##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780576_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5028 Sequences flagged as poor quality 0 Sequence length 76 %GC 43 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.26650755767701 34.0 34.0 34.0 27.0 34.0 2 32.72891805887033 34.0 34.0 34.0 31.0 34.0 3 33.144192521877486 34.0 34.0 34.0 32.0 34.0 4 33.42342879872713 34.0 34.0 34.0 33.0 34.0 5 33.3623707239459 34.0 34.0 34.0 34.0 34.0 6 37.15930787589499 38.0 37.0 38.0 36.0 38.0 7 36.66010342084328 38.0 37.0 38.0 34.0 38.0 8 36.69172633253779 38.0 37.0 38.0 34.0 38.0 9 36.41865552903739 38.0 37.0 38.0 34.0 38.0 10-11 36.01441925218775 38.0 36.5 38.0 32.5 38.0 12-13 35.822792362768496 38.0 36.0 38.0 31.5 38.0 14-15 35.7917661097852 38.0 36.0 38.0 31.5 38.0 16-17 35.76312649164677 38.0 36.0 38.0 31.0 38.0 18-19 35.73448687350835 38.0 35.5 38.0 31.0 38.0 20-21 35.51163484486874 38.0 36.0 38.0 31.0 38.0 22-23 35.699184566428 37.5 35.5 38.0 32.5 38.0 24-25 34.121221161495626 37.0 32.5 38.0 24.5 38.0 26-27 34.23836515513126 37.0 34.0 38.0 25.0 38.0 28-29 34.27217581543357 37.0 34.0 38.0 25.5 38.0 30-31 33.674124900556876 37.0 34.0 38.0 22.5 38.0 32-33 34.77973349244232 37.0 34.5 38.0 28.5 38.0 34-35 33.9546539379475 37.0 34.0 38.0 24.0 38.0 36-37 33.94391408114558 37.0 34.0 38.0 24.0 38.0 38-39 29.82627287191726 34.0 26.0 37.0 11.0 38.0 40-41 31.647474144789182 35.0 30.5 37.0 16.0 38.0 42-43 34.0659307875895 37.0 34.0 38.0 25.5 38.0 44-45 34.623110580747806 37.0 34.0 38.0 27.0 38.0 46-47 34.56523468575975 37.0 34.0 38.0 26.0 38.0 48-49 34.01471758154336 37.0 34.0 38.0 24.5 38.0 50-51 33.86674622116149 37.0 33.5 38.0 24.0 38.0 52-53 33.390811455847256 37.0 34.0 38.0 23.0 38.0 54-55 33.982895783611774 37.0 34.0 38.0 25.0 38.0 56-57 34.07587509944312 37.0 34.0 38.0 24.0 38.0 58-59 33.58522275258552 37.0 34.0 38.0 24.0 38.0 60-61 33.27138027048528 37.0 33.0 38.0 22.5 38.0 62-63 34.24940334128878 37.0 34.0 38.0 25.0 38.0 64-65 34.67243436754177 37.0 34.5 38.0 26.5 38.0 66-67 32.45674224343675 36.5 31.0 38.0 21.0 38.0 68-69 29.21698488464598 34.0 23.0 37.0 11.0 38.0 70-71 25.756662688941923 27.5 16.0 35.5 10.5 37.5 72-73 21.23249801113763 22.0 11.0 29.5 10.5 35.5 74-75 20.444908512330947 21.0 11.0 27.0 11.0 34.5 76 20.78480509148767 21.0 11.0 28.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -1.1032105497485176 1101 2 -0.45504482652874145 1101 3 -0.8654851148484326 1101 4 0.6183035338955207 1101 5 0.49599694352644974 1101 6 -1.6362992404082277 1101 7 0.5343980082446294 1101 8 1.093520351848845 1101 9 0.7673681608952521 1101 10-11 0.18858802017925314 1101 12-13 1.1831625344152314 1101 14-15 1.5635442047121302 1101 16-17 1.4179977683261455 1101 18-19 1.58388474258048 1101 20-21 -0.595786786164183 1101 22-23 0.7782178552086023 1101 24-25 -0.5815384647390829 1101 26-27 -0.7439271414403663 1101 28-29 0.7452603899419756 1101 30-31 2.136367686352216 1101 32-33 2.022781921905903 1101 34-35 1.792043157374522 1101 36-37 1.8793511545627197 1101 38-39 1.3698519940181164 1101 40-41 3.095931288878326 1101 42-43 2.3036247768427955 1101 44-45 1.898539064584753 1101 46-47 2.7608569642628424 1101 48-49 0.3696766806949725 1101 50-51 -0.6530920173646066 1101 52-53 -0.7597550032560179 1101 54-55 -0.13548617142201635 1101 56-57 0.7372195587687571 1101 58-59 -1.1121854109820468 1101 60-61 -0.5027217540978057 1101 62-63 -0.66723384789357 1101 64-65 -0.41572998022600416 1101 66-67 -1.2735718977047874 1101 68-69 1.0968134436224908 1101 70-71 2.0866715988143163 1101 72-73 -1.3385354041131805 1101 74-75 0.7787095369646195 1101 76 -0.6351559077993869 1102 1 0.11345611691815094 1102 2 0.8616218401379285 1102 3 0.9845148851515688 1102 4 0.48497020056218787 1102 5 0.34599694352645116 1102 6 0.4637007595917737 1102 7 0.6677313415779622 1102 8 0.9768536851821779 1102 9 0.5507014942285835 1102 10-11 1.1885880201792531 1102 12-13 1.5248292010819 1102 14-15 0.6302108713787931 1102 16-17 1.1846644349928113 1102 18-19 0.5838847425804801 1102 20-21 1.1792132138358227 1102 22-23 -0.0301154781247277 1102 24-25 -6.873205131405747 1102 26-27 -0.635593808107032 1102 28-29 1.286927056608647 1102 30-31 1.9613676863522187 1102 32-33 -0.11055141142743707 1102 34-35 0.9503764907078605 1102 36-37 -2.9789821787706146 1102 38-39 0.4115186606847807 1102 40-41 1.8625979555449916 1102 42-43 2.0452914435094556 1102 44-45 2.6318723979180945 1102 46-47 2.7775236309295153 1102 48-49 -3.3803233193050346 1102 50-51 0.16357464930206334 1102 52-53 0.7985783300773122 1102 54-55 1.3728471619113094 1102 56-57 2.49555289210209 1102 58-59 0.8294812556846267 1102 60-61 -7.377721754097802 1102 62-63 -2.8755671812269057 1102 64-65 0.9426033531073372 1102 66-67 2.0180947689618804 1102 68-69 -0.3031865563775078 1102 70-71 1.4616715988143127 1102 72-73 -1.9135354041131798 1102 74-75 0.49537620363128454 1102 76 5.031510758867277 1103 1 0.5634561169181467 1103 2 0.9116218401379257 1103 3 0.8845148851515674 1103 4 0.03497020056219213 1103 5 0.24599694352644974 1103 6 0.563700759591768 1103 7 0.5177313415779565 1103 8 0.8768536851821835 1103 9 0.8007014942285835 1103 10-11 -0.08641197982075255 1103 12-13 -2.2501707989180986 1103 14-15 0.15521087137879874 1103 16-17 0.6096644349928084 1103 18-19 1.2588847425804843 1103 20-21 0.42921321383582267 1103 22-23 0.8698845218752709 1103 24-25 -3.3982051314057458 1103 26-27 0.16440619189296513 1103 28-29 0.0869270566086442 1103 30-31 1.061367686352213 1103 32-33 0.9894485885725715 1103 34-35 0.5753764907078605 1103 36-37 -2.953982178770609 1103 38-39 1.73651866068478 1103 40-41 -0.2374020444550098 1103 42-43 1.8702914435094584 1103 44-45 1.7318723979180888 1103 46-47 1.4525236309295124 1103 48-49 -5.105323319305036 1103 50-51 0.23857464930205907 1103 52-53 0.9735783300773164 1103 54-55 1.097847161911318 1103 56-57 0.9455528921020857 1103 58-59 0.6044812556846182 1103 60-61 -4.727721754097804 1103 62-63 -1.4005671812269043 1103 64-65 1.1926033531073372 1103 66-67 -2.1069052310381196 1103 68-69 -0.5281865563775092 1103 70-71 2.661671598814312 1103 72-73 -3.763535404113181 1103 74-75 1.1453762036312831 1103 76 -0.3684892411327212 1104 1 0.9739824327076221 1104 2 0.35899026119055577 1104 3 1.1345148851515674 1104 4 -0.20976664154306945 1104 5 -0.13558200384196795 1104 6 0.39527970696018855 1104 7 1.0466787099990142 1104 8 1.2163273693927081 1104 9 1.3954383363338465 1104 10-11 -0.9587804008733798 1104 12-13 1.3919344642397888 1104 14-15 0.7144213976945863 1104 16-17 -0.09428293342824645 1104 18-19 0.9680952688962776 1104 20-21 0.6831605822568747 1104 22-23 0.9290950481910585 1104 24-25 1.0991632896468815 1104 26-27 3.1315114550508554 1104 28-29 3.290874425029699 1104 30-31 0.3732097916153734 1104 32-33 -0.09476193774322894 1104 34-35 1.3464291222868 1104 36-37 3.2407546633346485 1104 38-39 2.3207291870005697 1104 40-41 2.7099663765976203 1104 42-43 0.6229230224568312 1104 44-45 1.4423987137075684 1104 46-47 2.5748920519821397 1104 48-49 0.364413522800227 1104 50-51 2.43462728088101 1104 52-53 2.336736224814153 1104 54-55 1.0820576882271027 1104 56-57 2.4705528921020914 1104 58-59 -2.0507819022101152 1104 60-61 0.1446466669548272 1104 62-63 2.5520643977204642 1104 64-65 2.9807612478441783 1104 66-67 2.129936874225038 1104 68-69 6.775760812043547 1104 70-71 6.149829493551156 1104 72-73 -1.1437985620079196 1104 74-75 -0.8177816911055587 1104 76 0.5262476009725425 1105 1 0.6134561169181509 1105 2 1.4116218401379257 1105 3 1.1345148851515674 1105 4 0.48497020056218787 1105 5 -0.05400305647354742 1105 6 0.26370075959177086 1105 7 0.9677313415779594 1105 8 -2.523146314817822 1105 9 0.9507014942285892 1105 10-11 1.2135880201792446 1105 12-13 -0.9001707989181043 1105 14-15 0.6302108713787931 1105 16-17 -0.31533556500718873 1105 18-19 1.20888474258048 1105 20-21 1.0542132138358227 1105 22-23 -0.7801154781247277 1105 24-25 1.3517948685942542 1105 26-27 0.739406191892968 1105 28-29 1.7369270566086499 1105 30-31 0.8613676863522173 1105 32-33 1.389448588572563 1105 34-35 -2.4246235092921467 1105 36-37 0.04601782122939113 1105 38-39 -0.08848133931521929 