##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780570_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 156809 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.896970199414575 34.0 31.0 34.0 12.0 34.0 2 31.36839084491324 34.0 32.0 34.0 23.0 34.0 3 31.598058784891172 34.0 34.0 34.0 23.0 34.0 4 31.869529172432706 34.0 34.0 34.0 23.0 34.0 5 31.791638235050286 34.0 34.0 34.0 23.0 34.0 6 34.8084038543706 38.0 36.0 38.0 26.0 38.0 7 33.97005911650479 38.0 34.0 38.0 23.0 38.0 8 33.07191551505335 37.0 34.0 38.0 21.0 38.0 9 33.61986875753305 37.0 34.0 38.0 21.0 38.0 10-11 34.05552614964702 37.0 34.0 38.0 23.0 38.0 12-13 34.452627081353754 38.0 35.0 38.0 25.0 38.0 14-15 33.98421646716706 38.0 34.0 38.0 22.5 38.0 16-17 33.86679654866749 37.0 34.0 38.0 22.0 38.0 18-19 33.854048555886465 37.0 34.0 38.0 22.0 38.0 20-21 33.917683296239375 37.0 34.0 38.0 22.5 38.0 22-23 33.89855492988285 37.0 34.0 38.0 22.0 38.0 24-25 34.319783940972776 38.0 35.0 38.0 23.5 38.0 26-27 34.39639625276611 38.0 35.5 38.0 24.0 38.0 28-29 34.541926802670766 38.0 36.0 38.0 24.5 38.0 30-31 34.38147045131338 38.0 35.0 38.0 24.0 38.0 32-33 34.11501890835348 38.0 34.0 38.0 23.0 38.0 34-35 34.01472173153327 37.0 34.0 38.0 23.0 38.0 36-37 33.99919009750715 37.0 34.0 38.0 23.0 38.0 38-39 34.526187910132705 38.0 35.5 38.0 25.0 38.0 40-41 33.99251637342245 37.5 34.0 38.0 23.0 38.0 42-43 34.30302469883744 38.0 34.5 38.0 24.0 38.0 44-45 33.39978891517706 37.0 34.0 38.0 21.5 38.0 46-47 34.100096295493245 37.0 34.0 38.0 24.0 38.0 48-49 33.83631041585623 37.0 34.0 38.0 22.5 38.0 50-51 33.79638286067764 37.0 34.0 38.0 22.0 38.0 52-53 33.78153039685222 37.0 34.0 38.0 22.5 38.0 54-55 33.47623860875332 37.0 34.0 38.0 21.0 38.0 56-57 33.59723931662086 37.0 34.0 38.0 22.0 38.0 58-59 33.47528521959836 37.0 33.5 38.0 22.0 38.0 60-61 33.50215867711675 37.0 33.5 38.0 21.5 38.0 62-63 33.57634128143155 37.0 34.0 38.0 22.0 38.0 64-65 33.58120707357358 37.0 34.0 38.0 22.0 38.0 66-67 33.524580859516995 37.0 33.5 38.0 22.0 38.0 68-69 33.10162044270418 37.0 32.5 38.0 20.5 38.0 70-71 32.9588001964173 37.0 32.0 38.0 20.0 38.0 72-73 33.084497701024816 37.0 32.0 38.0 20.0 38.0 74-75 32.739329375227186 37.0 31.5 38.0 19.5 38.0 76 31.984669247300857 36.0 30.0 38.0 19.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.085439063350357 1101 2 -0.47399147108749773 1101 3 -0.0101645922717708 1101 4 -2.0676852168867548 1101 5 -0.44716223808806177 1101 6 -1.9995559998499601 1101 7 -0.8407962660833945 1101 8 -0.8352366913124172 1101 9 -0.8788676432180722 1101 10-11 -0.6954749079308442 1101 12-13 -0.8932563853380273 1101 14-15 0.16574055368040064 1101 16-17 -1.6502374983223902 1101 18-19 -0.1704688343177594 1101 20-21 -0.6995201538022329 1101 22-23 0.06996687610254781 1101 24-25 -1.0310202541328337 1101 26-27 -0.6277857040764019 1101 28-29 -0.09438398963228423 1101 30-31 -1.245321805082881 1101 32-33 0.5592653734622601 1101 34-35 -0.4984873136703243 1101 36-37 0.26951310662922623 1101 38-39 -0.3181560379253625 1101 40-41 0.5209035485600424 1101 42-43 0.19376441993432536 1101 44-45 0.4251549485488013 1101 46-47 0.38439015557765543 1101 48-49 -0.8651982165996728 1101 50-51 -0.4784253946965862 1101 52-53 0.3177050793505387 1101 54-55 -0.43219019655354174 1101 56-57 -0.21678744636181335 1101 58-59 0.17136332463274329 1101 60-61 0.059684270068466105 1101 62-63 -1.4741620480467859 1101 64-65 0.22695609300879482 1101 66-67 -0.9531280516614942 1101 68-69 0.40082379550246117 1101 70-71 