##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780575_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 71572 Sequences flagged as poor quality 0 Sequence length 50 %GC 54 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 19.683493544961717 16.0 10.0 29.0 7.0 31.0 2 20.45252333314704 22.0 10.0 30.0 7.0 33.0 3 20.429301961660986 22.0 10.0 30.0 7.0 33.0 4 22.37109484155815 23.0 10.0 33.0 7.0 37.0 5 22.509375174649303 24.0 10.0 33.0 7.0 37.0 6 22.248351310568378 23.0 11.0 33.0 8.0 37.0 7 22.040644386072767 22.0 10.0 33.0 8.0 37.0 8 21.945369697647124 22.0 10.0 33.0 8.0 37.0 9 22.45598837534231 22.0 10.0 34.0 8.0 39.0 10 22.425669256133684 22.0 10.0 34.0 8.0 39.0 11 22.817819817805844 23.0 10.0 34.0 8.0 39.0 12 22.490988095903425 23.0 10.0 34.0 8.0 39.0 13 22.59531660425865 23.0 10.0 34.0 8.0 39.0 14 22.933703124126755 23.0 10.0 34.0 8.0 40.0 15 22.587226848488235 22.0 10.0 34.0 8.0 40.0 16 22.613633823282846 22.0 10.0 34.0 8.0 40.0 17 22.665441792879896 22.0 10.0 34.0 8.0 40.0 18 22.417258145643547 22.0 10.0 34.0 7.0 40.0 19 22.450595204828705 21.0 10.0 34.0 7.0 40.0 20 22.501145699435533 22.0 10.0 34.0 7.0 40.0 21 22.244802436707094 21.0 10.0 34.0 7.0 40.0 22 22.29883194545353 21.0 10.0 34.0 7.0 40.0 23 22.17464930419717 21.0 10.0 34.0 7.0 40.0 24 21.78322528363047 19.0 8.0 33.0 7.0 40.0 25 21.712583133068797 19.0 9.0 33.0 7.0 40.0 26 21.433102330520317 18.0 8.0 32.0 7.0 40.0 27 21.199505393170515 18.0 8.0 32.0 7.0 40.0 28 21.11044821997429 18.0 8.0 32.0 7.0 39.0 29 20.85526462862572 18.0 8.0 32.0 7.0 39.0 30 20.687922651316157 17.0 8.0 31.0 7.0 39.0 31 20.57739059967585 17.0 8.0 31.0 7.0 39.0 32 20.38461968367518 17.0 8.0 31.0 7.0 39.0 33 20.290071536355 16.0 8.0 31.0 7.0 39.0 34 19.917858939249985 16.0 8.0 30.0 7.0 38.0 35 19.783378975018163 16.0 8.0 30.0 7.0 38.0 36 19.660328061253004 16.0 8.0 30.0 7.0 38.0 37 19.513301291007657 15.0 8.0 30.0 7.0 38.0 38 19.520804225115967 15.0 8.0 30.0 7.0 38.0 39 19.214217850556082 15.0 8.0 30.0 7.0 38.0 40 18.642513832224893 14.0 8.0 29.0 7.0 38.0 41 18.79465433409713 15.0 8.0 29.0 7.0 38.0 42 18.830101156876992 15.0 8.0 29.0 7.0 38.0 43 18.872072877661655 15.0 8.0 29.0 7.0 38.0 44 18.922595428379815 15.0 8.0 29.0 7.0 38.0 45 18.9883613703683 15.0 8.0 29.0 7.0 37.0 46 18.762183535460796 14.0 8.0 29.0 7.0 37.0 47 18.621807410719274 14.0 8.0 28.0 7.0 37.0 48 18.51427932711116 14.0 8.0 28.0 7.0 37.0 49 18.313698094226794 13.0 8.0 28.0 7.0 37.0 50 18.238417258145642 13.0 8.0 28.0 7.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.016943396649896414 1101 2 0.03365310182262249 1101 3 0.17144876663062192 1101 4 0.3799809653433144 1101 5 -0.23893412983670714 1101 6 -1.0708360261347032 1101 7 0.3719188143821235 1101 8 0.7052012545954724 1101 9 0.5522410854068731 1101 10 0.0751568266769631 1101 11 0.3241441696421212 1101 12 -0.7298709556947003 1101 13 0.5018208226911369 1101 14 -0.34348408939541386 1101 15 -1.1064520021184805 1101 16 0.05679629949812437 1101 17 -0.6541609020252324 1101 18 0.4615435220316719 1101 19 0.6862249038090873 1101 20 0.838759305744226 1101 21 0.11704915459388232 1101 22 0.4725599105049447 1101 23 -0.3936773051138367 1101 24 0.2280425690975214 1101 25 -0.08655291818648791 1101 26 -1.052269192492453 1101 27 -1.378325246989668 1101 28 0.15964175259380298 1101 29 -0.5102829231270718 1101 30 -1.3839958440777558 1101 31 -0.8623918115169218 1101 32 -0.8375654836463831 1101 33 1.2040584220712134 1101 34 -0.4608316307302971 1101 35 -1.4995529425090872 1101 36 -1.1145793987012702 1101 37 -0.6751778846307239 1101 38 -1.0106460248471834 1101 39 -1.2425663242585578 1101 40 -1.0592615916553392 1101 41 -0.9383310107854612 1101 42 -1.4645977025276409 1101 43 -0.9688153010086786 1101 44 -0.8087010961059029 1101 45 -0.4103162406853116 1101 46 -0.024253024443680715 1101 47 -0.5334543591127314 1101 48 -0.8242607982194521 1101 49 -0.8776514190117268 1101 50 -0.43750089652309754 1102 1 -0.30523168493818176 1102 2 0.8534729216424424 1102 3 0.13541273059458447 1102 4 0.34394492930727694 1102 5 0.941246050343473 1102 6 0.6228576675589892 1102 7 0.15570259816590593 1102 8 1.389885939280159 1102 9 1.7234122565780439 1102 10 0.9220036735238111 1102 11 0.9908108363087891 1102 12 0.7746335488098062 1102 13 0.060379381249695996 1102 14 0.8907501448388224 1102 15 0.3710254753589979 1102 16 1.110850353552177 1102 17 0.6341273862630565 1102 18 1.1822642427523924 1102 19 1.1546933722775563 1102 20 0.5054259724108938 1102 21 1.6035356410803665 1102 22 1.7158031537481868 1102 23 0.9126290011924709 1102 24 0.5433578844128384 1102 25 0.6792128475792794 1102 26 0.38917224894898794 1102 27 1.405458536794118 1102 28 0.6371192300712778 1102 29 0.03025761741346855 1102 30 -0.22183368191559438 1102 31 0.4078784587533484 1102 32 0.6038759577950579 1102 33 0.7085629265757198 1102 34 1.0797089098102433 1102 35 -0.7788322217883632 1102 36 -0.1956604797823509 1102 37 0.8022995928467545 1102 38 0.8812458670447079 1102 39 0.9826589009666691 1102 40 0.45425192185817664 1102 41 1.0616689892145388 1102 42 0.2200869821570457 1102 43 0.5717252395318617 1102 44 1.074181786776979 1102 45 1.6887828584137878 1102 46 0.9216929215022667 1102 47 0.6106897850314112 1102 48 2.220784246825591 1102 49 0.8340602926999843 1102 50 0.9408774818552779 1103 1 -0.7628074425139388 1103 2 0.11154007970959867 1103 3 0.3691555643374187 1103 4 0.4619629473252971 1103 5 -0.471448462351038 1103 6 -0.2218597771584534 1103 7 -0.8874587449954348 1103 8 -0.24279219339797464 1103 9 -0.9573412241754369 1103 10 -0.6110020594819225 1103 11 -0.8105814650835121 1103 12 0.1689824431587006 1103 13 -1.1319220110516959 1103 14 0.24480419889287575 1103 15 0.6267175310510531 1103 16 0.17358581628764114 1103 17 -1.0983050461693757 1103 18 -0.3016833411951936 1103 19 -0.4207366031524167 1103 20 -0.43806297107805037 1103 21 0.5859271234718513 1103 22 0.5727237106687433 1103 23 -1.021932833369366 1103 24 -0.3256838846289334 1103 25 0.40173537010180027 1103 26 -0.7577564979797593 1103 27 -0.26038912905355005 1103 28 -0.8822091892571393 1103 29 -0.5696604825046307 1103 30 -0.6450933051752159 1103 31 -0.4094843586094683 1103 32 0.26800372192282396 1103 33 -0.4533535353407423 1103 34 -0.1647628346615022 1103 35 0.9413970984409552 1103 36 -1.0259635100853828 1103 37 -0.2667421761950166 1103 38 -0.3675665817677398 1103 39 -0.2792575609497945 1103 40 -1.0173287497224948 1103 41 -0.7662589387133885 1103 42 -0.8887582266881644 1103 43 -0.5325335647269434 1103 44 0.00194591454110693 1103 45 -0.12243745280652618 1103 46 0.14683624664559147 1103 47 -0.6686713943297669 1103 48 0.18335590939725321 1103 49 -0.9277742691345772 1103 50 -0.0842658432880441 1104 