1105 40-41 2.6875979555449874 1105 42-43 -0.8297085564905373 1105 44-45 1.531872397918086 1105 46-47 2.0525236309295067 1105 48-49 0.9446766806949682 1105 50-51 2.863574649302059 1105 52-53 1.4735783300773164 1105 54-55 1.5478471619113208 1105 56-57 2.0955528921020914 1105 58-59 2.629481255684624 1105 60-61 2.7972782459021985 1105 62-63 0.29943281877309147 1105 64-65 2.0426033531073386 1105 66-67 1.6180947689618748 1105 68-69 2.896813443622495 1105 70-71 -0.2633284011856851 1105 72-73 -2.338535404113177 1105 74-75 -1.2296237963687169 1105 76 -0.8184892411327205 1106 1 -0.5490438830818505 1106 2 0.1616218401379257 1106 3 1.1345148851515674 1106 4 0.6849702005621907 1106 5 0.49599694352644974 1106 6 0.26370075959177086 1106 7 -6.719768658422041 1106 8 -1.1356463148178193 1106 9 -4.624298505771414 1106 10-11 -0.8864119798207497 1106 12-13 2.0498292010818986 1106 14-15 -2.2822891286212013 1106 16-17 -0.20283556500719158 1106 18-19 1.127634742580483 1106 20-21 1.5729632138358198 1106 22-23 1.4948845218752709 1106 24-25 0.2142948685942514 1106 26-27 2.320656191892965 1106 28-29 2.024427056608644 1106 30-31 1.2613676863522159 1106 32-33 0.7456985885725658 1106 34-35 3.7066264907078548 1106 36-37 2.3460178212293883 1106 38-39 -1.6759813393152179 1106 40-41 1.2938479555449902 1106 42-43 1.4140414435094613 1106 44-45 2.606872397918089 1106 46-47 2.2837736309295096 1106 48-49 1.5009266806949668 1106 50-51 0.45107464930206476 1106 52-53 2.1360783300773107 1106 54-55 -0.22715283808868492 1106 56-57 -0.7169471078979086 1106 58-59 -0.35176874431537897 1106 60-61 0.9160282459021971 1106 62-63 1.7806828187730943 1106 64-65 -2.001146646892664 1106 66-67 1.7680947689618804 1106 68-69 5.009313443622492 1106 70-71 -0.8320784011856865 1106 72-73 1.1177145958868202 1106 74-75 0.32037620363128383 1106 76 -0.3684892411327212 1107 1 1.113456116918151 1107 2 -0.7883781598620736 1107 3 -0.01548511484843118 1107 4 -0.01502979943781213 1107 5 0.34599694352645116 1107 6 -0.23629924040822914 1107 7 1.1677313415779622 1107 8 -0.27314631481782214 1107 9 0.35070149422858776 1107 10-11 0.7635880201792489 1107 12-13 1.1498292010819 1107 14-15 0.9302108713787973 1107 16-17 2.009664434992807 1107 18-19 1.8088847425804815 1107 20-21 1.8042132138358227 1107 22-23 -0.0051154781247291226 1107 24-25 1.4017948685942514 1107 26-27 0.26440619189295944 1107 28-29 0.8619270566086499 1107 30-31 1.4613676863522187 1107 32-33 1.0394485885725686 1107 34-35 0.8503764907078519 1107 36-37 1.8960178212293854 1107 38-39 -0.7134813393152193 1107 40-41 0.18759795554499092 1107 42-43 1.8202914435094613 1107 44-45 1.656872397918086 1107 46-47 1.6275236309295096 1107 48-49 1.9196766806949697 1107 50-51 1.6385746493020648 1107 52-53 2.623578330077308 1107 54-55 0.7478471619113094 1107 56-57 1.0955528921020914 1107 58-59 1.8544812556846182 1107 60-61 1.9722782459022028 1107 62-63 1.1994328187730972 1107 64-65 0.6676033531073386 1107 66-67 -0.8069052310381224 1107 68-69 2.321813443622492 1107 70-71 1.8616715988143149 1107 72-73 1.0614645958868216 1107 74-75 -1.6796237963687162 1107 76 -0.2184892411327226 1108 1 1.026614011654992 1108 2 1.253727103295823 1108 3 0.7660938325199851 1108 4 0.6849702005621907 1108 5 0.49599694352644974 1108 6 0.18475339117071599 1108 7 0.3098366047358567 1108 8 -0.9941989463967644 1108 9 0.8691225468601687 1108 10-11 1.5675353886003052 1108 12-13 1.6024607800292685 1108 14-15 0.10915823979985362 1108 16-17 -1.7258618807966641 1108 18-19 0.3365163215278528 1108 20-21 1.7094763717305597 1108 22-23 0.11330557450684609 1108 24-25 1.2570580264889912 1108 26-27 -0.657962229159665 1108 28-29 0.317190214503384 1108 30-31 1.6889992652995858 1108 32-33 1.2999749043620383 1108 34-35 -0.23251824613424787 1108 36-37 3.1618072949136007 1108 38-39 2.715466029105837 1108 40-41 1.9731242713344628 1108 42-43 -2.3244453985958025 1108 44-45 1.0213460821286162 1108 46-47 0.33804994671898925 1108 48-49 1.0749398385897067 1108 50-51 0.3030483335125922 1108 52-53 1.7577888563930983 1108 54-55 -0.6284686275623699 