0.6767119453844543 1101 72-73 -0.32252918216815374 1101 74-75 -0.7496850097647467 1101 76 -1.4577267170684571 1102 1 0.8052703564288279 1102 2 0.2777116061969771 1102 3 0.1189169205306726 1102 4 0.3962565261247555 1102 5 0.8447686991917109 1102 6 0.9744280264596483 1102 7 1.7561616855257185 1102 8 2.038207048410392 1102 9 1.4350858451540205 1102 10-11 0.5506784864783754 1102 12-13 -3.6124835406116915 1102 14-15 -0.6665063337990418 1102 16-17 -1.946191221601694 1102 18-19 -0.2564742371836317 1102 20-21 0.7592829939544075 1102 22-23 -0.2544987099909477 1102 24-25 0.7616741315848046 1102 26-27 -4.7805928452556365 1102 28-29 0.3464193930315602 1102 30-31 1.0453758693357216 1102 32-33 -5.913367936380354 1102 34-35 -0.08631324742648872 1102 36-37 1.3021064822458541 1102 38-39 1.250290050869559 1102 40-41 0.9511948335024982 1102 42-43 1.0038713027156305 1102 44-45 -5.474480945273136 1102 46-47 0.3108935617322288 1102 48-49 0.862074510673061 1102 50-51 -5.8191583051969324 1102 52-53 -0.912562714870738 1102 54-55 1.5473141492298836 1102 56-57 -0.8764068966789438 1102 58-59 1.2176400453280678 1102 60-61 0.9141592524504247 1102 62-63 -4.6205092409056086 1102 64-65 0.06393048812272895 1102 66-67 0.7171209499828564 1102 68-69 -7.17458141943764 1102 70-71 -0.09563360312388625 1102 72-73 0.8431391288490033 1102 74-75 -5.994575777911329 1102 76 -0.9158545065915931 1103 1 0.5766274248710985 1103 2 0.15027128368938847 1103 3 0.043612304867309604 1103 4 0.5293588498100412 1103 5 0.5410476320177295 1103 6 -0.9207909797459095 1103 7 1.1099574911152459 1103 8 1.5663924902729605 1103 9 0.8481401550693164 1103 10-11 1.1266795282865871 1103 12-13 -0.7561171864874936 1103 14-15 -0.2042435716483908 1103 16-17 -0.5193612628210644 1103 18-19 -0.09135329683847715 1103 20-21 -0.24362161264975413 1103 22-23 -0.02340979392445064 1103 24-25 0.76444851216187 1103 26-27 -0.24479767381023976 1103 28-29 0.6615777025844949 1103 30-31 0.19348249614190394 1103 32-33 -4.986475695586769 1103 34-35 -0.8752848015325725 1103 36-37 0.1826948774082382 1103 38-39 0.7140249333264208 1103 40-41 0.6388222285289942 1103 42-43 0.5928270032184102 1103 44-45 -4.714504499198036 1103 46-47 0.14580659339414126 1103 48-49 0.1421510609289811 1103 50-51 -0.0780476470459206 1103 52-53 -0.3072268449608728 1103 54-55 0.4411629511504316 1103 56-57 0.9748296843782711 1103 58-59 1.0178280237671657 1103 60-61 1.2275849896429776 1103 62-63 0.5606521487759579 1103 64-65 0.9135703848478229 1103 66-67 0.18917331216974986 1103 68-69 -3.4384306743182833 1103 70-71 0.5678227088750276 1103 72-73 1.300957220075162 1103 74-75 -1.1831996851451052 1103 76 0.7822589146159942 1104 1 -1.6151548256242592 1104 2 -0.12696304731488794 1104 3 -0.1900095535120805 1104 4 -0.32995911869554106 1104 5 -0.6755860106978808 1104 6 -0.3830185321497055 1104 7 1.198997015570356 1104 8 1.0900863061036006 1104 9 -0.3762836638899074 1104 10-11 -0.45581082524350336 1104 12-13 -0.4941607781028807 1104 14-15 -0.25079691401985826 1104 16-17 -0.49235636137148475 1104 18-19 0.4761719925556207 1104 20-21 0.7387227402546159 1104 22-23 -0.2417902298406034 1104 24-25 0.24804951330902014 1104 26-27 0.849862874734967 1104 28-29 -0.37771732296561566 1104 30-31 -0.9563037172275841 1104 32-33 -1.0661868229201659 1104 34-35 -0.21579997516903404 1104 36-37 -0.044440381742866464 1104 38-39 -0.5766831697083106 1104 40-41 0.7101800343481557 1104 42-43 0.6041003372469831 1104 44-45 -3.352235232329754 1104 46-47 -0.019356614448177822 