##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780572_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 39141 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.670652257223885 34.0 34.0 34.0 31.0 34.0 2 33.22927365166961 34.0 34.0 34.0 32.0 34.0 3 33.43992233208145 34.0 34.0 34.0 34.0 34.0 4 33.48123451112644 34.0 34.0 34.0 34.0 34.0 5 33.464474591860196 34.0 34.0 34.0 34.0 34.0 6 37.18410362535449 38.0 37.0 38.0 36.0 38.0 7 36.76469686517974 38.0 37.0 38.0 34.0 38.0 8 36.7690401369408 38.0 37.0 38.0 34.0 38.0 9 36.60836463043867 38.0 37.0 38.0 34.0 38.0 10-11 36.24453897447689 38.0 37.0 38.0 33.0 38.0 12-13 36.148923124089826 38.0 37.0 38.0 33.5 38.0 14-15 36.429485705526176 38.0 37.0 38.0 34.0 38.0 16-17 36.53004522112363 38.0 37.0 38.0 34.0 38.0 18-19 36.531501494596455 38.0 37.0 38.0 34.0 38.0 20-21 36.222452160138985 38.0 37.0 38.0 33.5 38.0 22-23 36.321925857796174 38.0 37.0 38.0 33.5 38.0 24-25 35.60639738381748 38.0 36.0 38.0 30.0 38.0 26-27 35.83160879895762 38.0 36.5 38.0 32.0 38.0 28-29 35.66607904754605 38.0 36.0 38.0 31.0 38.0 30-31 35.34138116042001 38.0 36.0 38.0 29.0 38.0 32-33 35.88954037965305 38.0 36.5 38.0 32.5 38.0 34-35 35.88478832937329 38.0 36.5 38.0 32.5 38.0 36-37 36.06949234817711 38.0 37.0 38.0 34.0 38.0 38-39 35.63365780128254 38.0 36.5 38.0 30.5 38.0 40-41 35.1583633530058 37.0 35.5 38.0 29.0 38.0 42-43 35.65071153010909 38.0 36.5 38.0 32.5 38.0 44-45 35.89077948953783 38.0 36.5 38.0 32.5 38.0 46-47 36.14611277177384 38.0 37.0 38.0 34.0 38.0 48-49 35.87893768682456 38.0 36.5 38.0 32.0 38.0 50-51 36.054814644490435 38.0 37.0 38.0 33.5 38.0 52-53 35.98023811348713 38.0 37.0 38.0 32.5 38.0 54-55 36.17783142995836 38.0 37.0 38.0 34.0 38.0 56-57 36.24091106512353 38.0 37.0 38.0 34.0 38.0 58-59 36.26404537441557 38.0 37.0 38.0 34.0 38.0 60-61 35.91333895403797 38.0 37.0 38.0 32.5 38.0 62-63 36.2360568202141 38.0 37.0 38.0 34.0 38.0 64-65 36.245880278991336 38.0 37.0 38.0 34.0 38.0 66-67 35.57083365269155 38.0 36.0 38.0 31.0 38.0 68-69 34.85285250760073 37.5 35.5 38.0 26.0 38.0 70-71 34.79453769704402 37.0 35.0 38.0 26.0 38.0 72-73 34.455813596995476 37.0 34.5 38.0 24.5 38.0 74-75 35.12173935259702 38.0 36.0 38.0 27.5 38.0 76 34.57502363250811 37.0 35.0 38.0 25.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5222420297331638 1101 2 0.2130713454427351 1101 3 0.36088055640563255 1101 4 0.3834120390463056 1101 5 0.09824762655884456 1101 6 -0.6410887275484356 1101 7 0.08339464903511384 1101 8 0.5787872191963928 1101 9 0.5327650745196735 1101 10-11 0.3412549728267109 1101 12-13 0.6952375045320878 1101 14-15 0.4657933270334951 1101 16-17 0.011892028603213589 1101 18-19 0.004361838737935386 1101 20-21 0.14147635947754367 1101 22-23 0.7256604103772588 1101 24-25 -2.5677964743531305 1101 26-27 -0.3640254765711859 1101 28-29 0.14431757947463808 1101 30-31 1.535092681518634 1101 32-33 0.6989736513393296 1101 34-35 0.815065105206692 1101 36-37 0.43632232275204785 1101 38-39 0.7273324740310443 1101 40-41 1.3414864751419913 1101 42-43 0.326371508912608 1101 44-45 0.17539622485008977 1101 46-47 1.049311300512393 1101 48-49 -2.124147001457409 1101 50-51 -0.9575468057554204 1101 52-53 -0.7919986617358958 1101 54-55 -0.33480745340779094 1101 56-57 -0.8878579259489783 1101 58-59 -0.5814265131052991 1101 60-61 -2.2053848893705776 1101 62-63 -0.061294753053033446 1101 64-65 -0.7689533329760181 1101 66-67 -0.9618458974530881 1101 68-69 -1.8728576054861463 1101 70-71 -1.7802307742396266 1101 