1108 56-57 0.8652897342073516 1108 58-59 2.554481255684621 1108 60-61 2.197278245902197 1108 62-63 1.5783801871941492 1108 64-65 1.7439191425810208 1108 66-67 2.656252663698723 1108 68-69 2.723129233096177 1108 70-71 -3.5080652432909503 1108 72-73 -1.5648511935868648 1108 74-75 -2.2388343226845038 1108 76 -1.210594504290615 1109 1 -1.4240438830818505 1109 2 -0.08837815986207431 1109 3 1.0720148851515674 1109 4 0.4974702005621907 1109 5 0.43349694352644974 1109 6 -0.17379924040822914 1109 7 -1.5947686584220406 1109 8 0.1143536851821807 1109 9 0.31320149422858634 1109 10-11 -0.4489119798207497 1109 12-13 0.7685792010818986 1109 14-15 0.8739608713787987 1109 16-17 1.5159144349928084 1109 18-19 1.690134742580483 1109 20-21 1.9479632138358198 1109 22-23 1.0573845218752709 1109 24-25 -1.6294551314057486 1109 26-27 -0.2730938081070349 1109 28-29 0.5244270566086442 1109 30-31 0.48011768635221586 1109 32-33 1.2456985885725658 1109 34-35 0.33162649070785477 1109 36-37 2.1585178212293883 1109 38-39 2.730268660684782 1109 40-41 3.01259795554499 1109 42-43 0.25779144350946126 1109 44-45 1.2318723979180888 1109 46-47 2.2837736309295096 1109 48-49 3.063426680694967 1109 50-51 2.1698246493020648 1109 52-53 -1.2076716699226893 1109 54-55 -1.102152838088685 1109 56-57 1.1580528921020914 1109 58-59 1.960731255684621 1109 60-61 1.1035282459021971 1109 62-63 0.5619328187730943 1109 64-65 2.155103353107336 1109 66-67 0.6743447689618804 1109 68-69 -2.709436556377508 1109 70-71 -1.9883284011856865 1109 72-73 -1.7885354041131798 1109 74-75 -0.42962379636871617 1109 76 -4.493489241132721 1110 1 -0.30797245451042343 1110 2 -2.0883781598620743 1110 3 -0.2940565434198632 1110 4 -0.17217265658066339 1110 5 -0.28971734218783496 1110 6 -1.2362992404082291 1110 7 0.18201705586367467 1110 8 -0.0731463148178193 1110 9 1.3578443513714404 1110 10-11 -0.4399834083921803 1110 12-13 0.5855434867961833 1110 14-15 0.6730680142359375 1110 16-17 -0.051049850721476275 1110 18-19 -0.4304009717052324 1110 20-21 1.6042132138358198 1110 22-23 -2.005115478124729 1110 24-25 -0.4910622742628874 1110 26-27 1.575120477607257 1110 28-29 2.390498485180075 1110 30-31 -0.32791802793349945 1110 32-33 0.803734302858274 1110 34-35 2.3539479192792854 1110 36-37 2.3460178212293883 1110 38-39 -0.5420527678866485 1110 40-41 -0.9516877587407251 1110 42-43 -0.7511371279191081 1110 44-45 1.7318723979180888 1110 46-47 0.2703807737866484 1110 48-49 2.219676680694967 1110 50-51 -0.5935682078407964 1110 52-53 1.0021497586487413 1110 54-55 0.5764185904827457 1110 56-57 1.5419814635306608 1110 58-59 0.12590982711319043 1110 60-61 0.018706817330766512 1110 62-63 -1.1434243240840516 1110 64-65 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-1.6379571297020519 2116 76 -3.7018225744660533 2117 1 1.334884688346719 2117 2 1.4116218401379257 2117 3 -0.2940565434198632 2117 4 -0.17217265658066339 2117 5 0.49599694352644974 2117 6 0.19227218816320146 2117 7 1.0391599130065288 2117 8 -1.1445748862463887 2117 9 -0.6421556486285596 2117 10-11 -4.297126265535034 2117 12-13 -6.521599370346671 2117 14-15 -0.6840748429069095 2117 16-17 -2.1581927078643375 2117 18-19 -0.5018295431338018 2117 20-21 0.6042132138358198 2117 22-23 -0.9694011924104444 2117 24-25 -3.1339194171200333 2117 26-27 -3.139165236678462 2117 28-29 -3.359501514819925 2117 30-31 1.3506534006379312 2117 32-33 -3.4819799828560036 2117 34-35 -2.5031949378635723 2117 36-37 -2.5825536073420388 2117 38-39 4.350804374970497 2117 40-41 -1.8802591873121521 2117 42-43 -1.2154228422048234 2117 44-45 -2.6252704592247653 2117 46-47 -0.4439049404990598 2117 48-49 -5.2446090335907485 2117 50-51 -1.7007110649836505 2117 52-53 1.6450069015058801 2117 54-55 -1.1735814095172543 2117 56-57 -3.100875679326478 2117 58-59 -0.5169473157439484 2117 60-61 -5.874150325526372 2117 62-63 -2.9291386097983363 2117 64-65 -1.7109680754640948 2117 66-67 -0.4640480881809772 2117 68-69 -4.0889008420917925 2117 70-71 0.9581001702428829 2117 72-73 4.53289316731539 2117 74-75 3.659661917916999 2117 76 