1104 48-49 0.6589619901186765 1104 50-51 0.8399208585333895 1104 52-53 -0.3238969878329172 1104 54-55 -0.6883917469411287 1104 56-57 0.38592573193275115 1104 58-59 0.844619138586232 1104 60-61 0.020149386347533493 1104 62-63 0.45917128528654416 1104 64-65 -0.007281633089398554 1104 66-67 0.3238745323178307 1104 68-69 -3.2292795636706657 1104 70-71 -0.5617893466052166 1104 72-73 0.6215276653770729 1104 74-75 -0.9796591699714661 1104 76 0.2955032570917524 1105 1 -0.533785032626561 1105 2 0.1328120612974324 1105 3 -0.036316734702982245 1105 4 0.29715562702385867 1105 5 0.2898624942855079 1105 6 0.3231404251720491 1105 7 1.8392730186370514 1105 8 1.2219288821322252 1105 9 1.1949149049830794 1105 10-11 1.212563823363709 1105 12-13 -0.8144648925930511 1105 14-15 0.19193244963974365 1105 16-17 1.528164977754507 1105 18-19 0.8970638913544917 1105 20-21 0.4950222546936658 1105 22-23 0.7828583773661464 1105 24-25 1.037520507431175 1105 26-27 0.8519967873339951 1105 28-29 -0.6378629912508202 1105 30-31 0.39824522220508385 1105 32-33 1.1470383240259068 1105 34-35 1.063423127309882 1105 36-37 1.4864055165448846 1105 38-39 1.1432887188682272 1105 40-41 0.940655372963036 1105 42-43 1.218564942409266 1105 44-45 1.7942891734969777 1105 46-47 1.3843617602004272 1105 48-49 1.0068408554393997 1105 50-51 0.558142172103544 1105 52-53 -0.182087634395657 1105 54-55 -0.1181259662102363 1105 56-57 0.4453047250326776 1105 58-59 0.5864157141037367 1105 60-61 0.771235509526683 1105 62-63 0.9941316737353034 1105 64-65 0.7745864987787385 1105 66-67 0.8642016470635525 1105 68-69 1.8553997105434874 1105 70-71 1.8637153812250986 1105 72-73 1.0624808981907705 1105 74-75 -0.2734746568102082 1105 76 -1.1223091630462498 1106 1 -0.823517522359051 1106 2 -0.20208637360100568 1106 3 -1.5376487360349849 1106 4 -0.8007131708449435 1106 5 -0.21583736141434784 1106 6 -0.5962790442474244 1106 7 0.5541414835055178 1106 8 0.3210353312386687 1106 9 0.8467020067701796 1106 10-11 0.10749666829654814 1106 12-13 -0.19202899515714478 1106 14-15 -0.4430214853141976 1106 16-17 -0.159575059985535 1106 18-19 -0.6137974695068635 1106 20-21 -0.8114240767526653 1106 22-23 -0.6067714372636672 1106 24-25 -0.1385783936677214 1106 26-27 -0.5318554715182628 1106 28-29 -1.5626715160593463 1106 30-31 -1.0985635246036694 1106 32-33 0.2810765080640891 1106 34-35 0.37328271216947684 1106 36-37 0.06347011860949436 1106 38-39 -0.045164494584987835 1106 40-41 -0.422542540234879 1106 42-43 -0.11607819223387139 1106 44-45 0.9548119840197913 1106 46-47 0.2667016425403048 1106 48-49 0.06535733895588436 1106 50-51 0.02680129076266269 1106 52-53 -0.406249583557603 1106 54-55 -0.24056463864891242 1106 56-57 0.2902597699877205 1106 58-59 -0.36695591426789065 1106 60-61 0.26895723224840395 1106 62-63 0.10423823384186193 1106 64-65 0.2659506901429225 1106 66-67 0.25121185907376287 1106 68-69 0.6991559542997337 1106 70-71 -0.005987138477422604 1106 72-73 0.1550078157176813 1106 74-75 -1.8140202223557722 1106 76 -0.9587837995208872 1107 1 -0.7374917012338891 1107 2 -0.12091037520646353 1107 3 -0.11129914743374059 1107 4 -0.289274278611348 1107 5 -0.5462775246359683 1107 6 0.02130407168006343 1107 7 -0.2876706778430673 1107 8 0.3402172163145991 1107 9 -1.1966286507988855 1107 10-11 -0.6859176069058037 1107 12-13 -0.06537336601807908 1107 14-15 -1.283144570470391 1107 16-17 -0.029817216651530032 1107 18-19 0.3572875529026618 1107 20-21 0.0676564637517103 1107 22-23 -1.4703158215034193 1107 