FastQCFastQC Report
Wed 25 May 2016
SRR1780574_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780574_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93814
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT4270.45515594687360095TruSeq Adapter, Index 12 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT2420.2579572345278956No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC1970.20998998017353487No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAAGTT252.8206887E-670.0000166
GGTCGGC150.002218522470.017
CAACGGT150.002218522470.013
GCTGAAC150.002218522470.056
AACGGTC150.002218522470.014
TCGTGGA150.002218522470.065
CGGTCGG150.002218522470.016
GAACATC150.002218522470.024
CTGGATA150.002218522470.044
GTCGGCG150.002218522470.018
ACGGTCG150.002218522470.015
CGGAACA308.320496E-658.33333262
CAACGGA308.320496E-658.33333259
TGCGGTC308.320496E-658.33333254
AACGGAA308.320496E-658.33333260
ACGGAAC308.320496E-658.33333261
ACCAACG308.320496E-658.33333257
CCAACGG308.320496E-658.33333258
CGGCGAA200.00693224952.520
TAGCGGG200.00693224952.561
Produced by FastQC (version 0.11.5)