##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780576_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5028 Sequences flagged as poor quality 0 Sequence length 76 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.071797931583134 21.0 12.0 27.0 12.0 34.0 2 20.661296738265712 21.0 11.0 31.0 11.0 34.0 3 20.283412887828163 21.0 11.0 31.0 11.0 34.0 4 21.065234685759744 21.0 11.0 32.0 11.0 34.0 5 20.305091487669053 21.0 11.0 31.0 11.0 34.0 6 22.91686555290374 24.0 11.0 34.0 11.0 37.0 7 23.28918058870326 26.0 11.0 34.0 11.0 37.0 8 23.70783611774065 26.0 11.0 34.0 11.0 37.0 9 23.43774860779634 24.0 12.0 34.0 12.0 37.0 10-11 22.042959427207638 21.0 11.0 34.0 11.0 37.0 12-13 21.98070803500398 21.5 11.0 33.5 11.0 37.0 14-15 21.534108989657916 21.0 11.0 32.0 11.0 37.0 16-17 21.08581941129674 21.0 11.0 31.0 11.0 37.0 18-19 21.38713206046142 21.0 11.0 32.0 11.0 37.0 20-21 21.560958631662686 22.0 11.0 32.0 11.0 37.0 22-23 20.969769291964994 21.0 11.0 32.0 10.5 37.0 24-25 20.876093874303898 21.0 11.0 31.0 10.5 37.0 26-27 20.646877486077962 21.0 11.0 31.0 10.5 37.0 28-29 20.36933174224344 21.0 11.0 29.5 11.0 37.0 30-31 20.27058472553699 21.0 11.0 29.5 11.0 37.0 32-33 20.488166268894194 21.0 11.0 29.0 11.0 36.0 34-35 20.87112171837709 21.0 11.0 31.0 11.0 37.0 36-37 20.603520286396183 21.0 11.0 29.0 11.0 37.0 38-39 19.931384248210023 16.0 11.0 27.0 11.0 36.0 40-41 20.20266507557677 16.0 11.0 27.0 11.0 36.0 42-43 20.469172633253777 21.0 11.0 29.0 11.0 36.5 44-45 19.976034208432775 16.0 11.0 27.0 11.0 36.0 46-47 20.2708830548926 16.0 11.0 27.0 11.0 36.0 48-49 20.625596658711217 21.0 11.0 29.0 11.0 36.0 50-51 20.897076372315034 21.0 11.0 31.0 11.0 36.0 52-53 20.9329753381066 21.0 11.0 31.0 11.0 36.0 54-55 20.787987271280826 21.0 11.0 31.0 11.0 36.0 56-57 20.193416865552905 20.0 11.0 28.5 11.0 36.0 58-59 20.193615751789977 16.0 11.0 29.0 11.0 36.0 60-61 20.559367541766107 21.0 11.0 29.0 11.0 36.0 62-63 20.065035799522676 19.5 11.0 27.5 11.0 36.0 64-65 20.36684566428003 20.0 11.0 29.0 11.0 36.0 66-67 20.48398965791567 19.5 11.0 30.5 11.0 36.0 68-69 20.113564041368335 19.0 11.0 28.0 11.0 35.5 70-71 19.705151153540175 19.0 11.0 27.0 11.0 35.5 72-73 20.261435958631665 19.0 11.0 28.5 11.0 36.0 74-75 20.203858392999205 19.0 11.0 27.5 11.0 35.0 76 19.793357199681783 19.0 11.0 27.0 11.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 3.1464052444663615 1101 2 1.327888480735563 1101 3 2.304739963929812 1101 4 0.5864969073409831 1101 5 2.1737570788993565 1101 6 5.850145645745709 1101 7 -2.793745580271711 1101 8 4.202581462383684 1101 9 -0.2841237230942326 1101 10-11 4.615905248292265 1101 12-13 0.555855361293311 1101 14-15 1.6493551727820126 1101 16-17 1.7668202031869562 1101 18-19 2.8690333597506843 1101 20-21 3.772990716292412 1101 22-23 7.344355349025879 1101 24-25 0.0701080395524869 1101 26-27 2.3097741771237494 1101 28-29 2.1091260377083323 1101 30-31 1.8054103691024856 1101 32-33 2.2944637451450163 1101 34-35 3.3409602503719817 1101 36-37 0.346600356080053 1101 38-39 1.793798274834522 1101 40-41 1.1797223838711304 1101 42-43 2.946111792680579 1101 44-45 -0.8496256893298835 1101 46-47 0.64554604465431 1101 48-49 0.3813332931038431 1101 50-51 -1.3071280912787877 1101 52-53 3.23418820844282 1101 54-55 2.884885947164779 1101 56-57 1.2121327999808287 