24-25 -1.1267130515043675 1107 26-27 -0.0830879954328978 1107 28-29 -1.4242007153368874 1107 30-31 -0.5929611431734756 1107 32-33 0.5610450678339802 1107 34-35 -0.021400878706174353 1107 36-37 -1.0557602646937383 1107 38-39 0.2906021118769857 1107 40-41 0.009643559169759897 1107 42-43 -0.03312950001365067 1107 44-45 -0.5341003178064128 1107 46-47 -0.32554533050136314 1107 48-49 0.08439707405522512 1107 50-51 0.7515487655101367 1107 52-53 0.7197705515684873 1107 54-55 0.029871510926312794 1107 56-57 0.5524512209208936 1107 58-59 0.46434551057658524 1107 60-61 -0.5928646287456374 1107 62-63 -0.7883465544382844 1107 64-65 -0.16876066178696902 1107 66-67 0.6802121266501899 1107 68-69 0.9818170018614438 1107 70-71 -0.7421066113518648 1107 72-73 -0.6920638947646154 1107 74-75 -0.7735910966617467 1107 76 -1.0038704273889714 1108 1 -1.052732751574279 1108 2 -0.6997553712700011 1108 3 -0.3033830017692516 1108 4 -0.7832306533624234 1108 5 -0.5190619146389004 1108 6 0.1727574747890941 1108 7 0.6840948634588955 1108 8 1.0018822620856014 1108 9 -0.5272296171614457 1108 10-11 0.09914390994379119 1108 12-13 0.37178219365404175 1108 14-15 0.3580663157736055 1108 16-17 -1.0742992247096979 1108 18-19 -0.06387516958456274 1108 20-21 0.2065440662154785 1108 22-23 -0.5177077231999547 1108 24-25 -0.7635783936677214 1108 26-27 -0.9130714777342703 1108 28-29 0.39537044198261384 1108 30-31 0.4315452555051138 1108 32-33 0.11460409159167284 1108 34-35 -0.0252215613348028 1108 36-37 -0.2159587858194172 1108 38-39 0.45920613478564576 1108 40-41 0.505390637698298 1108 42-43 0.37537479921912364 1108 44-45 0.013669792877600173 1108 46-47 0.3280847039233734 1108 48-49 0.16238531098385778 1108 50-51 0.1769566909180611 1108 52-53 0.0930705407625183 1108 54-55 0.247974599890334 1108 56-57 0.4860639657919208 1108 58-59 -1.0519792242912018 1108 60-61 0.8453946836858606 1108 62-63 0.31461119421482664 1108 64-65 0.44621487040711116 1108 66-67 -0.27918829632638875 1108 68-69 0.9031186582624429 1108 70-71 1.6651472826570028 1108 72-73 0.6603496960595692 1108 74-75 1.095556312220765 1108 76 0.41495357421649004 1109 1 -0.3717020705435985 1109 2 -0.628785800300431 1109 3 0.32332587493962706 1109 4 -0.088182458314229 1109 5 0.2881893926124093 1109 6 0.20258800461962778 1109 7 -1.1917635123994756 1109 8 0.17736893757227534 1109 9 0.6880888481570224 1109 10-11 0.616130301930184 1109 12-13 0.05909713096897917 1109 14-15 -0.09951889181160567 1109 16-17 -0.8749040253144997 1109 18-19 0.18906220835281573 1109 20-21 0.7539594886308976 1109 22-23 0.89595856546633 1109 24-25 0.7668270117376821 1109 26-27 0.09577215610936918 1109 28-29 0.3257396223517901 1109 30-31 0.3456761696360289 1109 32-33 0.8407270677146528 1109 34-35 0.05308751697427283 1109 36-37 0.17096397610334435 1109 38-39 0.8477511483306586 1109 40-41 0.05807444038210008 1109 42-43 0.1969507457950712 1109 44-45 0.0776621068699157 1109 46-47 -0.4162767904381255 1109 48-49 -0.3366696880711473 1109 50-51 1.0060532700146396 1109 52-53 0.6123485600405374 1109 54-55 -0.03144642953069621 1109 56-57 0.40860538833334203 1109 58-59 0.05513663282465586 1109 60-61 0.6227997110908845 1109 62-63 -0.2081082035045725 1109 64-65 0.3796553038475423 1109 66-67 0.4533685612304694 1109 68-69 1.4414778216216035 1109 70-71 0.20852282603254224 1109 72-73 -1.0162316055217318 1109 74-75 1.4781241947886414 1109 76 0.2450109042738191 1110 1 0.3370838181488338 1110 2 0.7528130883078035 1110 3 0.3864520852620963 1110 4 0.38287504779000514 