72-73 -1.5870274857976483 1101 74-75 -1.325807854203724 1101 76 -1.4654381436489885 1102 1 -0.5639279761022777 1102 2 0.5271153057617397 1102 3 0.24718672252117813 1102 4 0.4772653749731717 1102 5 0.11526766157148671 1102 6 0.0681118170926851 1102 7 -0.008999826747803752 1102 8 0.4461282034407006 1102 9 0.4064277860543939 1102 10-11 0.6803426989500352 1102 12-13 0.7302015193152016 1102 14-15 0.330411105675779 1102 16-17 0.3442689980644147 1102 18-19 -0.23576654095472804 1102 20-21 0.6992472211776075 1102 22-23 0.6990118984146108 1102 24-25 -4.2161139223204565 1102 26-27 -0.8102907946027003 1102 28-29 0.23345393426942707 1102 30-31 1.659679337811184 1102 32-33 0.23461846752294946 1102 34-35 0.5904492717112646 1102 36-37 -2.175766764974078 1102 38-39 0.16751920812946963 1102 40-41 1.2177265062643343 1102 42-43 0.8227243585527546 1102 44-45 0.8138420525100827 1102 46-47 1.1450125259549182 1102 48-49 -2.7443084486466773 1102 50-51 -0.5318027870820075 1102 52-53 0.24719604746464796 1102 54-55 0.5458091581463691 1102 56-57 0.841815289378772 1102 58-59 -0.40500169303138733 1102 60-61 -5.323601189701257 1102 62-63 -2.4502268674276664 1102 64-65 -0.28004067006997246 1102 66-67 0.5950010194891391 1102 68-69 -1.8012275724186466 1102 70-71 -2.1473772437980827 1102 72-73 -5.70966899523161 1102 74-75 -2.413582606197501 1102 76 -1.3945375406534595 1103 1 1.033096107346104 1103 2 0.6380985854058565 1103 3 0.09770060501849542 1103 4 0.2990938762770483 1103 5 0.4314971446210407 1103 6 0.6114466844036244 1103 7 0.9653827472666165 1103 8 0.6938655864749066 1103 9 0.15048362283917527 1103 10-11 0.3850903596341908 1103 12-13 -0.2439126086185297 1103 14-15 -0.05553765947225031 1103 16-17 -0.03144046657740773 1103 18-19 0.5786221689700994 1103 20-21 0.08611637288799301 1103 22-23 0.7627486678577711 1103 24-25 -2.6303645742609376 1103 26-27 -0.36394166130004635 1103 28-29 0.03154413215253982 1103 30-31 0.6447230561728929 1103 32-33 1.0469746571225755 1103 34-35 0.4026520635505051 1103 36-37 -0.7388515939355784 1103 38-39 -0.013720245774507589 1103 40-41 -0.26789245369884895 1103 42-43 -0.05398219153160966 1103 44-45 0.9929722872086586 1103 46-47 0.5667029692744379 1103 48-49 -1.3347747778382697 1103 50-51 -0.27114164273471175 1103 52-53 0.12355745258813045 1103 54-55 -0.21377820187817065 1103 56-57 0.4242282361497516 1103 58-59 -0.03063445879300275 1103 60-61 -4.607195299227257 1103 62-63 -1.333107677367849 1103 64-65 0.07607223068168167 1103 66-67 -0.9237518567188729 1103 68-69 -0.11458084746082875 1103 70-71 -0.7209314699063754 1103 72-73 -4.8756453719765105 1103 74-75 -3.0289760714529095 1103 76 -3.5734760281515427 1104 1 0.15800998360350604 1104 2 0.5040900419305956 1104 3 0.2383923509189998 1104 4 -0.23060159016913673 1104 5 -0.6655126390361232 1104 6 -0.13479832041930706 1104 7 0.15809323369351347 1104 8 0.3656996776971795 1104 9 0.48829539078736417 1104 10-11 -1.4197532396492676 1104 12-13 0.18095303834303422 1104 14-15 0.44897522463963924 1104 16-17 0.34274997024221676 1104 18-19 0.4968046135064128 1104 20-21 -0.07641635413418868 1104 22-23 0.5200864107267336 1104 24-25 0.3487245810373949 1104 26-27 1.0226161449559825 1104 28-29 1.4455581875473271 1104 30-31 1.2157234695668677 1104 32-33 0.8061726728315506 1104 34-35 -0.08088107686564427 1104 36-37 0.9188926529984158 1104 38-39 1.262978114429437 1104 40-41 2.019933094048156 1104 42-43 0.6831336965764265 1104 44-45 1.1266018862165055 1104 46-47 0.9490579368919114 1104 48-49 -0.6104741026281246 