1101 58-59 1.4959651774675535 1101 60-61 0.5220357769161872 1101 62-63 3.6277242413693322 1101 64-65 -0.2251127943494211 1101 66-67 -1.03015502633529 1101 68-69 -0.6031766110488803 1101 70-71 1.7001303046231264 1101 72-73 2.698620524688394 1101 74-75 0.21039721805300715 1101 76 4.365898948203267 1102 1 -2.6035947555336385 1102 2 0.5612218140688974 1102 3 0.6380732972631442 1102 4 2.669830240674319 1102 5 2.9737570788993573 1102 6 1.5501456457457081 1102 7 0.33958775306162536 1102 8 1.369248129050348 1102 9 -3.3007903897608983 1102 10-11 -0.5340947517077375 1102 12-13 0.38918869462663963 1102 14-15 -3.3506448272179874 1102 16-17 2.5751535365202898 1102 18-19 0.7773666930840157 1102 20-21 3.06465738295908 1102 22-23 1.6026886823592115 1102 24-25 -0.5382252937808474 1102 26-27 -2.2735591562095863 1102 28-29 1.3257927043749973 1102 30-31 0.6887437024358221 1102 32-33 -3.0222029215216466 1102 34-35 -0.042373082961351116 1102 36-37 0.04660035608005586 1102 38-39 -0.5062017251654787 1102 40-41 0.5547223838711304 1102 42-43 -1.1705548739860845 1102 44-45 -1.191292355996552 1102 46-47 -0.30445395534568576 1102 48-49 -0.3020000402294869 1102 50-51 -1.9654614246121191 1102 52-53 0.0591882084428228 1102 54-55 -0.8901140528352229 1102 56-57 0.912132799980828 1102 58-59 2.270965177467552 1102 60-61 0.9137024435828565 1102 62-63 -0.5806090919639999 1102 64-65 1.0498872056505775 1102 66-67 -1.34682169300196 1102 68-69 2.1134900556177882 1102 70-71 1.158463637956455 1102 72-73 -0.16804614197827306 1102 74-75 -0.5729361152803243 1102 76 1.7658989482032688 1103 1 0.1964052444663622 1103 2 0.9612218140688995 1103 3 -1.061926702736855 1103 4 -3.2801697593256804 1103 5 -0.726242921100642 1103 6 -1.849854354254294 1103 7 -3.1104122469383775 1103 8 -3.130751870949652 1103 9 -0.5507903897608983 1103 10-11 -0.3090947517077396 1103 12-13 -0.4108113053733575 1103 14-15 -1.7006448272179853 1103 16-17 -0.8248464634797088 1103 18-19 0.5523666930840143 1103 20-21 -1.68534261704092 1103 22-23 -0.5723113176407857 1103 24-25 -2.2132252937808445 1103 26-27 -2.3235591562095834 1103 28-29 -0.6742072956250027 1103 30-31 -1.9862562975641822 1103 32-33 -1.0972029215216494 1103 34-35 -0.4673730829613518 1103 36-37 0.5466003560800559 1103 38-39 -1.6312017251654787 1103 40-41 0.2297223838711311 1103 42-43 -2.2955548739860845 1103 44-45 0.4087076440034494 1103 46-47 -1.60445395534569 1103 48-49 1.2479999597705103 1103 50-51 -0.4904614246121213 1103 52-53 1.6591882084428242 1103 54-55 -2.990114052835221 1103 56-57 -1.56286720001917 1103 58-59 -1.5040348225324465 1103 60-61 0.53870244358286 1103 62-63 0.9193909080359965 1103 64-65 -2.850112794349421 1103 66-67 2.2281783069980428 1103 68-69 0.0384900556177854 1103 70-71 -4.391536362043542 1103 72-73 -1.0680461419782716 1103 74-75 -1.4729361152803229 1103 76 -2.5841010517967327 1104 1 0.22008945499267796 1104 2 1.5980639193320556 1104 3 1.5012311919999881 1104 4 -0.7749066014309456 1104 5 -0.3420323947848516 1104 6 -2.013012248991135 1104 7 -0.9446227732541672 1104 8 -0.32022555516017803 1104 9 4.712367504975944 1104 10-11 0.9948526167133132 1104 12-13 -0.4371270948470425 1104 14-15 3.644092014887274 1104 16-17 1.5685745891518685 1104 18-19 0.5655245878208568 1104 20-21 0.5238679092748662 1104 22-23 1.1040044718328978 1104 24-25 -0.9895410832545295 1104 26-27 -1.0498749456832712 1104 28-29 1.0863190201644706 1104 30-31 4.501901597172662 1104 32-33 3.3646391837415095 1104 34-35 1.344469022301805 1104 36-37 -0.7112943807620518 1104 38-39 -0.26935961990232116 1104 40-41 1.3323539628185017 1104 42-43 0.08470828390865037 1104 44-45 -0.47418709283865823 1104 46-47 0.21923025518062644 1104 48-49 0.21642101240209044 1104 50-51 0.8016438385457718 1104 52-53 1.6762934716007187 1104 54-55 -1.2677456317825921 1104 56-57 0.7647643789281986 1104 58-59 0.9275441248359719 1104 60-61 -0.5428765037855641 1104 62-63 -0.3740301445955794 1104 64-65 1.6643608898611078 1104 66-67 1.0294940964717263 1104 68-69 1.258226897723052 1104 70-71 -0.871799519938282 1104 72-73 -0.3575198261887991 1104 74-75 -1.2229361152803229 1104 76 0.32379368504537354 1105 1 1.0464052444663636 1105 2 0.1612218140688988 1105 3 -0.41192670273685295 1105 4 1.4698302406743196 1105 5 -2.3262429211006435 1105 6 -1.349854354254294 1105 7 1.6395877530616225 1105 8 1.0192481290503501 1105 9 4.149209610239101 1105 10-11 2.0659052482922604 1105 12-13 3.5891886946266425 1105 14-15 0.3993551727820126 1105 16-17 1.2001535365202898 1105 18-19 1.8023666930840143 1105 20-21 3.6396573829590793 1105 22-23 1.077688682359213 1105 24-25 1.1117747062191583 1105 26-27 4.0264408437904144 1105 28-29 1.625792704374998 1105 30-31 0.9137437024358199 1105 32-33 1.127797078478352 1105 34-35 -0.31737308296135325 1105 36-37 -2.0033996439199484 1105 38-39 0.19379827483452416 1105 40-41 0.5547223838711304 1105 42-43 -0.6205548739860873 1105 44-45 0.6587076440034494 1105 46-47 -1.5794539553456879 1105 48-49 -0.3020000402294869 1105 50-51 0.18453857538787943 1105 52-53 0.35918820844281996 1105 54-55 2.884885947164779 1105 56-57 0.16213279998083152 1105 58-59 1.49596517746755 1105 60-61 -2.5612975564171414 1105 62-63 2.344390908036001 1105 64-65 0.0748872056505796 1105 66-67 0.9031783069980399 1105 68-69 0.6884900556177911 1105 70-71 1.3834636379564564 1105 72-73 1.9819538580217255 1105 74-75 1.1770638847196757 1105 76 0.06589894820326592 1106 1 -1.503594755533637 1106 2 0.17372181406889808 1106 3 2.1130732972631456 1106 4 5.807330240674318 1106 5 4.973757078899357 1106 6 1.456457457074123E-4 1106 7 -0.44791224693837606 1106 8 1.5942481290503494 1106 9 3.4492096102391017 1106 10-11 3.509655248292262 1106 12-13 3.4016886946266425 1106 14-15 -0.06314482721798598 1106 16-17 3.0751535365202898 1106 18-19 3.4898666930840143 1106 20-21 3.1521573829590785 1106 22-23 2.577688682359213 1106 24-25 2.2555247062191555 1106 26-27 0.10144084379041374 1106 28-29 2.063292704374998 1106 30-31 2.957493702435819 1106 32-33 3.409047078478352 1106 34-35 0.7951269170386475 1106 36-37 1.4466003560800544 1106 38-39 2.9937982748345213 1106 40-41 0.7797223838711318 1106 42-43 2.823195126013914 1106 44-45 2.058707644003448 1106 46-47 2.8705460446543114 1106 48-49 1.7854999597705117 1106 50-51 3.209538575387878 1106 52-53 1.9591882084428214 1106 54-55 1.4098859471647778 1106 56-57 3.9933827999808287 1106 58-59 1.9834651774675507 1106 60-61 3.807452443582857 1106 62-63 0.9318909080359994 1106 64-65 2.7498872056505803 1106 66-67 0.8469283069980413 1106 68-69 0.7384900556177882 1106 70-71 -1.5165363620435421 1106 72-73 1.9944538580217284 1106 74-75 0.8708138847196771 1106 76 