FastQCFastQC Report
Wed 25 May 2016
SRR1780576_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780576_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5028
Sequences flagged as poor quality0
Sequence length76
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTG70.13922036595067622No Hit
CCTTTGAAATCTGTTACCAGGTGATAGGTTACACACTGTCTCTTATACAC60.11933174224343676No Hit
TATGTAAATTATATAAATCTGAGATAAAATAAAGGCATTTCATAACCTAA60.11933174224343676No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGCC500.006555202428.00000213
Produced by FastQC (version 0.11.5)