1104 50-51 0.4418940469442134 1104 52-53 0.5712286665884108 1104 54-55 0.10704996403060818 1104 56-57 0.32094864403179457 1104 58-59 -0.6071123422159062 1104 60-61 0.27705439072727245 1104 62-63 0.08958765128035395 1104 64-65 0.8179765988778982 1104 66-67 0.35787278156141156 1104 68-69 2.302705910152511 1104 70-71 1.8047334053864432 1104 72-73 0.9680572599650645 1104 74-75 0.08625749613700151 1104 76 -0.04564083454537382 1105 1 0.7206124986390492 1105 2 0.010377630776957858 1105 3 0.3676979824116202 1105 4 -0.7549318252702832 1105 5 -0.20487841268292328 1105 6 -0.08039700822685347 1105 7 0.6932217192047219 1105 8 -1.0759350954028761 1105 9 0.41844847990709866 1105 10-11 0.4769799478849137 1105 12-13 -0.9489676833628238 1105 14-15 -0.3249449503193489 1105 16-17 -0.6599154206817843 1105 18-19 0.12645196178416285 1105 20-21 0.07592098867608144 1105 22-23 -0.15719625739792775 1105 24-25 0.4752281348919638 1105 26-27 0.18103604654154992 1105 28-29 0.36484468289997807 1105 30-31 1.13685523555921 1105 32-33 0.616998260584424 1105 34-35 -1.7649980806928696 1105 36-37 -3.254989972134581E-4 1105 38-39 -0.8212209014262228 1105 40-41 0.46361816785898213 1105 42-43 -0.1789494089457193 1105 44-45 0.5183460086878 1105 46-47 0.18183541092144395 1105 48-49 0.32576154526689294 1105 50-51 0.42863543568122253 1105 52-53 0.13512970540743652 1105 54-55 0.36709643751338206 1105 56-57 0.1245298359399385 1105 58-59 0.056501654503613 1105 60-61 1.0196733081684997 1105 62-63 -1.740169046368635 1105 64-65 0.40019365737981616 1105 66-67 0.14764861535037 1105 68-69 -0.45125800455497966 1105 70-71 0.3225710215701483 1105 72-73 0.5903918690410563 1105 74-75 -0.49848826657486 1105 76 -0.017483371450786933 1106 1 -2.9053475628048844 1106 2 0.12450973523164066 1106 3 0.16785159224264845 1106 4 0.4475259320261813 1106 5 0.24473756273950187 1106 6 -0.13907596367999986 1106 7 -1.4587752085986594 1106 8 0.352866747915094 1106 9 -0.30758838645234476 1106 10-11 0.5079707312950461 1106 12-13 1.2816390754697409 1106 14-15 -0.3287083911795676 1106 16-17 -0.35757287075859523 1106 18-19 0.6857069541036722 1106 20-21 0.6361744448972857 1106 22-23 0.6848160313885501 1106 24-25 -0.09877340120635125 1106 26-27 0.8704369682005364 1106 28-29 0.6750981391211823 1106 30-31 0.38816091912296 1106 32-33 -0.5224794659900738 1106 34-35 0.6807008440383058 1106 36-37 0.7193365593745256 1106 38-39 -0.5104682132541782 1106 40-41 0.8489484289956906 1106 42-43 0.9738923729283115 1106 44-45 1.4535994649090043 1106 46-47 0.9383638287401794 1106 48-49 0.5955388110118989 1106 50-51 0.0964541760806128 1106 52-53 0.3204215640863879 1106 54-55 -0.13294196494436505 1106 56-57 0.18543613394301417 1106 58-59 0.47900549474939424 1106 60-61 0.9102263035602007 1106 62-63 0.30080637605840366 1106 64-65 -1.7233470492254028 1106 66-67 -0.5653314153715954 1106 68-69 0.3088802442929506 1106 70-71 -0.19805109824551437 1106 72-73 0.079639180869016 1106 74-75 -0.5484882665748572 1106 76 -0.5256246924953274 1107 1 0.9405908125452527 1107 2 0.05280105175825156 1107 3 0.2748543933630998 1107 4 0.07049511970124911 1107 5 -0.33621481821287347 1107 6 0.22619014276257587 1107 7 0.7972472003649287 1107 8 0.1881608655621534 1107 9 -0.25212620157839893 1107 10-11 0.737382496000933 1107 12-13 0.14018249283668638 1107 14-15 0.2018238217055881 1107 16-17 0.6637156446475743 1107 18-19 0.7406089148879857 1107 20-21 0.9185273860737553 1107 22-23 -0.08353130754701965 1107 24-25 0.013551528765646026 1107 26-27 -0.9463697544885434 1107 28-29 0.726358643322861 1107 30-31 0.841382207638361 1107 32-33 1.364355267903477 1107 34-35 0.353529975690968 1107 36-37 1.1703169515313903 1107 38-39 -0.5009444037303723 1107 40-41 0.48984478753911276 1107 42-43 1.0971416726482062 1107 44-45 0.798977616169509 1107 46-47 1.002789599048306 1107 48-49 0.8994603796393577 1107 50-51 1.1356698623551225 1107 52-53 1.2097773063833088 1107 54-55 0.6003913683889621 1107 56-57 0.8708703076124777 1107 58-59 0.5683612370463109 1107 60-61 1.3934195808711252 1107 62-63 0.9223750035093872 1107 64-65 1.0948602336877613 1107 66-67 0.34587306642112026 1107 68-69 1.1080399081584886 1107 70-71 0.42967999419145997 1107 72-73 0.8877624301687348 1107 74-75 0.09184786787891852 1107 76 1.3023865119864624 1108 1 -0.5920643547848314 1108 2 0.45859494826422065 1108 3 -0.1682386333212591 1108 4 0.4244682879159072 1108 5 0.05601575822822724 1108 6 -0.3701536579155871 1108 7 -0.46679525872396965 1108 8 -0.20878738742324998 1108 9 -0.1745056796854314 1108 10-11 0.4022063202674815 1108 12-13 1.004696719580025 1108 14-15 0.20901090706604464 1108 16-17 -0.5588260035906742 1108 18-19 -0.12206246945522281 1108 20-21 0.9139939937694592 1108 22-23 -0.5297203094635776 1108 24-25 0.7394471501720972 1108 26-27 -0.760390099468637 1108 28-29 -1.3672577506031303 1108 30-31 0.011218563233228451 1108 32-33 0.7079716618294682 1108 34-35 0.42543768614357447 1108 36-37 0.8076824240361802 1108 38-39 1.1456721376230163 1108 40-41 0.737670233506968 1108 42-43 -0.9665838175478783 1108 44-45 0.2665067330794244 1108 46-47 0.733476610695071 1108 48-49 0.7383959538690519 1108 50-51 0.12840906329865476 1108 52-53 -0.016044601327145358 1108 54-55 -0.7160246717112884 1108 56-57 -0.04539093372615355 1108 58-59 0.22161201104011496 1108 60-61 0.7034593862669709 1108 62-63 0.6912825665345963 1108 64-65 0.05221686054903074 1108 66-67 0.6448189605682515 1108 68-69 1.0409604447941945 1108 70-71 -0.5283769127818587 1108 72-73 -1.0036941524643197 1108 74-75 -1.3625233542941615 1108 76 0.0011923501111823498 1109 1 -0.48478287675347076 1109 2 0.3847710078822928 1109 3 0.34728690619123626 1109 4 -0.35584532798224444 1109 5 -0.293231253105418 1109 6 -0.5577021752265665 1109 7 -1.357805971346238 1109 8 -0.06230392212282965 1109 9 0.44007280191680564 1109 10-11 0.3590453204227373 1109 12-13 0.5770457337082604 1109 14-15 0.6751264170800226 1109 16-17 0.8473997377538396 1109 18-19 0.8811978938423977 1109 20-21 1.1448975801690935 1109 22-23 0.41524165296461746 1109 24-25 0.06047649344177586 1109 26-27 0.33276313760634935 1109 28-29 -0.7807804955185205 1109 30-31 0.4080513531726808 1109 32-33 0.39804307931122196 1109 34-35 -0.3682667075841053 1109 36-37 0.9180301961212649 1109 38-39 1.2411542056248805 1109 40-41 0.5367697522152426 1109 42-43 0.600525326994898 1109 44-45 1.0109951665524903 1109 46-47 0.8740991848295181 1109 48-49 0.6779239774678629 1109 50-51 0.07643731978057389 1109 52-53 -0.3662619588803153 1109 54-55 -0.5791282270598401 1109 56-57 -0.2206742748222581 1109 58-59 -0.48239357815410955 1109 60-61 1.0227421063414894 1109 62-63 0.7266807966230928 1109 64-65 0.7671291412507841 1109 66-67 0.9343314586275611 1109 68-69 -0.026265141294913974 1109 70-71 0.4211650838025207 1109 72-73 -0.14012483093038952 1109 74-75 -0.08919623117662923 1109 76 -0.07286447336343116 1110 1 -1.1148713723286932 1110 2 -0.42787121714930976 1110 3 0.2535658779569374 1110 4 -0.27628359178333994 1110 5 0.35902327702521575 1110 6 