##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780574_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 93814 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.918061270172895 34.0 33.0 34.0 27.0 34.0 2 32.799187754492934 34.0 34.0 34.0 31.0 34.0 3 33.17883258362291 34.0 34.0 34.0 32.0 34.0 4 33.26731617882192 34.0 34.0 34.0 33.0 34.0 5 33.30327030080798 34.0 34.0 34.0 33.0 34.0 6 36.881957916728844 38.0 37.0 38.0 35.0 38.0 7 36.48805082397084 38.0 37.0 38.0 34.0 38.0 8 36.3756688767135 38.0 37.0 38.0 34.0 38.0 9 36.18255271068284 38.0 37.0 38.0 34.0 38.0 10-11 35.76367066749099 38.0 36.5 38.0 31.5 38.0 12-13 35.71526104845759 38.0 36.0 38.0 31.0 38.0 14-15 35.967792653548514 38.0 36.5 38.0 32.5 38.0 16-17 36.149956296501585 38.0 37.0 38.0 33.0 38.0 18-19 36.09813567271409 38.0 37.0 38.0 33.0 38.0 20-21 35.82805871191933 38.0 36.0 38.0 31.5 38.0 22-23 35.801388918498304 38.0 36.5 38.0 31.5 38.0 24-25 34.95709595582749 37.0 35.0 38.0 28.5 38.0 26-27 35.250991323256656 37.0 36.0 38.0 30.0 38.0 28-29 35.04913445754365 37.0 35.5 38.0 29.0 38.0 30-31 34.608587204468414 37.0 34.5 38.0 26.5 38.0 32-33 35.25804250964674 37.0 36.0 38.0 31.0 38.0 34-35 35.32871959409043 37.5 35.5 38.0 29.0 38.0 36-37 35.56082247852133 38.0 36.0 38.0 31.0 38.0 38-39 35.31026819024879 37.5 35.5 38.0 29.0 38.0 40-41 34.487256699426524 37.0 34.0 38.0 26.0 38.0 42-43 34.9517609312043 37.0 35.0 38.0 29.0 38.0 44-45 35.23542328437121 37.0 35.5 38.0 29.0 38.0 46-47 35.52126015306884 38.0 36.0 38.0 31.0 38.0 48-49 35.20304538768201 37.5 35.5 38.0 29.0 38.0 50-51 35.52811414074658 37.5 36.0 38.0 31.0 38.0 52-53 35.401320698403225 38.0 36.0 38.0 31.0 38.0 54-55 35.537217259684056 38.0 36.0 38.0 31.0 38.0 56-57 35.677041806127015 38.0 36.0 38.0 31.0 38.0 58-59 35.821721704649626 38.0 36.5 38.0 31.5 38.0 60-61 35.29700257957234 37.5 35.5 38.0 29.0 38.0 62-63 35.60511224337519 38.0 36.0 38.0 31.0 38.0 64-65 35.67904044172512 38.0 36.0 38.0 31.5 38.0 66-67 34.92805444816339 37.0 35.5 38.0 27.0 38.0 68-69 34.32675826635683 37.0 34.0 38.0 24.5 38.0 70-71 34.31345001812096 37.0 34.0 38.0 25.0 38.0 72-73 34.030283326582385 37.0 34.0 38.0 24.5 38.0 74-75 34.94850448760313 37.0 35.0 38.0 28.0 38.0 76 34.40287163962735 37.0 34.0 38.0 26.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.24860056153061905 1101 2 0.9692241432562483 1101 3 0.312171858912194 1101 4 0.40220885177261323 1101 5 0.2951429624846682 1101 6 -1.1111256180053672 1101 7 -0.22119785570656347 1101 8 0.951209135288785 1101 9 0.53238000397711 1101 10-11 0.528346569479254 1101 12-13 1.0086062271805005 1101 14-15 0.9407989520458386 1101 16-17 0.27427867125918937 1101 18-19 -0.3411533710271897 1101 20-21 -0.25707932719107873 1101 22-23 1.2666211755045254 1101 24-25 -1.3896374754800433 1101 26-27 0.0057357485462219415 1101 28-29 0.9534989515679868 1101 30-31 1.145868912396189 1101 32-33 0.5794616383129565 1101 34-35 0.7932467068636058 1101 36-37 0.6943929939630564 1101 38-39 0.4234875149932549 1101 40-41 1.4783416682530728 1101 42-43 0.5997323669882562 1101 44-45 0.7634136961023685 1101 46-47 1.3134864088581466 1101 48-49 -1.4380450342405524 1101 50-51 -1.9996477800274022 1101 52-53 -2.2223121253298572 1101 54-55 -0.8819198851605421 1101 56-57 -1.2792991875456963 1101 58-59 -1.204687675967044 1101 60-61 -1.4970368891036543 1101 62-63 -0.9662140229927161 1101 64-65 -1.791169895575642 1101 66-67 -1.154242399668746 1101 68-69 -0.6813972312309815 