##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780572_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 39141 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.350987455609207 34.0 27.0 34.0 12.0 34.0 2 30.90516338366419 34.0 32.0 34.0 21.0 34.0 3 30.89936383843029 34.0 32.0 34.0 21.0 34.0 4 31.309700825221636 34.0 33.0 34.0 23.0 34.0 5 31.276206535346567 34.0 34.0 34.0 23.0 34.0 6 33.618916225952326 37.0 34.0 38.0 21.0 38.0 7 33.3717840627475 37.0 34.0 38.0 21.0 38.0 8 32.21910528601722 37.0 31.0 38.0 11.0 38.0 9 33.23900769014588 37.0 34.0 38.0 21.0 38.0 10-11 33.49499246314606 37.0 34.0 38.0 21.0 38.0 12-13 33.794358856442095 38.0 34.0 38.0 21.0 38.0 14-15 33.493140185483256 37.0 34.0 38.0 21.0 38.0 16-17 33.320673973582686 37.0 34.0 38.0 21.0 38.0 18-19 33.34442145065277 37.0 34.0 38.0 16.5 38.0 20-21 33.393308806622215 37.0 34.0 38.0 21.0 38.0 22-23 33.34707851102425 37.0 34.0 38.0 21.0 38.0 24-25 33.773856058864105 38.0 34.0 38.0 21.0 38.0 26-27 33.82617970925628 38.0 34.0 38.0 21.0 38.0 28-29 33.88877391993051 38.0 34.0 38.0 21.0 38.0 30-31 33.840359214123296 38.0 34.0 38.0 21.0 38.0 32-33 33.548018701617224 37.5 34.0 38.0 21.0 38.0 34-35 33.515776295955646 37.0 34.0 38.0 21.0 38.0 36-37 33.48846478117575 37.0 34.0 38.0 21.0 38.0 38-39 33.90227638537595 38.0 34.0 38.0 21.0 38.0 40-41 33.425410694667995 37.5 34.0 38.0 21.0 38.0 42-43 33.71575841189545 37.0 34.0 38.0 21.0 38.0 44-45 32.81594747196035 37.0 32.5 38.0 16.0 38.0 46-47 33.500306583889014 37.0 34.0 38.0 21.0 38.0 48-49 33.287332975652134 37.0 34.0 38.0 21.0 38.0 50-51 33.18535550956797 37.0 34.0 38.0 21.0 38.0 52-53 33.1729516363915 37.0 34.0 38.0 21.0 38.0 54-55 32.89074116655169 37.0 32.5 38.0 16.0 38.0 56-57 32.924107713139676 37.0 32.5 38.0 15.5 38.0 58-59 32.91411818808922 37.0 32.0 38.0 16.0 38.0 60-61 32.895046115326636 37.0 32.5 38.0 15.5 38.0 62-63 32.95770419764442 37.0 32.0 38.0 19.0 38.0 64-65 32.99209269052911 37.0 32.5 38.0 19.0 38.0 66-67 32.92603663677474 37.0 32.5 38.0 19.0 38.0 68-69 32.473046166423956 37.0 31.0 38.0 15.0 38.0 70-71 32.38343425053014 37.0 31.0 38.0 11.0 38.0 72-73 32.55740783321836 37.0 31.0 38.0 11.0 38.0 74-75 32.14001941697964 37.0 30.5 38.0 11.0 38.0 76 31.41590659410848 36.0 28.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.4385494155331138 1101 2 -1.2930109392015865 1101 3 0.0957682950133183 1101 4 -2.5348864990120035 1101 5 0.03557023340700738 1101 6 -1.1207536143812433 1101 7 -0.8037514484112336 1101 8 -1.4048850103708332 1101 9 -0.12385179907728627 1101 10-11 -0.7619412778787193 1101 12-13 -1.4737916949853656 1101 14-15 -0.35697643359154796 1101 16-17 -2.09660367877888 1101 18-19 0.0911316979596819 1101 20-21 0.3307811836017649 1101 22-23 -0.42431606580956327 1101 24-25 -0.7761431697434773 1101 26-27 -0.6559400500836858 1101 28-29 0.1933658843448427 1101 30-31 -1.391562548016367 1101 32-33 0.8113865959001316 1101 34-35 0.1576777865392316 1101 36-37 0.716872221531041 1101 38-39 -0.06587074250609959 1101 40-41 0.12142523638375735 1101 42-43 0.2248758828335724 1101 44-45 -0.14332768681915553 1101 46-47 0.7347133269278459 1101 48-49 -0.45447788442015025 1101 50-51 -0.4788446590023199 1101 52-53 0.03730031786405874 1101 54-55 -0.7755570479932388 1101 56-57 0.8901987488415841 1101 58-59 0.5262280122010452 1101 60-61 -0.25782108248597524 1101 62-63 -1.214626829752678 1101 64-65 -0.57084432756713 1101 66-67 -1.1326834251436004 1101 68-69 0.4423330810523751 