1110 5 0.7853898641119414 1110 6 0.710102528489287 1110 7 1.3093099910104726 1110 8 2.4109296010394843 1110 9 -1.0106566262893892 1110 10-11 -0.6260183888166324 1110 12-13 0.23476228210141414 1110 14-15 0.20575600925769066 1110 16-17 0.4239824780112613 1110 18-19 0.9846755787325421 1110 20-21 0.2186075479051297 1110 22-23 0.6420013372100399 1110 24-25 0.6336178680145181 1110 26-27 -0.5662531869907426 1110 28-29 0.7445006731221895 1110 30-31 0.568870158016928 1110 32-33 1.3791837486946932 1110 34-35 0.8648455366762207 1110 36-37 0.6058802007392003 1110 38-39 0.9088145481791017 1110 40-41 0.8206723186341875 1110 42-43 1.2203468054060878 1110 44-45 1.7586211032494745 1110 46-47 0.7028505144368467 1110 48-49 1.4368526660586909 1110 50-51 0.9174366159774223 1110 52-53 0.6428276372485797 1110 54-55 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0.2260708041567696 2116 44-45 1.5446889023028803 2116 46-47 0.2676494236937472 2116 48-49 0.43714397134663585 2116 50-51 0.35180583491887774 2116 52-53 0.8664419850851175 2116 54-55 0.13486705619152417 2116 56-57 -0.07851798182344538 2116 58-59 -0.8681536240131607 2116 60-61 -1.6668955111236166 2116 62-63 0.17339379978406555 2116 64-65 0.5931039710237158 2116 66-67 -0.37605372738205034 2116 68-69 -0.02040469071078377 2116 70-71 -2.8494799067773897 2116 72-73 0.44374025579973875 2116 74-75 -1.4746074571717998 2116 76 -0.9332586248954549 2117 1 -0.08361853246005779 2117 2 -0.027843949358580034 2117 3 0.5456845972983508 2117 4 -0.4800061001378708 2117 5 -0.7410898866668738 2117 6 -1.4321376301060127 2117 7 -2.9509090215449874 2117 8 -2.2193687091653693 2117 9 -0.03966162959345354 2117 10-11 -0.37103868523880124 2117 12-13 0.46453666140850913 2117 14-15 -0.8394861317788482 2117 16-17 0.0853744349639598 2117 18-19 0.1551419244325274 2117 20-21 -0.2533432686718555 2117 22-23 0.20004674455451266 2117 24-25 0.13793675784742732 2117 26-27 0.6676394779766852 2117 28-29 0.4499547465669167 2117 30-31 -0.16422009026023687 2117 32-33 0.6005209525085391 2117 34-35 -0.020656681769928298 2117 36-37 -1.2496611945218277 2117 38-39 0.30332035389987055 2117 40-41 -1.460421328113668 2117 42-43 -0.9039569801126603 2117 44-45 0.4611251153329192 2117 46-47 -0.01865189281323154 2117 48-49 -0.7818648832663371 2117 50-51 0.7010437150050848 2117 52-53 0.5078918305838087 2117 54-55 -1.0764232245074936 2117 56-57 -0.6630230582951029 2117 58-59 -0.26342056073253417 2117 60-61 0.6351188484100234 2117 62-63 -0.514448634845003 2117 64-65 -0.49793819874596323 2117 66-67 -1.2285861207242093 2117 68-69 -0.17836929822551895 2117 70-71 1.0445179120276293 2117 72-73 1.3961694318793008 2117 74-75 0.08875755542200636 2117 76 -0.6027231934602817 2118 1 -0.18736880110394694 2118 2 0.11158222730163914 2118 3 0.3171761081515605 2118 4 0.29137795316107784 2118 5 -0.2215863405675833 2118 6 0.2292636164016173 2118 7 0.2598689711479025 2118 8 -4.261868171877598 2118 9 -1.5353633274228145 2118 10-11 -0.766616806880748 2118 12-13 0.38628069751424476 2118 14-15 -1.0417749776847103 2118 16-17 0.23914619511869972 2118 18-19 -0.5575434398485797 2118 20-21 -2.4873589574747825 2118 22-23 -0.6084101649662301 2118 24-25 -0.5673608995778849 2118 26-27 0.2302603677252435 2118 28-29 -0.6954890531321993 2118 30-31 -0.7678629013498508 2118 32-33 -0.035654633135131064 2118 34-35 0.6537200644153316 2118 36-37 -0.5091443236858098 2118 38-39 -0.23206004584223905 2118 40-41 -0.9765399612535788 2118 42-43 -0.23954692208557304 2118 