FastQCFastQC Report
Wed 25 May 2016
SRR1780572_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780572_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39141
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT5391.3770726348330395TruSeq Adapter, Index 5 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT4301.0985922689762653TruSeq Adapter, Index 5 (96% over 26bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC1270.3244679492092691No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC480.12263355560665286RNA PCR Primer, Index 5 (95% over 21bp)
TCTGTACCCACGCCTTCTTCAAAGCCATACTATTTATGTGCTCCGGATCC420.10730436115582126No Hit
CTTTACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC400.10219462967221073RNA PCR Primer, Index 5 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCGG150.00220882270.033
GAGGCAC150.00220882270.07
TTGTCGT150.00220882270.03
ATTAGAG150.00220882270.03
ATAGGGA150.00220882270.041
TGTCAGG150.00220882270.029
TGTTATA150.00220882270.024
TTATAAT150.00220882270.026
AGTACTG150.00220882270.030
GACACAT150.00220882270.026
AGTACAA150.00220882270.049
GCAGATG150.00220882270.012
TACTGCG150.00220882270.032
AGATGGA150.00220882270.014
CTTGTTA150.00220882270.022
GTGACAC150.00220882270.024
GTAGGCA150.00220882270.08
TCGTAGG150.00220882270.06
GTTATAA150.00220882270.025
CAGTGAC150.00220882270.022
Produced by FastQC (version 0.11.5)