1.4783989482032673 1107 1 0.5464052444663636 1107 2 -3.2387781859311033 1107 3 -2.7119267027368537 1107 4 -1.5801697593256812 1107 5 -0.22624292110064204 1107 6 0.05014564574570812 1107 7 -0.16041224693837464 1107 8 -2.8307518709496513 1107 9 -2.5507903897608983 1107 10-11 -0.5590947517077396 1107 12-13 -3.010811305373359 1107 14-15 -2.7756448272179846 1107 16-17 -2.3998464634797116 1107 18-19 -4.322633306915986 1107 20-21 0.8646573829590771 1107 22-23 -0.9723113176407878 1107 24-25 -1.5132252937808452 1107 26-27 -2.823559156209587 1107 28-29 0.8257927043749973 1107 30-31 -1.71125629756418 1107 32-33 -3.4722029215216494 1107 34-35 -1.8173730829613532 1107 36-37 -0.07839964391994414 1107 38-39 -1.7562017251654787 1107 40-41 -3.1452776161288654 1107 42-43 -1.895554873986086 1107 44-45 -2.8662923559965527 1107 46-47 -1.35445395534569 1107 48-49 -3.5020000402294897 1107 50-51 -0.4654614246121227 1107 52-53 -2.3408117915571793 1107 54-55 0.20988594716477849 1107 56-57 -2.962867200019172 1107 58-59 -3.1290348225324465 1107 60-61 0.16370244358285646 1107 62-63 -0.03060909196399919 1107 64-65 0.22488720565058173 1107 66-67 -2.6468216930019572 1107 68-69 -1.0365099443822103 1107 70-71 1.6334636379564564 1107 72-73 -2.2430461419782723 1107 74-75 -1.3479361152803229 1107 76 1.5158989482032688 1108 1 -0.779910545007322 1108 2 -0.2966729227732081 1108 3 3.343336455157882 1108 4 0.5408828722532668 1108 5 1.5527044473204086 1108 6 2.2501456457457074 1108 7 -2.3656754048331123 1108 8 -0.6886466077917568 1108 9 -3.392895652918792 1108 10-11 -0.2156736990761594 1108 12-13 -0.1739692001102 1108 14-15 -1.9611711430074585 1108 16-17 0.3054166944150296 1108 18-19 -0.6186859384949308 1108 20-21 -1.897184722304079 1108 22-23 -1.922311317640787 1108 24-25 -0.20006739904400206 1108 26-27 -1.4182959983148464 1108 28-29 -0.8610494008881595 1108 30-31 2.2124279129621343 1108 32-33 -0.5564134478374356 1108 34-35 1.0286795486175926 1108 36-37 -0.31655753865678804 1108 38-39 -0.5062017251654787 1108 40-41 1.4902486996606044 1108 42-43 -0.23108118977555847 1108 44-45 -0.5531344612597096 1108 46-47 -0.5176118500825311 1108 48-49 0.19010522292840548 1108 50-51 -0.9088824772437007 1108 52-53 -2.5342328441887574 1108 54-55 0.10067542084899017 1108 56-57 1.1858170105071437 1108 58-59 -0.046140085690346666 1108 60-61 0.9044919172670696 1108 62-63 -0.847714355121898 1108 64-65 -2.1251127943494197 1108 66-67 0.13475725436646258 1108 68-69 -0.004930997013790517 1108 70-71 -1.4507468883593297 1108 72-73 -1.383835615662484 1108 74-75 0.9349586215617798 1108 76 2.1132673692558974 1109 1 0.6214052444663629 1109 2 -0.3887781859311019 1109 3 -1.9494267027368544 1109 4 -2.442669759325682 1109 5 -3.3387429211006427 1109 6 -0.8123543542542926 1109 7 -4.885412246938376 1109 8 -4.593251870949651 1109 9 -1.9882903897608983 1109 10-11 -1.7090947517077382 1109 12-13 -4.9108113053733575 1109 14-15 -1.438144827217986 1109 16-17 -2.0185964634797102 1109 18-19 1.0836166930840143 1109 20-21 -3.5353426170409215 1109 22-23 0.01518868235921289 1109 24-25 -3.5882252937808445 1109 26-27 -6.398559156209586 1109 28-29 -1.530457295625002 1109 30-31 -2.042506297564181 1109 32-33 -0.715952921521648 1109 34-35 -0.42362308296135254 1109 36-37 -1.5846496439199456 1109 38-39 -2.2562017251654787 1109 40-41 -0.9702776161288682 1109 42-43 -4.083054873986086 1109 44-45 0.15245764400344797 1109 46-47 -2.5044539553456886 1109 48-49 -2.6207500402294883 1109 50-51 -0.602961424612122 1109 52-53 -1.6345617915571786 1109 54-55 -1.0901140528352222 1109 56-57 -0.8816172000191713 1109 58-59 1.4209651774675507 1109 60-61 -1.3800475564171428 1109 62-63 1.0881409080359994 1109 64-65 -2.5938627943494197 1109 66-67 -0.30932169300195866 1109 68-69 -2.5115099443822118 1109 70-71 -3.954036362043542 1109 72-73 -2.1305461419782716 1109 74-75 -3.660436115280323 1109 76 -2.0216010517967327 1110 1 2.5321195301806476 1110 2 2.646936099783183 1110 3 2.1666447258345727 1110 4 0.7626873835314605 1110 5 3.759471364613642 1110 6 -0.03556863996857729 1110 7 1.5253020387759086 1110 8 -1.0307518709496506 1110 9 -1.7650761040466136 1110 10-11 2.165905248292262 1110 12-13 3.5534744089123578 1110 14-15 -0.3220733986465554 1110 16-17 -1.1927036063368526 1110 18-19 1.7666524073697296 1110 20-21 1.4468002401019362 1110 22-23 1.827688682359213 1110 24-25 2.5010604205048708 1110 26-27 3.940726558076129 1110 28-29 1.3043641329464286 1110 30-31 1.8458865595786733 1110 32-33 5.306368507049783 1110 34-35 1.7147697741815016 1110 36-37 2.6608860703657697 1110 38-39 0.9223697034059484 1110 40-41 1.8868652410139894 1110 42-43 2.4437308402996294 1110 44-45 1.5051362154320174 1110 46-47 3.174117473225742 1110 48-49 3.169428531199081 1110 50-51 3.9952528611021627 1110 52-53 0.5484739227285367 1110 54-55 0.4277430900219201 1110 56-57 0.926418514266544 1110 58-59 -0.9540348225324493 1110 60-61 1.8744167292971419 1110 62-63 1.1372480508931417 1110 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2.289793242640325 2117 3 4.095216154406003 2117 4 1.5484016692457452 2117 5 2.259471364613642 2117 6 4.107288502888565 2117 7 -1.1175551040812337 2117 8 3.4692481290503494 2117 9 5.449209610239102 2117 10-11 2.9873338197208312 2117 12-13 1.8749029803409272 2117 14-15 5.606498029924872 2117 16-17 3.771582107948859 2117 18-19 2.8737952645125837 2117 20-21 5.125371668673363 2117 22-23 2.791974396644928 2117 24-25 6.179631849076301 2117 26-27 2.3335837009332714 2117 28-29 6.947221275803567 2117 30-31 6.4530294167215345 2117 32-33 3.4135113641926367 2117 34-35 2.036198345610078 2117 36-37 2.732314641794339 2117 38-39 8.565226846263094 2117 40-41 7.5654366695854165 2117 42-43 3.8365879831567717 2117 44-45 5.255136215432021 2117 46-47 4.316974616082884 2117 48-49 7.633714245484796 2117 50-51 1.1738242896735933 2117 52-53 2.4056167798713908 2117 54-55 3.1420288043076354 2117 56-57 11.14070422855226 2117 58-59 6.510250891753266 2117 60-61 2.5172738721542878 2117 62-63 5.565819479464569 2117 64-65 2.4463157770791497 2117 66-67 3.788892592712326 2117 68-69 5.792061484189219 2117 70-71 2.3763207808136 2117 72-73 6.860525286593155 2117 74-75 4.8484924561482465 2117 76 2.451613233917552 2118 1 2.496405244466363 2118 2 -0.8887781859311019 2118 3 -0.011926702736854367 2118 4 1.1198302406743181 2118 5 -6.026242921100643 2118 6 -3.4998543542542926 2118 7 2.989587753061624 2118 8 -4.280751870949651 2118 9 5.449209610239102 2118 10-11 -8.084094751707738 2118 12-13 6.5891886946266425 2118 14-15 3.499355172782014 2118 16-17 -3.4248464634797102 2118 18-19 -2.6976333069159857 2118 20-21 -5.1603426170409215 2118 22-23 -2.047311317640787 