1101 70-71 0.7475855744404285 1101 72-73 0.2857261632835204 1101 74-75 -0.17541924273776743 1101 76 -1.9537498320291817 1102 1 1.3909584623116622 1102 2 0.09086379334071992 1102 3 0.2980635683064534 1102 4 0.5442809781325195 1102 5 0.534111373263066 1102 6 0.7163403362120278 1102 7 0.45154907677842004 1102 8 2.0172118579427263 1102 9 0.8270332427433686 1102 10-11 0.004786985105141639 1102 12-13 -2.999710776876043 1102 14-15 -0.7763014676316189 1102 16-17 -2.4722601658436503 1102 18-19 -0.8508542969829449 1102 20-21 0.2751986121176202 1102 22-23 -0.8375625159165452 1102 24-25 0.21607624282217586 1102 26-27 -5.080662866656333 1102 28-29 -0.3766107738928568 1102 30-31 -0.4147097956335628 1102 32-33 -5.497843125943863 1102 34-35 -0.28129323077257595 1102 36-37 0.20092201729061543 1102 38-39 0.18344845609339444 1102 40-41 -0.17341039870670016 1102 42-43 0.3496084251405165 1102 44-45 -5.263391876665487 1102 46-47 0.20923190308034378 1102 48-49 0.6717621467021999 1102 50-51 -5.82542120053121 1102 52-53 -1.484679841637984 1102 54-55 -0.2408361765674485 1102 56-57 -2.080089132893491 1102 58-59 1.1848547385188866 1102 60-61 0.23610033358093574 1102 62-63 -4.97211564515824 1102 64-65 -0.9753862454819426 1102 66-67 -0.05054959903973355 1102 68-69 -7.791230427992559 1102 70-71 -0.23622107796182945 1102 72-73 0.04919630527923857 1102 74-75 -7.4613558309501755 1102 76 -2.368066112908391 1103 1 -0.03464362389787823 1103 2 -1.1891638182561515 1103 3 -0.5514532287483114 1103 4 -0.6810603318843533 1103 5 -0.5425875069332804 1103 6 -1.0177446461472286 1103 7 -0.42289485634015733 1103 8 -0.12728883234719746 1103 9 -0.4855146940567536 1103 10-11 -0.2573314379658882 1103 12-13 -1.8183562746517765 1103 14-15 -2.1263168074076617 1103 16-17 -2.6388884830702217 1103 18-19 -2.2391423779769575 1103 20-21 -2.0718254713307616 1103 22-23 -1.1965899741156605 1103 24-25 -0.7797053187670286 1103 26-27 -1.6555209737279668 1103 28-29 -0.7148144861186552 1103 30-31 -1.0042940877028954 1103 32-33 -5.479703840777425 1103 34-35 -1.9148643306345186 1103 36-37 -0.8503895335607368 1103 38-39 0.086002528803931 1103 40-41 0.18143697052171603 1103 42-43 -0.13938528555129892 1103 44-45 -5.240343863166487 1103 46-47 -1.4339649062462385 1103 48-49 -1.015076325456107 1103 50-51 -0.5959765004238307 1103 52-53 -2.23241722467219 1103 54-55 -0.9032077059431174 1103 56-57 -0.41975012384301635 1103 58-59 -0.5513011136056747 1103 60-61 -0.32805827970331336 1103 62-63 -1.3814192193260588 1103 64-65 -0.461465398726304 1103 66-67 -0.8035408553673875 1103 68-69 -4.101208952306102 1103 70-71 -1.0169773289205608 1103 72-73 0.18150187361794679 1103 74-75 -2.360190007971191 1103 76 -0.8741253244439022 1104 1 -1.7422362930449076 1104 2 0.24324626855658238 1104 3 0.8644210994864956 1104 4 0.6197526762457279 1104 5 0.7782751084725348 1104 6 0.905019581495047 1104 7 1.7059462625795092 1104 8 1.601318029992612 1104 9 0.07263605694227948 1104 10-11 0.011822482386875777 1104 12-13 -0.3769893186093327 1104 14-15 0.07483380579338927 1104 16-17 -0.15518309516887996 1104 18-19 1.6082119006505735 1104 20-21 1.756650482920307 1104 22-23 0.5301221658870929 1104 24-25 0.8403167289110769 1104 26-27 1.311166362636925 1104 28-29 0.05799108092006122 1104 30-31 -0.6448824851122907 1104 32-33 -0.287337849320906 1104 34-35 0.0511340157896214 1104 36-37 0.5746828103830381 1104 38-39 -0.35260846747551966 1104 40-41 0.5669957413636553 1104 42-43 0.37191414947695023 