FastQCFastQC Report
Wed 25 May 2016
SRR1780576_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780576_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5028
Sequences flagged as poor quality0
Sequence length76
%GC43

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT143428.520286396181383No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT62812.49005568814638RNA PCR Primer, Index 24 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG1903.7788385043754973No Hit
CTTTACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC1282.5457438345266508RNA PCR Primer, Index 24 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC1142.267303102625298No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATATCGTATGCCGT811.6109785202863964No Hit
CTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC711.4120922832140015No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGT551.0938743038981702No Hit
TATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCT531.0540970564836913RNA PCR Primer, Index 24 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCGT521.0342084327764518No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC320.6364359586316627No Hit
ACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTTCT310.6165473349244232RNA PCR Primer, Index 24 (96% over 25bp)
CTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG270.5369928400954653No Hit
CTTATACACATCTCCGAGCCCACGCGACGTGATAGCATCTCGTATGCCGT230.4574383452665076No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCGTCTT210.41766109785202865No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGTCTT170.3381066030230708No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATATCGTATGCCG170.3381066030230708No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTAGTATGCCGT160.31821797931583135No Hit
CTTATACACATCTCCGAGCCCACGAGCCGTGATAGCATCTCGTATGCCGT160.31821797931583135No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATATCGTATGCCGTCTT150.29832935560859186No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC150.29832935560859186No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCG150.29832935560859186No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATCGCATCTCGTATGCCGT140.27844073190135243No Hit
CTTATACACATCTCCGAGCACACGAGACGTGATAGCATCTCGTATGCCGT140.27844073190135243No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCC140.27844073190135243No Hit
ATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTTCTGCTT130.25855210819411295RNA PCR Primer, Index 34 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGTGACGTGATAGCATCTCGTATGCCGT130.25855210819411295No Hit
CTTAACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC110.21877486077963404RNA PCR Primer, Index 24 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGC100.1988862370723946No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCAGT100.1988862370723946No Hit
TTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC100.1988862370723946No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGATAGCATCTCGTATGCCGT100.1988862370723946No Hit
CTTATTACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG100.1988862370723946No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAGCTCGTATGCCGT100.1988862370723946No Hit
CTTATACACATATCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT100.1988862370723946No Hit
CTTATACACCTCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT90.17899761336515513No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCCTCTCGTATGCCGT90.17899761336515513No Hit
CTTATAACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG90.17899761336515513No Hit
ATTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT90.17899761336515513No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCCCGTATGCCGT90.17899761336515513No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGACGT80.15910898965791567No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATATCGTATGCC80.15910898965791567No Hit
CTTATACACATCTCCGAGCCCAAGAGACGTGATAGCATCTCGTATGCCGT80.15910898965791567No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGCTAGCATCTCGTATGCCGT80.15910898965791567No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAAATCGTATGCCGT80.15910898965791567No Hit
CTTATACACATCTCCGAGCCAACGAGACGTGATAGCATCTCGTATGCCGT70.13922036595067622No Hit
CTTATACACATCTACGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT70.13922036595067622No Hit
CGTCAGATGTGTATAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA70.13922036595067622No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCTTCTCGTATGCCGT60.11933174224343676No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCACGTATGCCGT60.11933174224343676No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCC60.11933174224343676No Hit
CTTTACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCGTC60.11933174224343676No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCCT60.11933174224343676RNA PCR Primer, Index 24 (95% over 21bp)
CATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTTCTGCT60.11933174224343676RNA PCR Primer, Index 24 (96% over 28bp)
CTTATCACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC60.11933174224343676No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTCTGCCGTCTT60.11933174224343676No Hit
TCTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT60.11933174224343676No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCCTCTCGTATGCCGTCTT60.11933174224343676No Hit
CTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTTCTGCTTGA60.11933174224343676RNA PCR Primer, Index 24 (96% over 30bp)
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCG60.11933174224343676No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGTA150.002097236270.038
TAGCATA150.002097236270.033
AGCATAT150.002097236270.034
ATATCGT150.002097236270.037
CATATCG150.002097236270.036
GCATATC150.002097236270.035
CTTATAC3100.060.9677431
TTATACA3150.060.02
TATACAC3250.057.076923
GAGAAGT200.00655606352.524
AGAAGTG200.00655606352.525
GAAGTGA200.00655606352.526
ACGAGAA200.00655606352.522
AGTGATA200.00655606352.528
GCTTGAC200.00655606352.556
AAGTGAT200.00655606352.527
CGAGAAG200.00655606352.523
CTTGACA200.00655606352.557
CACGAGA4500.042.021
CTGCTTG4250.042.054
Produced by FastQC (version 0.11.5)