FastQCFastQC Report
Wed 25 May 2016
SRR1780575_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780575_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71572
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT3836153.59777566646174No Hit
ATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCTT68739.60291734197731No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG52417.32269602637903No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC42685.963225842508243No Hit
CTTTACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTC11111.5522830157044654No Hit
CTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTC7311.0213491309450622No Hit
TATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCT6860.9584753814340803No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC5060.7069803833901526No Hit
ACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCTTCT3270.4568825797798022Illumina PCR Primer Index 8 (95% over 22bp)
CATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCTTCTGCT2750.38422846923377857Illumina PCR Primer Index 8 (96% over 25bp)
CTTAATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG2560.35768177499580844No Hit
ATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCTTCTGCTT2100.2934108310512491RNA PCR Primer, Index 39 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATATCGTATGCCGT2070.28921924775051694No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC1950.2724529145475884No Hit
TTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTC1910.2668641368132789No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATAATCTCGTATGCCGTC1650.23053708154026714No Hit
TCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCTTCTGCTTG1410.1970044151344101Illumina PCR Primer Index 8 (96% over 27bp)
CTTATTACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG1060.1481026099592019No Hit
CGTCAGATGTGTATAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA970.13552786005700554No Hit
CTTATAACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG870.12155591572123178No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTA850.11876152685407701No Hit
CTTATACACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAA780.10898116581903537No Hit
CTTATACACATCTGACGCCTGTCTCTTATACACATCTCCGAGCCCACGAG760.10618677695188063No Hit
CTTAACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTC720.10059799921757111No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATTA207.754577E-444.0601841
TCTTAAT408.029019E-944.0601841
ACGCCTG207.808247E-443.99860415
CATCTGA207.808247E-443.9986049
TTATTAC207.808247E-443.9986042
ACATCTG207.808247E-443.9986048
ATTACAC207.808247E-443.9986044
TCTGACG207.808247E-443.99860411
TATTACA207.808247E-443.9986043
ATCTGAC207.808247E-443.99860410
TGACGCC207.808247E-443.99860413
CTTTACA1150.040.2288631
TTTACAC1200.038.498782
TTTATAC800.038.498783
TCTTTAT700.037.7658731
CTTTATA700.037.713092
CCGTCTT8200.037.02321244
CGTCAGA301.2785937E-436.716821
AGATGTG301.2891772E-436.6655045
ATGGTGG250.002338441635.1988835
Produced by FastQC (version 0.11.5)