FastQCFastQC Report
Wed 25 May 2016
SRR1780574_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780574_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93814
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGA207.892266E-569.9973410
CCGTGCG207.892266E-569.997349
CCCGTGC207.892266E-569.997348
CGAGTTC150.002218857469.9973360
ATGGCAC150.002218857469.9973351
GCGAAGC150.002218857469.9973320
TAGCCCG252.821329E-669.997335
TGGCACA150.002218857469.9973352
GCCGAGT150.002218857469.9973358
GAGTTCC150.002218857469.9973361
GCAGCGG150.002218857469.9973325
AGCAGCG150.002218857469.9973324
TGCCGAG150.002218857469.9973357
GATGCCT308.322373E-658.33111237
CGGGGTG252.3801424E-455.99786431
CAGCGGT200.006933293352.49826
GAGCTGC200.006933293352.49851
CGAGTGA200.006933293352.49864
TAAACAG200.006933293352.49837
ACAGGCG200.006933293352.49840
Produced by FastQC (version 0.11.5)