1104 44-45 -3.297530028248474 1104 46-47 0.6248059356994702 1104 48-49 1.1604094124521112 1104 50-51 1.3102519686405145 1104 52-53 -0.12459029894530715 1104 54-55 -0.6137974813323979 1104 56-57 1.0004992905155277 1104 58-59 0.5776433538051009 1104 60-61 0.5076865184226378 1104 62-63 0.8388853142277526 1104 64-65 -0.312850268507205 1104 66-67 0.607269396802927 1104 68-69 -3.276040149770619 1104 70-71 -0.29050633539706894 1104 72-73 0.6600053874666401 1104 74-75 -1.6730603400643425 1104 76 -0.610311076131925 1105 1 -1.9644888900924755 1105 2 -0.4295257380043793 1105 3 -0.9983454396059024 1105 4 -0.06573119472201228 1105 5 0.3302160598120629 1105 6 0.2840518395595666 1105 7 0.6574224790912595 1105 8 1.6986159457843542E-4 1105 9 0.4518050017208495 1105 10-11 0.776926364289551 1105 12-13 -2.36687450176953 1105 14-15 -1.319439020887856 1105 16-17 0.4198579108453302 1105 18-19 0.13910309802619025 1105 20-21 -1.0496644432688669 1105 22-23 -0.49619002656779543 1105 24-25 -0.1670643536476959 1105 26-27 0.20243208160903237 1105 28-29 -1.5506065133937668 1105 30-31 -0.15253694109917149 1105 32-33 -0.021960593990122845 1105 34-35 -0.7946833707604029 1105 36-37 -0.09822588289197398 1105 38-39 -0.2853586041622407 1105 40-41 -0.6387177632836938 1105 42-43 0.020560951007837502 1105 44-45 0.6213464069620258 1105 46-47 0.03630128835118995 1105 48-49 -1.0581526433160633 1105 50-51 -0.32329095098769045 1105 52-53 -1.8456810589234394 1105 54-55 -1.1381277164335444 1105 56-57 -0.41263630270481144 1105 58-59 -0.9540679638548255 1105 60-61 -0.624216160636955 1105 62-63 0.020200240416926363 1105 64-65 0.032830431328775944 1105 66-67 -0.32930796787722016 1105 68-69 0.8191621465661783 1105 70-71 0.5875007723120618 1105 72-73 0.58639959194997 1105 74-75 -1.6183856544437845 1105 76 -1.242077068477471 1106 1 -0.5164090129803398 1106 2 -0.7464228194175924 1106 3 -0.4232302322326298 1106 4 -0.10405684756379685 1106 5 -0.8197071352724237 1106 6 -0.06164708517162154 1106 7 0.10788330858434847 1106 8 -0.5700298311857566 1106 9 0.02784186808029432 1106 10-11 -0.052221101148234084 1106 12-13 -0.07271167534864276 1106 14-15 -0.30680461228570977 1106 16-17 0.1932834100772851 1106 18-19 -1.0271795440629035 1106 20-21 -1.1000484678464417 1106 22-23 -0.4327107638949883 1106 24-25 0.4353934036487672 1106 26-27 0.33065788806064944 1106 28-29 -1.1577877729943822 1106 30-31 -0.9816460040792023 1106 32-33 0.6328397132295436 1106 34-35 0.4685270747081063 1106 36-37 -0.5201920887291536 1106 38-39 -0.6246827209057102 1106 40-41 -0.5939404975386822 1106 42-43 0.1272813811153668 1106 44-45 0.40924963276847137 1106 46-47 0.14570989050172756 1106 48-49 -0.5041587877093008 1106 50-51 1.0122697248955603 1106 52-53 -0.5961802908742868 1106 54-55 -0.01900329247040844 1106 56-57 0.31474464967614324 1106 58-59 -0.4134381635476032 1106 60-61 -0.1278259916661426 1106 62-63 -0.14727287786264043 1106 64-65 -0.09259199570655596 1106 66-67 0.7965752886511908 1106 68-69 1.3581406411898271 1106 70-71 -0.6045499189321788 1106 72-73 -0.5349905770208494 1106 74-75 -1.6812120753347202 1106 76 -0.442998727463646 1107 1 -0.30184318664980836 1107 2 -0.07807548048201696 1107 3 0.1095428770110658 1107 4 0.8046266258255592 1107 5 0.12202955940544769 1107 6 -0.124392183210837 1107 7 0.3347740648868722 1107 8 1.6355724097105977 1107 9 -0.1654354428440712 1107 10-11 -0.3665068154339437 1107 12-13 -0.3472214792702104 1107 14-15 -0.6827149764313631 