0.2514002267961928 1110 7 0.7317009818775304 1110 8 0.7052477002960487 1110 9 0.8066973278333691 1110 10-11 -0.29202926870495105 1110 12-13 0.6387819326126021 1110 14-15 0.7617677992966208 1110 16-17 0.5519509387652235 1110 18-19 -0.033340664943949605 1110 20-21 0.9314125401353763 1110 22-23 -0.3580411114685944 1110 24-25 -0.6654400678730212 1110 26-27 1.0799607777243452 1110 28-29 0.0750981391211738 1110 30-31 -1.054696223734183 1110 32-33 0.7489491054384914 1110 34-35 1.123557986895456 1110 36-37 1.2193365593745256 1110 38-39 1.0181032153172538 1110 40-41 -1.146289666242403 1110 42-43 -0.349917150881204 1110 44-45 0.8250280363375708 1110 46-47 0.6812209715973196 1110 48-49 1.5336340491071425 1110 50-51 -0.7273553477289099 1110 52-53 0.07756442122924767 1110 54-55 -0.46627529827770076 1110 56-57 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2116 48-49 0.02825102633281773 2116 50-51 -1.1944360930705287 2116 52-53 -0.16974406738358994 2116 54-55 0.08098143050076345 2116 56-57 -0.3742429550839006 2116 58-59 -2.269234670882078 2116 60-61 -0.9026618951975678 2116 62-63 0.0662825665345963 2116 64-65 -2.097001293531825 2116 66-67 -1.2983541896987205 2116 68-69 -1.4277657184399715 2116 70-71 0.8581600818787081 2116 72-73 0.9075377315936564 2116 74-75 0.5993378203816633 2116 76 -0.9456039885615866 2117 1 0.6942195367622119 2117 2 0.3688820296039381 2117 3 0.0887390381300932 2117 4 -0.29782038832014024 2117 5 0.293655311657254 2117 6 0.3682833436793089 2117 7 0.5810516312281848 2117 8 0.31812648817483336 2117 9 -0.5536922825562414 2117 10-11 -0.493327970003655 2117 12-13 -1.5002873314566614 2117 14-15 -0.5074962699674472 2117 16-17 -1.1668802300659493 2117 18-19 -1.5927562493595389 2117 20-21 -0.5019749057520713 2117 22-23 0.3486688452413631 2117 24-25 -1.7243686393015878 2117 26-27 -2.741251343487775 2117 28-29 -1.7869148478918078 2117 30-31 -0.5581594271973884 2117 32-33 -2.6223712408818542 2117 34-35 0.01744326828073639 2117 36-37 -2.604797639759674 2117 38-39 0.28233481704884866 2117 40-41 -1.816257198709934 2117 42-43 0.04099194002788664 2117 44-45 -1.0709135221039787 2117 46-47 0.002216642592991036 2117 48-49 -0.7294070764339793 2117 50-51 -0.674920282793849 2117 52-53 0.35965316581798845 2117 54-55 -0.4182774627798693 2117 56-57 -0.1076915716846969 2117 58-59 -2.0666655009216086 2117 60-61 -0.8868516185177242 2117 62-63 -0.29167197891995045 2117 64-65 -1.1249163132946691 2117 66-67 -0.6666842292244084 2117 68-69 -0.4705028725901741 2117 70-71 -0.6448043449987679 2117 72-73 1.185699786929625 2117 74-75 1.0185571879705932 2117 76 1.2218861300154913 2118 1 0.1255541595862013 2118 2 -0.12483170347150008 2118 3 -0.582199066318644 2118 4 0.4828856078113901 2118 5 0.38263016658955706 2118 6 -0.24100098900927946 2118 7 0.34507484205990124 2118 8 0.07900656554427599 2118 9 -2.175065590099763 2118 10-11 -1.2578347398295762 2118 12-13 -1.5749971960601457 2118 14-15 0.19753274357221073 2118 16-17 -2.738930520201343 2118 18-19 -0.6553771391080829 2118 20-21 -1.061089992792688 2118 22-23 0.5353226170015191 2118 24-25 0.8727058287835234 2118 26-27 -2.5473441868146622 2118 28-29 -1.1338684262283678 2118 30-31 -0.415993082903384 2118 32-33 0.11288020979512936 2118 34-35 -1.0455402907134612 2118 36-37 -2.592466885407646 2118 38-39 -0.10124835509815 2118 40-41 -0.4169583592515167 2118 42-43 0.24795518954432083 2118 44-45 -2.791537313206497 2118 46-47 -0.7419806494766377 2118 48-49 0.1408782233725958 2118 50-51 -0.6370817914979128 2118 