FastQCFastQC Report
Wed 25 May 2016
SRR1780575_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780575_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71572
Sequences flagged as poor quality0
Sequence length50
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTG1040.14530822109204716No Hit
CTTATACACATCTGACGCTGTCTCTTATACACATCTGACGCTGCCGACGA980.1369250544905829No Hit
GATGTGTATAAGCTGTCTCTTATACACATCTGACGCTGCCGACGAGAATG770.107583971385458No Hit
GCGTCAGATGTGTATAAGCTGTCTCTTATACACATCTGACGCTGCCGACG730.1019951936511485No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAT207.828413E-443.9755251
TACTCAT207.8554463E-443.9447945
AAGGACA207.8554463E-443.9447945
CTTTTAC250.002344456735.180421
TATATAC250.002352521835.1558343
GTCAGAT250.002352521835.1558343
TCAGATG250.002352521835.1558344
CGTCAGA250.002352521835.1558342
TTTACAC1100.031.959852
CTTTACA1050.031.411091
ACTCTGC300.005666408729.3786512
TGACGCC453.9746734E-529.37864913
TCCTCAC300.005744563429.296532
GTCGTGT407.0185604E-427.46549633
ACTCATC407.0185604E-427.4654966
CATCTGT508.304899E-526.3668779
TTACACA1250.026.3668773
ATTTTTG609.680594E-625.6344623
CGCATCT551.5867397E-423.9698897
GCATCTG551.5867397E-423.9698898
Produced by FastQC (version 0.11.5)