1101 70-71 -0.6643991948277517 1101 72-73 -2.1410786076683053 1101 74-75 -0.5808777197330386 1101 76 -0.6044515168398874 1102 1 0.26894420414229714 1102 2 0.5390364615221728 1102 3 0.5864303838923632 1102 4 0.47320012853708704 1102 5 0.32613423924914287 1102 6 0.7210710513047047 1102 7 0.1613873940951791 1102 8 0.9667629285745392 1102 9 1.111941198796032 1102 10-11 1.2917882824055056 1102 12-13 1.332201257579655 1102 14-15 0.4956918941552786 1102 16-17 0.7118890334056402 1102 18-19 0.20078688802647093 1102 20-21 1.0818157296421234 1102 22-23 1.0238984686581034 1102 24-25 -4.674591215897703 1102 26-27 -0.7386152955986418 1102 28-29 0.6782042119433527 1102 30-31 2.1608993115502955 1102 32-33 0.7458005227961735 1102 34-35 1.2021972751956085 1102 36-37 -2.4964925466132044 1102 38-39 0.2342144512872082 1102 40-41 1.8333720674071898 1102 42-43 1.4390450817648954 1102 44-45 1.5763029374452202 1102 46-47 1.7679352589770971 1102 48-49 -3.063855237782711 1102 50-51 -0.4661717028399863 1102 52-53 0.3036989769699048 1102 54-55 1.0978792160818642 1102 56-57 1.302144111423381 1102 58-59 0.3506916526081554 1102 60-61 -6.0183638782656885 1102 62-63 -2.034963692567132 1102 64-65 0.42639817209552433 1102 66-67 1.87748374360379 1102 68-69 0.03761413540924963 1102 70-71 -0.6905424673627749 1102 72-73 -5.394337925669888 1102 74-75 -1.4424479021279666 1102 76 0.689918031137907 1103 1 1.456221832174645 1103 2 0.9981697810328498 1103 3 0.2836893513400156 1103 4 0.7053460957773936 1103 5 0.44733681026303174 1103 6 0.47719794328065746 1103 7 1.1220094135851255 1103 8 0.763354261769642 1103 9 0.5471723827064636 1103 10-11 0.7714482450843292 1103 12-13 -0.25756859520906517 1103 14-15 0.19101430759090476 1103 16-17 0.6989551748114025 1103 18-19 1.1577721668985461 1103 20-21 0.0666301604942845 1103 22-23 0.8956214626452521 1103 24-25 -2.3441164076870464 1103 26-27 0.1695912148720211 1103 28-29 0.3067839341079832 1103 30-31 1.7298066306462943 1103 32-33 1.8585809499162238 1103 34-35 0.8362715028547427 1103 36-37 -0.4326899341313464 1103 38-39 0.308052311540159 1103 40-41 0.5339774997107369 1103 42-43 0.20740087691314812 1103 44-45 1.8786127031512052 1103 46-47 1.7081011308410865 1103 48-49 -1.7722027393377644 1103 50-51 0.25343435148305105 1103 52-53 0.8512005320186304 1103 54-55 0.13613756151001155 1103 56-57 1.0724945157360466 1103 58-59 0.1675069128196469 1103 60-61 -3.6085401171211764 1103 62-63 -0.536730227918575 1103 64-65 0.7916563101838463 1103 66-67 0.3599386471907664 1103 68-69 0.8111741602900295 1103 70-71 1.3526671532053385 1103 72-73 -4.65009575274847 1103 74-75 -1.6266859282527832 1103 76 -3.6255702541616976 1104 1 0.7933886485867454 1104 2 0.8965150940008044 1104 3 0.8064731189351022 1104 4 0.17328559862255588 1104 5 -0.03378029066539057 1104 6 0.17278045301410572 1104 7 0.7773703000780827 1104 8 0.8730877148993272 1104 9 1.5079241047789438 1104 10-11 -0.06718607656885922 1104 12-13 1.122137155015551 1104 14-15 1.176867107830489 1104 16-17 0.9263548453714492 1104 18-19 1.280252699992289 1104 20-21 0.21217897750537418 1104 22-23 0.8759711182307512 1104 24-25 1.237438698632218 1104 26-27 1.8558932514099098 1104 28-29 2.147413613652759 1104 30-31 1.528185636336623 1104 32-33 1.3683218903175387 1104 34-35 1.1372186427169808 1104 36-37 1.7439348038141418 1104 38-39 2.1596845367572897 1104 40-41 2.7662139477490726 1104 42-43 1.354600637320452 1104 44-45 1.5198713135135904 1104 46-47 