048 6 1.342281879194631 4.285417100062409 7 0.81449143155014 3.0337701962415924 8 0.5864338307161009 2.49635947576451 9 0.3974718185964683 1.903474100270439 >10 0.8731348146217501 6.233964357534152 >50 0.013031862904802242 0.5512793842313293 >100 0.006515931452401121 1.2169752444351987 >500 0.006515931452401121 1.8688024408848207 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT 539 1.8688024408848207 Illumina PCR Primer Index 10 (95% over 23bp) CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT 351 1.2169752444351987 No Hit ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 87 0.3016434366548783 No Hit CTTTACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC 72 0.249635947576451 Illumina PCR Primer Index 10 (95% over 21bp) TATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCT 37 0.12828513972678735 Illumina PCR Primer Index 10 (95% over 22bp) CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC 33 0.11441647597254005 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0034671659385618198 0.0 4 0.0 0.0 0.0 0.0034671659385618198 0.0 5 0.0 0.0 0.0 0.0034671659385618198 0.0 6 0.0 0.0 0.0 0.0034671659385618198 0.0 7 0.0 0.0 0.0 0.0034671659385618198 0.0 8 0.0 0.0 0.0 0.0034671659385618198 0.0 9 0.0 0.0 0.0 0.02080299563137092 0.0 10 0.0 0.0 0.0 0.02427016156993274 0.0 11 0.0 0.0 0.0 0.03813882532418002 0.0 12 0.0 0.0 0.0 0.03813882532418002 0.0 13 0.0 0.0 0.0 0.03813882532418002 0.0 14 0.0 0.0 0.0 0.03813882532418002 0.0 15 0.0 0.0 0.0 0.03813882532418002 0.0 16 0.0 0.0 0.0 0.03813882532418002 0.0 17 0.0 0.0 0.0 0.03813882532418002 0.0 18 0.0 0.0 0.0 0.04160599126274184 0.0 19 0.0 0.0 0.0 0.052007489078427296 0.0 20 0.0 0.0 0.0 0.055474655016989116 0.0 21 0.0 0.0 0.0 0.055474655016989116 0.0 22 0.0 0.0 0.0 0.055474655016989116 0.0 23 0.0 0.0 0.0 0.058941820955550936 0.0 24 0.0 0.0 0.0 0.062408986894112756 0.0 25 0.0 0.0 0.0 0.06587615283267458 0.0 26 0.0 0.0 0.0 0.06587615283267458 0.0 27 0.0 0.0 0.0 0.0866791484640455 0.0 28 0.0 0.0 0.0 0.09708064627973095 0.0 29 0.0 0.0 0.0 0.11441647597254005 0.0 30 0.0 0.0 0.0 0.12828513972678732 0.0 31 0.0 0.0 0.0 0.16295679911240551 0.0 32 0.0 0.0 0.0 0.16989113098952915 0.0 33 0.0 0.0 0.0 0.211497122252271 0.0 34 0.0 0.0 0.0 0.24963594757645102 0.0 35 0.0 0.0 0.0 0.29817627071631647 0.0 36 0.0 0.0 0.0 0.422994244504542 0.0 37 0.0 0.0 0.0 0.5512793842313293 0.0 38 0.0 0.0 0.0 0.7593093405450385 0.0 39 0.0 0.0 0.0 1.0505512793842313 0.0 40 0.0 0.0 0.0 1.3175230566534915 0.0 41 0.0 0.0 0.0 1.6607724845711116 0.0 42 0.0 0.0 0.0 2.031759239997226 0.0 43 0.0 0.0 0.0 2.423548991054712 0.0 44 0.0 0.0 0.0 2.8534775674363777 0.0 45 0.0 0.0 0.0 3.290340475695167 0.0 46 0.0 0.0 0.0 3.8312183621108105 0.0 47 0.0 0.0 0.0 4.299285763816656 0.0 48 0.0 0.0 0.0 4.833229318355176 0.0 49 0.0 0.0 0.0 5.277026558491089 0.0 50 0.0 0.0 0.0 5.634144650162956 0.0 51 0.0 0.0 0.0 6.0848762221759936 0.0 52 0.0 0.0 0.0 6.601483947021705 0.0 53 0.0 0.0 0.0 7.014076693710561 0.0 54 0.0 0.0 0.0 7.423202274460856 0.0 55 0.0 0.0 0.0 7.839262187088274 0.0 56 0.0 0.0 0.0 8.248387767838569 0.0 57 0.0 0.0 0.0 8.737258165175785 0.0 58 0.0 0.0 0.0 9.03196726995354 0.0 59 0.0 0.0 0.0 9.344012204424104 0.0 60 0.0 0.0 0.0 9.697663130157409 0.0 61 0.0 0.0 0.0 10.148394702170446 0.0 62 0.0 0.0 0.0 10.502045627903751 0.0 63 0.0 0.0 0.0 10.914638374592608 0.0 64 0.0 0.0 0.0 11.306428125650093 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATGAGC 15 0.0022028855 70.0 15 ACGTCTC 15 0.0022028855 70.0 58 GGCGTGA 15 0.0022028855 70.0 24 ACCATGA 15 0.0022028855 70.0 13 CCATGAG 15 0.0022028855 70.0 14 GCGTGAA 15 0.0022028855 70.0 25 TCTCGAG 15 0.0022028855 70.0 61 TGCCACT 15 0.0022028855 70.0 39 GTCTCGA 15 0.0022028855 70.0 60 GCCACTA 15 0.0022028855 70.0 40 CTGCCAC 15 0.0022028855 70.0 38 CTCGAGT 15 0.0022028855 70.0 62 ATGATTC 20 0.0068837604 52.5 40 AATTTAC 20 0.0068837604 52.5 53 TATGTCA 20 0.0068837604 52.5 52 CGCCATT 20 0.0068837604 52.5 17 ATTGGCG 20 0.0068837604 52.5 21 CGGATGA 20 0.0068837604 52.5 37 GATGATT 20 0.0068837604 52.5 39 GCGGATG 20 0.0068837604 52.5 36 >>END_MODULE