1107 16-17 0.5826391523741989 1107 18-19 0.9111958060771528 1107 20-21 0.20975545372218818 1107 22-23 -0.3427947975084322 1107 24-25 -0.560124803634146 1107 26-27 0.6676326779766129 1107 28-29 -1.1471435152913045 1107 30-31 0.11779377183115969 1107 32-33 1.045164643201531 1107 34-35 0.39093603829353896 1107 36-37 -0.8888195397095373 1107 38-39 0.4447850662091355 1107 40-41 1.0662835920971716 1107 42-43 0.4880656948408557 1107 44-45 0.5798378680625902 1107 46-47 1.089127257448503 1107 48-49 0.4207711842794879 1107 50-51 1.4187123019263765 1107 52-53 1.0085816138876211 1107 54-55 0.8364588924035345 1107 56-57 0.34471663847165956 1107 58-59 0.7787186991974906 1107 60-61 -0.2617195490891078 1107 62-63 0.0748559736779768 1107 64-65 -0.3368496987877876 1107 66-67 0.8584800505559613 1107 68-69 1.6718661313859116 1107 70-71 0.2030131062779077 1107 72-73 0.7585668459483372 1107 74-75 -0.40166025460642985 1107 76 -0.4161079711611251 1108 1 -1.8091408425542745 1108 2 -0.5642173056331288 1108 3 -0.6064382522827572 1108 4 0.33905092185976216 1108 5 0.2552302080859725 1108 6 0.09800203763539628 1108 7 0.589086316103149 1108 8 0.607413777836797 1108 9 -0.8982232948269697 1108 10-11 0.5037939364457529 1108 12-13 0.8846818083606323 1108 14-15 0.7072304754335903 1108 16-17 -0.9903005498224644 1108 18-19 0.41329664641328634 1108 20-21 -0.06947202674368214 1108 22-23 -0.08822454835613769 1108 24-25 -1.0139800299351975 1108 26-27 -1.3132017610621602 1108 28-29 0.42328992124120646 1108 30-31 0.5302587578255569 1108 32-33 -0.013400888274219369 1108 34-35 0.6761711849837937 1108 36-37 0.13281543006784347 1108 38-39 0.9845904620516848 1108 40-41 -0.5396798459096104 1108 42-43 -0.17447300484954553 1108 44-45 -0.03987317424906678 1108 46-47 0.3922011185719043 1108 48-49 0.4724076283307994 1108 50-51 -0.265424510693407 1108 52-53 0.05181469659439131 1108 54-55 0.03688643184036522 1108 56-57 0.40973211834781864 1108 58-59 -1.4417589655526157 1108 60-61 0.6457329055769634 1108 62-63 -0.12446586031878581 1108 64-65 0.809287703541564 1108 66-67 -0.9372592978149683 1108 68-69 0.7616494131196561 1108 70-71 1.3242721362056642 1108 72-73 0.9282926309992021 1108 74-75 1.5928480750412177 1108 76 1.3595075382005177 1109 1 -1.8133327382226483 1109 2 -0.9674931944702685 1109 3 -1.169627198098624 1109 4 -1.4421942264091392 1109 5 -0.9344142570591885 1109 6 -0.49763528576158933 1109 7 -2.136111634525637 1109 8 0.23511134032709435 1109 9 0.1049594407730865 1109 10-11 0.5570077231248405 1109 12-13 0.3733481645165142 1109 14-15 -0.6796659692178579 1109 16-17 -0.9295568764798219 1109 18-19 -1.1198891942946787 1109 20-21 0.9991508579015616 1109 22-23 0.37424245987239857 1109 24-25 -0.4051459979525802 1109 26-27 0.5802154101845431 1109 28-29 -0.454795779736898 1109 30-31 0.29943279912349396 1109 32-33 1.3822286723530084 1109 34-35 0.02739770260527763 1109 36-37 -0.8121853462091266 1109 38-39 0.3425317755123274 1109 40-41 0.6680906866163951 1109 42-43 0.030231233622743048 1109 44-45 -0.43942293360320406 1109 46-47 -0.07228420979325989 1109 48-49 0.12576114486549272 1109 50-51 0.24800508098490326 1109 52-53 0.6476460892352804 1109 54-55 -0.002315555428694438 1109 56-57 0.2278504229589089 1109 58-59 -0.07846134096016044 1109 60-61 0.2182759889491166 1109 62-63 -0.6337035563287188 1109 64-65 0.28254496173128985 1109 66-67 0.1517796712892121 1109 68-69 1.1487011131662328 1109 70-71 -0.09709100616353084 1109 72-73 -1.0979067169281365 