1.7032344042762446 1104 48-49 0.4900763861489139 1104 50-51 1.0476317159634334 1104 52-53 1.238741712012633 1104 54-55 0.6844604126630571 1104 56-57 1.1396441114233795 1104 58-59 -0.280718603802093 1104 60-61 1.0608668909650731 1104 62-63 0.6859337433303025 1104 64-65 1.195714411411764 1104 66-67 0.8726333162533635 1104 68-69 2.6883192636143747 1104 70-71 2.772384883064575 1104 72-73 2.415021048689084 1104 74-75 0.36571449103443143 1104 76 0.5420547832746649 1105 1 1.6931801290752766 1105 2 0.9467906376409587 1105 3 0.8183214954617597 1105 4 0.23156680207802083 1105 5 -0.025633135467295176 1105 6 0.30127315483120753 1105 7 0.8992797430331052 1105 8 -0.7248270899859932 1105 9 1.1394850222647932 1105 10-11 0.7989443970683183 1105 12-13 -0.09375873417122449 1105 14-15 0.5760218590964996 1105 16-17 0.6500329806112433 1105 18-19 1.1333544277253793 1105 20-21 0.8244183281160318 1105 22-23 0.7776951277630673 1105 24-25 0.8686011949086492 1105 26-27 1.0380685567420542 1105 28-29 1.499264224317038 1105 30-31 1.8331863810491598 1105 32-33 1.731719268929396 1105 34-35 0.12564953339317952 1105 36-37 0.6961987299386578 1105 38-39 0.3652847750652981 1105 40-41 1.073165839318925 1105 42-43 0.756149639405642 1105 44-45 1.690041852685468 1105 46-47 1.5460255868797574 1105 48-49 0.46095812579741136 1105 50-51 1.0872578702678766 1105 52-53 1.1192898204427806 1105 54-55 1.4016036953559308 1105 56-57 1.3662191784475084 1105 58-59 0.9034860432041611 1105 60-61 1.1357998668363933 1105 62-63 -0.22557206542751373 1105 64-65 1.023700708701007 1105 66-67 1.0719444571529735 1105 68-69 0.6503054119611136 1105 70-71 1.814963822593917 1105 72-73 2.2068664463655807 1105 74-75 0.17138175347113105 1105 76 0.9423824567926857 1106 1 -1.280088053922217 1106 2 0.5127337319936345 1106 3 0.4151648752075019 1106 4 0.4326045950383275 1106 5 0.09650644768586858 1106 6 0.46722489745855 1106 7 -0.5944934992050719 1106 8 0.9143063528921545 1106 9 -0.24458485579453537 1106 10-11 0.6978676868720015 1106 12-13 1.8413575851230775 1106 14-15 0.11750330854734159 1106 16-17 0.6685143435793321 1106 18-19 1.1070896175550047 1106 20-21 0.7844460025949758 1106 22-23 1.1491342006680298 1106 24-25 0.05674873447451034 1106 26-27 1.3019057962127718 1106 28-29 1.2769387032584874 1106 30-31 1.3117429840068695 1106 32-33 0.5585548652279329 1106 34-35 1.3778548434338305 1106 36-37 1.5405297858929998 1106 38-39 0.41900353317306127 1106 40-41 1.6942157398637647 1106 42-43 1.2442063720874756 1106 44-45 1.873052317097823 1106 46-47 1.4423025046346751 1106 48-49 1.6961695761130713 1106 50-51 0.4663593145297398 1106 52-53 1.046627016672133 1106 54-55 0.5729908786128703 1106 56-57 0.6827892727136984 1106 58-59 1.0727760198538192 1106 60-61 1.6624797941908867 1106 62-63 1.1894283669862205 1106 64-65 -0.030450463140205386 1106 66-67 1.0925705922390208 1106 68-69 2.384959048560617 1106 70-71 1.1290605103047255 1106 72-73 1.2862576078288654 1106 74-75 0.31777542293407635 1106 76 0.5653522743140869 1107 1 1.6122210403004935 1107 2 0.18432112036615678 1107 3 0.35134129219310495 1107 4 0.625168837793936 1107 5 0.1904193326867798 1107 6 0.4440610556506428 1107 7 0.7806659686279929 1107 8 0.1356112553889659 1107 9 -0.26053163910053456 1107 10-11 1.128294149419844 1107 12-13 0.3254563640550998 1107 14-15 0.6644141888097792 1107 16-17 0.8842879903808694 1107 18-19 1.0501914947192148 1107 20-21 1.1568352863565963 1107 22-23 0.39671028960551524 1107 24-25 0.8220667588958648 