44-45 -0.04416716921468122 2118 46-47 -0.7099800681839596 2118 48-49 -1.0448672473325331 2118 50-51 -0.546323574915391 2118 52-53 -0.9606499704048019 2118 54-55 0.07380243635646622 2118 56-57 0.8155122952402465 2118 58-59 0.8836619666691377 2118 60-61 -1.9344330535886876 2118 62-63 0.24140780186250055 2118 64-65 -0.22040220046527992 2118 66-67 -2.43380318498383 2118 68-69 1.0179825178071482 2118 70-71 -0.9507002268075304 2118 72-73 -1.6347783437493248 2118 74-75 0.12760346128918343 2118 76 -0.6413541842189296 2119 1 0.9902898426294193 2119 2 0.9054058716865185 2119 3 0.32908951259302555 2119 4 0.2232667862058797 2119 5 0.5115347852491396 2119 6 1.042092863809522 2119 7 -1.8213655553873451 2119 8 0.7380201399557649 2119 9 -0.2372676106640057 2119 10-11 0.3176891442370575 2119 12-13 0.4900576190160777 2119 14-15 -0.5161146213994598 2119 16-17 0.07058082961918188 2119 18-19 -0.37377917633896374 2119 20-21 -0.8555762281882835 2119 22-23 0.7370506417946316 2119 24-25 0.36109352234277736 2119 26-27 0.5174664882367708 2119 28-29 0.5791800706741341 2119 30-31 -0.04036341325394943 2119 32-33 0.676348121839645 2119 34-35 0.40663791776876934 2119 36-37 -0.4774807284437159 2119 38-39 -0.3695202569328728 2119 40-41 -0.19238665063016924 2119 42-43 -0.5101766571984783 2119 44-45 -1.0630238546821928 2119 46-47 -0.21210214335008715 2119 48-49 -0.4986190389968712 2119 50-51 0.540262676271297 2119 52-53 -1.277769904881076 2119 54-55 -1.5850925953815924 2119 56-57 0.8281982095009681 2119 58-59 1.0968653694352852 2119 60-61 0.625087431882541 2119 62-63 -0.07441841135021576 2119 64-65 0.3482301779342265 2119 66-67 0.8055445561229959 2119 68-69 -0.5451771404270573 2119 70-71 -1.027839520566026 2119 72-73 -2.3861418701170365 2119 74-75 -0.03450260523973725 2119 76 0.6864878142546615 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 20.0 13 147.0 14 478.0 15 879.0 16 1211.0 17 1453.0 18 1294.0 19 1380.0 20 1277.0 21 1206.0 22 1216.0 23 1291.0 24 1410.0 25 1635.0 26 1979.0 27 2441.0 28 2887.0 29 3594.0 30 4513.0 31 5528.0 32 7119.0 33 9375.0 34 13248.0 35 19501.0 36 32553.0 37 39173.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.721809726896694 23.267091436939598 12.961488066181948 28.049610769981758 2 17.89884509179958 19.981633707248946 38.08199784451148 24.037523356439998 3 16.868291998546 27.227391284939 29.39754733465554 26.506769381859456 4 12.559865824027957 18.839479876792787 38.87213106390577 29.728523235273485 5 13.087896740620755 36.93219139207571 35.41314592912397 14.566765938179568 6 26.097354105950547 40.27383632317022 17.73176284524485 15.89704672563437 7 25.18095262389276 31.785803110790834 23.5075792843523 19.525664980964102 8 21.776173561466496 37.27719709965627 19.763533980830182 21.18309535804705 9 23.3691943702211 16.398931183796847 22.346931617445428 37.88494282853663 10-11 24.507203030438305 26.11010847591656 27.15596681312935 22.226721680515787 12-13 23.211040182642577 23.87426742087508 26.527176373805073 26.38751602267727 14-15 21.259940437092258 25.45580929666027 24.004999713026677 29.2792505532208 16-17 22.161993252938288 27.878820730952942 26.078860269499838 23.880325746608932 18-19 21.534159391361467 27.110688799750015 26.743044085479788 24.612107723408734 20-21 20.950328106167376 27.768814289996108 26.463723383224174 24.81713422061234 22-23 22.50062177553584 26.670981895171835 26.837426423228262 23.990969906064066 24-25 21.878846239692876 27.858732598256474 26.34989063127754 23.912530530773108 