FastQCFastQC Report
Wed 25 May 2016
SRR1780577_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780577_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences295573
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCGTCCTTTAGTGTTGTGTATGGTTATTATTTGTTTTGAGGTTAGTTT5380.18201933194168615No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGT207.832099E-444.0169435
CTACGTC207.832099E-444.0169436
CGCTTGA207.838639E-444.00948310
TGGACCA207.884535E-443.957365
GAATCGC207.884535E-443.957366
TCGCTTG207.884535E-443.957369
GAATATC250.002331867835.25536715
GTACAAG505.9177182E-835.2016221
AATCGGG250.002349390635.20162223
AGAGCGC250.002361130235.1658868
CCGACGT353.195228E-431.45666313
CTATACA353.2111493E-431.4300231
GGAATCG300.00569415429.37947321
CGTAGCG300.00569415429.37947315
CCGAAAT300.005727284629.3446324
TTGGTCG300.005732029729.33965910
ACAGACA300.005736778529.3346881
CGGTAAG300.00575580329.31482728
GACTCGA300.00575580329.31482729
CCCGTGC454.0862047E-529.3049078
Produced by FastQC (version 0.11.5)