1107 26-27 -0.21955401789329443 1107 28-29 1.5687648377582164 1107 30-31 1.1979831881257041 1107 32-33 1.7115893102798765 1107 34-35 0.6061216558996492 1107 36-37 1.8084357454337265 1107 38-39 0.9437711657591734 1107 40-41 1.2748062264684634 1107 42-43 1.6549772851547289 1107 44-45 1.2878327071105815 1107 46-47 1.6038935379862238 1107 48-49 1.4001517157157721 1107 50-51 1.2940064805585365 1107 52-53 0.9204742920503008 1107 54-55 1.2797523147346226 1107 56-57 1.511748631197392 1107 58-59 0.9826994752939484 1107 60-61 1.3831832751458677 1107 62-63 1.2767529523698542 1107 64-65 1.7077671421085583 1107 66-67 0.7139939565546811 1107 68-69 2.18909609977257 1107 70-71 1.4099790450231495 1107 72-73 2.5631848904969914 1107 74-75 0.15785196749394004 1107 76 1.6478928247059272 1108 1 -0.3402198825450071 1108 2 0.883451798507167 1108 3 0.15357494212051392 1108 4 0.6256935793587104 1108 5 0.10376700895621838 1108 6 -0.28478748644237584 1108 7 -0.3582506149545921 1108 8 -1.0440777477769743 1108 9 -0.35494139229708566 1108 10-11 0.4536601566612788 1108 12-13 1.5135587236430013 1108 14-15 0.8765016107544739 1108 16-17 0.06877400601815964 1108 18-19 0.49705266559256245 1108 20-21 1.5102224931572437 1108 22-23 0.5169110907109769 1108 24-25 1.323846507369737 1108 26-27 -0.16511208054743776 1108 28-29 -0.716694573481746 1108 30-31 0.9605944770658965 1108 32-33 1.162499736894766 1108 34-35 0.681435188984608 1108 36-37 1.5926620139964598 1108 38-39 1.8085321459764145 1108 40-41 1.791519245306688 1108 42-43 0.14579430777109081 1108 44-45 1.4129354690003453 1108 46-47 1.3765230179673367 1108 48-49 1.5737537167302733 1108 50-51 1.2553286543879238 1108 52-53 0.6532583958791705 1108 54-55 0.16724335039955207 1108 56-57 0.5928561857267809 1108 58-59 0.6304011072402105 1108 60-61 1.399721380129165 1108 62-63 1.2388491200072949 1108 64-65 1.1639118658321266 1108 66-67 1.2318395426035522 1108 68-69 2.182991606235639 1108 70-71 0.28771426042955994 1108 72-73 -0.07432363655343721 1108 74-75 -0.7390756706442758 1108 76 1.2496227227311465 1109 1 -1.4541327189346234 1109 2 0.6498056922914017 1109 3 0.7495073069692921 1109 4 0.3470462823832392 1109 5 0.23998039309529418 1109 6 -0.4143135640799116 1109 7 -0.48476645205866475 1109 8 0.28368600549761425 1109 9 1.1180950449498823 1109 10-11 0.6456344362516546 1109 12-13 0.48758587296427436 1109 14-15 0.7526149710783585 1109 16-17 1.1226582641748664 1109 18-19 0.8623253495649337 1109 20-21 1.0818157296421234 1109 22-23 0.5192830840427121 1109 24-25 -0.1899758312823252 1109 26-27 -0.015538372521717747 1109 28-29 -0.6879496342104972 1109 30-31 0.4347454653964462 1109 32-33 0.6581082151038657 1109 34-35 -0.13318734018900358 1109 36-37 1.5142766841560302 1109 38-39 1.380368297441045 1109 40-41 0.8118336058687277 1109 42-43 1.0851989279187464 1109 44-45 0.8947644759067543 1109 46-47 1.4002429512847883 1109 48-49 0.9084524545249835 1109 50-51 -0.22924862591690953 1109 52-53 -0.32245486918394306 1109 54-55 -0.626736168533526 1109 56-57 -0.28093281165354256 1109 58-59 0.04299934491584878 1109 60-61 0.8662515063496983 1109 62-63 0.5065747689713334 1109 64-65 1.3063981720955198 1109 66-67 0.751329897449942 1109 68-69 -0.3023858645907467 1109 70-71 0.2971498403295385 1109 72-73 -0.10818407951604314 1109 74-75 -0.40244790212796033 1109 76 0.4899180311379112 1110 1 -1.2939129387148434 1110 2 -0.4447547472690374 1110 3 -0.23955862709664189 1110 4 0.07765067798763425 1110 