26-27 20.53453564527546 28.31406360604302 27.206665433744238 23.94473531493728 28-29 21.757998584264936 27.26374123934213 28.31438246529217 22.663877711100767 30-31 21.630773743853986 27.059671319885975 27.499378224464156 23.81017671179588 32-33 20.672920559406666 28.178229565905017 27.24588512138972 23.9029647532986 34-35 22.03221753853414 27.78794584494512 27.244290825143963 22.93554579137677 36-37 22.162312112187436 27.28797454227755 27.060627897633427 23.489085447901587 38-39 21.935922045290766 27.65147408630882 27.50511769094886 22.90748617745155 40-41 21.63555663259124 26.739855492988283 27.84757252453622 23.777015349884252 42-43 21.482504192999127 26.643878858994064 28.084484946654847 23.789132001351962 44-45 20.495953676128284 26.38879145967387 28.064077954709234 25.051176909488614 46-47 21.229329949173838 26.364558156738454 28.46424631239278 23.94186558169493 48-49 20.34959728076832 26.453201028002223 29.301251841412164 23.895949849817296 50-51 20.284549993941674 26.725506826776524 28.251567193209574 24.738375986072228 52-53 19.947196908340718 26.641646844250012 28.65779387662698 24.753362370782288 54-55 19.74663444062522 27.25895835060488 28.407170506794895 24.587236701975016 56-57 19.578595616323042 26.97166616712051 28.560222946387004 24.88951527016944 58-59 19.704226160488236 27.102717318521258 27.74617528330644 25.44688123768406 60-61 20.125120369366552 26.47041942745633 27.949926343513447 25.45453385966367 62-63 19.633120547927735 26.467549694213982 28.26081411143493 25.638515646423354 64-65 20.538043097016114 26.57596183892506 27.440070404122213 25.44592465993661 66-67 20.7791006893737 26.874414096129684 26.681823109642945 25.664662104853676 68-69 20.109496266158192 26.713390175308817 26.95859293790535 26.218520620627643 70-71 20.972329394358745 26.826904068006304 26.477753190186785 25.72301334744817 72-73 21.08775644255113 26.37603708970786 26.115847942401267 26.420358525339743 74-75 20.983489468079 26.52271234431697 26.24689909380201 26.24689909380201 76 21.054913939888653 26.253595138034168 26.130515467862175 26.560975454215 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 0.5 6 0.0 7 0.5 8 1.0 9 0.5 10 1.0 11 1.5 12 1.0 13 0.5 14 2.5 15 4.0 16 6.5 17 10.0 18 16.5 19 25.5 20 33.5 21 39.0 22 55.0 23 92.0 24 130.5 25 148.0 26 262.5 27 464.5 28 660.5 29 769.0 30 920.0 31 1266.5 32 1845.0 33 2228.0 34 2654.0 35 3476.0 36 4269.0 37 4666.0 38 5349.0 39 6772.5 40 8305.5 41 9698.5 42 10299.0 43 10718.0 44 11485.5 45 11869.0 46 11904.0 47 12051.5 48 11656.0 49 10476.0 50 9839.0 51 9181.5 52 7615.5 53 6170.5 54 5634.0 55 5239.5 56 4145.0 57 3015.0 58 2585.0 59 2157.5 60 1499.0 61 1044.0 62 820.0 63 685.0 64 456.5 65 308.0 66 211.5 67 170.0 68 148.0 69 86.5 70 48.5 71 50.0 72 43.5 73 32.5 74 25.0 75 22.0 76 18.5 77 18.5 78 19.0 79 16.0 80 11.0 81 10.0 82 10.0 83 6.0 84 7.0 85 5.5 86 2.5 87 2.0 88 1.0 89 1.0 90 2.0 91 1.5 92 1.0 93 3.0 94 3.5 95 2.5 96 3.0 97 2.0 98 0.5 99 3.5 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7123315626016363 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 156809.