2118 24-25 1.7867747062191555 2118 26-27 0.7264408437904137 2118 28-29 -2.249207295625002 2118 30-31 3.113743702435819 2118 32-33 -1.247202921521648 2118 34-35 -0.017373082961352537 2118 36-37 2.8216003560800544 2118 38-39 0.4937982748345213 2118 40-41 2.529722383871132 2118 42-43 -1.645554873986086 2118 44-45 0.05870764400344797 2118 46-47 3.2455460446543114 2118 48-49 1.0979999597705117 2118 50-51 -0.415461424612122 2118 52-53 0.3341882084428214 2118 54-55 0.9098859471647778 2118 56-57 1.2121327999808287 2118 58-59 4.795965177467551 2118 60-61 0.08870244358285717 2118 62-63 -5.880609091964001 2118 64-65 0.6248872056505803 2118 66-67 3.6281783069980413 2118 68-69 -3.7615099443822118 2118 70-71 7.108463637956458 2118 72-73 3.1819538580217284 2118 74-75 5.027063884719677 2118 76 -0.33410105179673266 2119 1 -0.9202614222003049 2119 2 5.527888480735566 2119 3 2.2380732972631456 2119 4 4.45316357400765 2119 5 2.9737570788993573 2119 6 6.916812312412375 2119 7 2.739587753061624 2119 8 8.46924812905035 2119 9 1.7825429435724338 2119 10-11 -1.750761418374406 2119 12-13 -0.4108113053733575 2119 14-15 -0.5839781605513181 2119 16-17 1.2001535365202898 2119 18-19 0.8023666930840143 2119 20-21 -1.1603426170409215 2119 22-23 -4.088977984307455 2119 24-25 -3.1298919604475124 2119 26-27 2.9764408437904137 2119 28-29 -6.624207295625002 2119 30-31 -9.594589630897515 2119 32-33 1.627797078478352 2119 34-35 1.4409602503719832 2119 36-37 3.1132670227467187 2119 38-39 -3.5062017251654787 2119 40-41 -1.053610949462204 2119 42-43 1.6461117926805784 2119 44-45 -0.816292355996552 2119 46-47 -3.2544539553456886 2119 48-49 -0.9853333735628205 2119 50-51 -0.540461424612122 2119 52-53 -2.1658117915571786 2119 54-55 -1.0484473861685544 2119 56-57 -6.454533866685839 2119 58-59 -2.037368155865785 2119 60-61 -0.24463088975047498 2119 62-63 -4.838942425297333 2119 64-65 4.37488720565058 2119 66-67 -0.8301550263352908 2119 68-69 -0.803176611048876 2119 70-71 -4.099869695376876 2119 72-73 3.265287191355064 2119 74-75 -3.722936115280323 2119 76 -5.500767718463399 >>END_MODULE >>Per sequence quality scores fail #Quality Count 12 10.0 13 74.0 14 207.0 15 376.0 16 441.0 17 600.0 18 649.0 19 597.0 20 456.0 21 367.0 22 236.0 23 163.0 24 105.0 25 83.0 26 57.0 27 54.0 28 45.0 29 42.0 30 29.0 31 25.0 32 27.0 33 33.0 34 56.0 35 64.0 36 109.0 37 123.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.730692276910766 25.990396158463387 10.124049619847938 37.15486194477791 2 5.310262529832936 24.801113762927603 25.69610182975338 44.19252187748608 3 5.946698488464598 26.292760540970566 37.5497215592681 30.21081941129674 4 3.8385043754972155 46.47971360381862 20.54494828957836 29.13683373110581 5 4.435163086714399 31.722354813046937 37.03261734287987 26.809864757358792 6 6.046141607000796 32.517899761336515 12.529832935560858 48.90612569610183 7 5.4494828957836114 49.62211614956245 10.202863961813842 34.7255369928401 8 4.932378679395385 37.370723945902945 11.933174224343675 45.76372315035799 9 8.452665075576771 30.38981702466189 12.012728719172634 49.1447891805887 10-11 6.7919649960222745 32.38862370723946 23.130469371519492 37.68894192521878 12-13 7.2295147175815435 28.21201272871917 22.80230708035004 41.75616547334924 14-15 11.445902943516309 31.831742243436757 17.73070803500398 