2.060082187438706 2118 1 -1.9865438830818505 2118 2 1.1616218401379257 2118 3 -4.615485114848433 2118 4 -0.06502979943780929 2118 5 0.49599694352644974 2118 6 0.5137007595917709 2118 7 1.2177313415779594 2118 8 1.1768536851821807 2118 9 -2.4992985057714137 2118 10-11 0.3635880201792503 2118 12-13 -3.4501707989181014 2118 14-15 0.5302108713787987 2118 16-17 -7.015335565007192 2118 18-19 -1.466115257419517 2118 20-21 -5.77078678616418 2118 22-23 0.8698845218752709 2118 24-25 -3.7232051314057486 2118 26-27 -10.085593808107035 2118 28-29 -5.163072943391356 2118 30-31 -0.23863231364778414 2118 32-33 1.8394485885725658 2118 34-35 1.0503764907078548 2118 36-37 -9.903982178770612 2118 38-39 -6.488481339315218 2118 40-41 -5.73740204445501 2118 42-43 -0.42970855649053874 2118 44-45 -2.643127602081911 2118 46-47 -0.7474763690704904 2118 48-49 2.219676680694967 2118 50-51 2.2635746493020648 2118 52-53 -8.42642166992269 2118 54-55 0.8978471619113151 2118 56-57 3.2205528921020914 2118 58-59 -7.570518744315379 2118 60-61 -3.052721754097803 2118 62-63 -6.500567181226906 2118 64-65 0.0926033531073358 2118 66-67 -7.6069052310381196 2118 68-69 2.821813443622492 2118 70-71 -7.9883284011856865 2118 72-73 -5.03853540411318 2118 74-75 4.695376203631284 2118 76 10.381510758867279 2119 1 1.7634561169181495 2119 2 1.4116218401379257 2119 3 1.1345148851515674 2119 4 0.6849702005621907 2119 5 0.49599694352644974 2119 6 0.7637007595917709 2119 7 1.4677313415779594 2119 8 1.093520351848845 2119 9 1.1673681608952506 2119 10-11 1.6552546868459217 2119 12-13 2.5498292010818986 2119 14-15 1.8635442047121273 2119 16-17 0.817997768326137 2119 18-19 2.283884742580483 2119 20-21 -4.229120119497516 2119 22-23 1.0782178552086066 2119 24-25 1.3184615352609228 2119 26-27 2.289406191892965 2119 28-29 -0.6214062767246915 2119 30-31 2.636367686352216 2119 32-33 -1.74388474476077 2119 34-35 1.2587098240411905 2119 36-37 -6.153982178770612 2119 38-39 -5.530148005981882 2119 40-41 -0.4040687111216741 2119 42-43 0.7369581101761327 2119 44-45 2.731872397918089 2119 46-47 2.1275236309295096 2119 48-49 2.469676680694967 2119 50-51 3.680241315968736 2119 52-53 1.740244996743975 2119 54-55 2.0645138285779865 2119 56-57 -7.029447107897909 2119 58-59 2.7211479223512924 2119 60-61 -4.802721754097803 2119 62-63 0.9994328187730943 2119 64-65 -1.2823966468926642 2119 66-67 0.5597614356285447 2119 68-69 2.530146776955828 2119 70-71 1.3866715988143135 2119 72-73 2.46146459588682 2119 74-75 0.6120428702979481 2119 76 -3.368489241132721 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 3.0 23 4.0 24 8.0 25 24.0 26 55.0 27 100.0 28 178.0 29 284.0 30 354.0 31 470.0 32 648.0 33 798.0 34 1031.0 35 734.0 36 227.0 37 110.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 3.9578361177406527 22.235481304693717 11.793953858393 62.012728719172635 2 2.167859984089101 4.455051710421639 81.24502784407319 12.13206046141607 3 2.247414478918059 23.4884645982498 66.2291169451074 8.035003977724742 4 2.486077963404932 52.24741447891807 22.215592680986475 23.050914876690534 5 2.426412092283214 36.71439936356404 56.98090692124105 3.8782816229116945 6 2.863961813842482 59.70564836913286 11.475735879077167 25.95465393794749 7 3.32140015910899 35.68019093078759 8.571996817820207 52.426412092283215 8 2.6252983293556085 58.98965791567223 21.26093874303898 17.124105011933175 9 3.162291169451074 16.726332537788384 3.8583929992044554 76.25298329355608 10-11 3.4307875894988062 33.900159108989655 39.011535401750194 23.657517899761338 12-13 12.17183770883055 6.533412887828162 36.73428798727128 44.560461416070005 14-15 15.38385043754972 13.26571201272872 9.95425616547335 61.39618138424821 16-17 34.546539379474936 29.902545743834523 3.221957040572793 32.32895783611774 18-19 34.21837708830549 19.192521877486076 2.9534606205250595 43.635640413683376 20-21 15.771678599840891 7.667064439140811 3.202068416865553 73.35918854415274 22-23 15.682179793158312 41.50755767700875 2.764518695306285 40.04574383452665 24-25 34.56642800318218 41.865552903739065 