1109 74-75 1.017355139161701 1109 76 -1.2966017615976533 1110 1 0.9121624155910872 1110 2 0.5678628948681244 1110 3 -0.17561118461358305 1110 4 -0.1516758951828443 1110 5 0.6564833409180544 1110 6 0.5574005338759989 1110 7 0.5840737847748301 1110 8 2.829970168814242 1110 9 -0.32453908430066036 1110 10-11 -0.49507824400537714 1110 12-13 0.45109784846088985 1110 14-15 -0.17823318371428343 1110 16-17 0.15995007674394657 1110 18-19 0.8966299797466206 1110 20-21 0.0713801035821362 1110 22-23 0.7006225694383517 1110 24-25 0.36872673698210434 1110 26-27 -0.8550563976536409 1110 28-29 0.5279265127199011 1110 30-31 0.08025875782556113 1110 32-33 1.2947444751343014 1110 34-35 0.6494794556604901 1110 36-37 0.008379339842278455 1110 38-39 0.5181744219514357 1110 40-41 0.3441547405565544 1110 42-43 0.5082337620677464 1110 44-45 1.347344870863715 1110 46-47 0.8647575095493423 1110 48-49 0.31012692657641594 1110 50-51 -0.15439694177110397 1110 52-53 -0.4723707670647599 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48-49 0.9641124338227911 2116 50-51 0.597466412266165 2116 52-53 1.0326499368689923 2116 54-55 0.9558414280264813 2116 56-57 0.9406245668604072 2116 58-59 0.608870325065233 2116 60-61 -0.7635920372147993 2116 62-63 1.3487416148909759 2116 64-65 1.1627392672334054 2116 66-67 0.2350328455870141 2116 68-69 0.829155133943452 2116 70-71 -1.9693014717272064 2116 72-73 0.9051232946147678 2116 74-75 0.7268106989924021 2116 76 0.26072798061088776 2117 1 0.6418160952447671 2117 2 1.0855035875088141 2117 3 2.0793671703647725 2117 4 0.6311163126093611 2117 5 0.5486911331258462 2117 6 0.18911049058595353 2117 7 -0.8612725355714943 2117 8 -0.19741078356670982 2117 9 1.5034912187296428 2117 10-11 1.399726950799817 2117 12-13 1.2562926536556915 2117 14-15 0.24471053922944463 2117 16-17 0.23089163518550748 2117 18-19 0.6632966464132863 2117 20-21 1.0965965537985838 2117 22-23 0.7723217036374876 2117 24-25 0.6139107196660802 2117 26-27 0.986339706242461 2117 28-29 0.23220140449479487 2117 30-31 0.32381936388616595 2117 32-33 1.2009133063031356 2117 34-35 0.9648474210284519 2117 36-37 -0.00823321427027679 2117 38-39 1.2758584046354144 2117 40-41 -0.8927500213482062 2117 42-43 -1.5901428613088768 2117 44-45 1.0694769054957476 2117 46-47 1.0438159511077956 2117 48-49 -1.021312467362982 2117 50-51 0.4443043569302034 2117 52-53 1.00127641908243 2117 54-55 0.5689837205166057 2117 56-57 0.30327278820428205 2117 58-59 -0.02848145359089216 2117 60-61 1.4824553936152398 2117 62-63 0.8568937888040224 2117 64-65 0.8937175281029681 2117 66-67 0.03542810250400663 2117 68-69 0.5134436714928583 2117 70-71 0.7394435875613254 2117 72-73 0.7410916740614013 2117 74-75 -0.0071319887546295035 2117 76 -0.2615052209701396 2118 1 -1.1971587596875324 2118 2 0.12297940955910391 2118 3 -0.668215034664243 2118 4 -0.12492817481810192 2118 5 0.21696966310650367 2118 6 -0.13753360999431408 2118 7 0.1960292052408832 2118 8 -4.56709163463054 2118 9 -2.077021353804202 2118 10-11 -1.7965980008442806 2118 12-13 -0.05802069257255482 2118 14-15 -1.2224074491651393 2118 16-17 -1.1415696800949604 2118 18-19 -2.44840548124629 2118 20-21 -2.6270494810176643 2118 22-23 -1.1076347760530396 2118 24-25 -0.4398852184383628 2118 26-27 -0.4767889204499909 2118 28-29 -1.5126003363175826 2118 30-31 -0.8598121641602532 2118 32-33 -0.2993284731939667 2118 34-35 -0.16668062539321227 2118 36-37 -0.7469904676551877 2118 38-39 -1.1117141292137092 