FastQCFastQC Report
Wed 25 May 2016
SRR1780577_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780577_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences295573
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT110583.7412077557828356No Hit
ATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTCTT46571.5755836967517332No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC13360.45200339679199386No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC9120.30855321697178023No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG8370.2831787747866009No Hit
CTTTACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTC5220.1766061176088479No Hit
TATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTCT3380.11435415278120804No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGA207.8465324E-444.00050710
AGACGGT207.8465324E-444.00050710
GACGGTT207.8465324E-444.00050711
CCGAAGT207.8465324E-444.00050726
CCGCGCT207.8465324E-444.00050720
CCGTCTT6050.037.4549844
GCCGTCT6250.036.25641643
CGAGAAT250.00234784335.20636414
GCCCAGT250.002349793435.20040519
CCGTGCG250.002349793435.2004059
AGACCGC250.002349793435.20040510
TCCGTGC250.002351745335.1944537
ACATGTT250.002351745335.1944529
TTCAGCC1600.034.37539744
ATGATTC1600.034.36958340
TGGCGTG1700.032.35331323
GCGTGAA1700.032.35331325
AGCGGAT1700.032.34784335
CGGATGA1700.032.34784337
GCGGATG1700.032.34784336
Produced by FastQC (version 0.11.5)