3.7291169451073984 19.83890214797136 26-27 43.76491646778043 37.102227525855206 11.933174224343675 7.199681782020684 28-29 41.616945107398564 20.14717581543357 7.816229116945108 30.41964996022275 30-31 36.49562450278441 16.040175019888625 39.926412092283215 7.537788385043755 32-33 19.89856801909308 35.01392203659507 33.1145584725537 11.972951471758154 34-35 29.50477326968974 43.59586316626889 19.262132060461415 7.637231503579953 36-37 10.431583134447097 32.706841686555286 16.378281622911693 40.48329355608592 38-39 12.012728719172634 13.60381861575179 32.3090692124105 42.074383452665074 40-41 7.1897374701670635 13.345266507557676 43.16825775656324 36.296738265712015 42-43 38.912092283214 6.334526650755768 42.939538583929995 11.813842482100238 44-45 9.496817820206841 30.190930787589497 38.07677008750994 22.235481304693717 46-47 39.35958631662689 6.066030230708035 20.55489260143198 34.019490851233094 48-49 31.831742243436757 3.8981702466189336 16.626889419252187 47.643198090692124 50-51 9.626093874303898 4.345664280031822 44.55051710421639 41.477724741447894 52-53 11.097852028639618 4.007557677008751 69.87867939538583 15.015910898965792 54-55 6.354415274463007 3.8186157517899764 49.4928400954654 40.334128878281625 56-57 38.47454256165474 4.594272076372316 23.965791567223548 32.9653937947494 58-59 8.79077167859984 23.050914876690534 56.652744630071595 11.505568814638027 60-61 29.76332537788385 52.157915672235475 13.295544948289578 4.78321400159109 62-63 4.733492442322991 87.32100238663485 3.052903739061257 4.892601431980907 64-65 2.0584725536992843 89.80708035003978 2.655131264916468 5.479315831344471 66-67 2.167859984089101 87.77844073190136 2.6650755767700876 7.388623707239458 68-69 2.4463007159904535 81.57319013524264 4.116945107398569 11.863564041368338 70-71 3.271678599840891 73.70723945902944 6.404136833731106 16.616945107398568 72-73 5.2008750994431185 58.850437549721555 9.55648369132856 26.392203659506762 74-75 5.688146380270485 56.672633253778834 10.043754972155927 27.59546539379475 76 4.932378679395385 60.10342084327765 8.611774065234686 26.352426412092285 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 0.5 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 0.5 20 1.0 21 2.0 22 1.5 23 2.0 24 3.5 25 4.0 26 6.0 27 6.0 28 5.0 29 6.0 30 7.5 31 7.0 32 6.5 33 8.0 34 13.0 35 16.0 36 18.0 37 22.0 38 35.5 39 97.0 40 426.0 41 929.0 42 1151.0 43 1080.0 44 878.0 45 583.5 46 420.0 47 334.5 48 189.0 49 99.5 50 70.0 51 52.0 52 32.5 53 22.0 54 13.0 55 13.5 56 19.5 57 18.5 58 12.0 59 13.0 60 13.0 61 19.0 62 26.0 63 22.0 64 13.0 65 9.0 66 8.5 67 7.0 68 8.0 69 8.0 70 5.5 71 4.0 72 3.0 73 1.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 5028.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.505966587112173 #Duplication Level Percentage of deduplicated Percentage of total 1 80.83875632682575 22.235481304693717 2 9.038322487346347 4.972155926809864 3 3.470715835140998 2.863961813842482 4 1.5907447577729574 1.7501988862370723 5 0.7230657989877078 0.994431185361973 6 0.8676789587852495 1.431980906921241 7 0.21691973969631237 0.41766109785202865 8 0.3615328994938539 0.7955449482895784 9 0.3615328994938539 0.8949880668257757 >10 1.8076644974692697 7.836117740652347 >50 0.3615328994938539 6.205250596658711 >100 0.21691973969631237 8.591885441527445 >500 0.07230657989877079 12.49005568814638 >1k 0.07230657989877079 28.520286396181383 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT 1434 28.520286396181383 No Hit ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT 628 12.49005568814638 RNA PCR Primer, Index 24 (95% over 23bp) TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG 190 3.7788385043754973 No Hit CTTTACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC 128 2.5457438345266508 RNA PCR Primer, Index 24 (95% over 21bp) CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC 114 2.267303102625298 No Hit CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATATCGTATGCCGT 81 1.6109785202863964 No Hit CTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC 71 1.4120922832140015 No Hit CTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGT 55 1.0938743038981702 No Hit TATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCT 53 1.0540970564836913 RNA PCR Primer, Index 24 (95% over 22bp) CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCGT 52 1.0342084327764518 No Hit TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC 32 0.6364359586316627 No Hit ACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTTCT 31 0.6165473349244232 RNA PCR Primer, Index 24 (96% over 25bp) CTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG 27 0.5369928400954653 No Hit CTTATACACATCTCCGAGCCCACGCGACGTGATAGCATCTCGTATGCCGT 23 0.4574383452665076 No Hit ATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCGTCTT 21 0.41766109785202865 No Hit ATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGTCTT 17 0.3381066030230708 No Hit TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATATCGTATGCCG 17 0.3381066030230708 No Hit CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTAGTATGCCGT 16 0.31821797931583135 No Hit CTTATACACATCTCCGAGCCCACGAGCCGTGATAGCATCTCGTATGCCGT 16 0.31821797931583135 No Hit ATACACATCTCCGAGCCCACGAGACGTGATAGCATATCGTATGCCGTCTT 15 0.29832935560859186 No Hit TCTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC 15 0.29832935560859186 No Hit TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCG 15 0.29832935560859186 No Hit CTTATACACATCTCCGAGCCCACGAGACGTGATCGCATCTCGTATGCCGT 14 0.27844073190135243 No Hit CTTATACACATCTCCGAGCACACGAGACGTGATAGCATCTCGTATGCCGT 14 0.27844073190135243 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCC 14 0.27844073190135243 No Hit ATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTTCTGCTT 13 0.25855210819411295 RNA PCR Primer, Index 34 (96% over 28bp) CTTATACACATCTCCGAGCCCACGTGACGTGATAGCATCTCGTATGCCGT 13 0.25855210819411295 No Hit CTTAACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC 11 0.21877486077963404 RNA PCR Primer, Index 24 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGC 10 0.1988862370723946 No Hit CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCAGT 10 0.1988862370723946 No Hit TTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC 10 0.1988862370723946 No Hit CTTATACACATCTCCGAGCCCACGAGACGCGATAGCATCTCGTATGCCGT 10 0.1988862370723946 No Hit CTTATTACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG 10 0.1988862370723946 No Hit CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAGCTCGTATGCCGT 10 0.1988862370723946 No Hit CTTATACACATATCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT 10 0.1988862370723946 No Hit CTTATACACCTCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT 9 0.17899761336515513 No Hit CTTATACACATCTCCGAGCCCACGAGACGTGATAGCCTCTCGTATGCCGT 9 0.17899761336515513 No Hit CTTATAACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG 9 0.17899761336515513 No Hit ATTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT 9 0.17899761336515513 No Hit CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCCCGTATGCCGT 9 0.17899761336515513 No Hit CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGACGT 8 0.15910898965791567 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATATCGTATGCC 8 0.15910898965791567 No Hit CTTATACACATCTCCGAGCCCAAGAGACGTGATAGCATCTCGTATGCCGT 8 0.15910898965791567 No Hit CTTATACACATCTCCGAGCCCACGAGACGTGCTAGCATCTCGTATGCCGT 8 0.15910898965791567 No Hit CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAAATCGTATGCCGT 8 0.15910898965791567 No Hit CTTATACACATCTCCGAGCCAACGAGACGTGATAGCATCTCGTATGCCGT 7 0.13922036595067622 No Hit CTTATACACATCTACGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT 7 0.13922036595067622 No Hit CGTCAGATGTGTATAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA 7 0.13922036595067622 No Hit CTTATACACATCTCCGAGCCCACGAGACGTGATAGCTTCTCGTATGCCGT 6 0.11933174224343676 No Hit CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCACGTATGCCGT 6 0.11933174224343676 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCC 6 0.11933174224343676 