2118 40-41 -2.209239383050331 2118 42-43 -1.8805200373243522 2118 44-45 -0.6886227076570606 2118 46-47 -0.9773397548883409 2118 48-49 -1.069305697537061 2118 50-51 -0.6872236894914607 2118 52-53 -2.090962459060705 2118 54-55 -1.1769060280327253 2118 56-57 -0.7238065154707698 2118 58-59 -0.2603479913084996 2118 60-61 -2.4186567920714097 2118 62-63 -0.7189040835364011 2118 64-65 -0.8496335357268237 2118 66-67 -3.615684083182643 2118 68-69 -0.10817141554775844 2118 70-71 -1.4978629888409927 2118 72-73 -3.020755521296433 2118 74-75 -1.0519820854664346 2118 76 -1.6065245633299057 2119 1 1.120239570503685 2119 2 0.018616360811460453 2119 3 0.5528999666823786 2119 4 0.556461537005223 2119 5 0.2293585669578384 2119 6 1.4303360371912461 2119 7 -0.8179756425910583 2119 8 -0.7907652139464574 2119 9 -0.004707860671963715 2119 10-11 0.06025629487346862 2119 12-13 0.22867470198710294 2119 14-15 0.5277945438324352 2119 16-17 0.03806942574937011 2119 18-19 -0.16581727763734477 2119 20-21 -1.020060523301531 2119 22-23 0.7416111167560047 2119 24-25 1.3776477737512351 2119 26-27 0.7243287741365947 2119 28-29 1.1026703342991624 2119 30-31 0.5059971544500286 2119 32-33 0.9829904064182102 2119 34-35 0.3166886178063564 2119 36-37 -1.8732360911402388 2119 38-39 -0.33619568051993554 2119 40-41 -0.8785095150190898 2119 42-43 0.03572019968076745 2119 44-45 -0.7578389748264627 2119 46-47 0.20182803396164672 2119 48-49 -0.28691948692569724 2119 50-51 1.1727278321891106 2119 52-53 0.40008251804675155 2119 54-55 -0.6662003991611911 2119 56-57 1.5319839504597468 2119 58-59 1.6321917339810241 2119 60-61 0.834440433891416 2119 62-63 0.2548368267787069 2119 64-65 -0.33333943392234744 2119 66-67 1.216527066830821 2119 68-69 -0.20936990733337524 2119 70-71 -0.22624973810034987 2119 72-73 -1.4402949772619564 2119 74-75 -0.12328561361078982 2119 76 0.5758077824821015 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 3.0 13 59.0 14 198.0 15 373.0 16 529.0 17 569.0 18 532.0 19 524.0 20 459.0 21 372.0 22 360.0 23 328.0 24 353.0 25 383.0 26 500.0 27 567.0 28 668.0 29 892.0 30 1066.0 31 1231.0 32 1739.0 33 2236.0 34 3159.0 35 4924.0 36 8171.0 37 8944.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.37403559304598 22.35366731817714 10.261289990741693 33.011007098035186 2 16.72159628011548 22.771518356710356 35.66592575560154 24.84095960757262 3 15.720088909327815 25.308500038322983 28.908305868526607 30.063105183822593 4 12.028307912419201 19.26113282746992 39.623412789657905 29.08714647045298 5 12.14838660228405 36.925474566311536 36.00572289926164 14.920415932142767 6 24.30188293605171 37.801793515750745 20.96011854577042 16.936205002427123 7 25.48478577450755 30.374798804322833 23.43833831532153 20.702077105848087 8 21.412329782069953 34.035921412329785 21.821108300758794 22.73064050484147 9 22.63355560665287 15.262767941544672 23.172632278173783 38.93104417362868 10-11 23.373189238905496 24.93165734140671 27.946398916736925 23.74875450295087 12-13 21.892644541529343 22.659104264070923 27.03303441404154 28.415216780358193 14-15 19.459134922459825 25.107943077591273 24.883114892312406 30.549807107636497 16-17 21.810888837791573 27.75861628471424 26.195038450729413 24.235456426764774 18-19 21.463427096906056 26.652359418512557 26.146496001635118 25.73771748294627 20-21 20.5155719066963 27.45458726143941 26.06857259651005 25.961268235354236 22-23 22.370404435246925 26.62042359673999 26.423698934620987 