1 -0.6385650182715175 1104 2 -1.4618423936728746 1104 3 -0.7294521342702787 1104 4 -0.4668658815035336 1104 5 -1.2569521478547223 1104 6 -0.9987639540626319 1104 7 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43 0.5627162305228524 1104 44 0.5336412462364386 1104 45 0.3464405160714428 1104 46 -0.627856628047283 1104 47 -0.9478687735271478 1104 48 -0.7251616991203527 1104 49 0.26649272513241584 1104 50 0.003940544918343392 1105 1 1.7190107393042418 1105 2 1.793358261527782 1105 3 1.478246473428328 1105 4 1.0346902200525676 1105 5 1.3467333558307786 1105 6 0.023594768296089086 1105 7 0.9125412550045624 1105 8 1.0390259884202067 1105 9 1.388113321279107 1105 10 1.779907031427168 1105 11 1.8348730803710325 1105 12 0.859891534067792 1105 13 2.622623443493758 1105 14 2.153895107983786 1105 15 1.6539902583237804 1105 16 1.937222179924003 1105 17 2.3926040447397163 1105 18 2.3164984769866237 1105 19 2.5428997604839445 1105 20 1.1619370289219475 1105 21 0.7586543961991232 1105 22 1.9090873470323793 1105 23 2.478067166630634 1105 24 2.274316115371068 1105 25 1.1017353701017996 1105 26 2.1604253202020587 1105 27 2.2487017800373614 1105 28 2.2086999016519506 1105 29 2.366703153859003 1105 30 2.1549066948247813 1105 31 1.8996065504814403 1105 32 1.4589128128319153 1105 33 1.3011919192047117 1105 34 0.8897826198839525 1105 35 2.068669825713684 1105 36 0.6104001262782539 1105 37 0.9150760056231668 1105 38 0.6869788727777149 1105 39 1.8571060754138422 1105 40 1.5190348866411405 1105 41 0.9701046976502461 1105 42 1.0657872278572889 1105 43 0.8401937080003314 1105 44 0.2928550054501997 1105 45 0.48665345628438317 1105 46 0.8468362466455908 1105 47 0.7404195147611397 1105 48 0.9469922730336187 1105 49 0.24495300359269478 1105 50 1.2339159748937725 1106 1 0.09060164839515039 1106 2 0.3946569628264811 1106 3 1.0665581617400157 1106 4 0.33517723303958036 1106 5 0.864428161025586 1106 6 -0.09215198495066446 1106 7 0.2605932030565121 1106 8 0.850551962446179 1106 9 -0.027633431967647226 1106 10 -0.02772283870270087 1106 11 0.5814639894619411 1106 12 1.1016123132885696 1106 13 -0.32218175131143667 1106 14 0.328732770321448 1106 15 0.6913279206614433 1106 16 0.4956637383655611 1106 17 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1107 4 -0.1336122739136414 1107 5 0.8597663405451819 1107 6 1.2054934006372022 1107 7 1.6999105229048048 1107 8 0.7632415958216541 1107 9 1.2700119536202195 1107 10 1.1632537731809904 1107 11 0.7014861133557453 1107 12 1.2989100376880636 1107 13 0.6294617379426697 1107 14 0.5200254378309275 1107 15 1.0679081987903913 1107 16 0.24534768273977292 1107 17 0.8831108830341634 1107 18 1.3722506893760062 1107 19 1.487388899663351 1107 20 1.1299177207964455 1107 21 1.3811805426190737 1107 22 0.7554268482391393 1107 23 1.0772626614013525 1107 24 1.3680409745826516 1107 25 0.5203194408982625 1107 26 0.9938122872173452 1107 27 0.7725955853470943 1107 28 -0.12171039601498279 1107 29 1.5433725022097704 1107 30 1.354423991687213 1107 31 1.698319342114587 1107 32 0.7710608417941032 1107 33 0.9264533834042297 1107 34 -0.3204345965279529 1107 35 0.7481549423669414 1107 36 0.5978498447014253 1107 37 0.07831011664488585 1107 38 -0.7228360910195519 1107 39 -0.3658223236207512 1107 40 -0.14975031046263965 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1.125633294186997 