38.99164677804296 16-17 15.662291169451073 31.801909307875896 17.203659506762133 35.3321400159109 18-19 11.684566428003182 29.73349244232299 16.994828957836116 41.58711217183771 20-21 10.819411296738265 28.868337311058074 18.486475735879075 41.82577565632458 22-23 17.541766109785204 29.952267303102627 14.866746221161495 37.63922036595068 24-25 13.345266507557676 34.25815433571997 14.976133651551313 37.42044550517104 26-27 11.41607000795545 36.22712808273668 18.556085918854414 33.80071599045346 28-29 14.071201272871917 30.519093078758953 23.339299920445505 32.07040572792363 30-31 14.130867143993637 29.534606205250597 24.701670644391406 31.632856006364356 32-33 10.003977724741448 36.097852028639615 21.499602227525855 32.39856801909308 34-35 10.192919649960224 35.61058074781226 19.8587907716786 34.33770883054893 36-37 12.002784407319012 29.594272076372313 22.981304693715195 35.42163882259348 38-39 12.49005568814638 25.626491646778042 26.680588703261733 35.20286396181384 40-41 10.232696897374701 28.28162291169451 24.333731105807477 37.151949085123306 42-43 6.205250596658711 25.208830548926013 28.400954653937948 40.184964200477324 44-45 7.567621320604615 26.402147971360385 30.787589498806682 35.24264120922832 46-47 7.955449482895784 31.404136833731105 26.02426412092283 34.616149562450275 48-49 7.746618933969769 34.834924423229914 20.425616547334926 36.9928400954654 50-51 7.766507557677009 33.40294351630867 18.973747016706444 39.856801909307876 52-53 6.573190135242641 32.64717581543357 21.002386634844868 39.777247414478914 54-55 7.289180588703262 31.483691328560067 19.401352426412092 41.82577565632458 56-57 11.097852028639618 32.159904534606206 18.307478122513924 38.43476531424025 58-59 9.685759745425617 30.92680986475736 18.546141607000795 40.841288782816235 60-61 7.577565632458234 28.96778042959427 18.695306284805092 44.7593476531424 62-63 10.242641209228323 29.643993635640413 18.536197295147176 41.57716785998409 64-65 10.073587907716785 29.92243436754177 15.04574383452665 44.9582338902148 66-67 8.552108194112968 36.34645982498011 13.056881463802705 42.04455051710422 68-69 11.117740652346859 35.99840891010342 12.619331742243437 40.264518695306286 70-71 13.484486873508352 37.370723945902945 11.75417661097852 37.390612569610184 72-73 9.337708830548925 35.68019093078759 12.072394590294351 42.90970564836913 74-75 8.542163882259347 37.33094669848847 12.589498806682577 41.53739061256961 76 6.8615751789976125 32.537788385043754 12.907716785998408 47.692919649960224 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 1.0 9 0.5 10 0.0 11 0.0 12 0.0 13 1.0 14 2.0 15 1.5 16 1.0 17 1.0 18 2.5 19 4.0 20 5.0 21 6.0 22 5.5 23 8.0 24 10.5 25 10.0 26 14.5 27 18.0 28 22.5 29 28.0 30 37.0 31 42.0 32 49.5 33 61.0 34 65.0 35 85.0 36 119.5 37 138.0 38 136.0 39 150.0 40 178.0 41 207.0 42 224.0 43 238.0 44 262.5 45 291.0 46 309.0 47 301.5 48 300.0 49 302.0 50 298.0 51 310.5 52 295.5 53 263.5 54 259.0 55 228.0 56 189.5 57 160.5 58 139.0 59 135.0 60 122.5 61 108.5 62 103.0 63 82.5 64 59.5 65 48.0 66 38.0 67 37.0 68 29.5 69 20.5 70 13.5 71 8.0 72 10.5 73 10.5 74 6.5 75 5.0 76 6.5 77 5.0 78 1.5 79 1.0 80 2.0 81 2.5 82 1.0 83 0.0 84 0.5 85 0.5 86 1.0 87 2.0 88 1.5 89 1.0 90 0.5 91 1.0 92 2.0 93 2.5 94 2.0 95 1.0 96 1.0 97 1.