FastQCFastQC Report
Wed 25 May 2016
SRR1780572_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780572_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39141
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCAGG150.002209622269.9936139
CAGCCGG150.002209622269.9936124
GCCGGAA150.002209622269.9936126
GTTAACA150.002209622269.9936157
CGCAGGA150.002209622269.9936140
GCCTATT150.002209622269.9936133
TTAAACG150.002209622269.9936112
CCTGGCA150.002209622269.9936119
AGGATTT150.002209622269.9936143
CAATACG150.002209622269.9936169
CTGGCAG150.002209622269.9936120
TTTCAAC252.363553E-455.9948942
CCCCGAA252.363553E-455.9948913
AACAACT200.006904663552.49520555
AGCCGGA200.006904663552.49520525
GGCAGCC200.006904663552.49520522
CAAGTAG200.006904663552.49520531
GCAGTGC200.006904663552.49520552
CCGGAAG200.006904663552.49520527
TTGTATT200.006904663552.49520537
Produced by FastQC (version 0.11.5)