2211 16 0.7145366086145799 2211 17 0.917291284105854 2211 18 1.4440214127664426 2211 19 1.3418989264555883 2211 20 1.3901271873873355 2211 21 1.1551514770998743 2211 22 0.940780424597019 2211 23 0.6593849314346407 2211 24 -0.8803961448457791 2211 25 0.6421023425788661 2211 26 0.4137197322120656 2211 27 1.4652155415052484 2211 28 1.2036123286744704 2211 29 1.4432669570283139 2211 30 0.7036973537071844 2211 31 1.1709993778375711 2211 32 1.5773448395208227 2211 33 0.1157040126158897 2211 34 -0.1476652533437388 2211 35 0.014291176839620334 2211 36 1.46794808290878 2211 37 0.6996464810193288 2211 38 0.9295143189828856 2211 39 0.9494330145297738 2211 40 0.8602358874751701 2211 41 1.1062181255067927 2211 42 0.6150783037538723 2211 43 0.618175359376476 2211 44 1.442146081346781 2211 45 0.5285216798039833 2211 46 1.6880372474796204 2211 47 0.9642727257703143 2211 48 1.4296444832087651 2211 49 1.369807048630225 2211 50 0.6149001283549893 2212 1 1.4020146918734113 2212 2 1.2660855342550548 2212 3 0.9976140623611336 2212 4 0.040223816890513575 2212 5 0.05068592500073876 2212 6 0.5852548473474748 2212 7 -0.30524530626025737 2212 8 1.7410022730051864 2212 9 1.3019473133739687 2212 10 0.3874169128500924 2212 11 0.978746598157592 2212 12 1.4397334313009935 2212 13 1.4048368822289348 2212 14 1.0404563728059202 2212 15 0.5840297840154776 2212 16 0.7751668439556241 2212 17 1.4190862581784494 2212 18 0.3034549987257549 2212 19 0.4172080608792008 2212 20 0.07181845184685187 2212 21 0.3491682301912178 2212 22 0.454541892486926 2212 23 1.3144308029942735 2212 24 1.4423003050943883 2212 25 0.8799962396670189 2212 26 0.2817691937198461 2212 27 -0.0904286547452493 2212 28 0.7632453561974053 2212 29 0.6125529562305445 2212 30 0.7628118331647045 2212 31 0.5699622817067365 2212 32 1.7324305993931794 2212 33 0.9541563460821827 2212 34 1.1368181930064836 2212 35 0.6655077703777152 2212 36 0.2854989405075017 2212 37 0.04906810048482413 2212 38 -0.24503693749896627 2212 39 0.4282523204731312 2212 40 0.5716040566016147 2212 41 0.23808888737356781 2212 42 0.6887121290430613 2212 43 0.27497631669598377 2212 44 -0.2755244411901181 2212 45 -0.5291568203954569 2212 46 0.30375324269301984 2212 47 0.4273760365002701 2212 48 -0.3142725491007674 2212 49 -0.38152920984603966 2212 50 0.26316498675148026 2213 1 -0.36320269943093564 2213 2 0.32695509947244616 2213 3 0.24109232323069918 2213 4 -0.37716748745731365 2213 5 -0.4101836402166512 2213 6 -0.6930060222177445 2213 7 -0.13133226278199928 2213 8 -0.40682381395133405 2213 9 -0.976313556191247 2213 10 -0.17780047845425528 2213 11 -0.6299490540163184 2213 12 -0.6646143947859642 2213 13 -0.6386413786406315 2213 14 0.5013259380233102 2213 15 -1.2942310855497396 2213 16 -0.06831141691393938 2213 17 -1.424392002691114 2213 18 -0.8530667404046781 2213 19 -0.30453106955557985 2213 20 -0.7368772003270614 2213 21 -0.4508317698087829 2213 22 -0.08458854229568402 2213 23 -0.9290474578752921 2213 24 -0.14030839055778443 2213 25 -1.1373950646808062 2213 26 -0.7791003714975453 2213 27 -1.1339069156148156 2213 28 -1.8976242090199875 2213 29 -1.9613600872477157 2213 30 -0.6632751233570353 2213 31 -1.256124674815002 2213 32 -0.11104766147638756 2213 33 0.4498085199952264 2213 34 -0.8457905026456913 2213 35 -1.2301444035353306 2213 36 -1.366674972535975 2213 37 -1.6291927690803902 2213 38 -0.34068911141200786 2213 39 -1.4760955056138236 2213 40 -1.089265508615778 2213 41 -2.031476330017739 2213 42 -1.1721574361743308 2213 43 -0.690241074608366 2213 44 -1.0581331368422902 2213 45 -0.45089595083024037 2213 46 -0.6179858877417601 2213 47 -1.2508848330649442 2213 48 0.12920571176879747 2213 49 -1.0423987750634325 2213 50 -0.9542263175963477 2214 1 -0.8095051892116878 2214 2 0.21822228639180707 2214 3 0.21857281375466897 2214 4 0.6867492721116193 2214 5 -0.1751780660806439 2214 6 0.11442615912748266 2214 7 0.15131359377690146 2214 8 -0.9215023721081543 2214 9 -1.009089475923691 2214 10 -1.7465109949908566 2214 11 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-0.45945745964811735 2214 47 0.22076139510302184 2214 48 0.5430295690709137 2214 49 -1.9025994439597547 2214 50 -0.5155789746011763 2215 1 1.255374375667877 2215 2 1.1751764433459648 2215 3 0.2964282916101446 2215 4 0.26196294732529424 2215 5 1.0467333558307814 2215 6 0.49632204102336175 2215 7 1.3489048913681998 2215 8 0.13902598842020808 2215 9 0.28811332127910916 2215 10 0.8799070314271695 2215 11 1.0439639894619397 2215 12 1.3053460795223373 2215 13 0.804441625311938 2215 14 1.1448041988928743 2215 15 -0.21873701440349436 2215 16 1.3099494526512778 2215 17 1.6289676811033509 2215 18 0.9801348406229877 2215 19 0.5792633968475833 2215 20 0.6073915743764928 2215 21 0.05865439619912394 2215 22 0.6727237106687447 2215 23 0.22352171208518 2215 24 0.6197706608256119 2215 25 1.3017353701018024 2215 26 1.0513344111111493 2215 27 1.5396108709464507 2215 28 1.5177908107428593 2215 29 1.8576122447680952 2215 30 0.6549066948247813 2215 31 0.8996065504814403 2215 32 0.3407309946500945 2215 33 1.2830101010228923 2215 34 0.8079644380657705 2215 35 0.6868516438955012 2215 36 0.6194910353691618 2215 37 1.3514396419868007 2215 38 1.559706145504986 2215 39 1.7480151663229329 2215 40 0.19176215936841245 2215 41 0.4519228794684267 2215 42 0.42033268240274424 2215 43 0.4311027989094214 2215 44 1.229218641813837 2215 45 0.8775625471934738 2215 46 1.9104726102819534 2215 47 2.049510423852052 2215 48 0.5197195457608927 2215 49 0.4722257308654214 2215 50 0.6793705203483178 2216 1 -0.5932575749058202 2216 2 -0.834885339531354 2216 3 0.24894373732947628 2216 4 0.1969144036359758 2216 5 -0.29342551442517717 2216 6 -0.1863787533279151 2216 7 -0.8052875181640147 2216 8 -0.054354417581560455 2216 9 -0.21894757898567718 2216 10 -0.8648414240008968 2216 11 0.1342552515978639 2216 12 0.1923716752857949 2216 13 -0.009768436470938724 2216 14 -1.7833511409129486 2216 15 -0.14345016533023625 2216 16 -0.7512155958924112 2216 17 -0.798305046169375 2216 18 -0.8359287074793933 2216 19 0.12189358219621482 2216 20 -0.27389703992182746 2216 21 0.17154053918235235 2216 22 -1.652254224017927 2216 23 -1.052559488267864 2216 24 -1.2212708219634436 2216 25 0.03668682641248111 2216 26 -0.5554617053937037 2216 27 0.6584105179014372 2216 28 -1.081414837977352 2216 29 -0.07583877023631658 2216 30 -1.7894887155900463 2216 31 -0.4394931847348005 2216 32 -2.1492072224725867 2216 33 -1.3544126703804586 2216 34 -0.8439331788803912 2216 35 -0.6933778353631048 2216 36 -0.18271549596358128 2216 37 0.13475826511125177 2216 38 -0.768802239314077 2216 39 -1.0803166959895094 2216 40 -1.6330392528116384 2216 41 0.8521876632283565 