FastQCFastQC Report
Wed 25 May 2016
SRR1780570_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780570_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156809
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCTAA150.00222215669.993316
AGACCGC150.00222215669.9933114
TGTGTCC150.00222215669.993318
TGTAACG150.00222215669.9933133
GGACCAT207.911064E-569.993327
CGGCGGC308.351262E-658.3277559
GTTGGCG308.351262E-658.32775557
ATGGACC200.006943528552.49497625
GACCGCA200.006943528552.49497615
GACCATG200.006943528552.49497628
AGGCCCG200.006943528552.49497621
GAGCACC200.006943528552.49497667
GCAGCTA200.006943528552.49497624
CGCAACC200.006943528552.49497618
AGTTGGC352.0803356E-549.99521656
GCGGCTT352.0803356E-549.99521611
ATATTAG352.0803356E-549.99521651
CGGATGC305.866563E-446.6622059
GGCGGCG305.866563E-446.6622057
CGTCAAA305.866563E-446.66220565
Produced by FastQC (version 0.11.5)