52-53 -1.6702066020736908 2118 54-55 -0.6002165343466004 2118 56-57 -0.5256074324197044 2118 58-59 -1.2789478993742165 2118 60-61 -1.391445428962598 2118 62-63 -1.3246748802739177 2118 64-65 -0.025424050238576967 2118 66-67 -0.9637103414100991 2118 68-69 -1.7933993909654191 2118 70-71 -0.5234310374552464 2118 72-73 1.1523342163300114 2118 74-75 -0.8123180538089017 2118 76 -0.37506744832106165 2119 1 0.7787995137472521 2119 2 -0.5790466240209184 2119 3 -0.4985739653221444 2119 4 -0.5646500776181824 2119 5 -0.27654031550039804 2119 6 -0.35438639164865293 2119 7 -1.3522049855727403 2119 8 0.20060635008507433 2119 9 -0.7357981513709717 2119 10-11 -0.3661703175295443 2119 12-13 0.17735938891158298 2119 14-15 0.26580637675291996 2119 16-17 -0.3420816109635396 2119 18-19 -1.117065800567815 2119 20-21 -1.9059593706542515 2119 22-23 -0.05725750688751674 2119 24-25 0.4378149050022131 2119 26-27 -0.17911095223345797 2119 28-29 -1.1645040308607335 2119 30-31 0.6374677304550858 2119 32-33 -0.1751618047483703 2119 34-35 0.21530000015645356 2119 36-37 0.02192848221961441 2119 38-39 -2.0868395212710595 2119 40-41 -1.6301956336926722 2119 42-43 0.45008284911879315 2119 44-45 0.18994666201569288 2119 46-47 -0.16953249434602213 2119 48-49 -0.7004226115317991 2119 50-51 0.39995025805770723 2119 52-53 0.3813016123082136 2119 54-55 -0.09038620846456524 2119 56-57 -1.5041961746766361 2119 58-59 -0.3776551441897311 2119 60-61 0.6308411317699694 2119 62-63 -0.3087174334654037 2119 64-65 -0.1505923777365581 2119 66-67 0.22737503429687678 2119 68-69 0.8336542045099407 2119 70-71 -0.10510353947517359 2119 72-73 0.8370231471137402 2119 74-75 -0.7231718108786609 2119 76 0.19239821286934955 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 0.0 21 2.0 22 1.0 23 6.0 24 12.0 25 30.0 26 71.0 27 150.0 28 280.0 29 373.0 30 721.0 31 1041.0 32 1638.0 33 2461.0 34 3782.0 35 5513.0 36 9410.0 37 13649.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.348074908663556 24.75920390383485 10.860734268414195 28.031986919087405 2 17.263227817378198 19.557497253519326 39.73327201655553 23.446002912546945 3 16.665389233795764 25.875680233003756 31.749316573414067 25.709613959786417 4 12.373214787562913 20.589663013208657 40.37454331774865 26.662578881479774 5 12.018088449451982 38.596356761452185 36.830944533864745 12.554610255231088 6 26.228251705372884 40.85996780869166 18.456350118801257 14.455430367134205 7 25.377481413351727 31.820852814184615 23.76025139878899 19.041414373674666 8 22.125137324033624 36.48603765872103 21.03165478654097 20.357170230704376 9 22.268209805574717 15.037939756265809 22.41128228711581 40.28256815104366 10-11 23.919930507651824 25.82330548529675 28.494417618354156 21.762346388697274 12-13 23.09343144017782 21.985897141105234 27.95278608109144 26.967885337625507 14-15 20.285633989933828 24.948263968728444 24.241843591119284 30.52425845021844 16-17 22.73575023632508 28.062645307989065 25.63169055466135 23.5699139010245 18-19 22.892874479446103 27.05091847423418 25.92294524922715 24.133261797092562 20-21 21.39955545336093 27.46097442579393 25.235686364681538 25.903783756163612 22-23 22.057433381875782 28.269589433075293 27.13395161084285 22.539025574206075 24-25 22.425334048695742 29.149740681127206 25.749214378784398 22.675710891392658 26-27 21.646099997445134 29.120359725096446 26.25124549704913 22.98229478040929 28-29 20.818323497100227 29.437163077080296 27.27957895812575 22.464934467693723 30-31 21.740630029891932 