5 0.5491562172711184 1110 6 0.056510611744265304 1110 7 0.6436401413479231 1110 8 1.096103587915195 1110 9 1.2527653746202105 1110 10-11 -0.6862336956164796 1110 12-13 1.1001133454917422 1110 14-15 1.1663512348146199 1110 16-17 0.8963945279111272 1110 18-19 0.2449352396748239 1110 20-21 1.4762662790926697 1110 22-23 -0.3744257071660755 1110 24-25 -0.6544263807328718 1110 26-27 1.5888297593464173 1110 28-29 0.7793580580972019 1110 30-31 -0.1827270620760757 1110 32-33 1.025663160158814 1110 34-35 1.1588456268439629 1110 36-37 1.6300459149252617 1110 38-39 0.9247639018366556 1110 40-41 -0.8216828776477527 1110 42-43 0.3454736531934728 1110 44-45 1.3476490912913732 1110 46-47 1.17029789633974 1110 48-49 1.9497986083711325 1110 50-51 0.2454491762808999 1110 52-53 0.6462813945523251 1110 54-55 -0.2758570476544051 1110 56-57 1.0360726828519446 1110 58-59 -0.2741709847544769 1110 60-61 0.07336689096507598 1110 62-63 -0.34859006619350197 1110 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2116 52-53 -1.43383512013866 2116 54-55 -1.5636483343818668 2116 56-57 -1.1496289382220155 2116 58-59 -2.256782433589329 2116 60-61 -2.7288494210916596 2116 62-63 0.20348693481967217 2116 64-65 -1.432063366366016 2116 66-67 -1.5690629012407271 2116 68-69 -1.5826913746834919 2116 70-71 -0.1169472682356556 2116 72-73 -1.131610156984678 2116 74-75 -0.5813895278781018 2116 76 -2.1101910796095 2117 1 -0.21857716337906652 2117 2 0.1424552649409776 2117 3 0.060960298422280346 2117 4 -0.2741502988133391 2117 5 -0.329934136819233 2117 6 -0.4908947606611065 2117 7 0.3692506419584234 2117 8 0.4269338687454791 2117 9 -1.9608793140244742 2117 10-11 -0.732998042380828 2117 12-13 -2.2732688278904334 2117 14-15 0.21928163774502707 2117 16-17 -1.831016949500352 2117 18-19 -2.5371618299222476 2117 20-21 -0.73408170625531 2117 22-23 -0.6788365740769393 2117 24-25 -2.024847626154113 2117 26-27 -3.3572477742311193 2117 28-29 -1.834103480364341 2117 30-31 -0.8616647910138013 2117 32-33 -3.2647977678021185 2117 34-35 -0.233187340189005 2117 36-37 -1.8094839996046588 2117 38-39 -0.41125563418288635 2117 40-41 -1.6403031462680246 2117 42-43 0.0041732868930992595 2117 44-45 -1.7941244129821357 2117 46-47 -1.9271074760656361 2117 48-49 -1.3667612206886943 2117 50-51 -0.7940349507032352 2117 52-53 -0.4963010230300995 2117 54-55 -1.4569925787899365 2117 56-57 -0.2824712731920087 2117 58-59 -2.7219579200414117 2117 60-61 -1.3361416560434662 2117 62-63 -0.1296645472679856 2117 64-65 -1.385054819357471 2117 66-67 -0.9428581367380886 2117 68-69 -0.18871065091553874 2117 70-71 -1.3862689630892646 2117 72-73 0.2972860059540423 2117 74-75 -0.5392000388800966 2117 76 -0.8263212851014075 2118 1 -1.3465512913531974 2118 2 -0.2777341552484458 2118 3 -1.6020103645483807 2118 4 -0.0013390260020642586 2118 5 -0.15344996033505964 2118 6 0.10416183439549087 2118 7 0.8351781078858949 2118 8 0.21302765483926578 2118 9 -1.8964302495754097 2118 10-11 -1.7311500405328246 2118 12-13 -1.7227952774168784 2118 14-15 0.12330104176442802 2118 16-17 -3.614223232706628 2118 18-19 -0.792532585293003 2118 20-21 -0.7365072086808198 2118 22-23 0.6861437909034294 2118 24-25 0.3569957556892689 2118 26-27 -2.5147899317732723 2118 28-29 -2.480326626587484 2118 30-31 -0.836716766065777 2118 32-33 -0.5466180496224027 2118 34-35 -0.9199510269526883 2118 36-37 -3.755314445435104 2118 38-39 -0.32439954732679865 2118 40-41 -1.5492890552539365 2118 42-43 -0.3270810443612291 2118 44-45 -4.195256314114033 2118 46-47 -0.8590779080360633 2118 48-49 -0.8395031934306658 2118 50-51 -2.4141550708233552 2118 52-53 -1.762759789488861 2118 54-55 -2.1652392870366413 2118 56-57 -0.8032612939820254 2118 58-59 -1.8426555407390381 2118 60-61 -1.5188628387646474 2118 62-63 -3.2895167071201428 2118 64-65 -0.9861174204200651 2118 66-67 -0.159536353416307 2118 68-69 -3.337243799448686 2118 70-71 -0.2217967986171061 2118 72-73 0.3940751027431375 2118 74-75 -0.79156779124785 2118 76 -0.40959686837698683 2119 1 0.4108489660470589 2119 2 -2.476897604411892 2119 3 -0.3645586270966419 2119 4 -0.8568731315361688 2119 5 -0.5472723541574496 2119 6 -1.2970608168271625 2119 7 -0.9527884300806448 2119 8 0.14610358791519928 2119 9 -0.8674727206178829 2119 10-11 -1.4338527432355193 2119 12-13 -1.1534580830796841 2119 14-15 -1.4336487651853815 2119 16-17 -1.740510233993632 2119 18-19 -1.8604219031823206 2119 20-21 -1.5939718161454266 2119 22-23 -0.8238304690708418 2119 24-25 0.20271647640998935 2119 26-27 0.4739488069654598 2119 28-29 -0.027784799045662112 2119 30-31 0.31370150935249796 2119 32-33 -0.07493207793643109 2119 34-35 -0.8911543731560343 2119 36-37 -1.6032874184080725 2119 38-39 -2.6430932410204875 2119 40-41 -2.2050162109810927 2119 42-43 -0.37952634680653574 2119 44-45 -0.3791366229943449 2119 46-47 -1.1201782941364584 2119 48-49 -1.6924632963907698 2119 50-51 0.21390155723327098 2119 52-53 -0.025147176876252786 2119 54-55 -0.5746665714639292 2119 56-57 -3.177617793338527 2119 58-59 -1.3223852704687644 2119 60-61 -0.8903235852254028 2119 62-63 -1.9878757804792144 2119 64-65 -1.8189681282707753 2119 66-67 -1.7319301758101275 2119 68-69 -0.2146569268618137 2119 70-71 -2.0420992439195516 2119 72-73 -0.20164561797758296 2119 74-75 -1.0192061438862083 2119 76 -1.047627756407877 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 1.0 21 5.0 22 10.0 23 24.0 24 59.0 25 132.0 26 229.0 27 438.0 28 811.0 29 1360.0 30 2224.0 31 3513.0 32 5480.0 33 8004.0 34 11653.0 35 16341.0 36 23040.0 37 20489.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.66723516745902 25.81917411047392 11.682691282750977 25.830899439316095 2 18.0815230136227 20.51932547380988 38.32157247319164 23.077579039375788 3 17.62316924979214 26.541880742746287 29.78340119811542 26.051548809346155 4 12.430980450679003 18.914021361417273 41.48101562666553 27.173982561238198 5 12.300935894429402 38.46227641929776 37.268424755367 11.968362930905835 6 27.729336772763126 40.85957319803015 18.06446798985226 13.346622039354466 7 26.36067111518537 30.594580766196945 25.0548958577611 17.98985226085659 8 22.8665231202166 37.36649114204703 20.340247724220266 19.426738013516108 9 23.93672586181167 14.508495533715651 23.41228388087066 38.14249472360202 10-11 24.614663056686638 26.485918945999533 28.088025241435183 20.811392755878654 12-13 23.759247020700535 23.914874112605794 27.23580702240604 25.090071844287632 14-15 21.789924744707612 25.327776238088134 24.583750826102712 28.298548191101542 16-17 22.42469141066365 28.097618692306053 26.16080755537553 23.31688234165476 18-19 22.06227215554182 27.73200162022726 27.20009806638668 23.005628157844246 20-21 21.521841089816018 27.999552305626025 26.892574669025944 23.58603193553201 22-23 22.204042040633595 27.654721043767456 27.756518216897263 22.384718698701686 24-25 22.21150361353316 28.409938815102226 27.295499605602576 22.08305796576204 26-27 21.11998209222504 