2216 42 -0.6904351905010486 2216 43 -0.9015538648152024 2216 44 -0.8827848886362979 2216 45 -0.9798955287112037 2216 46 -0.6984417763023352 2216 47 -1.2323960192194399 2216 48 -1.1572442671252503 2216 49 -2.1311281967603506 2216 50 -0.6914150048061387 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 5.0 5 1.0 6 15.0 7 405.0 8 2160.0 9 4653.0 10 4557.0 11 3666.0 12 3314.0 13 3425.0 14 3379.0 15 3225.0 16 3163.0 17 2837.0 18 2597.0 19 2454.0 20 2197.0 21 2077.0 22 1784.0 23 1738.0 24 1661.0 25 1567.0 26 1536.0 27 1384.0 28 1347.0 29 1229.0 30 1213.0 31 1132.0 32 1125.0 33 1078.0 34 1057.0 35 1134.0 36 1216.0 37 1658.0 38 1588.0 39 3995.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.871713102830928 19.28359628510686 20.110215956137406 28.734474655924807 2 20.44098537035226 16.008774994061508 43.85261363477581 19.697626000810427 3 20.152016878344583 26.37520783557586 32.05069092230094 21.422084363778627 4 17.396467892471918 21.126977030123513 34.38774939920639 27.08880567819818 5 18.180293969708824 26.34130665623428 39.663555580394565 15.814843793662325 6 25.08942044374895 26.10238640809255 28.209355613927233 20.59883753423126 7 29.8482646845135 23.6782540658358 23.430950651092605 23.042530598558095 8 30.577600178840886 25.029341083105123 23.491030011736434 20.902028726317553 9 28.582406527692395 16.119432180182194 20.699435533448835 34.59872575867658 10 25.84390543788074 22.894427988598892 30.25065668138378 21.011009892136588 11 35.96249772946387 17.750702119632802 24.762117676647712 21.524682474255613 12 26.522656457404743 20.390113037767748 26.542217999413154 26.545012505414356 13 31.07148845788385 15.253479403051815 26.205913587837458 27.469118551226874 14 34.83692462480087 17.37933540147005 21.897096224253097 25.88664374947598 15 27.48920471219553 22.357774703391605 22.187286015735268 27.965734568677597 16 31.611946147171604 18.714137766954963 22.929437120385142 26.744478965488288 17 37.57807805709654 18.443888601651693 19.912524628648885 24.06550871260288 18 31.5728340120632 16.773724057824985 21.165176259848582 30.48826567026324 19 31.42229129040839 17.360381928402425 23.80472370392149 27.4126030772677 20 36.05445988346033 17.534177498879426 22.873711340206185 23.537651277454056 21 29.456093905394237 19.928282276477425 20.71269487750557 29.90292894062277 22 32.634345467762685 16.317172733881343 19.48218978918526 31.56629200917072 23 38.83158630328442 18.76030747728861 19.16282320055905 23.245283018867923 24 30.077502028482694 26.04711675666601 18.32209507288548 25.55328614196581 25 34.85286922485496 19.72041657929685 18.2526036206053 27.17411057524289 26 34.71861108781347 23.415527412004806 18.872487349380748 22.993374150800975 27 31.197931227285437 24.591836734693878 20.90718479172491 23.30304724629578 28 35.84858185274753 22.2178732683786 19.90997665543705 22.023568223436822 29 31.56261358235245 21.98534962395504 22.408924427544942 24.043112366147565 30 34.54448826606249 20.830186568207882 20.940672988168163 23.684652177561468 31 33.37806855672986 19.33260638595314 22.633227087177765 24.65609797013924 32 31.881343137529004 20.73839013615903 22.340425531914892 25.039841194397074 33 35.22290411418506 19.01360211370976 23.1585422112871 22.604951560818083 34 31.13885471423778 20.10261715691757 24.418409573873166 24.34011855497148 35 33.373408077390856 