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5966587112171837 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 5028.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.25536992840095 #Duplication Level Percentage of deduplicated Percentage of total 1 98.03680981595092 95.34606205250597 2 1.492842535787321 2.903739061256961 3 0.26584867075664625 0.7756563245823389 4 0.10224948875255625 0.3977724741447892 5 0.0408997955010225 0.1988862370723946 6 0.0408997955010225 0.23866348448687352 7 0.02044989775051125 0.13922036595067622 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTG 7 0.13922036595067622 No Hit CCTTTGAAATCTGTTACCAGGTGATAGGTTACACACTGTCTCTTATACAC 6 0.11933174224343676 No Hit TATGTAAATTATATAAATCTGAGATAAAATAAAGGCATTTCATAACCTAA 6 0.11933174224343676 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.059665871121718374 0.0 2 0.0 0.0 0.0 0.059665871121718374 0.0 3 0.0 0.0 0.0 0.059665871121718374 0.0 4 0.0 0.0 0.0 0.059665871121718374 0.0 5 0.0 0.0 0.0 0.059665871121718374 0.0 6 0.0 0.0 0.0 0.059665871121718374 0.0 7 0.0 0.0 0.0 0.059665871121718374 0.0 8 0.0 0.0 0.0 0.059665871121718374 0.0 9 0.0 0.0 0.0 0.07955449482895784 0.0 10 0.0 0.0 0.0 0.07955449482895784 0.0 11 0.0 0.0 0.0 0.11933174224343675 0.0 12 0.0 0.0 0.0 0.19888623707239458 0.0 13 0.0 0.0 0.0 0.33810660302307083 0.0 14 0.0 0.0 0.0 0.33810660302307083 0.0 15 0.0 0.0 0.0 0.33810660302307083 0.0 16 0.0 0.0 0.0 0.33810660302307083 0.0 17 0.0 0.0 0.0 0.33810660302307083 0.0 18 0.0 0.0 0.0 0.5568814638027049 0.0 19 0.0 0.0 0.0 0.7159904534606205 0.0 20 0.0 0.0 0.0 0.7955449482895783 0.0 21 0.0 0.0 0.0 0.8552108194112967 0.0 22 0.0 0.0 0.0 0.9148766905330151 0.0 23 0.0 0.0 0.0 0.954653937947494 0.0 24 0.0 0.0 0.0 0.954653937947494 0.0 25 0.0 0.0 0.0 0.994431185361973 0.0 26 0.0 0.0 0.0 0.994431185361973 0.0 27 0.0 0.0 0.0 0.994431185361973 0.0 28 0.0 0.0 0.0 1.0342084327764518 0.0 29 0.0 0.0 0.0 1.0540970564836913 0.0 30 0.0 0.0 0.0 1.0739856801909309 0.0 31 0.0 0.0 0.0 1.1535401750198886 0.0 32 0.0 0.0 0.0 1.213206046141607 0.0 33 0.0 0.0 0.0 1.2330946698488465 0.0 34 0.0 0.0 0.0 1.3126491646778042 0.0 35 0.0 0.0 0.0 1.3922036595067622 0.0 36 0.0 0.0 0.0 1.7700875099443119 0.0 37 0.0 0.0 0.0 1.8894192521877486 0.0 38 0.0 0.0 0.0 2.167859984089101 0.0 39 0.0 0.0 0.0 2.4065234685759744 0.0 40 0.0 0.0 0.0 2.58552108194113 0.0 41 0.0 0.0 0.0 2.863961813842482 0.0 42 0.0 0.0 0.0 3.042959427207637 0.0 43 0.0 0.0 0.0 3.2617342879872715 0.0 44 0.0 0.0 0.0 3.460620525059666 0.0 45 0.0 0.0 0.0 3.5600636435958632 0.0 46 0.0 0.0 0.0 3.639618138424821 0.0 47 0.0 0.0 0.0 3.9379474940334127 0.0 48 0.0 0.0 0.0 4.176610978520286 0.0 49 0.0 0.0 0.0 4.315831344470962 0.0 50 0.0 0.0 0.0 4.41527446300716 0.0 51 0.0 0.0 0.0 4.534606205250597 0.0 52 0.0 0.0 0.0 4.852824184566428 0.0 53 0.0 0.0 0.0 5.011933174224343 0.0 54 0.0 0.0 0.0 5.17104216388226 0.0 55 0.0 0.0 0.0 5.469371519490851 0.0 56 0.0 0.0 0.0 5.628480509148767 0.0 57 0.0 0.0 0.0 5.708035003977725 0.0 58 0.0 0.0 0.0 5.827366746221162 0.0 59 0.0 0.0 0.0 5.946698488464598 0.0 60 0.0 0.0 0.0 6.046141607000796 0.0 61 0.0 0.0 0.0 6.105807478122514 0.0 62 0.0 0.0 0.0 6.125696101829753 0.0 63 0.0 0.0 0.0 6.225139220365951 0.0 64 0.0 0.0 0.0 6.304693715194909 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCTGCC 50 0.0065552024 28.000002 13 >>END_MODULE