28.42273008293005 27.98462915982689 22.472658665018013 28-29 21.83416121261219 27.17238365275972 28.932781887564758 22.060673247063338 30-31 22.240816935638605 27.41754961945978 27.89455731553926 22.447076129362355 32-33 21.215916600933763 28.958364423220416 27.739463193126824 22.086255782718997 34-35 22.183789199906197 28.148250794124547 28.19994883492869 21.46801117104057 36-37 22.646406719679366 27.44313215511544 27.126015306883833 22.78444581832136 38-39 21.796320378621527 28.151981580574326 27.715479565949646 22.3362184748545 40-41 21.6758692732428 27.16065832391754 28.518664591638775 22.644807811200888 42-43 22.005777389302235 27.626473660647665 28.243652333340442 22.12409661670966 44-45 20.862024857697143 27.278977551324964 28.500010659389858 23.358986931588035 46-47 21.739292642889122 26.837678811264844 28.731319419276442 22.691709126569595 48-49 21.345428187690537 26.69271110921611 28.819259385592765 23.142601317500585 50-51 21.038970729315455 26.82222269597288 28.799539514358198 23.339267060353468 52-53 20.922783379879338 26.74174430255612 29.618713624832115 22.716758692732427 54-55 20.33385209030635 27.51561600613981 29.042573603086957 23.107958300466883 56-57 20.49694075511118 26.741211333063298 29.03830984714435 23.723538064681176 58-59 20.15584027970239 27.7938260813951 28.495746903447245 23.554586735455263 60-61 20.450572409235296 27.690429999786815 28.156778306009766 23.70221928496813 62-63 19.312149572558468 27.93079924105144 28.366768286183298 24.390282900206792 64-65 19.984757072505168 28.508538171275077 27.104696527170784 24.40200822904897 66-67 20.079092672735413 28.177564116229988 26.8835141876479 24.859829023386702 68-69 19.74012407529793 28.393949730317438 27.07538320506534 24.79054298931929 70-71 20.429786599015074 28.10134947875584 26.77692028908265 24.691943633146437 72-73 20.40846781930202 27.57744046730765 26.342550152429272 25.671541560961053 74-75 20.052977167586928 27.493764256933932 26.805167672202444 25.6480909032767 76 19.900014923145797 27.527874304474814 27.18144413413776 25.390666638241626 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 0.0 14 1.0 15 2.5 16 1.5 17 0.0 18 1.0 19 6.5 20 9.0 21 7.0 22 18.0 23 49.5 24 86.0 25 102.0 26 179.5 27 309.0 28 449.5 29 538.0 30 660.0 31 927.0 32 1303.5 33 1535.0 34 1775.5 35 2326.5 36 2876.5 37 3116.0 38 3638.5 39 4794.5 40 5925.5 41 6606.5 42 6790.0 43 6890.5 44 7211.5 45 7514.5 46 7597.0 47 7539.5 48 7065.5 49 6111.5 50 5574.0 51 5020.5 52 3852.0 53 2967.0 54 2697.0 55 2445.5 56 1809.0 57 1265.0 58 1106.0 59 826.0 60 436.5 61 288.5 62 250.0 63 185.0 64 101.5 65 79.5 66 69.0 67 62.0 68 57.5 69 44.0 70 34.0 71 33.0 72 26.0 73 15.0 74 6.0 75 1.0 76 2.5 77 2.5 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 93814.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.52563583260494 #Duplication Level Percentage of deduplicated Percentage of total 1 70.95375360320843 30.17353486686422 2 9.931069056272717 8.446500522310103 3 4.7725278856999624 6.088643486046859 4 3.1683168316831685 5.389387511458844 5 2.2759744328863265 4.839362994862174 6 1.6317834315077078 4.163557677958513 7 1.290888582529139 3.8427100432771226 8 0.9976187492166938 3.393949730317437 9 0.789572628148891 3.0219370243247274 >10 4.145882942724652 28.799539514358198 >50 0.03509211680661737 0.9177734666467692 >100 0.007519739315703722 0.9231031615750315 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT 427 0.45515594687360095 