24.585473033392095 24-25 21.3382386755576 28.265757134462582 25.873125367261952 24.522878822717868 26-27 19.95094657775734 28.394777854423754 26.929562351498426 24.72471321632048 28-29 21.413607214940853 27.075189698781326 28.199330625175644 23.31187246110217 30-31 21.53751820341841 27.079021997394037 27.20293298587159 24.18052681331596 32-33 19.749112184154725 29.08714647045298 26.938504381594747 24.225236963797553 34-35 21.296083390817813 27.971947574154974 27.008763189494395 23.723205845532817 36-37 22.252880611123885 27.034311846912445 26.69579213612325 24.01701540584042 38-39 21.121075087504153 27.542730129531694 27.814823331033956 23.5213714519302 40-41 21.357400168621137 26.25252292992003 28.11374262282517 24.27633427863366 42-43 20.630540865077542 26.599984670805547 27.92340512506068 24.846069339056232 44-45 19.968830637949974 26.685572673156027 27.92979228941519 25.41580439947881 46-47 20.607547073401292 26.84269691627705 28.08436166679441 24.465394343527247 48-49 19.52811629748857 27.472471321632046 28.323241613653206 24.676170767226182 50-51 20.06208323752587 26.98321453207634 28.2248792825937 24.72982294780409 52-53 19.676298510513273 26.955111008916482 28.284918627526125 25.08367185304412 54-55 18.92005825093891 27.45841956005212 27.920850259318875 25.700671929690095 56-57 19.654582151707928 26.70217930047776 27.84675915280652 25.796479395007793 58-59 19.84491964947242 26.928284918627526 27.699854372652716 25.52694105924734 60-61 19.363327457142127 26.767328376893794 27.316624511381924 26.55271965458215 62-63 19.76699624434736 26.44797015916814 27.81226846529215 25.972765131192354 64-65 20.05314120742955 27.2042104187425 27.12756444648834 25.615083927339615 66-67 20.198768554712448 27.12884187935924 26.46968651797348 26.202703047954827 68-69 19.929230218951993 27.30895991415651 26.271684422983572 26.490125443907925 70-71 20.81960092997113 27.182494059937152 25.907616054776323 26.0902889553154 72-73 20.71357400168621 26.527170997164102 25.521831327763728 27.237423673385962 74-75 20.54750772846887 26.98193709920544 25.701949362561 26.768605809764694 76 21.156843207889427 26.601262103676454 25.06834265859329 27.17355202984083 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 0.5 14 0.0 15 0.0 16 2.5 17 5.0 18 4.5 19 8.5 20 11.0 21 9.0 22 13.0 23 20.5 24 34.0 25 44.0 26 66.0 27 102.0 28 155.5 29 195.0 30 235.5 31 319.5 32 446.5 33 530.0 34 610.5 35 827.0 36 1074.0 37 1185.0 38 1330.5 39 1690.5 40 2098.0 41 2439.0 42 2587.0 43 2637.5 44 2781.5 45 2879.5 46 2884.0 47 2983.5 48 2870.0 49 2512.5 50 2368.0 51 2226.5 52 1909.5 53 1549.5 54 1365.0 55 1253.0 56 1031.0 57 827.0 58 733.0 59 633.5 60 442.0 61 303.5 62 257.0 63 217.0 64 158.0 65 116.0 66 84.5 67 76.0 68 60.0 69 42.0 70 32.0 71 24.0 72 19.5 73 17.0 74 14.5 75 10.0 76 10.0 77 6.5 78 3.0 79 3.0 80 4.0 81 4.0 82 3.0 83 3.0 84 2.5 85 1.5 86 1.5 87 2.0 88 1.0 89 1.5 90 1.5 91 1.0 92 2.0 93 1.0 94 0.5 95 1.0 96 1.0 97 1.5 98 1.0 99 2.5 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.656600495643954 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 39141.