No Hit CTTTACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCGTC 6 0.11933174224343676 No Hit ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCCT 6 0.11933174224343676 RNA PCR Primer, Index 24 (95% over 21bp) CATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTTCTGCT 6 0.11933174224343676 RNA PCR Primer, Index 24 (96% over 28bp) CTTATCACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC 6 0.11933174224343676 No Hit ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTCTGCCGTCTT 6 0.11933174224343676 No Hit TCTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT 6 0.11933174224343676 No Hit ATACACATCTCCGAGCCCACGAGACGTGATAGCCTCTCGTATGCCGTCTT 6 0.11933174224343676 No Hit CTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTTCTGCTTGA 6 0.11933174224343676 RNA PCR Primer, Index 24 (96% over 30bp) TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCG 6 0.11933174224343676 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.059665871121718374 0.0 2 0.0 0.0 0.0 0.059665871121718374 0.0 3 0.0 0.0 0.0 0.059665871121718374 0.0 4 0.0 0.0 0.0 0.059665871121718374 0.0 5 0.0 0.0 0.0 0.059665871121718374 0.0 6 0.0 0.0 0.0 0.059665871121718374 0.0 7 0.0 0.0 0.0 0.059665871121718374 0.0 8 0.0 0.0 0.0 0.059665871121718374 0.0 9 0.0 0.0 0.0 0.07955449482895784 0.0 10 0.0 0.0 0.0 0.07955449482895784 0.0 11 0.0 0.0 0.0 0.11933174224343675 0.0 12 0.0 0.0 0.0 0.19888623707239458 0.0 13 0.0 0.0 0.0 0.35799522673031026 0.0 14 0.0 0.0 0.0 0.35799522673031026 0.0 15 0.0 0.0 0.0 0.35799522673031026 0.0 16 0.0 0.0 0.0 0.35799522673031026 0.0 17 0.0 0.0 0.0 0.35799522673031026 0.0 18 0.0 0.0 0.0 0.5767700875099443 0.0 19 0.0 0.0 0.0 0.7159904534606205 0.0 20 0.0 0.0 0.0 0.7955449482895783 0.0 21 0.0 0.0 0.0 0.8750994431185362 0.0 22 0.0 0.0 0.0 0.8750994431185362 0.0 23 0.0 0.0 0.0 0.8750994431185362 0.0 24 0.0 0.0 0.0 0.8750994431185362 0.0 25 0.0 0.0 0.0 0.9148766905330151 0.0 26 0.0 0.0 0.0 0.9148766905330151 0.0 27 0.0 0.0 0.0 0.9148766905330151 0.0 28 0.0 0.0 0.0 0.954653937947494 0.0 29 0.0 0.0 0.0 0.9745425616547335 0.0 30 0.0 0.0 0.0 0.994431185361973 0.0 31 0.0 0.0 0.0 1.0739856801909309 0.0 32 0.0 0.0 0.0 1.1137629276054097 0.0 33 0.0 0.0 0.0 1.1336515513126493 0.0 34 0.0 0.0 0.0 1.1933174224343674 0.0 35 0.0 0.0 0.0 1.2529832935560858 0.0 36 0.0 0.0 0.0 1.6109785202863962 0.0 37 0.0 0.0 0.0 1.8297533810660302 0.0 38 0.0 0.0 0.0 2.1479713603818618 0.0 39 0.0 0.0 0.0 2.386634844868735 0.0 40 0.0 0.0 0.0 2.5656324582338903 0.0 41 0.0 0.0 0.0 2.8838504375497216 0.0 42 0.0 0.0 0.0 3.0628480509148766 0.0 43 0.0 0.0 0.0 3.202068416865553 0.0 44 0.0 0.0 0.0 3.4009546539379474 0.0 45 0.0 0.0 0.0 3.5003977724741446 0.0 46 0.0 0.0 0.0 3.5600636435958632 0.0 47 0.0 0.0 0.0 3.858392999204455 0.0 48 0.0 0.0 0.0 4.0771678599840895 0.0 49 0.0 0.0 0.0 4.176610978520286 0.0 50 0.0 0.0 0.0 4.2959427207637235 0.0 51 0.0 0.0 0.0 4.41527446300716 0.0 52 0.0 0.0 0.0 4.733492442322992 0.0 53 0.0 0.0 0.0 4.8727128082736675 0.0 54 0.0 0.0 0.0 5.031821797931583 0.0 55 0.0 0.0 0.0 5.290373906125696 0.0 56 0.0 0.0 0.0 5.429594272076372 0.0 57 0.0 0.0 0.0 5.52903739061257 0.0 58 0.0 0.0 0.0 5.6682577565632455 0.0 59 0.0 0.0 0.0 5.767700875099443 0.0 60 0.0 0.0 0.0 5.827366746221162 0.0 61 0.0 0.0 0.0 5.86714399363564 0.0 62 0.0 0.0 0.0 5.8870326173428795 0.0 63 0.0 0.0 0.0 5.986475735879077 0.0 64 0.0 0.0 0.0 6.046141607000796 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCGTA 15 0.0020972362 70.0 38 TAGCATA 15 0.0020972362 70.0 33 AGCATAT 15 0.0020972362 70.0 34 ATATCGT 15 0.0020972362 70.0 37 CATATCG 15 0.0020972362 70.0 36 GCATATC 15 0.0020972362 70.0 35 CTTATAC 310 0.0 60.967743 1 TTATACA 315 0.0 60.0 2 TATACAC 325 0.0 57.07692 3 GAGAAGT 20 0.006556063 52.5 24 AGAAGTG 20 0.006556063 52.5 25 GAAGTGA 20 0.006556063 52.5 26 ACGAGAA 20 0.006556063 52.5 22 AGTGATA 20 0.006556063 52.5 28 GCTTGAC 20 0.006556063 52.5 56 AAGTGAT 20 0.006556063 52.5 27 CGAGAAG 20 0.006556063 52.5 23 CTTGACA 20 0.006556063 52.5 57 CACGAGA 450 0.0 42.0 21 CTGCTTG 425 0.0 42.0 54 >>END_MODULE