TruSeq Adapter, Index 12 (95% over 23bp) CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT 242 0.2579572345278956 No Hit ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 197 0.20998998017353487 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0021318779713049227 0.0 2 0.0 0.0 0.0 0.0031978169569573836 0.0 3 0.0 0.0 0.0 0.0031978169569573836 0.0 4 0.0 0.0 0.0 0.004263755942609845 0.0 5 0.0 0.0 0.0 0.005329694928262306 0.0 6 0.0 0.0 0.0 0.005329694928262306 0.0 7 0.0 0.0 0.0 0.005329694928262306 0.0 8 0.0 0.0 0.0 0.005329694928262306 0.0 9 0.0 0.0 0.0 0.009593450870872152 0.0 10 0.0 0.0 0.0 0.012791267827829534 0.0 11 0.0 0.0 0.0 0.012791267827829534 0.0 12 0.0 0.0 0.0 0.013857206813481996 0.0 13 0.0 0.0 0.0 0.01598908478478692 0.0 14 0.0 0.0 0.0 0.018120962756091843 0.0 15 0.0 0.0 0.0 0.024516596670006607 0.0 16 0.0 0.0 0.0 0.028780352612616453 0.0 17 0.0 0.0 0.0 0.028780352612616453 0.0 18 0.0 0.0 0.0 0.029846291598268914 0.0 19 0.0 0.0 0.0 0.030912230583921375 0.0 20 0.0 0.0 0.0 0.03411004754087876 0.0 21 0.0 0.0 0.0 0.036241925512183686 0.0 22 0.0 0.0 0.0 0.03730786449783614 0.0 23 0.0 0.0 0.0 0.03837380348348861 0.0 24 0.0 0.0 0.0 0.04050568145479353 0.0 25 0.0 0.0 0.0 0.04050568145479353 0.0 26 0.0 0.0 0.0 0.04476943739740337 0.0 27 0.0 0.0 0.0 0.05329694928262306 0.0 28 0.0 0.0 0.0 0.06289040015349522 0.0 29 0.0 0.0 0.0 0.0660882171104526 0.0 30 0.0 0.0 0.0 0.07781354595262967 0.0 31 0.0 0.0 0.0 0.0906048137804592 0.0 32 0.0 0.0 0.0 0.1044620205939412 0.0 33 0.0 0.0 0.0 0.11512141045046581 0.0 34 0.0 0.0 0.0 0.1353742511778626 0.0 35 0.0 0.0 0.0 0.16415460379047903 0.0 36 0.0 0.0 0.0 0.22491312597266933 0.0 37 0.0 0.0 0.0 0.39439742469141065 0.0 38 0.0 0.0 0.0 0.61931055066408 0.0 39 0.0 0.0 0.0 0.8857952970771953 0.0 40 0.0 0.0 0.0 1.275928965825996 0.0 41 0.0 0.0 0.0 1.6649966955891444 0.0 42 0.0 0.0 0.0 2.0796469610079518 0.0 43 0.0 0.0 0.0 2.507088494254589 0.0 44 0.0 0.0 0.0 2.8950902850320848 0.0 45 0.0 0.0 0.0 3.3800925235039547 0.0 46 0.0 0.0 0.0 3.8650947619758247 0.0 47 0.0 0.0 0.0 4.421514912486409 0.0 48 0.0 0.0 0.0 4.996056025753086 0.0 49 0.0 0.0 0.0 5.616432515402819 0.0 50 0.0 0.0 0.0 6.128083228516 0.0 51 0.0 0.0 0.0 6.701558402797024 0.0 52 0.0 0.0 0.0 7.231330078666297 0.0 53 0.0 0.0 0.0 7.706738866267295 0.0 54 0.0 0.0 0.0 8.165092630097853 0.0 55 0.0 0.0 0.0 8.581874773487966 0.0 56 0.0 0.0 0.0 9.056217622103311 0.0 57 0.0 0.0 0.0 9.519901080862132 0.0 58 0.0 0.0 0.0 9.963331698893555 0.0 59 0.0 0.0 0.0 10.46325708316456 0.0 60 0.0 0.0 0.0 11.029270684546017 0.0 61 0.0 0.0 0.0 11.508943228089624 0.0 62 0.0 0.0 0.0 11.96196729699192 0.0 63 0.0 0.0 0.0 12.432046389664656 0.0 64 0.0 0.0 0.0 12.8413669601552 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACAAGTT 25 2.8206887E-6 70.00001 66 GGTCGGC 15 0.0022185224 70.0 17 CAACGGT 15 0.0022185224 70.0 13 GCTGAAC 15 0.0022185224 70.0 56 AACGGTC 15 0.0022185224 70.0 14 TCGTGGA 15 0.0022185224 70.0 65 CGGTCGG 15 0.0022185224 70.0 16 GAACATC 15 0.0022185224 70.0 24 CTGGATA 15 0.0022185224 70.0 44 GTCGGCG 15 0.0022185224 70.0 18 ACGGTCG 15 0.0022185224 70.0 15 CGGAACA 30 8.320496E-6 58.333332 62 CAACGGA 30 8.320496E-6 58.333332 59 TGCGGTC 30 8.320496E-6 58.333332 54 AACGGAA 30 8.320496E-6 58.333332 60 ACGGAAC 30 8.320496E-6 58.333332 61 ACCAACG 30 8.320496E-6 58.333332 57 CCAACGG 30 8.320496E-6 58.333332 58 CGGCGAA 20 0.006932249 52.5 20 TAGCGGG 20 0.006932249 52.5 61 >>END_MODULE