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.78025599754733 #Duplication Level Percentage of deduplicated Percentage of total 1 75.1870533837747 45.69888352367083 2 11.09289617486339 13.484581385248203 3 5.666246321984027 10.331877059860505 4 2.87515762925599 6.990112669579214 5 1.7612442202606136 5.352443729082037 6 1.0046237915090375 3.6636774737487547 7 0.7313997477931904 3.111826473518817 8 0.4287515762925599 2.084770445313099 9 0.344682639764607 1.8854909174522878 >10 0.9079445145018915 7.396336322526251 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.010219462967221073 0.0 2 0.0 0.0 0.0 0.010219462967221073 0.0 3 0.0 0.0 0.0 0.010219462967221073 0.0 4 0.0 0.0 0.0 0.010219462967221073 0.0 5 0.0 0.0 0.0 0.010219462967221073 0.0 6 0.0 0.0 0.0 0.010219462967221073 0.0 7 0.0 0.0 0.0 0.01277432870902634 0.0 8 0.0 0.0 0.0 0.015329194450831609 0.0 9 0.0 0.0 0.0 0.017884060192636875 0.0 10 0.0 0.0 0.0 0.022993791676247412 0.0 11 0.0 0.0 0.0 0.03576812038527375 0.0 12 0.0 0.0 0.0 0.0485424490943001 0.0 13 0.0 0.0 0.0 0.0485424490943001 0.0 14 0.0 0.0 0.0 0.061316777803326436 0.0 15 0.0 0.0 0.0 0.06642650928693697 0.0 16 0.0 0.0 0.0 0.0715362407705475 0.0 17 0.0 0.0 0.0 0.08175570373776858 0.0 18 0.0 0.0 0.0 0.08175570373776858 0.0 19 0.0 0.0 0.0 0.08686543522137911 0.0 20 0.0 0.0 0.0 0.08686543522137911 0.0 21 0.0 0.0 0.0 0.08686543522137911 0.0 22 0.0 0.0 0.0 0.09453003244679492 0.0 23 0.0 0.0 0.0 0.09453003244679492 0.0 24 0.0 0.0 0.0 0.09963976393040545 0.0 25 0.0 0.0 0.0 0.10219462967221073 0.0 26 0.0 0.0 0.0 0.10730436115582126 0.0 27 0.0 0.0 0.0 0.12774328709026342 0.0 28 0.0 0.0 0.0 0.15073707876651082 0.0 29 0.0 0.0 0.0 0.16351140747553716 0.0 30 0.0 0.0 0.0 0.17884060192636878 0.0 31 0.0 0.0 0.0 0.21460872231164252 0.0 32 0.0 0.0 0.0 0.25293170843872154 0.0 33 0.0 0.0 0.0 0.28614496308219 0.0 34 0.0 0.0 0.0 0.3423520094019059 0.0 35 0.0 0.0 0.0 0.4011139214634271 0.0 36 0.0 0.0 0.0 0.5160828798446642 0.0 37 0.0 0.0 0.0 0.6923686160292276 0.0 38 0.0 0.0 0.0 1.019391430980302 0.0 39 0.0 0.0 0.0 1.4051761579928974 0.0 40 0.0 0.0 0.0 1.888045783194093 0.0 41 0.0 0.0 0.0 2.350476482460847 0.0 42 0.0 0.0 0.0 2.8614496308219004 0.0 43 0.0 0.0 0.0 3.400526302342812 0.0 44 0.0 0.0 0.0 3.993255154441634 0.0 45 0.0 0.0 0.0 4.550215886155183 0.0 46 0.0 0.0 0.0 5.135280141028589 0.0 47 0.0 0.0 0.0 5.692240872742137 0.0 48 0.0 0.0 0.0 6.24664673871388 0.0 49 0.0 0.0 0.0 6.8495950537799235 0.0 50 0.0 0.0 0.0 7.375897396591809 0.0 51 0.0 0.0 0.0 7.920083799596331 0.0 52 0.0 0.0 0.0 8.477044531309879 0.0 53 0.0 0.0 0.0 8.962469022252881 0.0 54 0.0 0.0 0.0 9.570527068802534 0.0 55 0.0 0.0 0.0 10.153036457934135 0.0 56 0.0 0.0 0.0 10.656345009069774 0.0 57 0.0 0.0 0.0 11.157098694463606 0.0 58 0.0 0.0 0.0 11.553102884443422 0.0 59 0.0 0.0 0.0 11.989984926292124 0.0 60 0.0 0.0 0.0 12.490738611685956 0.0 61 0.0 0.0 0.0 12.942949847985489 0.0 62 0.0 0.0 0.0 13.331289440739889 0.0 63 0.0 0.0 0.0 13.832043126133721 0.0 64 0.0 0.0 0.0 14.29191895965867 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGCAGG 15 0.0022096222 69.99361 39 CAGCCGG 15 0.0022096222 69.99361 24 GCCGGAA 15 0.0022096222 69.99361 26 GTTAACA 15 0.0022096222 69.99361 57 CGCAGGA 15 0.0022096222 69.99361 40 GCCTATT 15 0.0022096222 69.99361 33 TTAAACG 15 0.0022096222 69.99361 12 CCTGGCA 15 0.0022096222 69.99361 19 AGGATTT 15 0.0022096222 69.99361 43 CAATACG 15 0.0022096222 69.99361 69 CTGGCAG 15 0.0022096222 69.99361 20 TTTCAAC 25 2.363553E-4 55.99489 42 CCCCGAA 25 2.363553E-4 55.99489 13 AACAACT 20 0.0069046635 52.495205 55 AGCCGGA 20 0.0069046635 52.495205 25 GGCAGCC 20 0.0069046635 52.495205 22 CAAGTAG 20 0.0069046635 52.495205 31 GCAGTGC 20 0.0069046635 52.495205 52 CCGGAAG 20 0.0069046635 52.495205 27 TTGTATT 20 0.0069046635 52.495205 37 >>END_MODULE