22.185564704402164 21.560678288342444 22.88034892986454 36 35.230052986983935 18.205712528136228 24.118165168397947 22.446069316481886 37 31.248602102437932 19.74809885931559 24.980429434131064 24.02286960411541 38 33.42140211085483 18.230236946949045 25.660166352135317 22.68819459006081 39 32.378023207046 19.316370753529988 26.35677338179785 21.94883265762617 40 27.01094716801523 29.588151300501163 24.614329310972366 18.786572220511243 41 32.20796286976458 16.087904713974165 26.613263993737068 25.09086842252419 42 25.344610652872923 19.738571228855026 32.331888718020416 22.584929400251642 43 26.292320165506446 15.470099807095531 30.452347004389274 27.78523302300875 44 26.365224683765465 15.81102802432036 32.77797190579356 25.04577538612062 45 25.56156609496652 15.698690261528354 35.98356187361094 22.756181769894187 46 26.020964360587 18.72816212438854 29.913347309573723 25.337526205450732 47 30.088334777625587 18.378385933525283 31.179940178346797 20.353339110502333 48 29.31400777122411 20.370111536633775 27.875772230453133 22.440108461688986 49 28.463613366689493 20.399435367779624 28.41190199997205 22.725049265558834 50 30.350553505535053 21.934753438443476 24.468858324946886 23.245834731074584 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 1.0 6 1.0 7 2.0 8 3.0 9 8.0 10 13.0 11 10.0 12 7.0 13 9.0 14 11.0 15 22.0 16 33.0 17 33.5 18 34.0 19 71.0 20 108.0 21 119.0 22 130.0 23 169.5 24 209.0 25 240.0 26 271.0 27 363.0 28 455.0 29 570.5 30 686.0 31 750.5 32 815.0 33 986.5 34 1158.0 35 1380.5 36 1603.0 37 1774.5 38 1946.0 39 2114.0 40 2282.0 41 2435.0 42 2588.0 43 2939.0 44 3290.0 45 3580.0 46 3870.0 47 4153.0 48 4436.0 49 4534.0 50 4632.0 51 4765.0 52 4898.0 53 4891.5 54 4885.0 55 4732.5 56 4580.0 57 4482.5 58 4385.0 59 4086.0 60 3787.0 61 3558.5 62 3330.0 63 3130.0 64 2930.0 65 2730.0 66 2530.0 67 2377.5 68 2225.0 69 2070.5 70 1916.0 71 1822.0 72 1728.0 73 1575.0 74 1422.0 75 1342.5 76 1263.0 77 1160.0 78 1057.0 79 892.5 80 728.0 81 610.0 82 492.0 83 408.0 84 324.0 85 273.5 86 223.0 87 183.0 88 143.0 89 99.5 90 56.0 91 49.0 92 42.0 93 25.5 94 9.0 95 9.0 96 9.0 97 12.0 98 15.0 99 14.0 100 13.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10618677695188063 2 0.006985972167886883 3 0.0013971944335773767 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.00419158330073213 12 0.00419158330073213 13 0.011177555468619014 14 0.013971944335773766 15 0.018163527636505895 16 0.16486894316213044 17 0.012574749902196388 18 0.1606773598613983 19 0.2025931928687196 20 0.2514949980439278 21 0.25289219247750516 22 0.055887777343095066 23 0.030738277538702286 24 0.1257474990219639 25 0.05169619404236294 26 0.04750461074163081 27 0.044710221874476055 28 0.04890180517520818 29 0.05309338847594031 30 0.09780361035041636 31 0.055887777343095066 32 0.05309338847594031 33 0.054490582909517686 34 0.06147655507740457 35 0.054490582909517686 36 0.06287374951098194 37 0.050298999608785554 38 0.05169619404236294 39 0.058682166210249825 40 0.19281283183367798 41 0.055887777343095066 42 0.058682166210249825 43 0.04750461074163081 44 0.037724249706589164 45 0.043313027440898676 46 0.030738277538702286 47 0.03632705527301179 48 0.03632705527301179 49 0.029341083105124913 50 0.03912144414016654 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 71572.