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.24548973592077 #Duplication Level Percentage of deduplicated Percentage of total 1 75.46688466363163 34.145361554502614 2 7.711172814275042 6.977915808403854 3 4.524376664928329 6.141229138633624 4 2.873895333267558 5.201232072138716 5 1.8745859702039493 4.240828013698193 6 1.3995968935432495 3.7995268128742614 7 0.9048753329856657 2.865906931362358 8 0.7737952613849385 2.8008596445357092 9 0.6709044524940451 2.731986046719257 >10 3.729439456511015 29.065295997040984 >50 0.06906369363909287 1.9654484117620799 >100 0.0014094631354916911 0.0644095683283485 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.007014903481305282 0.0 2 0.0 0.0 0.0 0.010203495972807683 0.0 3 0.0 0.0 0.0 0.011478932969408644 0.0 4 0.0 0.0 0.0 0.014667525460911044 0.0 5 0.0 0.0 0.0 0.015942962457512005 0.0 6 0.0 0.0 0.0 0.015942962457512005 0.0 7 0.0 0.0 0.0 0.016580680955812485 0.0 8 0.0 0.0 0.0 0.017218399454112965 0.0 9 0.0 0.0 0.0 0.03571223590482689 0.0 10 0.0 0.0 0.0 0.047828887372536016 0.0 11 0.0 0.0 0.0 0.052930635358939854 0.0 12 0.0 0.0 0.0 0.05739466484704322 0.0 13 0.0 0.0 0.0 0.06313413133174754 0.0 14 0.0 0.0 0.0 0.06823587931815138 0.0 15 0.0 0.0 0.0 0.0707867533113533 0.0 16 0.0 0.0 0.0 0.07333762730455522 0.0 17 0.0 0.0 0.0 0.07971481228756003 0.0 18 0.0 0.0 0.0 0.0905560267586682 0.0 19 0.0 0.0 0.0 0.09438233774847107 0.0 20 0.0 0.0 0.0 0.10394811522297827 0.0 21 0.0 0.0 0.0 0.11096301870428356 0.0 22 0.0 0.0 0.0 0.112876174199185 0.0 23 0.0 0.0 0.0 0.11861564068388931 0.0 24 0.0 0.0 0.0 0.12626826266349508 0.0 25 0.0 0.0 0.0 0.13328316614480037 0.0 26 0.0 0.0 0.0 0.13774719563290372 0.0 27 0.0 0.0 0.0 0.1485884101040119 0.0 28 0.0 0.0 0.0 0.16644452805642534 0.0 29 0.0 0.0 0.0 0.18238749051393735 0.0 30 0.0 0.0 0.0 0.2123602599340599 0.0 31 0.0 0.0 0.0 0.23914443686268008 0.0 32 0.0 0.0 0.0 0.2844224502420142 0.0 33 0.0 0.0 0.0 0.3194969676485406 0.0 34 0.0 0.0 0.0 0.3622241070346728 0.0 35 0.0 0.0 0.0 0.42982226785452365 0.0 36 0.0 0.0 0.0 0.5573659675146196 0.0 37 0.0 0.0 0.0 0.8258454552991219 0.0 38 0.0 0.0 0.0 1.1140942165309389 0.0 39 0.0 0.0 0.0 1.5541199803582701 0.0 40 0.0 0.0 0.0 1.9705501597484838 0.0 41 0.0 0.0 0.0 2.560439770676428 0.0 42 0.0 0.0 0.0 3.1503293816043723 0.0 43 0.0 0.0 0.0 3.8148320568334726 0.0 44 0.0 0.0 0.0 4.470406673086366 0.0 45 0.0 0.0 0.0 5.134909348315467 0.0 46 0.0 0.0 0.0 5.846603192418803 0.0 47 0.0 0.0 0.0 6.529599704098617 0.0 48 0.0 0.0 0.0 7.1711445133889 0.0 49 0.0 0.0 0.0 7.738076258378027 0.0 50 0.0 0.0 0.0 8.31393606234336 0.0 51 0.0 0.0 0.0 8.92423266521692 0.0 52 0.0 0.0 0.0 9.561313445019099 0.0 53 0.0 0.0 0.0 10.157580240930049 0.0 54 0.0 0.0 0.0 10.71749708243787 0.0 55 0.0 0.0 0.0 11.290806012410002 0.0 56 0.0 0.0 0.0 11.795241344565682 0.0 57 0.0 0.0 0.0 12.316895076175475 0.0 58 0.0 0.0 0.0 12.826432156317559 0.0 59 0.0 0.0 0.0 13.362753413388262 0.0 60 0.0 0.0 0.0 13.834665102130618 0.0 61 0.0 0.0 0.0 14.368435485208119 0.0 62 0.0 0.0 0.0 14.925163734224439 0.0 63 0.0 0.0 0.0 15.481254264742457 0.0 64 0.0 0.0 0.0 16.01502464781996 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATCTAA 15 0.002222156 69.99331 6 AGACCGC 15 0.002222156 69.99331 14 TGTGTCC 15 0.002222156 69.99331 8 TGTAACG 15 0.002222156 69.99331 33 GGACCAT 20 7.911064E-5 69.9933 27 CGGCGGC 30 8.351262E-6 58.327755 9 GTTGGCG 30 8.351262E-6 58.327755 57 ATGGACC 20 0.0069435285 52.494976 25 GACCGCA 20 0.0069435285 52.494976 15 GACCATG 20 0.0069435285 52.494976 28 AGGCCCG 20 0.0069435285 52.494976 21 GAGCACC 20 0.0069435285 52.494976 67 GCAGCTA 20 0.0069435285 52.494976 24 CGCAACC 20 0.0069435285 52.494976 18 AGTTGGC 35 2.0803356E-5 49.995216 56 GCGGCTT 35 2.0803356E-5 49.995216 11 ATATTAG 35 2.0803356E-5 49.995216 51 CGGATGC 30 5.866563E-4 46.662205 9 GGCGGCG 30 5.866563E-4 46.662205 7 CGTCAAA 30 5.866563E-4 46.662205 65 >>END_MODULE