28.697529444827673 26.612758999514575 22.94908152576582 32-33 20.998441531897498 29.369459134922458 27.467361590148435 22.164737743031605 34-35 23.212232697171764 27.28341125673846 28.430545974809025 21.073810071280754 36-37 23.272272042104188 26.955111008916482 27.036866712654252 22.73575023632508 38-39 21.758514090084567 26.79032216857004 27.90552106486804 23.545642676477353 40-41 21.012493293477426 26.25252292992003 29.053933215809508 23.68105056079303 42-43 22.586290590429474 26.054520834930123 29.134411486676377 22.22477708796403 44-45 20.4172095756368 26.80692879589178 28.646432129991567 24.129429498479855 46-47 22.16729260877341 25.488618073120257 28.617051173960807 23.727038144145528 48-49 21.45320763393884 25.452849952734987 28.963235481975424 24.13070693135076 50-51 20.095296492169336 26.027694744641167 29.522751079430776 24.354257683758718 52-53 20.100406223652946 25.842466978360285 31.17702664724969 22.88010015073708 54-55 19.2062032140211 26.405814874428344 29.788457116578527 24.599524794972023 56-57 21.03293221941187 26.022585013157556 28.542960067448455 24.401522699982117 58-59 19.27646202192075 27.52612350220996 29.48953782478731 23.707876651081985 60-61 20.764671316522318 28.670703354538716 27.067525101555912 23.49710022738305 62-63 18.484453641961114 30.350527579775683 26.958943307529186 24.206075470734014 64-65 19.04907897090008 31.307324800081755 25.67767813801385 23.96591809100432 66-67 19.13722183899236 30.726092843821057 25.523108760634628 24.61357655655195 68-69 19.14233157047597 30.036279093533636 25.438798191155055 25.382591144835338 70-71 19.27646202192075 30.193403336654654 25.629135688919547 24.900998952505045 72-73 19.603484836871825 28.6132188753481 25.138601466492936 26.64469482128714 74-75 19.96116604072456 29.544467438236122 24.881837459441506 25.61252906159781 76 19.5932653739046 30.65838890166322 24.3938581027567 25.35448762167548 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 1.5 16 1.5 17 0.0 18 3.0 19 6.0 20 5.5 21 5.0 22 8.0 23 20.5 24 37.5 25 45.0 26 77.0 27 130.5 28 162.5 29 173.0 30 235.5 31 329.0 32 484.5 33 609.0 34 675.0 35 871.0 36 1070.0 37 1139.0 38 1371.5 39 1894.5 40 2481.0 41 2920.0 42 3063.0 43 3090.0 44 3103.0 45 3135.5 46 3182.0 47 3144.0 48 2858.0 49 2429.0 50 2248.0 51 2047.5 52 1643.0 53 1304.5 54 1170.0 55 1090.0 56 808.5 57 561.5 58 516.0 59 411.0 60 260.0 61 182.0 62 150.0 63 109.5 64 61.0 65 45.5 66 31.5 67 25.0 68 20.0 69 9.0 70 4.0 71 5.0 72 4.5 73 2.0 74 0.5 75 1.0 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 39141.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.031016070105515 #Duplication Level Percentage of deduplicated Percentage of total 1 62.1413109840706 28.604276845251782 2 15.59083088194483 14.353235737461997 3 8.253316312371648 11.397256074193301 4 4.712216240217573 8.67632405917069 5 2.875062441027918 6.617102271275645 6 1.8094022312260643 4.997317390971104 7 1.1822167952489315 3.8093048210316547 8 0.7936948437586723 2.9227664086252267 9 0.6660376311261585 2.7592550011496892 >10 1.95926069822945 13.063028537850336 >50 0.0 0.0 >100 0.011100627185435977 1.4230602181855343 >500 0.005550313592717989 1.3770726348330395 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT 539 1.3770726348330395 TruSeq Adapter, Index 5 (95% over 23bp) ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT 430 1.0985922689762653 TruSeq Adapter, Index 5 (96% over 26bp) ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 127 0.3244679492092691 No Hit CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC 48 0.12263355560665286 RNA PCR Primer, Index 5 (95% over 21bp) TCTGTACCCACGCCTTCTTCAAAGCCATACTATTTATGTGCTCCGGATCC 42 0.10730436115582126 No Hit CTTTACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC 40 0.10219462967221073 RNA PCR Primer, Index 5 (95% over 24bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.010219462967221073 0.0 2 0.0 0.0 0.0 0.010219462967221073 0.0 3 0.0 0.0 0.0 0.010219462967221073 0.0 4 0.0 0.0 0.0 0.010219462967221073 0.0 5 0.0 0.0 0.0 0.010219462967221073 0.0 6 0.0 0.0 0.0 0.010219462967221073 0.0 7 0.0 0.0 0.0 0.01277432870902634 0.0 8 0.0 0.0 0.0 0.015329194450831609 0.0 9 0.0 0.0 0.0 0.022993791676247412 0.0 10 0.0 0.0 0.0 0.028103523159857948 0.0 11 0.0 0.0 0.0 0.04343271761068956 0.0 12 0.0 0.0 0.0 0.05876191206152117 0.0 13 0.0 0.0 0.0 0.05876191206152117 0.0 14 0.0 0.0 0.0 0.07409110651235278 0.0 15 0.0 0.0 0.0 0.07920083799596331 0.0 16 0.0 0.0 0.0 0.08431056947957385 0.0 17 0.0 0.0 0.0 0.09197516670498965 0.0 18 0.0 0.0 0.0 0.09197516670498965 0.0 19 0.0 0.0 0.0 0.09453003244679492 0.0 20 0.0 0.0 0.0 0.09453003244679492 0.0 21 0.0 0.0 0.0 0.09453003244679492 0.0 22 0.0 0.0 0.0 0.0970848981886002 0.0 23 0.0 0.0 0.0 0.0970848981886002 0.0 24 0.0 0.0 0.0 0.10219462967221073 0.0 25 0.0 0.0 0.0 0.104749495414016 0.0 26 0.0 0.0 0.0 0.11241409263943179 0.0 27 0.0 0.0 0.0 0.12774328709026342 0.0 28 0.0 0.0 0.0 0.15073707876651082 0.0 29 0.0 0.0 0.0 0.16351140747553716 0.0 30 0.0 0.0 0.0 0.18139546766817405 0.0 31 0.0 0.0 0.0 0.21205385656983725 0.0 32 0.0 0.0 0.0 0.25293170843872154 0.0 33 0.0 0.0 0.0 0.29125469456580055 0.0 34 0.0 0.0 0.0 0.35768120385273755 0.0 35 0.0 0.0 0.0 0.4241077131396745 0.0 36 0.0 0.0 0.0 0.5390766715209115 0.0 37 0.0 0.0 0.0 0.7230270049308909 0.0 38 0.0 0.0 0.0 1.057714417107381 0.0 39 0.0 0.0 0.0 1.4383894126363659 0.0 40 0.0 0.0 0.0 1.9238139035793669 0.0 41 0.0 0.0 0.0 2.4041286630387573 0.0 42 0.0 0.0 0.0 2.9355407373342532 0.0 43 0.0 0.0 0.0 3.505275797756828 0.0 44 0.0 0.0 0.0 4.105669247081066 0.0 45 0.0 0.0 0.0 4.675404307503641 0.0 46 0.0 0.0 0.0 5.278352622569684 0.0 47 0.0 0.0 0.0 5.842977951508648 0.0 48 0.0 0.0 0.0 6.422932474898444 0.0 49 0.0 0.0 0.0 7.061648910349761 0.0 50 0.0 0.0 0.0 7.600725581870672 0.0 51 0.0 0.0 0.0 8.157686313584222 0.0 52 0.0 0.0 0.0 8.750415165683043 0.0 53 0.0 0.0 0.0 9.269052911269513 0.0 54 0.0 0.0 0.0 9.90265961523722 0.0 55 0.0 0.0 0.0 10.503053064561458 0.0 56 0.0 0.0 0.0 11.0089164814389 0.0 57 0.0 0.0 0.0 11.545438287218007 0.0 58 0.0 0.0 0.0 11.946552208681434 0.0 59 0.0 0.0 0.0 12.393653713497356 0.0 60 0.0 0.0 0.0 12.894407398891188 0.0 61 0.0 0.0 0.0 13.359392963899747 0.0 62 0.0 0.0 0.0 13.770726348330395 0.0 63 0.0 0.0 0.0 14.304693288367696 0.0 64 0.0 0.0 0.0 14.790117779310696 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTGCGG 15 0.002208822 70.0 33 GAGGCAC 15 0.002208822 70.0 7 TTGTCGT 15 0.002208822 70.0 3 ATTAGAG 15 0.002208822 70.0 3 ATAGGGA 15 0.002208822 70.0 41 TGTCAGG 15 0.002208822 70.0 29 TGTTATA 15 0.002208822 70.0 24 TTATAAT 15 0.002208822 70.0 26 AGTACTG 15 0.002208822 70.0 30 GACACAT 15 0.002208822 70.0 26 AGTACAA 15 0.002208822 70.0 49 GCAGATG 15 0.002208822 70.0 12 TACTGCG 15 0.002208822 70.0 32 AGATGGA 15 0.002208822 70.0 14 CTTGTTA 15 0.002208822 70.0 22 GTGACAC 15 0.002208822 70.0 24 GTAGGCA 15 0.002208822 70.0 8 TCGTAGG 15 0.002208822 70.0 6 GTTATAA 15 0.002208822 70.0 25 CAGTGAC 15 0.002208822 70.0 22 >>END_MODULE