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.50489018051752 #Duplication Level Percentage of deduplicated Percentage of total 1 97.99613597534689 85.7514111663779 2 0.7169202765492024 1.2546806013524843 3 0.17563748423254402 0.4610741630805343 4 0.10218908173529834 0.35768177499580844 5 0.07504510689935973 0.3283406918906835 6 0.07983522010570183 0.41915833007321296 7 0.08622203771415798 0.5281394958922484 8 0.08941544651838605 0.6259431062426647 9 0.05109454086764917 0.40239199687028443 >10 0.611537786009676 8.83585759794333 >50 0.014370339619026328 0.8900128541887888 >100 0.0015967044021140365 0.14530822109204716 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTG 104 0.14530822109204716 No Hit CTTATACACATCTGACGCTGTCTCTTATACACATCTGACGCTGCCGACGA 98 0.1369250544905829 No Hit GATGTGTATAAGCTGTCTCTTATACACATCTGACGCTGCCGACGAGAATG 77 0.107583971385458 No Hit GCGTCAGATGTGTATAAGCTGTCTCTTATACACATCTGACGCTGCCGACG 73 0.1019951936511485 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.12435030458838651 0.0 2 0.0 0.0 0.0 0.12993908232269602 0.0 3 0.0 0.0 0.0 0.12993908232269602 0.0 4 0.0 0.0 0.0 0.12993908232269602 0.0 5 0.0 0.0 0.0 0.12993908232269602 0.0 6 0.0 0.0 0.0 0.12993908232269602 0.0 7 0.0 0.0 0.0 0.12993908232269602 0.0 8 0.0 0.0 0.0 0.13273347118985077 0.0 9 0.0 0.0 0.0 0.17045772089643996 0.0 10 0.0 0.0 0.0 0.27245291454758847 0.0 11 0.0 0.0 0.0 0.3716537193315822 0.0 12 0.0 0.0 0.0 0.4806348851506176 0.0 13 0.0 0.0 0.0 0.6734477169842955 0.0 14 0.0 0.0 0.0 0.6734477169842955 0.0 15 0.0 0.0 0.0 0.674844911417873 0.0 16 0.0 0.0 0.0 0.6860224668864919 0.0 17 0.0 0.0 0.0 0.690214050187224 0.0 18 0.0 0.0 0.0 0.8956016319230984 0.0 19 0.0 0.0 0.0 1.1401106577991393 0.0 20 0.0 0.0 0.0 1.194601240708657 0.0 21 0.0 0.0 0.0 1.2407086570167105 0.0 22 0.0 0.0 0.0 1.2812272955904545 0.0 23 0.0 0.0 0.0 1.2979936287933829 0.0 24 0.0 0.0 0.0 1.3161571564298888 0.0 25 0.0 0.0 0.0 1.3692505449058292 0.0 26 0.0 0.0 0.0 1.3888112669759123 0.0 27 0.0 0.0 0.0 1.4083719890459956 0.0 28 0.0 0.0 0.0 1.461465377521936 0.0 29 0.0 0.0 0.0 1.479628905158442 0.0 30 0.0 0.0013971944335773767 0.0 1.5243391270329179 0.0 31 0.0 0.0013971944335773767 0.0 1.631923098418376 0.0 32 0.0 0.0013971944335773767 0.0 1.721343542167328 0.0 33 0.0 0.0013971944335773767 0.0 1.8051752081819705 0.0 34 0.0 0.0013971944335773767 0.0 1.9504834292740179 0.0 35 0.0 0.0013971944335773767 0.0 2.1838148996814395 0.0 36 0.0 0.0013971944335773767 0.0 2.593192868719611 0.0 37 0.0 0.0013971944335773767 0.0 2.9872016989884314 0.0 38 0.0 0.0013971944335773767 0.0 3.5265187503492985 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTATAT 20 7.828413E-4 43.975525 1 TACTCAT 20 7.8554463E-4 43.944794 5 AAGGACA 20 7.8554463E-4 43.944794 5 CTTTTAC 25 0.0023444567 35.18042 1 TATATAC 25 0.0023525218 35.155834 3 GTCAGAT 25 0.0023525218 35.155834 3 TCAGATG 25 0.0023525218 35.155834 4 CGTCAGA 25 0.0023525218 35.155834 2 TTTACAC 110 0.0 31.95985 2 CTTTACA 105 0.0 31.41109 1 ACTCTGC 30 0.0056664087 29.37865 12 TGACGCC 45 3.9746734E-5 29.378649 13 TCCTCAC 30 0.0057445634 29.29653 2 GTCGTGT 40 7.0185604E-4 27.465496 33 ACTCATC 40 7.0185604E-4 27.465496 6 CATCTGT 50 8.304899E-5 26.366877 9 TTACACA 125 0.0 26.366877 3 ATTTTTG 60 9.680594E-6 25.634462 3 CGCATCT 55 1.5867397E-4 23.969889 7 GCATCTG 55 1.5867397E-4 23.969889 8 >>END_MODULE